THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST最新文献

{"title":"Uji Toksisitas Akut Kulit Kentang (Solanum tuberosum L.) Terhadap Larva Artemia salina Leach Dengan Metode BSLT (Brine Shrimp Lethality Test)","authors":"Baterun Kunsah","doi":"10.30651/jmlt.v2i2.3090","DOIUrl":"https://doi.org/10.30651/jmlt.v2i2.3090","url":null,"abstract":"Tanggal Submit:28 Januari 2019 Tanggal Review:30 April 2019 Tanggal Publish Online:17 Mei 2019 Potatoes are one of the vegetables that contain substances that are important for the formation of body tissues, such as protein and fat. Potatoes also form red blood cells or hemoglobin (Ca, P and Fe). In Indonesia tend to process potatoes using only meat. The skin is removed, even though the nutritional content of the skin is five times greater than the meat. Potato peels that supply querestin, antioxidants and flavonoids that act as free radical acceptors. (Free radicals are reactive molecules that cause damage to the body that can lead to diseases such as heart disease and cancer) in the skin of potatoes found also effective antioxidants, chlorogenic acid. Potato skin is also proven to increase hemoglobin levels (Khomsan, A, 2009). However, that does not mean potato skin does not have adverse side effects, if the use is not appropriate. In order to use it optimally, you need to know enough information about the advantages and disadvantages and the possibility of abuse. However, the level of toxicity from potato skin is still unknown. Brine Shrimp Lethality Test (BSLT) is one of the oxicity test methods that is widely used in tracing bioactive compounds that are toxic from natural materials. This research is an experimental research with post test-only control group design approach. Treatment with giving potato skin juice to Artemia salina Leach larvae with the aim to determine the potential of plant biological activity based on the toxicity of secondary metabolites contained in it, and at the same time as the initial screening test of anticancer activity of chemical compounds in potato skin juice. The results showed the LC50 value was at a concentration of 0.5% to 1% and based on the results of SPSS 21 analysis with probit analysis obtained LC50 price data of 0.746 gr / ml (7460 μgr / ml), based on the results of research and data analysis it can be concluded that potato skin has an LC50 value at a concentration of 0.746 gr / ml (7460 µgr / ml). With an LC50 price of more than 1000 µg / ml according to the BST method the potato skin does not have the potential for toxicity so the skin of the potato has the potential as an alternative to increasing hemoglobin levels.Keywords: potato skin, toxicity, BSlT","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"148 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80596429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pemeriksaan Jumlah Kapang Sebagai Indikator Kualitas Terasi Di Pasar Tambaksari Surabaya","authors":"Dita Artanti","doi":"10.30651/jmlt.v2i2.3387","DOIUrl":"https://doi.org/10.30651/jmlt.v2i2.3387","url":null,"abstract":"The main problem that occurs in the community is the lack of awareness of the importance of hygiene during the manufacture and storage of shrimp paste. This can trigger one of the contaminations originating from microorganisms including mold. The aimed of this study was to determine the number of molds as an indicator of shrimp paste quality in the Tambaksari market in Surabaya. This research is descriptive. The population of this research is shrimp paste traders in Tambaksari market in Surabaya. The research variable is the number of molds in the shrimp paste in the packaging without a brand in the Tambaksari market in Surabaya. Data on the number of molds was tabulated and analyzed descriptively by calculating the percentage of samples that were of good quality and not good. The results of the research on the number of mold colonies on shrimp paste sold in the Tambaksari market in Surabaya showed that the number of mold colonies in shrimp paste samples was safe to consumed because the amount was <2 x 102 colonies / gr, the average percentage of all shrimp paste samples was 100% good quality. The highest growth rate of mold colonies was 19 x 101 colonies / gr and the lowest growth rate of mold colonies was 0 x 101 colony / gr. Keywords : The Number of  Mold, Shrimp paste","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81339800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analisis Kadar Trigliserida dan HDL (High Density Lipoprotein) Pada Tenaga Farmasi di Apotek Indobat Denpasar","authors":"A. Indrayani","doi":"10.30651/jmlt.v2i2.2581","DOIUrl":"https://doi.org/10.30651/jmlt.v2i2.2581","url":null,"abstract":"AbstractNight shift can cause health problems one of which is the body's circadian rhythm disturbance which can lead to increased triglyceride levels and decreased levels of HDL (High Density Lipoprotein). This study to find out differences in triglyceride and HDL levels in pharmacist before and after night shift in Apotek Indobat Denpasar. This study method is pre-experimental one group pretest-posttest design. Blood collection was carried out on 28 pharmacists before and after the night shift. Triglyceride levels were measured using the GPO-PAP (Glycerol Peroxidase Phosphate Acid) method and HDL measured using the CHOD-PAP (Cholesterol Oxidase Diaminase Peroxidase Aminoantipyrine) method with a chemistry analyzer. The average triglyceride levels before the night shift were 87.05 ± 0.53 mg/dL, and levels after the night shift were 105.77 ± 0.50 mg/dL with a percentage increase in triglyceride levels was 24.66%. The average HDL levels before the night shift were 59.84 ± 0.47 mg/dL, and the levels after the night shift were 48.61 ± 0.42 mg/dL with a percentage decrease in HDL levels was 27.24%. Based on the results of the study, the night shift can increase triglyceride levels and decrease HDL levels. Keywords: chemistry analyzer, CHOD-PAP, HDL, GPO-PAP, pharmacist, triglycerides.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87437952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efektivitas Vitamin C Pada Daun Kelor Terhadap Bilangan Peroksida Dari Minyak Jelantah","authors":"Nastiti Kartikorini","doi":"10.30651/jmlt.v2i2.3380","DOIUrl":"https://doi.org/10.30651/jmlt.v2i2.3380","url":null,"abstract":"Used cooking oil is a cooking oil that has been used several times. Used cooking oil undergoes an oxidation reaction to produce high peroxide compounds. Phenolic compounds are primary antioxidants contained in Moringa leaves which slow the oxidation down in used cooking oil so that it can be stored longer. \u0000Formulation of the problem in this study was whether there was an inhibitory test on Moringa oleifera Leaves powder on the peroxide value in used cooking oil. The purpose of this study was to determine the inhibition of Moringa leaves powder to peroxide value on used cooking oil. This type of research was an experimental i.e. to determine whether there was the inhibition of Moringa leaves powder to the peroxide value in used cooking oil. The sample in this study was 5 samples from 6 fried traders, and repeated 5 times so that the total sample was 25 samples. Peroxide levels could be decreased due to the antioxidant content found in Moringa leaves. Antioxidants found in Moringa leaves are phenolic components. Phenolic components can inhibit free radicals and inhibit peroxide value to decrease. Moringa was suitable for areas where strong and long winds, drought occur simultaneously, and cause serious soil erosion. Nutrient content in deciduous leaves could fertilize and improve marginal soil quality (Dudi, 2015). Therefore, Moringa leaves not only have high antioxidants but also could be found easily in Surabaya. \u0000From the results of peroxide levels test, it was known that the average peroxide value in used cooking oil without Moringa leaves was equal to 23,9957 mEq and with Moringa leaves by different time variation i.e. 1 day (14,3182 mEq), 2 days (10,7987 mEq), 3 days (6,1593 mEq), 4 days (3,0397 mEq). Statistical analysis with Anova test showed the inhibition of Moringa leaves powder to decrease in peroxide value on used cooking oil with a value of p (sig) = 0,000 which was less than 0.05. Based on the results of the Tukey HSD test, the most effective soaking of Moringa leaves powder for 4 days was to reduce peroxide levels in used cooking oil by giving 5gr Moringa leaf powder with 100ml used cooking oil. \u0000Keywords : Levels of Peroxide Value, Moringa Leaves, Used Cooking Oil","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87920100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HUBUNGAN FREKUENSI MENGURAS TERHADAP PERTUMBUHAN JAMUR PADA AIR BAK TOILET TEMPAT WISATA DI WILAYAH KOTA KEDIRI","authors":"Durroh Humairoh, Endrik Asmarani","doi":"10.30651/jmlt.v2i2.2449","DOIUrl":"https://doi.org/10.30651/jmlt.v2i2.2449","url":null,"abstract":"Tempat wisata merupakan suatu tempat menarik yang dituju bersama rekan, teman, ataupun keluarga untuk berlibur. Setiap tempat wisata selalu dilengkapi toilet yang bersih demi kenyamanan pengunjung. Pada tempat wisata yang menyediakan toilet umum harus selalu dibersihkan dengan teratur supaya tidak terinfeksi oleh mikroorganisme yang dapat membahayakan kesehatan. Pada penelitian ini, dilakukan penelitian pada beberapa toilet tempat wisata di wilayah kota Kediri. Metode yang dilakukan pada penelitian ini dengan centrifugasi sebanyak 15 tempat wisata di kota Kediri. Hasil penelitian dengan uji korelasi spearman’s rho menunjukkan terdapat hubungan frekuensi menguras dengan jumlah jamur yang tumbuh pada air bak toilet dan hasil tersebut menunjukkan bahwa semakin lama masa frekuensi menguras bak toilet, maka semakin tinggi pertumbuhan jamur yang muncul di air bak toilet tersebut. Kata kunci: frekuensi menguras, pertumbuhan kapang, tempat wisata","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88862146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GAMBARAN PEMERIKSAAN KADAR GLUKOSA DAN KOLESTEROL PADA LANSIA","authors":"Nur Vita Purwaningsih, Fitrotin Azizah, Ellies Tunjung Sari Maulidiyanti, Dita Artanti","doi":"10.30651/jmlt.v2i2.10872","DOIUrl":"https://doi.org/10.30651/jmlt.v2i2.10872","url":null,"abstract":"The increasing life expectancy based on WHO indicates an increase in the number of elderly people (elderly) so that there is an increase in health problems in the elderly due to the aging process which causes many changes in the body of the elderly. One of the changes in the body of the elderly is the blood glucose and cholesterol regulation system, resulting in an increase of more than normal. The purpose of this study was to determine the profile of glucose and cholesterol levels in the elderly. This research is observational. The population and sample in this study were the elderly in Karang Penang. The examination method used in this examination is the POCT (Point Of Care Test) examination method. The results showed that the elderly who had levels as much as 10 respondents (20%) samples had normal glucose levels, while as many as 40 respondents (80%) samples had high glucose. For cholesterol levels, 20 respondents (40%) samples had normal cholesterol levels, while 13 respondents (26%) samples had threshold cholesterol levels, then 7 respondents (14%) samples had high cholesterol levels. Keywords        : blood glucose, cholesterol and the elderly","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78689275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uji Efektivitas Formula Pedikulosida Berbahan Aktif Minyak Atsiri Terhadap Mortalitas Kutu Kepala (Pediculus humanus capitis De Geer) Secara In Vitro","authors":"Tanendri Arrizqiyani","doi":"10.30651/JMLT.V2I1.1921","DOIUrl":"https://doi.org/10.30651/JMLT.V2I1.1921","url":null,"abstract":"Pediculosis is a disease caused by Pediculus humanus capitis de Geer or often called head lice which is an obligate ectoparasite in the human head. The prevalence of pediculosis is quite high and there are reports of resistance and negative side effects regarding the use of synthetic pediculosides trigger research and development of vegetable pediculosides. This study aims to examine the effectiveness of the formula of pediculoside with active ingredients of essential oils on head lice mortality in vitro. The study was conducted by testing 3 formulas with variations in the composition of essential oils made from cassava oil, fennel oil, lemon oil, water and vegetable glycerine. Comparison of the ingredients in formula A is 10%, 10%, 5%, 50% and 20%; formula B is 5%, 15%, 5%, 50% and 20%; while formula C is 15%, 5%, 5%, 50% and 20%. The test method is an experiment with a completely randomized design. The test is done by placing 10 adult head lice on filter paper which has been saturated with a formula made in a petri dish. Mortality of head lice is observed based on time. Test results data will be compared with negative controls and positive controls in the form of testing using permethrin-active pediculoside drugs. The results showed that the pediculoside formula A, B and C had a high mortality rate which caused 100% of the deaths of head lice tested in less than 5 minutes. Statistically there is a significance to the results of testing formulas A, B, and C when compared to controls. Based on the results of multiple statistical statistics, it was found that formula C was the most effective as a pediculoside formula","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87097736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efek Pemberian Gentamisin Secara Oral Terhadap Kadar Asam Urat, Ureum, dan Kreatinin Tikus Wistar","authors":"Syahrul Ardiansyah","doi":"10.30651/jmlt.v2i1.2211","DOIUrl":"https://doi.org/10.30651/jmlt.v2i1.2211","url":null,"abstract":"ABSTRACTGentamicin is an aminoglycoside class of antibiotics which at high doses can cause damage to the kidneys. This research was to determine the levels of BUN, creatinine and amino acids after gentamicin induction. This research is experimental with gentamicin test material given to male rats as much as 0.3 ml/day for 7 and 10 days. Blood serum levels were then measured for BUN, creatinine and amino acids using a photometer microlab 300. The results showed that after doing treatment for 7 days the average value of BUN levels increase from 21.7 to 36.3 mg/dl, creatinine increase from 0, 88 to 1.9 mg/dl, uric acid increase from 1.54 to 4.29 mg/dl. It was concluded that giving of gentamicin as much as 0.3 ml/day for 7 and 10 days increased BUN levels, creatinine and uric acid exceeded the normal limit. Keywords : Gentamicin, urea, creatinine, uric acid, rat.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"113 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91448732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analisa Asam Salisilat Pada Perlengkapan Persedian Bermerk Bayi Di Pasar Blauran Surabaya","authors":"Nastiti Kartikorini","doi":"10.30651/JMLT.V2I1.2231","DOIUrl":"https://doi.org/10.30651/JMLT.V2I1.2231","url":null,"abstract":"Salicylic acid is a substance that is often added to skin care products for acne. In the regulation of the National Agency of Drug and Food Control No. Hk.03.1.23.08.11.07517 in 2011 concerning the technical requirements of cosmetics, salicylic acid levels are limited to 3% for rinse production and 2% for other production and are not used for children under 3 years. Using at high doses can cause the baby to bleed, mute and deaf. This is because the structure of the baby's skin is still thin, so it becomes susceptible to irritation and infection. The title of this research is Analysis of Salicylic Acid in the mains supplies barnded baby in the market blauran in Surabaya Region. The purpose identified salicylic acid content with qualitative tests on baby cosmetics circulating in Surabaya. The formulation of this problem is the presence or absence of Salicylic Acid in baby cosmetics. The type of research used descriptive, there were 26 baby cosmetics products in 4 types of products in the form of powder, soap, shampoo, and lotion which were conducted in the Laboratory of Muhammadiyah University of Surabaya in June 2018. Based on the results that found from 26 samples of baby cosmetics in the form of powder as much as 100% of soap, shampoo and lotions sold in Surabaya were not found for Salicylic Acid. So that all samples met the requirements based on the regulation of the National Agency of Drug and Food Control No.Hk.03.1.23.08.11.07517 in 2011 concerning the technical requirements of cosmetics materials, salicylic acid levels were limited to 3% not used for children under 3 years.           Keywords: Baby supplies, Salicylic Acid","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85418683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gambaran Bakteri Kontaminan pada Sikat Gigi","authors":"Perdina Nursidika, P. Naully, Linda Lestari","doi":"10.30651/JMLT.V2I1.1804","DOIUrl":"https://doi.org/10.30651/JMLT.V2I1.1804","url":null,"abstract":"Abstract: DESCRIPTION OF BACTERIAL CONTAMINTANT IN TOOTHBRUSH. Brushing activity is an effort to keep oral hygiene from pathogenic microorganisms that can cause infection. Oral infection has been an increasingly common risk factor for systemic disease. Oral infections, especially periodontitis, can affect travel and pathogenesis of a number of systemic diseases, such as cardiovascular disease, bacterial pneumonia, diabetes mellitus, and low birth weight. Keeping your teeth and mouth clean is not enough just brushing your teeth, but replace the toothbrush for a maximum of three months. This study aims to identify the contaminant bacteria found on toothbrushes that have been used for more than 3 months. The method of identification by planting the isolation result of 20 toothbrushes has been used for more than three months on selective media ie blood agar (AD), McConkey Agar (MCA), Eosin Methylen Blue (EMB), Manitol Salt Agar (MSA) . The results showed that all samples of toothbrush were contaminated with Streptococcus and Staphylococcus, 16 samples were Klebsiella (80%), four samples (20%) were Proteus, and one sample (5%) was contaminated with Escherichia. All of the toothbrush samples showed to have been contaminated by bacteria. Bacterial contamination of the toothbrush can be caused by the storage of a toothbrush that is too close to the toilet and toilet, possibly exposed through aerosols containing intestinal bacteria. This toothbrush contamination plays an important role in both oral and systemic diseases, including septicemia and gastrointestinal, respiratory cardiovascularmy, and kidney problems. Abstrak: GAMBARAN BAKTERI KONTAMINAN PADA SIKAT GIGI. Kegiatan menyikat gigi merupakan upaya untuk menjaga kebersihan gigi dan mulut dari mikroorganisme pathogen yang dapat menyebabkan infeksi. Infeksi mulut telah menjadi faktor risiko yang semakin umum untuk penyakit sistemik. Infeksi mulut, terutama periodontitis, dapat mempengaruhi patogenesis dari sejumlah penyakit sistemik, seperti penyakit kardiovaskular, pneumonia bakteri, diabetes mellitus, dan berat badan lahir rendah. Menjaga kebersihan gigi dan mulut tidak cukup hanya menyikat gigi saja, melainkan mengganti sikat gigi maksimal tiga bulan sekali. Penelitian ini bertujuan untuk mengidentifikasi bakteri kontaminan yang terdapat pada pada sikat gigi yang telah digunakan selama lebih dari 3 bulan. Metode identifikasi dengan melakukan penanaman hasil isolasi 20 sikat gigi yang telah digunakan selama lebih dari tiga bulan pada media selektif yaitu agar darah (AD), McConkey Agar (MCA), Eosin Methylen Blue (EMB), Manitol Salt Agar (MSA) dengan metode gores.  Hasil penelitian menunjukkan semua sampel sikat gigi terkontaminasi Streptococcus dan Staphylococcus, 16 sampel terdapat Klebsiella (80%), pada empat sampel (20%) terdapat Proteus, dan satu sampel (5%) terkontaminasi Escherichia. Semua sampel sikat gigi menunjukkan telah terkontaminasi oleh bakteri. Kontaminasi bakteri pada sikat gigi ini d","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90799718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}