THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST最新文献

{"title":"Gambaran Anti-Plasmodium sp. Pada Anggota Tentara Nasional Indonesia Angkatan Darat Di Kota Cimahi","authors":"P. Naully, Gina Khairinisa, D. Saputri","doi":"10.30651/jmlt.v2i1.1834","DOIUrl":"https://doi.org/10.30651/jmlt.v2i1.1834","url":null,"abstract":"Malaria is a disease caused by Plasmodium sp. The presence of such parasites in body can be characterized by the presence of anti-Plasmodium sp. antibodies. Malaria cases are still prevalent in Indonesia and generally occur in people who travel to endemic areas. One of the jobs that obliges its members to serve to various regions, including endemic areas is the Indonesian Army (TNI AD). Many Army personnel residing in Cimahi City. The purpose of this study is to determine the overview of malaria in members of the Army in Cimahi City. This study used a sample of 30 TNI AD who are residing in Cimahi City and have been assigned to various regions in Indonesia. Venipucture is only performed on respondents who have signed informed consent and filled out the interview form. Malaria screening is performed using immunochromatography that can detect anti-P. vivax and anti-P. falciparum antibodies, with a high degree of sensitivity and specificity. Of the 30 people, there were 4 people (13.3%) who had been infected with malaria and 2 (6.7%) of whom had experienced mixed malaria. All respondents who tested positive for malaria claimed to have been assigned to malaria endemic areas such as Papua and Maluku for several years.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76056879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perbedaan Zona Hambat Terhadap Jamur Malassezia Furfur Antara Pemberian Ekstrak Umbi Bawang Putih (Allium Sativum Linn) Dengan Ekstrak Kulit Umbi Bawang Putih (Allium Sativum Linn)","authors":"D. Ariana","doi":"10.30651/jmlt.v1i2.1501","DOIUrl":"https://doi.org/10.30651/jmlt.v1i2.1501","url":null,"abstract":"Tinea versicolor or pitiriasis versicolor is a skin disorder caused by Malassezia furfur infection which is a fungal infection on the skin of the chronic surface. Clinical features is in the form of fine spots that colored white to brown black, this infection is also called a skin fungus with blotches. Treatment of Tinea versicolor can use chemical or traditional medicine. Chemical treatments such as creams or ointments cause sticky taste, while one of the traditional medicine is the bulb and skin of the garlic bulb (Allium sativum Linn) containing allicin substances that are efficacious as an anti-fungal.               This research is an experimental research with the aim to know the difference of inhibition zone to Malessezia furfur fungus. There are 3 treatments with 9 repetitions. The extraction method used is the soxhlet method. Anti-fungal activity test was done by using rating method, Saboroud Dextrose Agar (SDA) media which has been inoculated with suspension of Malassezia furfur with concentration 1.5x108 CFU/ml (Mc Farland standard).               Result of data analysis obtained by ANOVA p = 0,000 < α = 0,05, that there is difference of inhibition zone from treatment of garlic bulb extract and skin of garlic bulb (Allium sativum Linn). It is indicated that there is effect of giving garlic bulb extract (Allium sativum Linn) and garlic bulb skin (Allium sativum Linn) to Malassezia furfur fungus. Further tests were performed to compare mean treatment i.e. HSD test. It was obtained sig (p) 0,016 > α 0,05 which means bulb extract and garlic bulb skin (Allium sativum Linn) have different effect. Keywords: Tinea versicolor, Malassezia furfur, garlic bulb.","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"98 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80538434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perbedaan Pertumbuhan Bakteri Shigella dysentriae Pada Berbagai Konsentrasi Perasan Kulit Apel Manalagi (Malus sylvestris Mill) Secara In Vitro","authors":"Dita Artanti","doi":"10.30651/jmlt.v1i2.1483","DOIUrl":"https://doi.org/10.30651/jmlt.v1i2.1483","url":null,"abstract":"Apple manalagi is one type of apple that is consumed by many Indonesian people, because it tastes sweet, tasty, easy to get and the price is quite affordable. The Manalagi apple rind contains some phytochemical derivatives of polyphenols that have antibacterial effects. Shigella dysentriae is a bacillus bacteria that causes acute diarrhea (dysentery) in most people who lack hygiene. One alternative to prevent the disease is to use natural ingredients as an antibacterial. The aim of this research is to know the different growth of S. dysentriae bacteria in various concentrations of Manalagi apple rind (Malus sylvestris Mill.) In vitro. The type of this study was experimental consisting of 7 treatments with 4 repetitions. The sample consisted of 7 concentrations of manalagi apple rind (100%, 50%, 25%, 12.5%, 6.25%, 3.125%, and 0% (control) .The data analysis used ANOVA test with 5% 0.05). The results showed that the growth of S.dysentriae bacteria was only at 0% concentration; 3.125%; 6.25%; 12.5%; and 25%. While at concentrations of 50% and 100% there was no growth of S.dysentriae bacterial colonies. The conclusion of this study proves that Manalagi apple rind has antibacterial power against S.dysentriae bacteria.Keywords: Shigella dysentriae, Manalagi Apple Rind (Malus sylvestris Mill).","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84861547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Respon Inflamasi Pada Perokok Pasif Di Kecamatan Pahandut Kota Palangka Raya Ditinjau Dari Jumlah Leukosit Dan Jenis Leukosit","authors":"Rinny Ardina","doi":"10.30651/JMLT.V1I2.1495","DOIUrl":"https://doi.org/10.30651/JMLT.V1I2.1495","url":null,"abstract":"Proporsi perokok pasif di Indonesia mencapai 40,5 persen dan 78,4 persen perokok pasif banyak terpapar asap rokok di dalam rumah.Setiap hembusan asap rokok mengandung 1015 radikal bebas oksidatif dan nikotinpenyebab leukositosis di dalam darah sebagai bentuk respon inflamasi.Jenis leukosit penunjuk inflamasi akibat asap rokok diawali neutrofilia, neutropenia, limfositosis, limfopenia dan monositosis sebagai bentuk respon inflamasi. Analisis deskriptif dengan rancangan cross sectional digunakan untuk memberikan gambaran respon inflamasi ditinjau dari jumlah leukosit dan jenis leukosit pada perokok pasif yang tinggal di kecamatan Pahandut kota Palangka Raya. Sampel yang diperoleh sebanyak 30 orang diambil dengan teknik Purposive Sampling dimulai dengan observasi, pembagian lembar inform consent, wawancara, pengisian kuesioner, pengambilan sampel darah dan pemeriksaan laboratorium menggunakan hematology analyzer dan apusan darah tepi. Data yang diperoleh dimuat dalam bentuk tabel dan dianalisis dan dideskripsikan dalam bentuk persentase (%). Pada 5 orang (17%) terjadi leukositosis, 2 orang (6%) neutropenia, 1 orang (3%) neutrofilia, 1 orang (3%) limfopenia, dan 6 orang (20%) limfositosis. Leukositosis menunjukkan penanda adanya perubahan imun sistemik, dimana selanjutnya terjadi rekrutmen sel inflamasi diantaranya neutrofil dan limfosit. Radikal bebas dan nikotin memicu inflamasi ditandai dengan neutrofilia dengan limfopenia relatif pada awal inflamasi dan selanjutnya berkembang menjadi limfositosis dengan neutropenia relatif.Proportion of passive smokers in Indonesia reached 40,5% and 78,4% of them were exposure of cigarette smoke in the house. Each puff of cigarette smoke contains 1015 of oxidative free radicals and nicotine that causes leukocytosis in the blood where it found as an inflammatory response. Either free radicals or nicotine can also cause of neutrophilia, neutropenia, lymphocytosis, lymphopenia, and monocytosis. This study aimed to overview of the inflammatory response in terms of white blood cell count and differential counting of leukocytes in passive smokers (housewives) who live in the district of Pahandut, Palangka Raya. Descriptive analysis with cross sectional design was used in this study. Samples obtained by 30 people were taken with Purposive Sampling technique. Procedure of collecting data starts from observation, distribution of informed consent, interview, answered of questionnaire, blood sampling and laboratory examination using hematology analyzer for measured of white blood cell count and peripheral blood smear for measured of differential counting of leukocytes. The data obtained were analyzed and described in percentage (%). This study found 5 people (17%) of leukocytosis, 2 people (6%) of neutropenia, 1 person (3%) of neutrophilia, 1 person (3%) of lymphopenia, and 6 people (20%) of lymphocytosis. Leukocytosis occurs because of the mechanism of leukocytes recruitment into inflammatory tissues and the types of leuko","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"344 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77149241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perbandingan Pemeriksaan Leukosit Urine Segar Dengan Setelah 2 Jam Di Suhu Kamar","authors":"Nur Vita Purwaningsih","doi":"10.30651/jmlt.v1i2.1484","DOIUrl":"https://doi.org/10.30651/jmlt.v1i2.1484","url":null,"abstract":"ABSTRACTBackground: Urinalysis is a parameter often requested by clinicians. The urinalysis parameters consist of macroscopic, microscopic or sedimental examination and urine chemistry examination. Urine examination is very important, especially in making the diagnosis. Procrastination delay results in errors in diagnosis and administration of drugs that lead to adverse outcomes of patients, analysis should be performed no later than 4 hours after sampling. Urine has a stability at room temperature ie for 1 hour, if urine is silenced long then the bacteria will multiply, so it can decompose NH3 (ammonia) which is alkaline. Under alkaline conditions, the pH in the urine will increase. This may affect the sediment component in the urine to rapid lysis so that the amount will be reduced General purpose of this study to determine the difference results of examination of fresh urine leukocytes compared after 2 hours at room temperature. Method: descriptive observasional with number of sample counted 20 sample. Result: Based on the test result, the positive percentage value in fresh urine is 100% and the positive percentage value in urine after 2 hours is 70%. In the Wilcoxon statistical test obtained p-value <0.001 (> 0.05). Conclusion: Based on the research that has been done then it can be concluded that there are differences in the results of fresh urine leukosit better than urine after 2 hours at room temperature.Keywords: Leukocyte urine, Fresh urine, delay. ","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87152036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioaktif Belimbing Wuluh (Averhoa bilimbi (Linn.)) Terhadap Kadar Formalin Dalam Tahu","authors":"Rinza Rahmawati Samsudin","doi":"10.30651/JMLT.V1I2.1541","DOIUrl":"https://doi.org/10.30651/JMLT.V1I2.1541","url":null,"abstract":"This study aims to determine the influence of the juice of belimbing wuluh to the content of formalin in tofu and know the concentration of belimbing wuluh feeling is most effective in reducing the content of formalin in tofu. This study used a complete randomized design. The sample of this study was 30 tofu containing  formalin which was divided into 6 groups. The weight of each sample is 15 grams.The data of the research were analyzed by using anova test with 95% confidence level, then Duncan test with 95% confidence level. From the result of this research can be concluded that there is influence to formalin content in tofu. The content of formaldehyde in the highest to the lowest yields was found in soaking the belimbing wuluh with the concentrations is the group without treatment (8269.40 mg / Kg), 0% (1434.20 mg / Kg), 100% (762.00mg / Kg), 25% (573.80 mg / Kg), 75% (442.20 mg / kg), and 50% (162.00 mg / kg). The highest decrease in formaldehyde content in tofu by 98.40% occurred in the group K3 with the most effective 50% concentration to decrease the formalin content in tofu. Keyword : belimbing wuluh, tofu, formalin","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81877589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hubungan Kadar TSH Terhadap Kadar FT4 Pada Pasien Tiroid Di Bangkalan","authors":"Ellies Tunjung Sari Maulidiyanti","doi":"10.30651/jmlt.v1i2.1487","DOIUrl":"https://doi.org/10.30651/jmlt.v1i2.1487","url":null,"abstract":"AbstractThyroid disorder disease ranks second most in the list of metabolic diseases after diabetes mellitus (DM). Thyroid disease or Thyroid abnormalities is a condition of abnormalities in a person due to the disorder of the thyroid gland. TSH is a primary factor that controls thyroid cell growth and synthesis also thyroid hormone secretion. TSH secretion is stimulated by low levels of T3 and T4 and by the hormone TRH (Thyroid Releasing Hormone) the hypothalamus is inhibited by elevated levels of T3 and T4. T3 and T4 are circulating in the plasma, most of which are bound with proteins, Thyroid Binding Globulin (TBG) and a small part in free form that are Free Triiodotironine (FT3) and Free Thyroxine (FT4). The free hormone (FT3 and FT4) is an actively metabolic fraction which is known to be quantitative. Currently the measurement of free hormone levels should be part of a complete examination of the status of the thyroid gland. The purpose analyzes the levels of TSH, FT4 and proves the relationship between the two in patients with thyroid disorders. This examination by using ELISA method is with EIA competitive principles and sandwiches. The samples are serum of patients with thyroid disorders at RSUD Bangkalan and Farmalab Laboratory from October to November 2017.The 30 samples obtaines an average result of TSH level of 1.9 mIU / L and an average FT4 level of 2.8 ng / dl. Next test Kolmogorov-Smirnov and Spearrman's test results obtained if the variable one value rose (high) then the other variable down (low). It can be concluded that there is an opposite relationship between TSH levels and FT4 levels, if TSH levels increase, the FT4 level decreases, and so it is also in contrast Keywords: TSH, FT4, thyroid disorders","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77166728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gambaran Histologi Ginjal Tikus Wistar Yang Terpapar MSG Setelah Perlakuan Diberikan Jus Tomat Dan Diberhentikan Perlakuan Saja","authors":"Yeti Eka sispita Sari","doi":"10.30651/jmlt.v1i2.1499","DOIUrl":"https://doi.org/10.30651/jmlt.v1i2.1499","url":null,"abstract":"MSG causes many side effects on the body, but MSG has long been used as a food flavor that can bring tastes (umami) and play a role in strengthening the taste. The chemical structure of MSG is no different from Glutamic Acid (glutamate), it is one of the 20 amino acids that make up proteins in the body. So the use of MSG needs to be discontinued to prevent kidney damage due to continued consumption of MSG, two research were conducted to determine the effect of discharging of MSG consumption by being given tomato juice (1) and discharged without treatment (2), the second study was seen from the histology picture of rats wistar mouse.Methods were experimental with post test only control group design. The research (1) used 15 wistar rats divided into three groups: group I without treatment (pellet AD II and drinking water), group II was given MSG for 14 days and group III was given tomato juice and MSG for 14 days. The study (2) used 27 rats divided by 9 groups. The sample is selected by simple random sampling method. The mice were then gradually turned off on the 29th, 43rd and 57th days. There was no significant difference in mean number of normal and damaged proximal tubules in all treatment groups. The renal histologic features in the treatment group I (MSG) and the treatment group II (MSG and tomato juice) showed normal glomeruli, tubular epithelial tubules, and lumen tubular narrowing whereas in mice discharged without treatment there was no apparent difference between the two MSG Giving groups damage to proximal tubules and renal corpusculum and regeneration after 14 days of discontinuation of MSG. Conclusions from these two experiments occurred damage to the renal tubules Keywords: MSG, Kidney, Wistar Rat","PeriodicalId":22695,"journal":{"name":"THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73795067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}