The Journal of Biophysical and Biochemical Cytology最新文献_第4页

Fine structure and pigment conversion in isolated etiolated proplastids. 黄化前质体的精细结构和色素转化。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.433

S KLEIN, A POLJAKOFF-MAYBER

引用次数: 22

Electron microscopic study of the cytopathology of ECHO virus infection in cultivated cells. 培养细胞感染ECHO病毒的细胞病理学电镜研究。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.457

O NUNEZ-MONTIEL, J WEIBEL, J VITELLI-FLORES

{"title":"Electron microscopic study of the cytopathology of ECHO virus infection in cultivated cells.","authors":"O NUNEZ-MONTIEL, J WEIBEL, J VITELLI-FLORES","doi":"10.1083/jcb.11.2.457","DOIUrl":"https://doi.org/10.1083/jcb.11.2.457","url":null,"abstract":"The cultivated monkey kidney cell is subject to changes when infected with ECHO viruses 6, 9, and 19. The electron microscope reveals three stages of infection: (a) initial stage. The nucleus appears granular with chromatin condensation on the nuclear envelope. The cytoplasm contains electron transparent vesicles and vacuoles forming nests. (b) Intermediate stage. The nucleus seems to diminish, appearing more pycnotic and displaced toward the periphery. The cytoplasm is filled with electron transparent vacuoles and vesicles, and dense masses as well as some spiral bodies are seen. The mitochondria retain their shape. Dense particles are seen, which are possibly of viral nature. (c) Final stage. The nucleus is contracted to a narrow strip close to the cellular membrane or is completely destroyed. The cytoplasm shows no apparent changes. Crystals are frequently observed in cells infected with ECHO viruses 6 and 19, consisting of dense particles with an average diameter of 14.4 mmicro ranging from approximately 13.2 to 15.6 mmicro for ECHO virus 6, and 14.5 mmicro ranging from approximately 12.5 to 16.5 mmicro for ECHO virus 19. These particles are clustered in hexagonal packages forming angles of 75 degrees and 105 degrees . The particles in most crystals are arranged in rows separated by a constant distance, the latter varying from one crystal to another and being approximately 1.5 and 2.5 times the distance between particles. Other particles were observed which, however, are not considered to be of viral nature.","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"457-67"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.2.457","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23999364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Preferential staining of nucleic acid-containing structures for electron microscopy. 电子显微镜对含核酸结构的优先染色。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.273

H E HUXLEY, G ZUBAY

{"title":"Preferential staining of nucleic acid-containing structures for electron microscopy.","authors":"H E HUXLEY, G ZUBAY","doi":"10.1083/jcb.11.2.273","DOIUrl":"https://doi.org/10.1083/jcb.11.2.273","url":null,"abstract":"Oriented fibres of extracted nucleohistone were employed as test material in a study of satisfactory fixation, embedding, and staining methods for structures containing a high proportion of nucleic acid. Fixation in buffered osmium tetroxide solution at pH 6, containing 10(-2)M Ca(++), and embedding in Araldite enabled sections of the fibres to be cut in which the orientation was well preserved. These could be strongly stained in 2 per cent aqueous uranyl acetate, and showed considerable fine structure. Certain regions in the nuclei of whole thymus tissue could also be strongly stained by the same procedure, and were identical with the regions stained by the Feulgen procedure in adjacent sections. Moreover, purified DNA was found to take up almost its own dry weight of uranyl acetate from 2 per cent aqueous solution. Strongest staining of whole tissue was obtained with very short fixation times-5 minutes or so at 0 degrees C. Particularly intense staining was obtained when such tissue stained in uranyl acetate was further stained with lead hydroxide. Although the patterns of staining by lead hydroxide alone and by uranyl acetate were similar in tissues fixed for longer times ((1/2) hour to 2 hours, at 0 degrees C or 20 degrees C), in briefly fixed material the DNA-containing regions appeared relatively unstained by lead hydroxide alone, whilst often there was appreciable staining of RNA-containing structures. Observations on the staining of some viruses by similar techniques are also described.","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"273-96"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.2.273","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23969880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 372

The ultrastructure and histophysiology of human eccrine sweat glands. 人汗腺的超微结构及组织生理。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.385

B L MUNGER

引用次数: 86

Electron microscope study of mitosis in sea urchin blastomeres. 海胆卵裂球有丝分裂的电镜研究。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.419

P HARRIS

{"title":"Electron microscope study of mitosis in sea urchin blastomeres.","authors":"P HARRIS","doi":"10.1083/jcb.11.2.419","DOIUrl":"https://doi.org/10.1083/jcb.11.2.419","url":null,"abstract":"The fine structure of cells at different stages of the mitotic cycle was studied in the blastomeres of 6-hour-old embryos of the sea urchin Strongylocentrotus purpuratus. The material was fixed in 1 per cent osmium tetroxide in sea water, buffered with veronal-acetate to pH 7.5, embedded in Araldite, and sectioned with glass knives. The aster, as it forms around the centriole, has the appearance of the endoplastic reticulum, with elements oriented radially from the centrosphere to the periphery of the cell. Anaphase structures described include the kinetochores, with bundles of fine filaments extending toward the centrioles, as well as continuous filaments passing between the chromosomes. Two cylindrical centrioles composed of parallel rods are present in each of the anaphase asters. At late anaphase, elements of the endoplasmic reticulum condense on the surface of the chromosomes to form a double membrane which already at this stage possesses pores or annuli. At telophase bundles of continuous filaments can be seen in the interzonal region. These filaments, as well as those associated with the chromosomes, have a diameter of approximately 15 mmicro, and appear physically different from the astral structure.","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"419-31"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.2.419","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23493277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 96

An electron microscopic study of eccrine sweat glands of the catfoot and toe pads--evidence for ductal reabsorption in the human. 猫足和趾垫分泌汗腺的电子显微镜研究——人类导管重吸收的证据。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.403

B L MUNGER, S W BRUSILOW

引用次数: 55

Asynchronous replication of HeLa S3 chromosomal deoxyribonucleic acid. HeLa S3染色体脱氧核糖核酸的异步复制。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.485

R B PAINTER

引用次数: 27

Methods for the use of indium as an electron stain for nucleic acids. 用铟作核酸电子染色剂的方法。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.257

M L WATSON, W G ALDRIDGE

引用次数: 86

A simple method for obtaining increased contrast in araldite sections by using postfixation staining of tissues with potassium permanganate. 利用高锰酸钾对组织进行固定后染色，一种简单的方法来提高盐盐切片的对比度。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.492

D F PARSONS

引用次数: 70

Maturation of the rat fetal thyroid. 大鼠胎儿甲状腺发育成熟。

The Journal of Biophysical and Biochemical Cytology Pub Date : 1961-11-01 DOI: 10.1083/jcb.11.2.365

J D FELDMAN, J J VAZQUEZ, S M KURTZ

{"title":"Maturation of the rat fetal thyroid.","authors":"J D FELDMAN, J J VAZQUEZ, S M KURTZ","doi":"10.1083/jcb.11.2.365","DOIUrl":"https://doi.org/10.1083/jcb.11.2.365","url":null,"abstract":"Maturation of the rat fetal thyroid was studied with the aid of I(131) and of fluorescence and electron microscopy. The I(131) concentration of the fetal gland increased exponentially from day 17 to day 20 of gestation and was related to the weight of the fetus (and presumably the weight of the thyroid) and also to the quantity of I(131) accumulated by the fetus. In the 17-day gland, thyroglobulin or immunologically similar material was sparsely present in the incipient lumens of some cell clusters. With maturation, this material increased and was also observed within follicular cells on days 18 to 19 of gestation. On day 20, the specifically reacting material was present in the follicular lumens and was absent from the cytoplasm of follicular epithelium. Ultrastructurally, the earliest thyroid cells examined were replete with all the organelles found in the more mature epithelium. No direct correlation could be made between the cytoplasmic structures and the presence of thyroglobulin, although the granular endoplasmic reticulum was most likely the organelle responsible for synthesis of thyroglobulin. Thyroglobulin or a precursor was found in fetal thyroid cells before measurable quantities of I(131) were concentrated and before cytoplasmic droplets appeared.","PeriodicalId":22618,"journal":{"name":"The Journal of Biophysical and Biochemical Cytology","volume":"11 ","pages":"365-83"},"PeriodicalIF":0.0,"publicationDate":"1961-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1083/jcb.11.2.365","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23480906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 53