The Journal of Cancer Research最新文献_第7页

A549、MRC-5的有氧糖酵解及养阴扶正汤对A549有氧糖酵解的干预 A549、MRC-5的有氧糖酵解及养阴扶正汤对A549有氧糖酵解的干预

The Journal of Cancer Research Pub Date : 2018-08-30 DOI: 10.12677/WJCR.2018.84023

孙玺媛, 魏冬梅, 陈禹含

引用次数: 0

紫杉醇联合TLR7/8激动剂3M-052诱导小鼠结肠癌CT26细胞凋亡的研究紫杉醇联合TLR7/8激动剂3M-052诱导小鼠结肠癌CT26细胞凋亡的研究

The Journal of Cancer Research Pub Date : 2018-08-30 DOI: 10.12677/WJCR.2018.84021

安稳定, 毛剑琴, 郑子瑞

引用次数: 0

Presence of Lamin A is Required for GATA3 and ERα Downregulation by Histone Deacetylase Inhibitor in Breast Cancer Cells 组蛋白去乙酰化酶抑制剂在乳腺癌细胞中下调GATA3和ERα需要Lamin A的存在

The Journal of Cancer Research Pub Date : 2018-08-01 DOI: 10.12691/jcrt-6-3-1

Callinice D Capo-Chichi, F. Gnangnon, Charles Kuassi Mekpossi, Sara Hounguè, Xiang-Xi Xu, J. Olory-Togbe

{"title":"Presence of Lamin A is Required for GATA3 and ERα Downregulation by Histone Deacetylase Inhibitor in Breast Cancer Cells","authors":"Callinice D Capo-Chichi, F. Gnangnon, Charles Kuassi Mekpossi, Sara Hounguè, Xiang-Xi Xu, J. Olory-Togbe","doi":"10.12691/jcrt-6-3-1","DOIUrl":"https://doi.org/10.12691/jcrt-6-3-1","url":null,"abstract":"Background: Breast cancer treatment is challenging due to the inconsistence in tumour biomarker expression including progesterone receptor (PR), estrogen receptor-α (ERα) and GATA3 transcription factor. GATA3 has role in epithelial cell differentiation along with nuclear envelope protein lamin A. The breast cancer cell line MCF7 expresses ERα, abnormal GATA3 isoforms, low PR but lacks lamin A. MCF7 cells are resistant to tamoxifen targeting ERα and more anticancer drugs are being studied to kill them. One of the mechanisms surrounding breast cancer initiation is histone deacetylation. Our objective is to investigate the effect of histone deacetylase inhibitor (HDACI) on PR, ERα and GATA3 expression along with the induction of apoptosis in MCF7 cells forced expressing lamin A. Subsequently, they are also explored as biomarkers for breast cancer prognostic and indicators for targeted breast cancer therapy. Methods: The HDACI used here is Suberoyl-Bis-Hydroxamic Acid (SBHA). Western blot was used to analyze the expression of PR, ERα, and GATA3 in MCF7 control transfected with histone H2B-GFP (MCF7-H2B-GFP) and in MCF7 transfected with lamin A-RFP (MCF7-LA-RFP). The analyses were carried out before and after treatment with DMSO (mock) or SBHA (1 or 2 µM) for 12h. The in vivo expression of the PR, ERα and GATA3 were also explored in 36 archived cell lysates derived from breast cancer micro-biopsies. Results: MCF7-H2B-GFP treated with SBHA increased PR, ERα while MCF7-LA-RFP treated with SBHA reduced ERα and GATA3 but not PR. Biomarkers analysis in ductal carcinoma micro-biopsies derived samples showed that 30% had lost lamin A while 64% expressed PR, ERα and GATA3. Conclusion: In presence of lamin A, SBHA downregulated cancer initiators GATA3 and ERα while inducing cell death. These biomarkers could be useful molecular tools prior to initiating targeted breast cancer therapy.","PeriodicalId":22619,"journal":{"name":"The Journal of Cancer Research","volume":"36 1","pages":"60-69"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85309223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

早期胃癌的内镜下诊断和治疗Gastroscopy in the Diagnosis and Treatment of Early Gastric Carcinoma 早期胃癌的内镜下诊断和治疗Gastroscopy in the Diagnosis and Treatment of Early Gastric Carcinoma

The Journal of Cancer Research Pub Date : 2018-06-29 DOI: 10.12677/wjcr.2018.83015

都超群, 袁文照, 张立玮, 赵明巍, 王顺平, 袁宇迪

引用次数: 0

FAK/PI3K/AKt/mTOR信号通路相关蛋白在胃癌中的表达及意义 FAK/PI3K/AKt/mTOR信号通路相关蛋白在胃癌中的表达及意义

The Journal of Cancer Research Pub Date : 2018-06-29 DOI: 10.12677/WJCR.2018.83018

孙玺媛, 于铁夫, 刘洪羽, 曹维海, 樊伟业, 刘晓斌

引用次数: 0

Using a Panel of Heat Shock Proteins for Diagnosis and Prognosis of Breast Cancer 热休克蛋白组在乳腺癌诊断和预后中的应用

The Journal of Cancer Research Pub Date : 2018-06-20 DOI: 10.12691/JCRT-6-2-4

Ebtsam R. Zaher, Mahmoud A. Hemida, Mohammad M. El-Hashash, H. El-Sheridy

{"title":"Using a Panel of Heat Shock Proteins for Diagnosis and Prognosis of Breast Cancer","authors":"Ebtsam R. Zaher, Mahmoud A. Hemida, Mohammad M. El-Hashash, H. El-Sheridy","doi":"10.12691/JCRT-6-2-4","DOIUrl":"https://doi.org/10.12691/JCRT-6-2-4","url":null,"abstract":"Purpose: The current work aimed to evaluate the diagnostic and prognostic role of a panel of heat shock proteins; HSP27, HSP60, HSP70 and HSP90, in sera of breast cancer patients, in comparison with CA 15.3 as the standard marker in breast cancer management. Methodology: The study included 248 females diagnosed with primary breast cancer and 232 normal healthy females. Patients were managed by modified radical mastectomy or breast conservative surgery then received adjuvant therapy and clinically followed up for 5 years. In sera of all patients and controls; HSP27, HSP60, HSP70 and HSP90 were measured by ELISA while CA 15.3 was measured by IRMA. Findings: Pre-treatment serum levels of HSP27, HSP60, HSP70 and HSP90 were significantly elevated in breast cancer patients than in controls, furthermore, they were significant as diagnostic markers, especially when using a panel of HSP70≥9.2 ng/ml and HSP60≥7.5 ng/ml with AUC, sensitivity and specificity of 0.995, 98% and 95%; respectively. As prognostic markers, patients with elevated serum HSP27, HSP60 or HSP70 had significantly lower DFS than patients having lower serum levels. In addition, in patients with at least two HSPs of the three above their cutoffs, the HR increased to 4.65, and it jumped to 17.88 for patients having all three HSPs elevated above their respective cutoff values. CA 15.3 was not significant as diagnostic or prognostic marker. Conclusion: A panel of pre-treatment serum HSP70≥9.2 ng/ml and HSP60≥7.5 ng/ml may be useful for screening of populations at high risk of developing breast cancer. For prediction of treatment response, patients who have elevated pre-treatment serum HSP27, HSP60 or HSP70 had significantly lower DFS. In addition, the risk of relapse in patients having at least two HSPs and patients having all three HSPs elevated above their respective cutoffs were 4.65-times and 17.88-times that of patients with HSPs below their respective cutoffs.","PeriodicalId":22619,"journal":{"name":"The Journal of Cancer Research","volume":"60 1","pages":"47-53"},"PeriodicalIF":0.0,"publicationDate":"2018-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81613211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Post-occlusive Reactive Hyperemia in Mouse Melanoma 小鼠黑色素瘤闭塞后反应性充血

The Journal of Cancer Research Pub Date : 2018-06-05 DOI: 10.17303/JCRTO.2015.3.101

Julien Reyal, N. Lebas

{"title":"Post-occlusive Reactive Hyperemia in Mouse Melanoma","authors":"Julien Reyal, N. Lebas","doi":"10.17303/JCRTO.2015.3.101","DOIUrl":"https://doi.org/10.17303/JCRTO.2015.3.101","url":null,"abstract":"Post-occlusive reactive hyperemia (PORH) is a physiological reaction characterizing the vascular reactivity to application and cessation of blood flow occlusion. Increasing evidence indicates that the PORH may reduce tumor hypoxia and could be used as a clinically relevant method to improve the efficiency of conventional anti-cancer therapies. In order to experiment this new concept, we developed a simple and reproducible mouse model of skin PORH using a leg tourniquet to produce temporary vascular occlusion. Footpad superficial perfusion was continuously measured by laser speckle contrast imaging. We then analyzed the incidence of PORH on B16-F10 melanomas cells injected in the footpad skin. The mouse model reproduced the characteristics of the skin PORH in humans, with a close relationship between the duration of the vascular occlusion and the hyperemia amplitude as well as extent. We also unravelled that PORH also occurred in growing melanoma with a significant 48% median hyperemia after three minutes of vascular occlusion. We therefore described for the first time a PORH phenomenon in a locally very advanced and necrotic murine melanoma that may pave the way for the development of complementary therapeutic approaches to dampen the growth of aggressive tumors.","PeriodicalId":22619,"journal":{"name":"The Journal of Cancer Research","volume":"131 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75285588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Systematic Review and Meta-Analysis of the Accuracy of Confocal Microscopy in the Diagnosis of Skin Cancer 共聚焦显微镜诊断皮肤癌准确性的系统评价和荟萃分析

The Journal of Cancer Research Pub Date : 2018-06-05 DOI: 10.17303/JCRTO.2015.3.104

A. Rahman, S. Miller

{"title":"Systematic Review and Meta-Analysis of the Accuracy of Confocal Microscopy in the Diagnosis of Skin Cancer","authors":"A. Rahman, S. Miller","doi":"10.17303/JCRTO.2015.3.104","DOIUrl":"https://doi.org/10.17303/JCRTO.2015.3.104","url":null,"abstract":"Background: Nonmelanoma skin cancer (Basal Cell Cancer (BCC) and Squamous Cell Cancer (SCC)) are the most prevalent cancer in the light-skinned population. The incidence of melanoma has been increasing steadily throughout the world. Early recognition of skin cancer without doing biopsy remains challenging. The development of noninvasive diagnostic technologies is highly relevant and desired. Confocal Laser Scanning Microscopy (CLSM) enables in vivo and ex vivo imaging of human skin at a quasi-histologic resolution. Methods and Materials: Several databases like Medline, Embase, all EBM reviews (ACP Journal Club, Cochrane Controlled Trials Register, Cochrane Database of Systematic Reviews, Database of Abstracts of Reviews of Effects) and Journals@Ovid were used to perform a literature search on CLSM in the diagnosis of melanoma and non melanoma skin cancers. Standards for Reporting of Diagnosis Accuracy (STARD) initiative checklist and National Institute for Health and Clinical Excellence (NICE) guidelines on methodology were used to assess the quality of the studies found. Eight studies fulfilling relevant criteria inclusive of assessment of diagnostic accuracy of CSLM and histopathology were selected. Heterogeneity among the studies was assessed and the data was pooled for meta- analysis. Results: The eight included studies did not have considerable heterogeneity between them. The pooled sensitivity for melanoma diagnosis was 91.4% and for BCC diagnosis was 90.1%. Pooled specificities were 79.9% and 92.6% for malignant melanoma and BCC respectively. Diagnostic odds ratios for melanoma and BCC were 80.1 and 358.1 respectively. Conclusions: From the limited good quality available literature we found that CLSM has the potential to be an additional noninvasive diagnostic test to dermoscopy for the diagnosis of BCC and melanoma.","PeriodicalId":22619,"journal":{"name":"The Journal of Cancer Research","volume":"93 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83859972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Analysis of Stem-Cell and Migratory Phenotype in Primary Cultures Derived From BPV-Infected Benign and Malignat Neoplasms bpv感染的良恶性肿瘤原代培养物的干细胞和迁移表型分析

The Journal of Cancer Research Pub Date : 2018-06-05 DOI: 10.17303/JCRTO.2017.5.101

R. C. Stocco

引用次数: 4

Induction of Apoptosis in Ehrlich Ascites Carcinoma Cells Through an Intrinsic Pathway by Ni(II)-benzoin Thiosemicarbazone Complex [Ni(BTSC) 2 ] Ni(II)-苯甲酸硫代氨基脲配合物[Ni(BTSC) 2]通过内在途径诱导埃利希腹水癌细胞凋亡

The Journal of Cancer Research Pub Date : 2018-05-29 DOI: 10.12691/jcrt-6-2-3

H. M. Zakir, Md. Nazrul Islam, M. Sarkar, Amit Kumar Dey, Ruhul Amin, Jahanara Khanam, M. Jesmin, H. Nur, S. M. M. Ali

{"title":"Induction of Apoptosis in Ehrlich Ascites Carcinoma Cells Through an Intrinsic Pathway by Ni(II)-benzoin Thiosemicarbazone Complex [Ni(BTSC) 2 ]","authors":"H. M. Zakir, Md. Nazrul Islam, M. Sarkar, Amit Kumar Dey, Ruhul Amin, Jahanara Khanam, M. Jesmin, H. Nur, S. M. M. Ali","doi":"10.12691/jcrt-6-2-3","DOIUrl":"https://doi.org/10.12691/jcrt-6-2-3","url":null,"abstract":"Cancer is one of the leading causes of morbidity and mortality through worldwide. Globally cancer recognized as the second leading cause of death. Therefore, the discovery and development of new potent and selective anticancer drugs are of high importance in modern cancer research. The objective of this study was to find out the mechanism through which Ni(II)-benzoin thiosemicarbazone complex exerts its antitumor activity against Ehrlich Ascites Carcinoma (EAC) cells bearing swiss albino mice. Induction of apoptosis in EAC cells was confirmed by observation of nuclear morphology and DNA fragmentation assay. The mRNA expression of several apoptotic genes like B-cell lymphoma 2 (bcl-2), B-cell lymphoma extra-large (bcl-xL) caspase-8, and proapoptotic genes p53 or tumor protein, bcl-2 associated X protein (bax), caspase-9, caspase-3 and poly-ADP ribose polymerase (PARP-1) reveal the induction of apoptosis by Ni(BTSC)2 in EAC cell. Inhibition of Ni(BTSC)2 induced apoptosis by Caspase 3 inhibitor treatment affirmed that the induction of intrinsic apoptosis pathway on EAC cells. Reactive Oxygen Species (ROS) generation after Ni(BTSC)2 treatment confirmed that the induction of apoptosis by Ni(BTSC)2 occurred through an ROS-dependent mitochondria-mediated intrinsic pathway rather than an extrinsic pathway. Thus, this study provides evidence to carry out further researches in a way to formulate novel anticancer drugs.","PeriodicalId":22619,"journal":{"name":"The Journal of Cancer Research","volume":"156 1","pages":"39-46"},"PeriodicalIF":0.0,"publicationDate":"2018-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78647773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1