The Journal of Biophysical and Biochemical Cytology最新文献_第2页

A small granular component of the cytoplasm of keratinizing epithelia. 角化上皮细胞质中的小颗粒成分。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-12-01 DOI: 10.1083/jcb.11.3.719

J V FREI, H SHELDON

引用次数: 59

The two-wavelength method of microspectrophotometry. IV. A new solution. 双波长显微分光光度法。四、新的解决方案。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-12-01 DOI: 10.1083/jcb.11.3.509

M L MENDELSOHN

引用次数: 16

Electron microscopic and histochemical comparison of the two types of electroplaques of Narcine brasiliensis. 巴西水仙两种电斑的电镜及组织化学比较。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-12-01 DOI: 10.1083/jcb.11.3.663

A WACHTEL, R MATHEWSON, H GRUNDFEST

{"title":"Electron microscopic and histochemical comparison of the two types of electroplaques of Narcine brasiliensis.","authors":"A WACHTEL, R MATHEWSON, H GRUNDFEST","doi":"10.1083/jcb.11.3.663","DOIUrl":"https://doi.org/10.1083/jcb.11.3.663","url":null,"abstract":"The torpedine electric fish Narcine brasiliensis has two morphologically distinct electric organs (main and accessory) which also differ with respect to a number of electrophysiological properties. The fine structure of the electroplaques of these organs has been examined by electron microscopy and by a histochemical method for localizing esterase activity with a high degree of resolution. In both kinds of electroplaques the innervated surface (ventral in those of the main organ, dorsal in those of the accessory) is the only site of esterase activity. The latter is further confined to the regions of synaptic contact between vesicle-containing axon terminals and the electroplaque membrane. The synaptic apparatus is similar to, but less elaborate than, that of neuromuscular junctions. The axon terminals and electroplaque membranes are free of connective tissue envelopments. The membrane of the uninnervated surfaces forms a continuum with a dense canalicular network which penetrates deeply into the 7 micro thick electroplaques of the main organ. The canalicular network has about the same thickness in the 20 micro electroplaques of the accessory organ. Except for this difference, the two kinds of cells appear to have the same fine structure. This finding is discussed in relation to the electrophysiological data on functional differences.","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"663-75"},"PeriodicalIF":0.0,"publicationDate":"1961-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.3.663","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23623871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Histochemistry of the centrioles and centrosomes of the leukemic cells from human myeloblastic leukemia. 人成髓细胞白血病白血病细胞中心粒和中心体的组织化学研究。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-12-01 DOI: 10.1083/jcb.11.3.717

G A ACKERMAN

引用次数: 0

On the fine structure and composition of the nuclear envelope. 论核膜的精细结构和组成。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-12-01 DOI: 10.1083/jcb.11.3.559

R W MERRIAM

{"title":"On the fine structure and composition of the nuclear envelope.","authors":"R W MERRIAM","doi":"10.1083/jcb.11.3.559","DOIUrl":"https://doi.org/10.1083/jcb.11.3.559","url":null,"abstract":"Nuclei from nearly ripe eggs of Rana pipiens were isolated and cleaned in 0.1 M KCl. The whole nucleus was then digested to various degrees with ribonuclease or trypsin, followed by washing and fixation in either osmium tetroxide or potassium permanganate. The nuclear envelope was dissected off, placed on a grid, air dried, and compared with undigested controls in the electron microscope. Some envelopes were dehydrated, embedded in methacrylate, and sectioned. Annuli around \"pores\" are composed of a substance or substances, at least partially fibrillar, which is preserved by osmium but lost during permanganate fixation. Material within the \"pores\" is also preserved by osmium but partially lost after permanganate. No evidence of granules or tubules in the annuli was found in air dried mounts although a granular appearance could be seen in tangentially oriented thin sections. Thin sections of isolated envelopes give evidence of diffuse material within the \"pores\" as well as a more condensed diaphragm across their waists. In whole mounts of the envelope the total density within \"pores\" is relatively constant from \"pore\" to \"pore.\" All material within \"pores,\" including the condensed diaphragm, is removable by trypsin digestion. Wispy material from the \"pore\" structure projects into the nucleus and annular material extends into the cytoplasm. Both annular and diaphragm materials remain with the envelope when it is isolated and are thus considered a part of its structure, not merely evidences of material passing through. There is no evidence of ribonuclease-removable material in any part of the \"pore\" complex.","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"559-70"},"PeriodicalIF":0.0,"publicationDate":"1961-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.3.559","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23991820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 75

Corpus intra cristam: a dense body within mitochondria of cells in hyperplastic mouse epidermis. 嵴内体:增生小鼠表皮细胞线粒体内的致密体。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-12-01 DOI: 10.1083/jcb.11.3.724

J V FREI, H SHELDON

引用次数: 50

Simple methods for "staining with lead" at high pH in electron microscopy. 在电子显微镜下用高pH值“铅染色”的简单方法。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-12-01 DOI: 10.1083/jcb.11.3.729

M J KARNOVSKY

引用次数: 1377

A radial component of central myelin sheaths. 髓鞘中央髓鞘的放射状组成部分。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-12-01 DOI: 10.1083/jcb.11.3.733

A PETERS

引用次数: 69

The use of specific antibody in electron microscopy. II. The visualization of mercury-labeled antibody in the electron microscope. 电子显微镜下特异性抗体的使用。2汞标记抗体在电子显微镜下的可视化。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-12-01 DOI: 10.1083/jcb.11.3.521

F A PEPE, H FINCK

引用次数: 41

CORRELATION OF THE FINE STRUCTURE OF THE GASTRIC PARIETAL CELL (DOG) WITH FUNCTIONAL ACTIVITY OF THE STOMACH. 犬胃壁细胞精细结构与胃功能活动的关系。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.349

A W Sedar, M H Friedman

{"title":"CORRELATION OF THE FINE STRUCTURE OF THE GASTRIC PARIETAL CELL (DOG) WITH FUNCTIONAL ACTIVITY OF THE STOMACH.","authors":"A W Sedar, M H Friedman","doi":"10.1083/jcb.11.2.349","DOIUrl":"https://doi.org/10.1083/jcb.11.2.349","url":null,"abstract":"The fine structure of the parietal (oxyntic) cell in the gastric glands (corpus of the stomach) of the dog was examined under conditions of active gastric acid secretion and compared with cellular structure in the non-acid-secretory (basal) state. Animals, in both acute and chronic experiments, were equipped with gastric fistulae so that gastric juice could be collected for analysis of total acidity, free acidity, volume, and pH prior to biopsy of the gastric mucosa. The specimens of mucosa were fixed in buffered OsO(4) and embedded in n-butyl methacrylate and the thin sections were stained with lead hydroxide before examination in the electron microscope. A majority of parietal cells showed an alteration of fine structure during stimulation of gastric acid secretion by a number of different techniques (electrical vagal stimulation, histamine administration, or insulin injection). The changes in fine structure affected mainly the smooth surfaced vesicular elements and the intracellular canaliculi in the cytoplasm of the cell. The mitochondria also appeared to be involved to some extent. During acid secretion a greater concentration of smooth surface profiles is found adjacent to the walls of the intracellular canaliculi; other parietal cells exhibited a marked decrease in number of smooth surfaced elements. Intracellular canaliculi, always present in non-acid-secreting oxyntic cells, develop more extensively in cells of acid-secreting gastric glands. The surface area of these canaliculi is greatly increased by the elaboration of a large number of closely approximated and elongated microvilli. Still other parietal cells apparently in a different stage of the secretory cycle exhibit non-patent canaliculi lacking prominence; such cells have very few smooth surfaced vesicular elements. These morphological findings correlated with the acid-secretory state of the stomach provide evidence that the parietal cell participates in the process of acid secretion.","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 2","pages":"349-63"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.2.349","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28466711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 105