The EuroBiotech Journal最新文献_第6页

Comparison of three diagnostic methods in the diagnosis of cryptosporidiosis and gp60 subtyping of Cryptosporidium parvum in diarrheic calves in Central Anatolia Region of Turkey 三种诊断方法对土耳其中部安纳托利亚地区腹泻小牛隐孢子虫病的诊断及gp60亚型的比较

IF 1.7

The EuroBiotech Journal Pub Date : 2021-04-01 DOI: 10.2478/ebtj-2021-0010

A. Yıldırım, F. Sevinc, Z. Onder, O. Duzlu, O. Ekici, N. Işik, A. Çiloğlu, E. Şimşek, Gamze Yetişmiş, A. Inci

{"title":"Comparison of three diagnostic methods in the diagnosis of cryptosporidiosis and gp60 subtyping of Cryptosporidium parvum in diarrheic calves in Central Anatolia Region of Turkey","authors":"A. Yıldırım, F. Sevinc, Z. Onder, O. Duzlu, O. Ekici, N. Işik, A. Çiloğlu, E. Şimşek, Gamze Yetişmiş, A. Inci","doi":"10.2478/ebtj-2021-0010","DOIUrl":"https://doi.org/10.2478/ebtj-2021-0010","url":null,"abstract":"Abstract The aim of this study was to compare three diagnostic methods for the diagnosis of cryptosporidiosis and to detect subtypes ofCryptosporidium parvum by sequences analyses of gp60 gene in diarrheic calves in several herds in Konya province located in Central Anatolia Region of Turkey. Fecal samples were collected from a total of 194 pre-weaned calves (n=158, ≤15 days old, and n=36, 15 to 40 days old), with diarrhoea. For comparative diagnosis, all samples were examined by modified Ziehl-Neelsen staining of fecal smears for the presence of oocyst, nested PCR-RFLP of SSU rRNA and TaqMan qPCR for the detection of Cryptosporidium DNA. A total of 92 (47.4%) and 104 (53.6%) out of the examined samples were found positive by microscopic examination and molecular tools, respectively. The diagnostic sensitivity and specificity of microscopic identification were determined as 88.5% and 100.0%, respectively compared to molecular assays. Cryptosporidium parvum was the only detected species in all positive samples by species-specific qPCR and nested PCR-RFLP assays. Species identifications were further confirmed by sequence analyses of the SSU rRNA PCR products. There was no statistically significant difference in C. parvum prevalence between early pre-weaned calves and calves older than 15 days. The sequence analyses of the gp60 gene of C. parvum isolates revealed a one subtype IIaA13G2R1 belonging to zoonotic family IIa in diarrheic calves","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"5 1","pages":"63 - 69"},"PeriodicalIF":1.7,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69198774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Citric Acid Production of Yeasts: An Overview 酵母柠檬酸生产综述

IF 1.7

The EuroBiotech Journal Pub Date : 2021-04-01 DOI: 10.2478/ebtj-2021-0012

Bilge Sayın Börekçi, G. Kaban, M. Kaya

{"title":"Citric Acid Production of Yeasts: An Overview","authors":"Bilge Sayın Börekçi, G. Kaban, M. Kaya","doi":"10.2478/ebtj-2021-0012","DOIUrl":"https://doi.org/10.2478/ebtj-2021-0012","url":null,"abstract":"Abstract Background Citric acid, an intermediate product of the Krebs cycle, has a wide usage area in the food industry since it has some functions such as acidulant, flavouring agent, preservative and antioxidant. Although molds are the most commonly used microorganisms in the citric acid production, it is known that there are significant advantages of using yeasts. Purpose and scope The microbial citric acid production mechanism needs to be well understood to make production more efficient. In this study, the yeasts used in the production, fermentation types and the factors affecting production were reviewed with studies. Methodology Although production of citric acid can be produced by chemical synthesis, the fermentation is preferred because of its low cost and ease of use. More than 90% of citric acid produced in the world is obtained by fermentation. Results Yarrowia lipolytica, Candida zeylanoides and Candida oleophila are evaluated for citric acid production with substrates such as molasses, glucose, sucrose and glycerol. On the other hand, there is great interest in developing processes with new substrates and/ or microorganisms. Conclusion Although the microbial strain is an important factor, the factors such as carbon, phosphorus and nitrogen sources, aeration, the presence of trace elements and pH are also parameters affecting the production.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"5 1","pages":"79 - 91"},"PeriodicalIF":1.7,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48336077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Detection of Biological Activity of Curcuma longa Methanol Extract and Its Antibacterial effect on Staphylococcus aureus 姜黄甲醇提取物的生物活性检测及其对金黄色葡萄球菌的抗菌作用

IF 1.7

The EuroBiotech Journal Pub Date : 2021-04-01 DOI: 10.2478/ebtj-2021-0009

C. Ogbonna, Erkay Özgör

{"title":"Detection of Biological Activity of Curcuma longa Methanol Extract and Its Antibacterial effect on Staphylococcus aureus","authors":"C. Ogbonna, Erkay Özgör","doi":"10.2478/ebtj-2021-0009","DOIUrl":"https://doi.org/10.2478/ebtj-2021-0009","url":null,"abstract":"Abstract Plants are known to possess enormous profound benefits, which if well incorporated in daily living have the potential to influence our health in ways unexpected. Significant progress has been made concerning the anti-oxidative and anti-cancerous effect of bioactive components in food products, worthy of note in the curcuminoids derived from turmeric (Curcuma longa) rhizomes. Therefore, to this end, this research aims to describe succinctly the phytocomponents, antioxidant activity and anti-microbial activity of turmeric methanol extract. The zones of inhibition were measured after plating on a Mueller Hinton agar plate and compared to the standards penicillin G and chloramphenicol. Chloramphenicol used as a positive control was found to be most effective on Staphylococcus aureus while Curcuma longa methanol extract and penicillin G were found to have the same microbial inhibition due to the negative control. Compared to the IC50 values of Ascorbic acid which was 5 mg/ ml, Curcuma longa methanol extract had a lower IC50 value is 3.2 mg/ml which according to previous literature is a good one given that lower IC50 values indicate great antioxidant activity. It was gathered by the observation that the qualitative extraction of Curcuma longa using methanol shows the presence of varying chemical components such as AR-tumerone, arachinsaeure, alpha-tumerone amongst others. Research data suggest that ar-turmerone has the potential to promote the positive and negative proliferation of tumor cell lines. As a result, Curcuma longa has high potential to be developed into an antibiotic against Methicillin-resistant Staphylococcus aureus and other clinically important bacterial strains in future.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"5 1","pages":"56 - 62"},"PeriodicalIF":1.7,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47758815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Are new genome variants detected in SARS-CoV-2 expected considering population dynamics in viruses? 考虑到病毒的种群动态，是否预计在SARS-CoV-2中检测到新的基因组变异?

IF 1.7

The EuroBiotech Journal Pub Date : 2021-01-01 DOI: 10.2478/ebtj-2021-0001

M. C. Ergoren, P. Tulay, M. Dundar

引用次数: 1

Influence of scarification method on seed germination of the terrestrial orchid Anacamptis laxiflora (Lam.) 刻蚀法对陆生兰种子萌发的影响

IF 1.7

The EuroBiotech Journal Pub Date : 2021-01-01 DOI: 10.2478/ebtj-2021-0004

G. Deconninck, A. Gerakis

{"title":"Influence of scarification method on seed germination of the terrestrial orchid Anacamptis laxiflora (Lam.)","authors":"G. Deconninck, A. Gerakis","doi":"10.2478/ebtj-2021-0004","DOIUrl":"https://doi.org/10.2478/ebtj-2021-0004","url":null,"abstract":"Abstract A critical step during in vitro sexual propagation of terrestrial orchids is the treatment of the microscopic seeds with a disinfecting solution that kills bacteria and fungi attached to the seeds. This treatment is necessary to prevent infection of the culture vessels. At the same time, the treatment serves to scarify the seeds, a process that disrupts seed dormancy and initiates germination. The literature is inconclusive with respect to the proper combination of disinfecting solution strength and treatment duration. Both factors should be adapted to each species to guarantee minimal infection rate without damaging the embryo. This research aims to compare three disinfection/scarification methods for seeds of Anacamptis laxiflora (Lam.): (i) soaking in 0.5% NaClO, (ii) soaking in 0.5% NaClO, then centrifugation, and (iii) presoaking the seeds in sucrose solution, then soaking in 0.5% NaClO. The seeds were soaked in the disinfecting solution for 5 to 85 min. Following scarification, the seeds were sown in modified Malmgren nutrient medium. Infected and germinated vessels were counted at 41 and 189 d after sowing. We found that the longer the chemical treatment, the lower the infection rate, and the higher the germination rate. There was no significant difference in germination rate between the NaClO and the NaClO-plus-centrifugation method; in fact, the slight savings in disinfection time effected by centrifugation were more than offset by the added complexity of the method. Moreover, we found that centrifugation significantly delays germination. The sucrose presoak-plus-NaClO method was superior to plain NaClO, as the sucrose stimulates the germination of microbial spores on the surface of the seeds, making them easier to kill. Perhaps seeds with thicker testa as well as whole immature capsules could benefit even more from the pretreatment in sucrose solution.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"5 1","pages":"15 - 23"},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43464564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Irrational use of antibiotics with representation of antimicrobial resistance patterns in Sudan: a narrative review 非理性使用抗生素与苏丹抗微生物耐药性模式的相关性：叙述性综述

IF 1.7

The EuroBiotech Journal Pub Date : 2021-01-01 DOI: 10.2478/ebtj-2021-0007

N. Abdulgader, Rawan Khidir Abdel Galil, Shahd Yahaya Mohamed Nour Serag Elnour, Tabark Faisal Fadlallah Elhusain, Tagwa Mahmoud Ahmed Osman, M. Elawad, Islam Mogbil Shaikh Idris Mirghani, Ihab B Abdalrahman, S. Elbager

{"title":"Irrational use of antibiotics with representation of antimicrobial resistance patterns in Sudan: a narrative review","authors":"N. Abdulgader, Rawan Khidir Abdel Galil, Shahd Yahaya Mohamed Nour Serag Elnour, Tabark Faisal Fadlallah Elhusain, Tagwa Mahmoud Ahmed Osman, M. Elawad, Islam Mogbil Shaikh Idris Mirghani, Ihab B Abdalrahman, S. Elbager","doi":"10.2478/ebtj-2021-0007","DOIUrl":"https://doi.org/10.2478/ebtj-2021-0007","url":null,"abstract":"Abstract Background Increasing bacterial resistance to antibiotics is a growing menace, mainly caused by the rapid genetic modification of bacterial strains and new alternations in behavior favoring their survival. There is no doubt that the irrational use of antibiotics is one of the factors contributing to the rise of this problem, whether that be in hospitals or at a community level. Although the extent of this influence is yet to be learned, it is definite that this is of great impact on the endemic disease patterns in developing areas specifically and on an expanding global issue generally. Purpose and scope This paper will provide a narrative review of relevant previous publications of antibiotic misuse to portray a clearer picture of its causes and consequences in Sudan. Methodology The PICO method was used by which evidence-based research websites were scanned for key words. Results were assessed for relevance and then critically appraised. All papers included were summarized and presented in a narrative review format. Results From a total of 9 research papers from Pub Med, Scopus, Cochrane and Google Scholar search engines, 7 were selected, presented, and discussed. Conclusion Given the facts of high bacterial resistance that has emerged worldwide catastrophically, the implementation of a meticulous surveillance system designed to restrict the irrational use of antibiotics by the public and health sectors alike with adjunct educational and training programs relevant to the regional epidemiology and economy will massively contribute to a lower resistance rate due to antibiotic misuse.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"5 1","pages":"43 - 47"},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42324743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Vitamin D3 as possible diagnostic marker of Eating Disorders 维生素D3可能是饮食失调的诊断标志

IF 1.7

The EuroBiotech Journal Pub Date : 2021-01-01 DOI: 10.2478/ebtj-2021-0005

V. Bella, Giulia Gizzi, E. Albi, M. Codini, Simonetta Marucci, L. Ragione, T. Beccari, M. R. Ceccarini

{"title":"Vitamin D3 as possible diagnostic marker of Eating Disorders","authors":"V. Bella, Giulia Gizzi, E. Albi, M. Codini, Simonetta Marucci, L. Ragione, T. Beccari, M. R. Ceccarini","doi":"10.2478/ebtj-2021-0005","DOIUrl":"https://doi.org/10.2478/ebtj-2021-0005","url":null,"abstract":"Abstract Purpose Eating Disorders (EDs) refer to a group of psychiatric conditions in which disorderly food intake results in impaired psychological functioning or physical health. Nowadays, these disorders represent an increasing problem in modern society. There are no universally validated clinical parameters to confirm, disprove or simply help to identify EDs except for diagnostic criteria on psychiatric basis. The aim of this study was the assessment of Vitamin D3 level in patients with EDs to understand if it might be a valid clinical biochemistry parameter useful as prognostic marker. Methods The sample consists of 28 female patients, who suffer from EDs. Blood samples were examined in terms of blood count, glucose, cholesterol and Vitamin D3 levels. The other clinical biochemistry parameters were analysed to understand if the Vitamin D3 was the only altered parameter. Results The parameters that appear altered are glycemia, cholesterol and, in particular, Vitamin D3. Significant results were obtained comparing controls with restrictive-type anorexia nervosa (p value= 0,003) and with purging-type anorexia nervosa (p value= 0,007). Conclusion There are currently no universally validated and diagnostic reliable clinical biochemistry parameters for EDs but, in the light of the findings, but our research indicates the potential use of Vitamin D3 as a biomarker for anorexia nervosa. Level of evidence Level III: Evidence obtained from a single-center cohort study.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"5 1","pages":"24 - 33"},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43180793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Recent Advances in 3D Printing of Polyhydroxyalkanoates: A Review 聚羟基烷酸酯3D打印研究进展综述

IF 1.7

The EuroBiotech Journal Pub Date : 2021-01-01 DOI: 10.2478/ebtj-2021-0008

A. Kovalcik

引用次数: 22

Comparison of deep learning and conventional machine learning methods for classification of colon polyp types 深度学习与传统机器学习方法在结肠息肉类型分类中的比较

IF 1.7

The EuroBiotech Journal Pub Date : 2021-01-01 DOI: 10.2478/ebtj-2021-0006

R. S. Doğan, B. Yılmaz

{"title":"Comparison of deep learning and conventional machine learning methods for classification of colon polyp types","authors":"R. S. Doğan, B. Yılmaz","doi":"10.2478/ebtj-2021-0006","DOIUrl":"https://doi.org/10.2478/ebtj-2021-0006","url":null,"abstract":"Abstract Determination of polyp types requires tissue biopsy during colonoscopy and then histopathological examination of the microscopic images which tremendously time-consuming and costly. The first aim of this study was to design a computer-aided diagnosis system to classify polyp types using colonoscopy images (optical biopsy) without the need for tissue biopsy. For this purpose, two different approaches were designed based on conventional machine learning (ML) and deep learning. Firstly, classification was performed using random forest approach by means of the features obtained from the histogram of gradients descriptor. Secondly, simple convolutional neural networks (CNN) based architecture was built to train with the colonoscopy images containing colon polyps. The performances of these approaches on two (adenoma & serrated vs. hyperplastic) or three (adenoma vs. hyperplastic vs. serrated) category classifications were investigated. Furthermore, the effect of imaging modality on the classification was also examined using white-light and narrow band imaging systems. The performance of these approaches was compared with the results obtained by 3 novice and 4 expert doctors. Two-category classification results showed that conventional ML approach achieved significantly better than the simple CNN based approach did in both narrow band and white-light imaging modalities. The accuracy reached almost 95% for white-light imaging. This performance surpassed the correct classification rate of all 7 doctors. Additionally, the second task (three-category) results indicated that the simple CNN architecture outperformed both conventional ML based approaches and the doctors. This study shows the feasibility of using conventional machine learning or deep learning based approaches in automatic classification of colon types on colonoscopy images.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"5 1","pages":"34 - 42"},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44610777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COVID-19 vaccines: Where do we stand? COVID-19疫苗:进展如何?

IF 1.7

The EuroBiotech Journal Pub Date : 2021-01-01 DOI: 10.2478/ebtj-2021-0002

P. Tulay, M. C. Ergoren, M. Dundar

{"title":"COVID-19 vaccines: Where do we stand?","authors":"P. Tulay, M. C. Ergoren, M. Dundar","doi":"10.2478/ebtj-2021-0002","DOIUrl":"https://doi.org/10.2478/ebtj-2021-0002","url":null,"abstract":"Abstract Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) was first reported in the city Wuhan, China in December 2019. The high rates of infection led to quick spread of the virus around the world and on March 11th, 2020, the World Health Organization (WHO) announced the pandemic of the Coronavirus disease 2019 (COVID-19) caused by the SARS-CoV-2. The pharmaceutical companies and institutions have been working towards developing a safe and effective vaccine in order to control the pandemic. The biology of SARS-CoV-2 is briefly discussed describing the transcription of the virus and the receptor recognition. The spike protein of SARS-CoV-2 is important in the attachment of the host cell and RNA-dependent RNA polymerase (RdRp) is involved in the replication of the virus making them good candidates for drug and vaccine targets. To date many different strategies have been employed in the development of vaccines and a number of them are in the phase III of clinical trials with promising results. In this mini-review, we assessed the literature throughly and described the latest developments in SARS-CoV-2 vaccines for humans. The main benefits and drawbacks of each platform is evaluated and the possible changes in the vaccine effectivity due to naturally occuring SARS-CoV-2 mutations have been described.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"5 1","pages":"4 - 7"},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45747711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1