{"title":"小鼠黑色素瘤闭塞后反应性充血","authors":"Julien Reyal, N. Lebas","doi":"10.17303/JCRTO.2015.3.101","DOIUrl":null,"url":null,"abstract":"Post-occlusive reactive hyperemia (PORH) is a physiological reaction characterizing the vascular reactivity to application and cessation of blood flow occlusion. Increasing evidence indicates that the PORH may reduce tumor hypoxia and could be used as a clinically relevant method to improve the efficiency of conventional anti-cancer therapies. In order to experiment this new concept, we developed a simple and reproducible mouse model of skin PORH using a leg tourniquet to produce temporary vascular occlusion. Footpad superficial perfusion was continuously measured by laser speckle contrast imaging. We then analyzed the incidence of PORH on B16-F10 melanomas cells injected in the footpad skin. The mouse model reproduced the characteristics of the skin PORH in humans, with a close relationship between the duration of the vascular occlusion and the hyperemia amplitude as well as extent. We also unravelled that PORH also occurred in growing melanoma with a significant 48% median hyperemia after three minutes of vascular occlusion. We therefore described for the first time a PORH phenomenon in a locally very advanced and necrotic murine melanoma that may pave the way for the development of complementary therapeutic approaches to dampen the growth of aggressive tumors.","PeriodicalId":22619,"journal":{"name":"The Journal of Cancer Research","volume":"131 1","pages":"1-5"},"PeriodicalIF":0.0000,"publicationDate":"2018-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Post-occlusive Reactive Hyperemia in Mouse Melanoma\",\"authors\":\"Julien Reyal, N. Lebas\",\"doi\":\"10.17303/JCRTO.2015.3.101\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Post-occlusive reactive hyperemia (PORH) is a physiological reaction characterizing the vascular reactivity to application and cessation of blood flow occlusion. Increasing evidence indicates that the PORH may reduce tumor hypoxia and could be used as a clinically relevant method to improve the efficiency of conventional anti-cancer therapies. In order to experiment this new concept, we developed a simple and reproducible mouse model of skin PORH using a leg tourniquet to produce temporary vascular occlusion. Footpad superficial perfusion was continuously measured by laser speckle contrast imaging. We then analyzed the incidence of PORH on B16-F10 melanomas cells injected in the footpad skin. The mouse model reproduced the characteristics of the skin PORH in humans, with a close relationship between the duration of the vascular occlusion and the hyperemia amplitude as well as extent. We also unravelled that PORH also occurred in growing melanoma with a significant 48% median hyperemia after three minutes of vascular occlusion. We therefore described for the first time a PORH phenomenon in a locally very advanced and necrotic murine melanoma that may pave the way for the development of complementary therapeutic approaches to dampen the growth of aggressive tumors.\",\"PeriodicalId\":22619,\"journal\":{\"name\":\"The Journal of Cancer Research\",\"volume\":\"131 1\",\"pages\":\"1-5\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-06-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Journal of Cancer Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17303/JCRTO.2015.3.101\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Cancer Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17303/JCRTO.2015.3.101","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Post-occlusive Reactive Hyperemia in Mouse Melanoma
Post-occlusive reactive hyperemia (PORH) is a physiological reaction characterizing the vascular reactivity to application and cessation of blood flow occlusion. Increasing evidence indicates that the PORH may reduce tumor hypoxia and could be used as a clinically relevant method to improve the efficiency of conventional anti-cancer therapies. In order to experiment this new concept, we developed a simple and reproducible mouse model of skin PORH using a leg tourniquet to produce temporary vascular occlusion. Footpad superficial perfusion was continuously measured by laser speckle contrast imaging. We then analyzed the incidence of PORH on B16-F10 melanomas cells injected in the footpad skin. The mouse model reproduced the characteristics of the skin PORH in humans, with a close relationship between the duration of the vascular occlusion and the hyperemia amplitude as well as extent. We also unravelled that PORH also occurred in growing melanoma with a significant 48% median hyperemia after three minutes of vascular occlusion. We therefore described for the first time a PORH phenomenon in a locally very advanced and necrotic murine melanoma that may pave the way for the development of complementary therapeutic approaches to dampen the growth of aggressive tumors.