The EuroBiotech Journal最新文献_第10页

Polymerization degree-dependent changes in the effects of in vitro chitosan treatments on photosynthetic pigment, protein, and dry matter contents of Ipomoea purpurea 壳聚糖处理对紫叶光合色素、蛋白质和干物质含量影响的聚合度依赖性变化

IF 1.7

The EuroBiotech Journal Pub Date : 2019-10-01 DOI: 10.2478/ebtj-2019-0024

Ruhiye Kıran Acemi, Arda Acemi

{"title":"Polymerization degree-dependent changes in the effects of in vitro chitosan treatments on photosynthetic pigment, protein, and dry matter contents of Ipomoea purpurea","authors":"Ruhiye Kıran Acemi, Arda Acemi","doi":"10.2478/ebtj-2019-0024","DOIUrl":"https://doi.org/10.2478/ebtj-2019-0024","url":null,"abstract":"Abstract Morning Glory (Ipomoea purpurea (L.) Roth.) is a climbing plant known for its ornamental properties and ease of cultivation in temperate climates. Quality and colour of flowers and leaves, especially in the production of ornamentals, are important parameters both for producers and for customers. This study aimed to investigate the changes in photosynthetic pigment, protein and dry matter content of in vitro-propagated I. purpurea following chitosan treatment with different polymerization degrees (DP) and to determine the indirect effect of this biopolymer on leaves of the plant. Nodal explants of I. purpurea were cultured in medium supplemented with 5, 10 and 20 mg L−1 concentrations of a chitosan oligomers mixture with a variable degree of polymerization (DP) ranging from 2 to 15 or chitosan polymer with DP of 70. It was found that both oligomeric and polymeric chitosan treatments increased chlorophyll-a contents in the leaves when compared to the chitosan-naïve control group. Polymeric chitosan stimulated chlorophyll-b and carotenoid synthesis more effectively than the oligomer mixture. Also, 10 mg L−1 polymeric chitosan better triggered total protein production and plant dry matter content in I. purpurea. The results of this study showed that, due to their stimulatory effects on photosynthetic pigment, protein and plant dry matter production, chitosan oligomers at low concentration and polymers at moderate concentration might be considered as safe and natural biostimulants for ornamental plants which could affect the plant’s attractiveness and commercial success.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"3 1","pages":"197 - 202"},"PeriodicalIF":1.7,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45277883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Photosynthetic light reactions in Oryza sativa L. under Cd stress: Influence of iron, calcium, and zinc supplements 镉胁迫下水稻光合作用的光反应：铁、钙和锌补充剂的影响

IF 1.7

The EuroBiotech Journal Pub Date : 2019-10-01 DOI: 10.2478/ebtj-2019-0021

A. Sebastian, M. Prasad

引用次数: 7

A comparison of machine learning algorithms for the prediction of Hepatitis C NS3 protease cleavage sites 机器学习算法预测丙型肝炎NS3蛋白酶切割位点的比较

IF 1.7

The EuroBiotech Journal Pub Date : 2019-10-01 DOI: 10.2478/ebtj-2019-0020

H. Chown

引用次数: 7

Artificial cell microcapsules containing live bacterial cells and activated charcoal for managing renal failure creatinine: preparation and in-vitro analysis 含有活细菌细胞和活性炭的人造细胞微胶囊用于管理肾功能衰竭肌酐:制备和体外分析

IF 1.7

The EuroBiotech Journal Pub Date : 2019-10-01 DOI: 10.2478/ebtj-2019-0023

T. Lim, W. Ouyang, C. Martoni, Nasri Balit, S. Prakash

引用次数: 0

Proteases with caspase 3-like activity participate in cell death during stress-induced microspore embryogenesis of Brassica napus 具有caspase 3样活性的蛋白酶参与甘蓝型油菜应激诱导小孢子胚胎发生过程中的细胞死亡

IF 1.7

The EuroBiotech Journal Pub Date : 2019-07-01 DOI: 10.2478/ebtj-2019-0018

Eduardo Berenguer, María-Teresa Solís, Y. Pérez-Pérez, P. Testillano

{"title":"Proteases with caspase 3-like activity participate in cell death during stress-induced microspore embryogenesis of Brassica napus","authors":"Eduardo Berenguer, María-Teresa Solís, Y. Pérez-Pérez, P. Testillano","doi":"10.2478/ebtj-2019-0018","DOIUrl":"https://doi.org/10.2478/ebtj-2019-0018","url":null,"abstract":"Abstract Microspore embryogenesis is a model system of plant cell reprogramming, totipotency acquisition, stress response and embryogenesis initiation. This in vitro system constitutes an important biotechnological tool for haploid and doubled-haploid plant production, very useful for crop breeding. In this process, microspores (cells that produce pollen grains in planta) are reprogrammed toward embryogenesis by specific stress treatment, but many microspores die after the stress. The occurrence of cell death is a serious limiting problem that greatly reduces microspore embryogenesis yield. In animals, increasing evidence has revealed caspase proteolytic activities as essential executioners of programmed cell death (PCD) processes, however, less is known in plants. Although plant genomes do not contain caspase homologues, caspase-like proteolytic activities have been detected in many plant PCD processes. In the present study, we have analysed caspase 3-like activity and its involvement in stress-induced cell death during initial stages of microspore embryogenesis of Brassica napus. After stress treatment to induce embryogenesis, isolated microspore cultures showed high levels of cell death and caspase 3-like proteolytic activity was induced. Treatments with specific inhibitor of caspase 3-like activity reduced cell death and increased embryogenesis induction efficiency. Our findings indicate the involvement of proteases with caspase 3-like activity in the initiation and/or execution of cell death at early microspore embryogenesis in B. napus, giving new insights into the pathways of stress-induced cell death in plants and opening a new way to improve in vitro embryogenesis efficiency by using chemical modulators of cell death proteases.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"3 1","pages":"152 - 159"},"PeriodicalIF":1.7,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42541613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Optimization of in vitro asymbiotic seed germination protocol for Serapias vomeracea 山芹体外非共生种子萌发条件的优化

IF 1.7

The EuroBiotech Journal Pub Date : 2019-07-01 DOI: 10.2478/ebtj-2019-0017

Arda Acemi, Fazıl Özen

{"title":"Optimization of in vitro asymbiotic seed germination protocol for Serapias vomeracea","authors":"Arda Acemi, Fazıl Özen","doi":"10.2478/ebtj-2019-0017","DOIUrl":"https://doi.org/10.2478/ebtj-2019-0017","url":null,"abstract":"Abstract Serapias vomeracea is an economically important orchid species which is over-collected from nature, because of its glucomannan-rich tubers. Thus, optimization of in vitro culture methodology in this species is required to meet industrial needs and to secure its populations in nature. This study aimed to optimize the surface sterilization protocol for S. vomeracea seeds and to select the optimal seed germination medium by comparing the commonly used media in in vitro orchid culture. During seed surface sterilization, ethyl alcohol (EtOH) pre-treatment prior to sodium hypochlorite (NaOCl) treatment increased the disinfection success and viable seed yield when examined using the triphenyl tetrazolium chloride (TTC) seed viability test. Also, low-g force centrifugation as an additional step in the surface sterilization method separated the seeds without embryo from the viable seeds and thereby decreased potential counting errors after incubation. Comparison of media showed that solid Knudson C (KN) medium induced the highest number of germinated seeds. However, seed germination success of Lindemann (LN) and Vacin & Went (VW) media was found to be higher when the media was used in liquid form. Half-strength liquid VW was the only medium that induced higher germination success than the other full-strength media. The highest number of ungerminated seeds was found when using KN medium whereas liquid VW medium gave the lowest number. In general, protocorm formation was triggered when the media were used in liquid form. However, rhizoid elongation was suppressed in liquid media. These findings suggest that this optimized seed surface sterilization method offers a simple and effective alternative to classical methods. Additionally, solid KN medium may be considered as a cost-effective and reliable alternative to other commonly-used complex media in S. vomeracea cultures.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"3 1","pages":"143 - 151"},"PeriodicalIF":1.7,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43971921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Biocomposites based on collagen and keratin with properties for agriculture and industrie applications 以胶原蛋白和角蛋白为基础的具有农业和工业用途的生物复合材料

IF 1.7

The EuroBiotech Journal Pub Date : 2019-07-01 DOI: 10.2478/ebtj-2019-0019

M. Niculescu, D. Epure, M. Lasoń-Rydel, C. Gaidău, M. Gidea, C. Enascuta

{"title":"Biocomposites based on collagen and keratin with properties for agriculture and industrie applications","authors":"M. Niculescu, D. Epure, M. Lasoń-Rydel, C. Gaidău, M. Gidea, C. Enascuta","doi":"10.2478/ebtj-2019-0019","DOIUrl":"https://doi.org/10.2478/ebtj-2019-0019","url":null,"abstract":"Abstract In the present research biocomposites based on extracts of collagen and keratin recovered from the leather industry by-products were made and the specific properties for applications in agriculture and industry were studied. To this aim, collagen and keratin have been extracted from bovine leather and sheep wool by-products and have been added and crosslinked with recognized compounds for reduced environmental impact (glycerol, vegetable tanning extract, essential oils with fungicidal properties and insecticides). The biocomposite properties were evaluated on the basis of complex analytical investigations on chemical structure, texture, contact angle, mechanical resistance, water vapor permeability and water absorption, biodegradation, germination and plant biomass growth. The biocomposites have demonstrated pelliculogenic properties and nitrogen controlled release to stimulate germination and nutrition of rape seedlings, which promotes them for agricultural applications, but also other surface properties have been identified, for industrial applications, for example in leather finishing for special destinations. Addition of odorous principles with controlled release recommends this type of biocomposites for environmentally friendly products, maintenance of cleaning, etc.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"3 1","pages":"160 - 166"},"PeriodicalIF":1.7,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44154551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Evolution of phytochemical and antioxidant activity of Tunisian carob (Ceratonia siliqua L.) pods during maturation 突尼斯角豆(Ceratonia siliqua L.)豆荚成熟过程中植物化学和抗氧化活性的演化

IF 1.7

The EuroBiotech Journal Pub Date : 2019-07-01 DOI: 10.2478/ebtj-2019-0016

Khadija Ben Othmen, W. Elfalleh, B. Lachiheb, M. Haddad

{"title":"Evolution of phytochemical and antioxidant activity of Tunisian carob (Ceratonia siliqua L.) pods during maturation","authors":"Khadija Ben Othmen, W. Elfalleh, B. Lachiheb, M. Haddad","doi":"10.2478/ebtj-2019-0016","DOIUrl":"https://doi.org/10.2478/ebtj-2019-0016","url":null,"abstract":"Abstract The Ceratonia siliqua fruits contain several substances known to have high adaptability to environmental conditions. The aim of this paper is to evaluate the changes in physicochemical properties of different Tunisian provenances of carob pulps harvested at three ripening stages. Furthermore, six provenances were investigated during the ripening process in terms of their moisture, ash, minerals, bioactive compounds, antiradical activity and sugar profile. The results demonstrated that all examined parameters are highly influenced by geographic origin. Concerning ripening impact, our data showed that water and ash content significantly decreased during the development of six provenances, as well as the bioactive and mineral contents. Thus, the total polyphenols (TP), total flavonoids (TF) and condensed tannins (CT) contents exhibited the highest levels in the unripe fruits. The antiradical activity trend was positively correlated to the behavior of the bioactive compounds content. Moreover, the sucrose, glucose and fructose were the main sugar qualified and quantified in carob pods at different ripening stages. At the maturity stage, the monosaccharide contents (glucose and fructose) were slightly reduced, while, the sucrose was rapidly accumulated. In conclusion, the ripening process diversely affected the nutritional composition and generally extended the exploitation of carob fruits. The study could provide valuable information about the suitability of carob pods at different maturity stages as potential biomaterials for nutraceutical applications.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"3 1","pages":"135 - 142"},"PeriodicalIF":1.7,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45367986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Corrosion inhibition of iron surfaces with phosphatidic acid 磷脂酸对铁表面的缓蚀作用

IF 1.7

The EuroBiotech Journal Pub Date : 2019-07-01 DOI: 10.2478/ebtj-2019-0015

Ahmed Sameer Al-Badran, A. Mechler

{"title":"Corrosion inhibition of iron surfaces with phosphatidic acid","authors":"Ahmed Sameer Al-Badran, A. Mechler","doi":"10.2478/ebtj-2019-0015","DOIUrl":"https://doi.org/10.2478/ebtj-2019-0015","url":null,"abstract":"Abstract Preventing the corrosion of iron in inaccessible structures requires a coating method that reaches all surface areas and creates a uniform protective layer. An ages old practice to protect iron artefacts is to coat them with animal fat, that is, a mixture of lipids. This “method” is accidentally ingenious: some natural phospholipids found in animal fat have the potential to form a tightly packed self-assembled monolayer on metal oxide surfaces, similar to the surfactant monolayers that have attracted increasing attention lately. Thus, the most primitive corrosion prevention method may point at a way to coat complex iron structures in an industrial environment. Here the ability of phosphatidic acid, a natural lipid, to coat and protect iron surfaces was examined. Iron coated quartz crystal microbalance (QCM) sensors were used for the experiments, to monitor the deposition of the lipid as well as the acidic corrosion (dissolution) of iron in situ, in real time. The sensors were coated by self-assembled monolayers of di-myristoyl phosphatidic acid using the liposome deposition method. In this process, 50-100 nm vesicles formed by the lipid are delivered in an aqueous solution and spontaneously coat the iron surfaces upon contact. QCM and ellipsometry measurements confirmed that continuous bilayer and monolayer surface coatings can be achieved by this method. QCM measurements also confirmed that the layers were corrosion resistant in 0.01M acetic acid solution that would dissolve the thin iron layer in minutes in the absence of the protective coating. XPS results suggested a chemisorption-based mechanism of phosphatidic acid attachment to the iron surface. Hence, liposome deposition of phosphatidic acid offers a suitable solution to coat iron surfaces in inaccessible structures in situ.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"3 1","pages":"128 - 134"},"PeriodicalIF":1.7,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45101684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Defense manifestations of enzymatic and non-enzymatic antioxidants in Ricinus communis L. exposed to lead in hydroponics 水培条件下蓖麻酶和非酶抗氧化剂对铅的防御表现

IF 1.7

The EuroBiotech Journal Pub Date : 2019-07-01 DOI: 10.2478/ebtj-2019-0014

B. Kiran, M. Prasad

{"title":"Defense manifestations of enzymatic and non-enzymatic antioxidants in Ricinus communis L. exposed to lead in hydroponics","authors":"B. Kiran, M. Prasad","doi":"10.2478/ebtj-2019-0014","DOIUrl":"https://doi.org/10.2478/ebtj-2019-0014","url":null,"abstract":"Abstract Lead (Pb) is a major inorganic pollutant with no biological significance and has been a global concern. Phytotoxicity of lead induces toxic effects by generating reactive oxygen species (ROS), which inhibits most of the cellular processes in plants. Hydro-ponic experiments were performed with Ricinus communis to investigate the toxicity and antioxidant responses by exposing to different concentrations of lead (0, 200 and 400 µM) for 10 days. Pb stress caused a significant increase in electrolyte leakage, non-enzymatic antioxidants (phenols and flavonoids) and a decrease in the elemental profile of the plant. Histochemical visualization clearly indicates the significant increase of H2O2 production in dose-dependent manner under Pb stress. Likewise, an increase in catalase, guaiacol peroxidase and superoxide dismutase activity was also evident. Ascorbate peroxidase and MDAR, on the other hand, responded biphasically to Pb treatments showing a decrease in concentration. The decline in redox ratio GSH/GSSG was imposed by the indirect oxidative stress of Pb. Hence these findings showed the ameliorative potential of R. communis to sustain Pb toxicity under oxidative stress.","PeriodicalId":22379,"journal":{"name":"The EuroBiotech Journal","volume":"3 1","pages":"117 - 127"},"PeriodicalIF":1.7,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47304155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9