The Journal of Biophysical and Biochemical Cytology最新文献

筛选
英文 中文
Fine structure of the photosynthetic bacterium Rhodomicrobium vannielii. 光合细菌菱形菌的精细结构。
The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.469
E S BOATMAN, H C DOUGLAS
{"title":"Fine structure of the photosynthetic bacterium Rhodomicrobium vannielii.","authors":"E S BOATMAN,&nbsp;H C DOUGLAS","doi":"10.1083/jcb.11.2.469","DOIUrl":"https://doi.org/10.1083/jcb.11.2.469","url":null,"abstract":"<p><p>The fine structure of the photosynthetic bacterium Rhodomicrobium vannielii was studied by the ultra thin sectioning technique. Cells were fixed in buffered osmium tetroxide and embedded in Epoxy resin. The feature most common to nearly all cells was an array of intracellular membranes situated in a concentric manner at the periphery of the cell. The membranes were mostly paired and quite often five pairs were seen aligned together. Calculations from densitometric tracings showed the average width of a \"unit\" membrane to be 65 A. Sections of material from disrupted cells after passage through a sucrose gradient revealed vesicular forms composed of membranes similar in width to those in the intact cell. Absorption spectra of both intact cells and isolated membranes were very similar in the bacteriochlorophyll regions. Septa and membranes were demonstrated in the filaments that join mature cells. No evidence for chromatophores was obtained although the methods used were adequate for their demonstration in Rhodospirillum rubrum.</p>","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"469-83"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.2.469","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23462279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 50
Some theoretical aspects of osmium tetroxide fixation with special reference to the metaphase chromosomes of cell cultures. 四氧化锇固定的一些理论方面,特别涉及细胞培养的中期染色体。
The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.449
E ROBBINS
{"title":"Some theoretical aspects of osmium tetroxide fixation with special reference to the metaphase chromosomes of cell cultures.","authors":"E ROBBINS","doi":"10.1083/jcb.11.2.449","DOIUrl":"https://doi.org/10.1083/jcb.11.2.449","url":null,"abstract":"<p><p>Fixation of cell cultures with 1 per cent OsO(4) at constant pH and tonicity but variable cationic valence and dielectric constant causes profound changes in metaphase chromosomes. It is possible to make them disappear, flocculate, or show little change from the living cell in the phase contrast microscope. Conventional fixation for the electron microscope causes almost complete disappearance of metaphase chromosomes in phase contrast. Reasons for this behavior are discussed. It is postulated that intermolecular distances and consequently internal structure in chromosomes are governed by the same forces that govern these distances in colloidal sols.</p>","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":" ","pages":"449-55"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40811668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Transient phosphorylation of deoxyribosides and regulation of deoxyribonucleic acid synthesis. 脱氧核糖苷的瞬时磷酸化和脱氧核糖核酸合成的调控。
The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.311
Y HOTTA, H STERN
{"title":"Transient phosphorylation of deoxyribosides and regulation of deoxyribonucleic acid synthesis.","authors":"Y HOTTA,&nbsp;H STERN","doi":"10.1083/jcb.11.2.311","DOIUrl":"https://doi.org/10.1083/jcb.11.2.311","url":null,"abstract":"<p><p>Microspores isolated from Lilium longiflorum and Trillium erectum were studied with respect to their capacities for phosphorylating deoxyribosides in vitro. It was found that such capacities are manifest only during brief intervals of time adjacent to periods of DNA synthesis, and that none of the neighboring cells in the anther acquire them. The observed patterns of behavior are interpreted in terms of enzyme induction as a device for regulating DNA synthesis.</p>","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"311-9"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.2.311","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23970132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 49
Localization of ATPase in rotifer cilia. 轮虫纤毛中atp酶的定位。
The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.498
A I LANSING, F LAMY
{"title":"Localization of ATPase in rotifer cilia.","authors":"A I LANSING,&nbsp;F LAMY","doi":"10.1083/jcb.11.2.498","DOIUrl":"https://doi.org/10.1083/jcb.11.2.498","url":null,"abstract":"","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"498-501"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.2.498","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23981216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 30
Staining methods applicable to sections of osmium-fixed tissue for light microscopy. 适用于光显微镜下锇固定组织切片的染色方法。
The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.502
B L MUNGER
{"title":"Staining methods applicable to sections of osmium-fixed tissue for light microscopy.","authors":"B L MUNGER","doi":"10.1083/jcb.11.2.502","DOIUrl":"https://doi.org/10.1083/jcb.11.2.502","url":null,"abstract":"","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"502-6"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.2.502","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23995725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 101
Electron microscopy of DNA molecules "stained" with heavy metal salts. 被重金属盐“染色”的DNA分子的电子显微镜。
The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.297
W STOECKENIUS
{"title":"Electron microscopy of DNA molecules \"stained\" with heavy metal salts.","authors":"W STOECKENIUS","doi":"10.1083/jcb.11.2.297","DOIUrl":"https://doi.org/10.1083/jcb.11.2.297","url":null,"abstract":"<p><p>Single DNA molecules can be rendered visible in the electron microscope by \"staining\" with water-soluble salts of heavy metals. The best results were obtained with lanthanum nitrate, uranyl acetate, and lead perchlorate. The molecules appear as filaments approximately 20 A wide. Their length was not determined, but it could be shown that it varied with the molecular weight of the DNA used. The same heavy metal salts will preferentially \"stain\" the nucleic acid in a protein-DNA complex. Evidence is provided for the possibility of a partial separation of a double-stranded molecule into single strands on adsorption to the supporting film.</p>","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"297-310"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.2.297","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23508464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 71
THE ENDOPLASMIC RETICULUM OF GASTRIC PARIETAL CELLS. 胃壁细胞的内质网。
The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.333
S Ito
{"title":"THE ENDOPLASMIC RETICULUM OF GASTRIC PARIETAL CELLS.","authors":"S Ito","doi":"10.1083/jcb.11.2.333","DOIUrl":"https://doi.org/10.1083/jcb.11.2.333","url":null,"abstract":"<p><p>An electron microscopic survey has been made of the gastric parietal or oxyntic cell of the human, cat, beaver, dog, hamster, rat, mouse, and bat, and of the corresponding cell type in two species of frog, two species of toad, and the horned lizard. A feature consistently found in the parietal cells of the mammals or their equivalent in the lower vertebrates is the agranular endoplasmic reticulum, which takes the form of branching and anastomosing small tubules approximately 200 to 500 A in diameter, sometimes expanded into flattened cisternae. In mammalian parietal cells this form of the endoplasmic reticulum is found only in limited amounts, but in the corresponding secretory cells of the amphibia and reptilia the tubular agranular reticulum is abundant. It is believed to comprise a more or less continuous system of channels, but owing to their tortuous course only short profiles are seen in thin sections. Immediately subjacent to the plasmalemma at the free surface, the cytoplasm is relatively free of organelles but is occasionally traversed by the agranular reticulum, which appears to be continuous at some points with the cell surface. The possible participation of the agranular endoplasmic reticulum in hydrochloric acid secretion is discussed.</p>","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 2","pages":"333-47"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.2.333","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28466710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 135
Serological studies on the formation of protein parasporal inclusions in Bacillus thuringiensis. 苏云金芽孢杆菌蛋白副孢包涵体形成的血清学研究。
The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.321
R E MONRO
{"title":"Serological studies on the formation of protein parasporal inclusions in Bacillus thuringiensis.","authors":"R E MONRO","doi":"10.1083/jcb.11.2.321","DOIUrl":"https://doi.org/10.1083/jcb.11.2.321","url":null,"abstract":"<p><p>A strain of Bacillus thuringiensis has been isolated, and methods have been developed for separation of the crystalline, parasporal inclusions in a pure form. Normal sporulation with concomitant crystal formation takes place when cells are incubated under suitable conditions in a nutrient free medium. Serological techniques have been used to study the origin and development of the crystals. Rabbit antisera have been prepared to a vegetative cell extract, suspensions of crystals, and a solution of crystal protein (obtained by alkali treatment of crystals). Tests have been carried out mainly by the Ouchterlony gel plate technique. Crystal protein solutions were found to be more active than suspensions of intact crystals both in reaction with, and in neutralization of, the crystal antibodies. Antisera to the vegetative cell extract gave no reaction with crystal protein. Ultrasonic extracts of cells taken before or during crystal formation gave no reaction with the crystal antibodies. Tests with alkali extracts of disrupted cells showed that the crystal antigen is absent in vegetative cells but arises during sporulation. The appearance of the antigen can be correlated with the formation and growth of the crystals as followed by examination of disrupted cell preparations under the electron microscope. It can be concluded that the crystalline protein inclusions do not arise from precursors in the same antigenic state.</p>","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"321-31"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.2.321","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23995143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
Historadiographic identification of alkaline phosphatase. 碱性磷酸酶的放射学鉴定。
The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.488
A J HALE
{"title":"Historadiographic identification of alkaline phosphatase.","authors":"A J HALE","doi":"10.1083/jcb.11.2.488","DOIUrl":"https://doi.org/10.1083/jcb.11.2.488","url":null,"abstract":"","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"488-92"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.2.488","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23492830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Light and electron microscope studies of mycobacterium--mycobacteriophage interactions. III. Further studies on the ultrathin sections. 分枝杆菌-噬菌体相互作用的光学和电子显微镜研究。3超薄切片的进一步研究。
The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.441
K TAKEYA, M KOIKE, R MORI, T TODA
{"title":"Light and electron microscope studies of mycobacterium--mycobacteriophage interactions. III. Further studies on the ultrathin sections.","authors":"K TAKEYA,&nbsp;M KOIKE,&nbsp;R MORI,&nbsp;T TODA","doi":"10.1083/jcb.11.2.441","DOIUrl":"https://doi.org/10.1083/jcb.11.2.441","url":null,"abstract":"The process of multiplication of mycobacteriophage B-1 in its host cell was studied by means of an improved technic of ultrathin sectioning. The appearance of the nuclear apparatus was not altered throughout the latent period. Phage-shaped dense particles appeared about 30 minutes after infection in less dense areas neighboring the nuclear apparatus and occasionally at the margin of the nuclear apparatus. The less dense areas, which may correspond to the phage multiplication foci according to the authors' interpretation, were not filled with such arrays of fine-stranded fibrils as are seen in the nuclear apparatus. Empty phage heads could frequently be seen within and outside the lysed cells, along with the mature phage particles, at the end of the latent period. Moreover, it was indicated that empty head membranes may possibly exist within the cells during the latent period","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"441-7"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.2.441","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23507288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信