THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019最新文献

{"title":"Development and experimental evaluation of small concentrated solar oven","authors":"T. A. Ajiwiguna, Narulita Andriyani, Suwandi","doi":"10.1063/1.5138302","DOIUrl":"https://doi.org/10.1063/1.5138302","url":null,"abstract":"In this work, the small scale of solar oven was developed and its performance was evaluated. The dimension of the oven was 13.5 × 13.5 x13.5 cm of length, width, and height respectively. To concentrate the incident radiation, four flat reflectors were installed and the reflected radiation was directed to the oven. The artificial solar radiation which consisted of nine incandescent light bulbs in an array was used as a source of radiation for performance evaluation. 90 grams of water was used as tested material to be heated. The temperature of the water, oven chamber, and ambient were measured by using thermocouples and the radiation intensity was measured by using a solar power meter. Four configurations of the solar oven were compared to investigate the influence of glass cover on the top and insulation layer on the wall of the oven under various intensity of radiation. The experimental results show the highest temperature was achieved when the glass cover and insulation layer was installed at the oven. In this configuration, the increasing water temperature was observed as 7.6 °C and 26.6 °C under radiation intensity of 137 and 880 W/m2 respectively. However, the efficiency of the oven tended to decrease when the radiation intensity was highIn this work, the small scale of solar oven was developed and its performance was evaluated. The dimension of the oven was 13.5 × 13.5 x13.5 cm of length, width, and height respectively. To concentrate the incident radiation, four flat reflectors were installed and the reflected radiation was directed to the oven. The artificial solar radiation which consisted of nine incandescent light bulbs in an array was used as a source of radiation for performance evaluation. 90 grams of water was used as tested material to be heated. The temperature of the water, oven chamber, and ambient were measured by using thermocouples and the radiation intensity was measured by using a solar power meter. Four configurations of the solar oven were compared to investigate the influence of glass cover on the top and insulation layer on the wall of the oven under various intensity of radiation. The experimental results show the highest temperature was achieved when the glass cover and insulation layer was installed at the oven. ...","PeriodicalId":22239,"journal":{"name":"THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86888319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of LC50 value of Nicotiana tabacum L. extract against Gryllus bimaculatus imago and Galleria mellonella larvae","authors":"Hannah Natasha Andjani, Yogi Sentosa, Kori Yati, M. Jufri, A. Fauzantoro, M. Gozan","doi":"10.1063/1.5139361","DOIUrl":"https://doi.org/10.1063/1.5139361","url":null,"abstract":"Replacement of synthetic pesticide with natural pesticide is highly demanding because it is more environmentally friendly. Tobacco is majorly exploited for cigarettes production. Therefore, it needs to be developed for other alternative products, one of which is insecticide due to nicotine content and other toxic compounds. This research aimed to determine the effectiveness of tobacco as an insecticide for Galleria mellonella and Gryllus bimaculatus. The raw material used in this study was the leaf extract of Nicotiana tabacum L., var. Virginia that was obtained by Ethanolic Heat Reflux Extraction (EHRE) technique. After testing on the insects, LC50 values were 36.6 mg/ml for Galleria mellonella and 38.5 mg/ml for Gryllus bimaculatus.Replacement of synthetic pesticide with natural pesticide is highly demanding because it is more environmentally friendly. Tobacco is majorly exploited for cigarettes production. Therefore, it needs to be developed for other alternative products, one of which is insecticide due to nicotine content and other toxic compounds. This research aimed to determine the effectiveness of tobacco as an insecticide for Galleria mellonella and Gryllus bimaculatus. The raw material used in this study was the leaf extract of Nicotiana tabacum L., var. Virginia that was obtained by Ethanolic Heat Reflux Extraction (EHRE) technique. After testing on the insects, LC50 values were 36.6 mg/ml for Galleria mellonella and 38.5 mg/ml for Gryllus bimaculatus.","PeriodicalId":22239,"journal":{"name":"THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87648215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutronic effect of utilizing TRIZO particles on core characteristics of experimental power reactor","authors":"Zuhair, R. A. P. Dwijayanto, H. Adrial, Suwoto, T. Setiadipura","doi":"10.1063/1.5135514","DOIUrl":"https://doi.org/10.1063/1.5135514","url":null,"abstract":"Based on its superior characteristics and to provide core outlet temperature no less than 1000 °C for thermochemical hydrogen production, ZrC becomes a promising candidate to replace SiC coating layer of TRISO coated fuel particles. This paper aimed to study the neutronic effect of utilizing TRIZO coated fuel particles on core characteristics of experimental power reactor. A series of calculations were performed with MCNP6 code and ENDF/B-VII library. The calculation results show that replacement of SiC with ZrC will result in slightly decreased value in effective multiplication reactor (keff), slightly increased value in control rod worth and shutdown margin reactivity, and somewhat more negative value in temperature coefficient of reactivity. The effective delayed neutron fraction (βeff) in ZrC core presents lower values than in SiC core which cause a reduction in the ability to control the reactor. However, the reduced controllability via the lower βeff of ZrC core can be compensated by its favourable temperature coefficient and shutdown margin reactivity. These results conclude that replacing SiC with ZrC particles into experimental power reactor would have considerable impact and benefit from the viewpoint of reactor operational safety.Based on its superior characteristics and to provide core outlet temperature no less than 1000 °C for thermochemical hydrogen production, ZrC becomes a promising candidate to replace SiC coating layer of TRISO coated fuel particles. This paper aimed to study the neutronic effect of utilizing TRIZO coated fuel particles on core characteristics of experimental power reactor. A series of calculations were performed with MCNP6 code and ENDF/B-VII library. The calculation results show that replacement of SiC with ZrC will result in slightly decreased value in effective multiplication reactor (keff), slightly increased value in control rod worth and shutdown margin reactivity, and somewhat more negative value in temperature coefficient of reactivity. The effective delayed neutron fraction (βeff) in ZrC core presents lower values than in SiC core which cause a reduction in the ability to control the reactor. However, the reduced controllability via the lower βeff of ZrC core can be compensated by its favourable ...","PeriodicalId":22239,"journal":{"name":"THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85094056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of fatty pancreas: Acalypha indica Linn. extract as an alternative to simvastatin","authors":"A. Maulidina, Siti Farida","doi":"10.1063/1.5139362","DOIUrl":"https://doi.org/10.1063/1.5139362","url":null,"abstract":"Nowadays, high-fructose/high-cholesterol diet has increased the incidence of dyslipidemia, insulin resistance, and metabolic syndrome, which are the main risk factors of non-alcoholic fatty pancreas disease (NAFPD) and may lead to pancreatic cancer. The pathogenesis and treatment of NAFPD are currently yet to be well-understood. The use of simvastatin in managing the pancreatic disease was shown to reduce cancer risk, but it raised concerns as it was recently found to induce diabetes and worsen hyperglycaemia and A1c levels in pre-existing diabetes. Acalypha indica Linn. (AI) is known to have flavonoids and polyphenols which may decrease LDL and increase HDL level. In contrast to simvastatin, AI may control post-prandial hyperglycemia. A study on rat induced with high-fructose/high-cholesterol diet showed that AI helped reverse fatty formation in the pancreas, which was not significantly different from the rats given simvastatin. This article reviews the potential use of Acalypha indica Linn. extract as an alternative to modify the risk factors underlying NAFPD.Nowadays, high-fructose/high-cholesterol diet has increased the incidence of dyslipidemia, insulin resistance, and metabolic syndrome, which are the main risk factors of non-alcoholic fatty pancreas disease (NAFPD) and may lead to pancreatic cancer. The pathogenesis and treatment of NAFPD are currently yet to be well-understood. The use of simvastatin in managing the pancreatic disease was shown to reduce cancer risk, but it raised concerns as it was recently found to induce diabetes and worsen hyperglycaemia and A1c levels in pre-existing diabetes. Acalypha indica Linn. (AI) is known to have flavonoids and polyphenols which may decrease LDL and increase HDL level. In contrast to simvastatin, AI may control post-prandial hyperglycemia. A study on rat induced with high-fructose/high-cholesterol diet showed that AI helped reverse fatty formation in the pancreas, which was not significantly different from the rats given simvastatin. This article reviews the potential use of Acalypha indica Linn. extract as a...","PeriodicalId":22239,"journal":{"name":"THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84499486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intrahepatic transport of primaquine with OCT1: An in vitro study in HepG2 cells","authors":"M. Louisa, F. Suyatna, S. Wanandi, P. Asih, D. Syafruddin","doi":"10.1063/1.5139373","DOIUrl":"https://doi.org/10.1063/1.5139373","url":null,"abstract":"","PeriodicalId":22239,"journal":{"name":"THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83811208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical and experimental study of translational vibration response’s reduction on aluminum (Al) drilling process using translational mass vibration absorber (TMVA) system","authors":"W. Hendrowati, H. Guntur, A. A. A. Daman, Heny Lestari","doi":"10.1063/1.5138353","DOIUrl":"https://doi.org/10.1063/1.5138353","url":null,"abstract":"The metal working is the main sector of the manufacturing industry. There are many types of machine tools used in the manufacturing industry, one of them are drilling machine. Every working machine will definitely produce the vibration. The vibration that comes from a working drilling machine can reduce the effectiveness of the machine and cause the damage of machine components. And eventually reduce company productivity. The vibration from the drilling machine can be overcome by using Dynamic Vibration Absorber (DVA). The main system of DVA is the mass of absorber and the spring. This study is about an experimental study of Translational Mass Vibration Absorber (TMVA) which is one of Dynamic Vibration Absorber (DVA) types. TMVA is designed to be able to reduce the vertical vibrations caused by the rotational speed of drilling machine. The drilling machine used is Kao Ming Machinery Industrial Co., Ltd type KMR-700DS. TMVA has only vertical direction movement. TMVA is designed in the cylindrical shells made of acrylic. The TMVA consists of the absorber mass and a spring. The main systems of the drilling machine which were being observed in this study were the workpieces and the drill bits. TMVA was only placed on the workpiece during the drilling process. There were 2 variations used, that were the drill size variations and the TMVA mass ratio. The experiment carried out in two different conditions, that were the main system without TMVA and the main system with TMVA. The results are about vibration acceleration response, percentage reduction, surface of the drilling material, and the accuracy level of the drilling diameter for each variation used. TMVA can reduce vibration at the rotational speed of 441 rpm and at the feeding speed of 0.07 mm/rev. Based on the experiment done, it can be concluded that the addition of 1/20 mass of absorber from the workpiece mass represented by 2 coins can effectively reduce the vibration in 12 mm drill size by 31.91%. TMVA also reduce the chatter effect and increase the accuracy level as indicated by the magnitude of the difference of drilling diameter without TMVA and with TMVA optimally at 0.15 mm.","PeriodicalId":22239,"journal":{"name":"THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019","volume":"95 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77703586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards an intelligent reconfigurable BLDC motor controller: Commutation configuration and parameters detection","authors":"A. Mukhlisin, E. Rijanto, I. N. Sutantra, M. N. Yuniarto, A. Pramono","doi":"10.1063/1.5138364","DOIUrl":"https://doi.org/10.1063/1.5138364","url":null,"abstract":"One of the key components in an electric vehicle is brushless direct current (BLDC) motor controller. At present, it is difficult to match a self-made motor controller with several different BLDC motors available in the global market. One of the reasons is that the Hall sensors’ configuration is not well informed. Another reason is the motor parameter’s values used in the controller are not well suited with the real physical conditions of the motor. This study aims to design an intelligent reconfigurable BLDC motor controller. In this paper, a method for automatic commutation configuration and automatic parameter values detection is presented. The Hall sensors and motor phases configurations are identified. The BLDC motor parameter values are detected automatically using mathematical models. Those parameters include internal resistance, inductance, coefficient of back electromotive force (BEMF), torque coefficient, friction coefficient, and moment of inertia. Experimental results of the automatic commutation configuration and parameter values detection are presented. Those results were used to develop a current controller. Further experimental results indicated that the method could be implemented in a BLDC motor current controller.One of the key components in an electric vehicle is brushless direct current (BLDC) motor controller. At present, it is difficult to match a self-made motor controller with several different BLDC motors available in the global market. One of the reasons is that the Hall sensors’ configuration is not well informed. Another reason is the motor parameter’s values used in the controller are not well suited with the real physical conditions of the motor. This study aims to design an intelligent reconfigurable BLDC motor controller. In this paper, a method for automatic commutation configuration and automatic parameter values detection is presented. The Hall sensors and motor phases configurations are identified. The BLDC motor parameter values are detected automatically using mathematical models. Those parameters include internal resistance, inductance, coefficient of back electromotive force (BEMF), torque coefficient, friction coefficient, and moment of inertia. Experimental results of the automatic commutat...","PeriodicalId":22239,"journal":{"name":"THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79462855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural analysis on the hot plenum design of experimental power reactor","authors":"Farisy Yogatama Sulistyo, R. Himawan, A. Nugroho, S. Bakhri, S. Sudadiyo","doi":"10.1063/1.5135542","DOIUrl":"https://doi.org/10.1063/1.5135542","url":null,"abstract":"The bottom reflector plays an important role in the internal core structure of Experimental Power Reactor (RDE). However, it must be able to bear the load either the pebbles that is situated inside the core reactor and from its self-load. The Bottom reflector has column to distribute helium gas from small hole in the cone shaped to through the larger hole in the hot gas chamber. This column, known as hot plenum, is a simple column that consist of circular graphite block. However, this type of structure is vulnerable to collapse. Therefore, this paper will simulate the strength of the bottom reflector based on height of the graphite block that corresponds to the load and analyse Factor of Safety (FOS) to predict the bottom reflector safety. The higher FOS value mean the design is safe. This simulation employs finite elements method under the SolidWork software. The models of hot plenum are named H.250mm, H.200mm, H.150mm, H.100mm and H.50mm, which resulting the minimum FOS of 40.782, 40.891, 41.374, 42.257, 43.394, respectively. Based on calculation among these results, model H.50mm has the maximum value of FOS. This result will be benefit for Experimental Power Reactor design criteria of failure, the design brittle fracture behavior, and the design material selection.The bottom reflector plays an important role in the internal core structure of Experimental Power Reactor (RDE). However, it must be able to bear the load either the pebbles that is situated inside the core reactor and from its self-load. The Bottom reflector has column to distribute helium gas from small hole in the cone shaped to through the larger hole in the hot gas chamber. This column, known as hot plenum, is a simple column that consist of circular graphite block. However, this type of structure is vulnerable to collapse. Therefore, this paper will simulate the strength of the bottom reflector based on height of the graphite block that corresponds to the load and analyse Factor of Safety (FOS) to predict the bottom reflector safety. The higher FOS value mean the design is safe. This simulation employs finite elements method under the SolidWork software. The models of hot plenum are named H.250mm, H.200mm, H.150mm, H.100mm and H.50mm, which resulting the minimum FOS of 40.782, 40.891, 41.374, 42.257...","PeriodicalId":22239,"journal":{"name":"THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79480695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Theoritical approach of pneumatic transportation for fuel pebbles in experimental power reactor development","authors":"K. Widiyati, S. Dibyo","doi":"10.1063/1.5135513","DOIUrl":"https://doi.org/10.1063/1.5135513","url":null,"abstract":"High Temperature Gas Cooled Reactors (HTGR) have many advantages such as inherent safety feature and high efficiency. National Nuclear Agency of Indonesia (BATAN) is developing a small size HTGR which called Experimental Power Reactor (RDE) to generate 10 MW thermal power. On the RDE design development, it is expected that a number of fuel elements are circulated pneumatically in to the reactor core by the fuel handling system every day. The fuel elements are transported vertically in a pneumatic pipe, with length 23 meters. Stability and reliability of the pneumatic transportation play important key factors in the fuel element transportation. In this paper, a theoretical approach to determine the operating pressure and velocity related to the compressed gas carrier in the pneumatic transportation is proposed. The analysis is made by considering several forces acting on the fuel element., such as drag force and force due to fuel mass. Analysis of carrier gas velocity and pressure is made under several stages: during the equilibrium stage and during the lifting stage. This research may provide an important basis in providing the operating parameters in the development of RDE. The principle of drag force and Bernoulli equation were used to determine the operating pressure and velocity to be applied in the development of experimental power reactor. From the calculation, it was obtained that in order to transport a fuel pebbel from the starting pneumatic system, which located in the lower part of pneumatic system, to the outlet pipe of pneumatic system facing the reactor, which located 23 meters above the former location, the velocity required was 1,063.95 m/s. The required pressure to produce such velocity was 39.25 Bar. Since the Fuel Handling System (FHS) equipments were operating at 30 Bar, then the penumatic system would be operating at 9.25 Bar.High Temperature Gas Cooled Reactors (HTGR) have many advantages such as inherent safety feature and high efficiency. National Nuclear Agency of Indonesia (BATAN) is developing a small size HTGR which called Experimental Power Reactor (RDE) to generate 10 MW thermal power. On the RDE design development, it is expected that a number of fuel elements are circulated pneumatically in to the reactor core by the fuel handling system every day. The fuel elements are transported vertically in a pneumatic pipe, with length 23 meters. Stability and reliability of the pneumatic transportation play important key factors in the fuel element transportation. In this paper, a theoretical approach to determine the operating pressure and velocity related to the compressed gas carrier in the pneumatic transportation is proposed. The analysis is made by considering several forces acting on the fuel element., such as drag force and force due to fuel mass. Analysis of carrier gas velocity and pressure is made under several sta...","PeriodicalId":22239,"journal":{"name":"THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019","volume":"113 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75679896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cito-compability analysis of mesenchymal stem cells in platelet rich fibrin matrix (PRFM) for tissue regeneration","authors":"G. Pratama, B. Wiweko, N. Sandora, Evanti Kusumawardhani, D. Rahayu, Kamila Puspita, M. Reksodiputro","doi":"10.1063/1.5139322","DOIUrl":"https://doi.org/10.1063/1.5139322","url":null,"abstract":"Mesenchymal stem cells (MSCs) is progenitor cell that capable to differentiate to various mesenchymal or non-mesenchymal lineages. MSC also play a role towards injury during healing and regeneration processes in most tissue. Platelet rich plasma (PRP) had been reported to induce endometrium regeneration, therefore a compacted form of PRP, known as platelet rich fibrin matrix (PRFM), was studied for its capability to host MSC and the behavior of MSC in the matrix. PRP gelation was generated by adding 25 mM of CaCl2 to form a coin shape with diameter of 5 cm. Each matrix was cut into half, seeded directly with bone marrow (hBM-MSC) and umbilical cord MSC (hUC-MSC) with seeding density of 2,000 cell.cm−2. Initial cellular attachment was analyzed using H&E staining, while the cellular viability was assessed quantitatively using MTT assay and qualitatively using Live/Dead staining. hUC-MSC showed the highest viability compared to hBM-MSC when delivered in PRFM, especially during the first and five days incubation. After 10 dan 15 days incubation, the viability had dropped. The Live/Dead staining indicated too crowded population and most cells were dead. This might be due to asphyxia. hUC-MSC and hBM-MSC were seen to proliferated and infiltrated the PRFM over the time, although the ratio of dead cells was more dominant in the later day of incubation. PRFM can be used to deliver MSC to the site injury. However the direct seeding method was not optimal to hold the cells on place during the first 24 h incubation because they were easily detached.","PeriodicalId":22239,"journal":{"name":"THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78272905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}