The Animal Biology最新文献

{"title":"The analysis of the condition of colostral immunity of sows after vaccination against porcine epidemic diarrhea virus","authors":"O. Y. Aуshpur, I. Mushtuk, L. Muzykina, O. Tarasov, O. Yermolenko, V. V. Gumeniuk, M. Derevyanko","doi":"10.15407/ANIMBIOL23.01.003","DOIUrl":"https://doi.org/10.15407/ANIMBIOL23.01.003","url":null,"abstract":"Porcine epidemic diarrhea (PED) is an acute, highly contagious viral disease of pigs of all age groups, but it is manifested by particularly high mortality of newborn piglets and characterized by vomiting, diarrhea, and lack of appetite. Porcine epidemic diarrhea (PED) remains a problem for pig farms in many countries, including Ukraine. Specific prophylaxis PED had proven to be a difficult task and despite many studies, the problem of PED remains unresolved. Since piglets get infected during the first days after birth, their protection can only be provided by colostral antibodies of immuned sows. Therefore, the urgent task of veterinary medicine scientists is to develop effective methods of preventing the disease, which is to use local strains of the pathogen. The article presents the results of studies of colostral immunity of piglets by determining the presence of antibodies against the PED virus in the colostrum of sows who were immunized with a vaccine inactivated against PED from the intestinal virus of artificially infected suckling piglets. Infectious activity of matrix broods of the virus for the production of the preparation was determined by RT-PCR. According to the results of research, material was selected for the manufacture of the vaccine. Subsequently, the drug was monitored according to technological regulations, including the infectious activity of the virus, which ranged from 1.96×10–8 to 3.16×10–9. We conducted a comparative study of the presence of antibodies to the PED virus in the colostrum of sows in the treatment of animals by “back-feeding” and vaccination of sows with inactivated preparation. Samples of colostrum from sows were taken within 1–4 hours after farrowing. The article presents research results that allow us to conclude that both methods of treatment of sows create immunity in newborn piglets. Thus, the presence of antibodies to the PED virus in colostrum in the first hours after birth provide protection against infection and death.","PeriodicalId":22372,"journal":{"name":"The Animal Biology","volume":"51 1","pages":"3-6"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90802716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"L-glutamic acid effect on changes in biochemical parameters of rats intoxicated by carbon tetrachloride","authors":"N. Salyha","doi":"10.15407/ANIMBIOL23.01.018","DOIUrl":"https://doi.org/10.15407/ANIMBIOL23.01.018","url":null,"abstract":"This study aims to explore the ameliorative effects of L-glutamic acid (L-Glu) against carbon tetrachloride (CCl4) toxicity in male rats. Changes in the activities of alanine and aspartate aminotransferases (ALAT, ASAT) were studied in the tissues and blood of rats. Concentrations of creatinine, triacylglycerol and cholesterol were also determined in the blood of experimental animals. Intraperitoneal administration of CCl4 to rats led to changes in the blood of animals of the studied parameters — an increase of the activities of aminotransferases, triacylglycerol and cholesterol levels. At the same time, a decrease of ALAT activity was observed in the tissues of the myocardium and spleen of intoxicated animals, ASAT in their brain, and in the tissues of the lungs there was an increase in the activity of ALAT and the growth of ASAT in the tissues of the spleen In turn, with additional administration to animals intoxicated with tetrachloromethane L-Glu mitigation or no change in most of the studied indicators was observed, namely, in the blood the activity of ASAT fluctuated within the control values, and the concentrations of triacylglycerol and cholesterol did not change compared to the control. Also under the action of the studied aminoacid, no changes were detected in the activities of ALAT in lung tissue and ASAT in spleen tissue. The obtained results indicate a corrective effect of L-Glu on the physiological and biochemical parameters of rats in their intoxication with carbon tetrachloride.","PeriodicalId":22372,"journal":{"name":"The Animal Biology","volume":"33 1","pages":"18-22"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74568681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Streptomyces levoris CNMN-Ac-01 on meat productivity and chemical parameters of rabbit meat","authors":"M. Karaman, Scientific","doi":"10.15407/ANIMBIOL23.01.034","DOIUrl":"https://doi.org/10.15407/ANIMBIOL23.01.034","url":null,"abstract":"The purpose of the work was to evaluate the influence of biomass of Streptomyces levoris CNMN-Ac-01 added to fodder on meat productivity and chemical composition of rabbit meat. The experimental and control groups of animals (each one consisting of 5 rabbits) were formed according to analogous principles of age, sex (only females), body weight, mode of maintenance and feeding. The age of rabbits used for the experiment was 45 days. The control rabbits were fed with granulated fodder produced according to the recipe developed in the laboratory of Nutrition and Forage Technologies. The rabbits in experimental group were fed with the same granulated fodder supplemented with 0.1% biomass of Streptomyces levoris CNMN-Ac-01. The results of the chemical analyzes of the used fodder indicated that the elaborated fodder composition provide rabbits with all necessaries for their vital activity, where as added biomass of Streptomyces levoris CNMN-Ac-01 do not significantly affect its chemical composition. The increase of the protein content by 0.20% and water by 1.15% were observed in meat of experimental rabbits where the content of fat was decreased for 0.71% in comparison to those of the experimental group. The supplementing of streptomycete biomass in granulated fodder had an essential effect on the weight gain of rabbits and improves the bone and meat ratio in the carcass.","PeriodicalId":22372,"journal":{"name":"The Animal Biology","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83435616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The comparative analysis of the methods for keratin extraction from sheep wool and human hair","authors":"V. Havryliak, V. Mykhaliuk","doi":"10.15407/ANIMBIOL22.04.009","DOIUrl":"https://doi.org/10.15407/ANIMBIOL22.04.009","url":null,"abstract":"Nowadays, biopolymers such as keratins are widely used in biomedicine due to their low toxicity, biocompatibility, and biodegradability. At the molecular level, keratins differ from other structural proteins by a high content of disulfide bonds, which provide the formation of a compact three-dimensional structure resistant to biological and chemical degradation. Native keratins are highly ordered, whereas, recovered keratins are characterized by a flexible structure with more accessible functional groups. A characteristic feature of solubilized keratins is their ability to polymerize; therefore, they are widely used to create biomaterials. The extraction of keratins from natural fibers is an important step to the development of functional biomaterials. However, this process is complicated by the presence of a large number of intramolecular and intermolecular disulfide bonds in keratins. That is why keratin extraction by breaking the intermolecular disulfide bonds while preserving the covalent bonds of the polypeptide chain is necessary. The goal of our study was to estimate the different methods of solubilized keratin obtaining. In the experiments, samples of different types of wool and human hair were used. Various methods of keratin extraction were applied. The yield of solubilized keratin (%) was calculated from the ratio of the weight of the lyophilized keratin extract and the initial weight of fibers. The molecular mass of recovered keratins was evaluated by SDS-PAAG electrophoresis in the Laemmli buffer system. An analysis of the efficiency of keratin extraction has shown that solubilized keratin yield ranged from 32% to 51% and depended on the composition of the extraction mixture. Electrophoretic analysis of all keratin extracts obtained by various methods confirmed the presence of two bands, which according to the molecular weight corresponding to I and II types of proteins of intermediate filaments. The presence of these proteins provides self-assembly into complex structures.","PeriodicalId":22372,"journal":{"name":"The Animal Biology","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81140032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary comparative phytochemical screening and antioxidant activity of varieties Vaccinium corymbosum L. (Ericaceae) shoot’ extracts","authors":"N. Yavorska, N. Vorobets, Y. Salyha, O. Vishchur","doi":"10.15407/ANIMBIOL22.04.003","DOIUrl":"https://doi.org/10.15407/ANIMBIOL22.04.003","url":null,"abstract":"Nowadays, the created varieties of Vaccinium corymbosum L. (Ericaceae) are widespread in different regions of all continents with a suitable climate. Until now, mainly the fruits of these plants have been used, and the vegetative aerial part that remains when pruning the bushes has not been employed. Meanwhile, shoots of other species of Ericaceae have long been used as raw materials for the needs of pharmacy, medicine and veterinary medicine. Phytochemical screening methods were used for the detection of various phytochemicals in shoots of three varieties V. corymbosum introduced in Ukraine by qualitative chemical tests to give a general idea regarding the nature of constituents present in plant material, especially with antioxidant activity. Furthermore, the content of extractives was determined in the obtained extracts and their antioxidant activity by determining the total antioxidant capacity using the DPPH radical scavenging method on various stages of plant development. Phytochemical screening on the shoots of three V. corymbosum varieties (Bluejay, Bluecrop, Elliott) showed the presence of carbohydrates, reducing sugars, phenols, flavonoids, tannins, phlobatannins, hydroquinone and arbutin that exhibit antioxidant properties; the extractive value depends on the solvent and stage of plant development. Bluejay in aqueous extracts had the greatest amount of extractives during flowering and at the beginning of the winter period; in Bluecrop it was during fruiting and at the beginning of the winter period; and in Elliott — at the beginning of the winter period. Thus, we can assume the prospects of their study on antimicrobial properties, anti-inflammatory, anti-diabetic, as well as a feed additive for animal feed.","PeriodicalId":22372,"journal":{"name":"The Animal Biology","volume":"112 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72873818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activity of indicators of the humoral immunity unit of calves for the action of pyridoxine hydrochloride","authors":"О. Jaremko, M. Verkholiuk, R. Peleno, V. Semanyuk","doi":"10.15407/ANIMBIOL22.04.018","DOIUrl":"https://doi.org/10.15407/ANIMBIOL22.04.018","url":null,"abstract":"The article presents the data of the effect of different doses of pyridoxine hydrochloride on the activity of humoral immunity in the blood serum of calves of the dairy growing period. The lowest activity of the indicators of humoral immunity was found in the serum of calves for the first day of life in all research groups. Exogenous administration of pyridoxine hydrochloride to colostrum and milk led to changes in the studied parameters. It was found that the bactericidal activity of serum increases on the 21st day of calf life and slightly decreases on the 90th day. The addition of pyridoxine hydrochloride at different doses to the milk leads to a probable increase in bactericidal activity of the serum only at doses of 4.0 mg/kg body weight at 60 and 90 days (P<0.05) and 5.0 mg/kg body weight from 21 90 days (P<0.05). Serum lysozyme activity increases during ontogeny and under the action of exogenous pyridoxine hydrochloride. A significant difference between the lysozyme activity indices of the control and experimental groups was established in calves of II, III, IV and V groups on the 60th and 90th day (P<0.05, P<0.01). Complementary activity of serum increases during ontogenesis, and additional introduction of pyridoxine hydrochloride has led to its decrease. A significant decrease in the complementary activity of serum was detected in calves at doses of 4 mg/kg body weight on the 90th day (P<0.05) and 5 mg/kg body weight from 21st to 90th day (P<0.05, P<0.01).","PeriodicalId":22372,"journal":{"name":"The Animal Biology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84661742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research of acute toxicity and the effect of detergent-disinfectant “Argomol” on the culture of ciliates Tetrahymena pyriformis","authors":"D. Zasiekin, A. G. Pushkova, R. Dymko","doi":"10.15407/ANIMBIOL22.04.022","DOIUrl":"https://doi.org/10.15407/ANIMBIOL22.04.022","url":null,"abstract":"Detergents and disinfectants for sanitation of milking equipment and dairy equipment must have a wide range of antimicrobial activity and provide a proper cleaning effect. At the same time, they must meet high standards of environmental safety and non-toxicity. The article presents data on the parameters of acute toxicity of the new detergent-disinfectant “Argomol”, based on lactic acid, “Katamine AB”, polyhexamethylene guanidine hydrochloride and colloidal silver solution, as well as the toxicity of the tool, which was determined using the express method of Tetrachymena pyriformis. The acute toxicity of the means and its working 0.5% solution in tentative and detailed experiments on white mice was determined and the median lethal dose (DL50) was determined by the method of G. Kerber. It was found that the DL50 of the means in white mice for intragastric administration when calculated by the method of G. Kerber is 4250 mg/kg body weight, and 0.5% of its solution — more than 11000 mg/kg body weight, i. e. according to SOU 85.2-37-736:2011 “Veterinary drugs. Determination of acute toxicity”, this indicates that the means belongs to the IV class of toxicity, which combines low-toxic substances. The results of research on the effect of the new detergent-disinfectant “Argomol” on the culture of ciliates Tetrahymena pyriformis are presented. It was found that the detergent-disinfectant “Argomol” in concentrations of 0.02–0.5% at exposures of 1–10 min did not show toxic effects on the ciliate Tetrahymena pyriformis. Toxicological studies on ciliates suggest that the detergent-disinfectant “Argomol”, when used in the recommended doses and exposures, can be used for sanitation of milking equipment and dairy equipment, as it is environmentally friendly and low-toxic.","PeriodicalId":22372,"journal":{"name":"The Animal Biology","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87371263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"State of enzymatic and non-enzymatic links of antioxidant protection of the liver of tumor-bearing rats under the action of laser irradiation","authors":"O. Ketsa, A. V. Onezhko, M. Marchenko","doi":"10.15407/ANIMBIOL22.04.013","DOIUrl":"https://doi.org/10.15407/ANIMBIOL22.04.013","url":null,"abstract":"The activity of antioxidant enzymes — superoxidedismutase (SOD), catalase and glutathionetransferase, and also the level of low molecular weight antioxidants — vitamin E and ascorbic acid in the liver subcellular fractions of rat with transplanted of Guerin’s carcinoma it was investigated. It is shown that in the liver of tumor-bearing rats in the logarithmic phase of oncogenesis increases the activity of the components of the enzymatic link of the antioxidant system (AOS) and the content of vitamin E with a simultaneous decrease of ascorbic acid. The AOS depletionis expressed by a decrease of antioxidant enzymes activity and a decrease the level of low molecular weight antioxidants in the stationary phase of oncogenesis in the liver cells of tumor-bearing rats. It was found that laser irradiation of rats in the area of tumor growth reduces its effect on liver AOS, which is manifested by increased activity of SOD, catalase and vitamin E content in the stationary phase of Guerin’s carcinoma growth in the body.","PeriodicalId":22372,"journal":{"name":"The Animal Biology","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88145857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laboratory of breeding and animal selection of animals — the important scientific department of the Institute","authors":"E. Fedorovych, V. Fedorovych, N. P. Mazur, M. Kuziv, N. Kuziv, T. Chokan","doi":"10.15407/ANIMBIOL22.03.035","DOIUrl":"https://doi.org/10.15407/ANIMBIOL22.03.035","url":null,"abstract":"The article presents the history of the Laboratory of breeding and selection of animals of the Institute of Animal Biology NAAS, its personnel and scientific achievements. Currently there are seven scientists, including four Doctors of Sciences, two Candidates of Sciences and one junior researcher without a scientific degree work in the laboratory. The team has made radical changes not only in staff capacity but also in research. Scientists have developed and introduced into production methods of optimizing selection and formation of high-value genotypes in dairy cattle, improved methods of targeted breeding of young animals, increasing meat and dairy productivity of animals, assessment of breeding traits of cattle for selection by reproductive and fattening qualities, the efficiency of use in selection process of foreign gene pool. They developed theoretical and practical issues about the genesis of the Ukrainian Black-and-White dairy breed and its western intra-breed type, developed recommendations for the formation of highly productive dairy herds with long economic use, a system for monitoring the genetic variability of dairy breeds cattle, proposed the development of the selection of animals on a set of grounds, discovered the influence of various genetic and paratypic factors on the formation of milk productivity and duration of economic use of cows, etc.","PeriodicalId":22372,"journal":{"name":"The Animal Biology","volume":"171 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79446650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Main directions of research and achievements of the Laboratory of ecological physiology and product quality in 2000–2020","authors":"I. Kovalchuk, R. Fedoruk, M. Tsap","doi":"10.15407/ANIMBIOL22.03.045","DOIUrl":"https://doi.org/10.15407/ANIMBIOL22.03.045","url":null,"abstract":"The article presents the main directions of research and the most significant results of the laboratory of ecological physiology and product quality of the Institute of Animal Biology of NAAS for twenty years of its activity. The results of basic research and applied developments obtained during the implementation of tasks included in the scientific programs of NAAS and the Scientific and Methodological Center “Animal Physiology” are presented. The main characteristics of the physiological impact of new effective feed additives and BAS, tested in production and implemented on different species of animals — cattle, sheep, poultry, rabbits, bees, in pond fish, substantiated methods of their use. The relationship between the degree of man-made pollution and the intensity of physiological and biochemical processes in animals, their adaptive and reproductive capacity. The method of arterio-venous difference in the mammary gland revealed some mechanisms of physiological influence of Cd in the body of cows during lactation under conditions of experimental loading of its salts of different concentrations, the level of biotransformation into milk. The study of the biological action of new organic compounds Se in cows, calves and breeding bulls was started for the first time. The composition of the feed additive based on the drug “Sel-Pleks” was developed and tested, which increases the immunobiological, productive and reproductive ability of the body of cows in the conditions of intensive technogenic load. New scientific data on the biological action of the developed protein-mineral supplements in the body of rabbits with different methods of maintenance and feeding. Physiological and biochemical mechanisms of influence of terms of weaning of young rabbits on immunobiological reactivity of an organism are found out. A recipe for granular feed with high protein content has been developed and its effect on growth intensity and physiological and biochemical processes in rabbits has been studied. Possibilities of application of genetically modified feeds in animal feeding and their influence on physiological status, ecological safety and quality of production are substantiated. The influence of GMO soybeans and products of their processing on the formation of adaptive reactions and functioning of individual organs and systems in animals has been studied. The absence of a pronounced negative effect of transgenic soy on the growth and development of the organism and its reproductive ability in female animals has been proved. The effect of Ge in the form of germanium citrate, obtained by nanotechnological method and chemically synthesized, on the state of the immune, reproductive antioxidant and detoxification systems of the body in laboratory rats of two generations has been studied. The peculiarities of ontogenetic development of male F1 rats under the action of different doses of germanium citrate have been elucidated. The possibility of using small and mediu","PeriodicalId":22372,"journal":{"name":"The Animal Biology","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88216216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}