Systems Biology in Reproductive Medicine最新文献

{"title":"Permeable cryoprotectants-free vitrification of human TESE, PESA and OAT spermatozoa: clinical outcomes.","authors":"Iryna Kuznyetsova, Sergey I Moskovtsev, Stephanie Ng, Bill Yee, Ayub G M Lulat, Valeriy Kuznyetsov, Clifford L Librach","doi":"10.1080/19396368.2025.2466687","DOIUrl":"10.1080/19396368.2025.2466687","url":null,"abstract":"<p><p>Cryopreservation of testicular and epididymal spermatozoa is more challenging in comparison to ejaculated spermatozoa due to lower sperm concentration and motility, and higher sperm sensitivity to cryoprotectants. Sperm vitrification without the use of potentially toxic permeable cryoprotectants is an attractive freezing alternative for testicular and epididymal spermatozoa, as well as oligoasthenoteratozoospermia (OAT) samples. Our study is a retrospective analysis of outcomes in IVF cycles involving a total of 70 testicular, 77 epididymal and 69 ejaculated OAT samples vitrified in a closed double-straw device using mHTF medium supplemented with protein and sucrose, without any permeable cryoprotectant. In total, 71 frozen samples were used for intracytoplasmic sperm injection (ICSI). Results were compared to fresh samples (26 testicular, 53 epididymal and 63 ejaculated OAT samples) that served as controls. Elective single frozen embryo transfers of euploid or unknown-ploidy blastocysts were performed. While sperm motility is expected to diminish following slow sperm freezing and thawing, our data demonstrated that vitrification of testicular, epididymal and OAT samples had a mean motility rate comparable to fresh samples. No statistically significant differences (<i>p</i> > 0.05) were observed between vitrified versus fresh TESE in fertilization (64.1% vs. 59.5%), blastocyst development (54.9% vs. 56.7%), blastocyst euploidy (36.4% vs. 33.3%), clinical pregnancy (47.8% vs. 36.4%) and live birth rates (43.5% vs. 24.2%). Similarly, vitrified versus fresh PESA showed no statistically significant differences (<i>p</i> > 0.05) in the analyzed results respectively: (69.4% vs.74.9%; 62.6% vs. 59.7%; 40.5% vs. 48.1%; 36.0% vs.37.7%; and 32.0% vs. 27.5%). For vitrified OAT samples, there was a significant difference in blastocyst development and euploidy rates when compared to the control group. Our results demonstrate that human testicular, epididymal spermatozoa and samples with OAT can be successfully vitrified in small volumes in a closed system without using any permeable cryoprotectants, allowing utilization of this technique in clinical settings.</p>","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"71 1","pages":"54-60"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143473055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring ovarian aging: unraveling the link between mitochondria status and oocyte as a determinant of gamete quality.","authors":"Manuel Belli, Marta Gatti, Luigi Sansone, Matteo Antonio Russo, Maria Grazia Palmerini, Stefania Annarita Nottola, Mohammad Ali Khalili, Guido Macchiarelli","doi":"10.1080/19396368.2025.2562633","DOIUrl":"10.1080/19396368.2025.2562633","url":null,"abstract":"<p><p>The variation in reproductive age among individuals is significant, with many cases of infertility involving premature ovarian aging. This issue, combined with the societal trend of delaying childbearing, leads to age-related ovarian dysfunction. Ovarian aging is related to a decline of ovarian reserve, as oocyte quantity, quality, and precocious senescence, and may affect fertility and the overall individual well-being. Mitochondria play a central role in the maintenance of any cell health. Then mitochondrial dysfunctions may be responsible also for a negative impact on the quality, number, and function of oocytes, leading to different age-related reproductive disorders, impaired oogenesis, and embryogenesis. Although a large number of researches have shown clearly that mitochondrial dysfunction and morphology changes affect the maintenance and function of all major organs and tissues, such as the brain, heart, skeletal muscle, liver, and others the mechanisms contributing to early ovarian aging, a decrease of oocyte quality, and infertility remain unclear. In this review, we summarize the role of mitochondrial dysfunction in ovarian aging, presenting recent findings on morpho-functional changes in these organelles, and highlighting how their dysfunction accelerates ovary and cell senescence. We also explore their impact on oocyte functions. The reported data highlight the critical role of mitochondria in maintaining and enhancing oocyte quality, indicating that future studies should further focus on the mechanisms underlying mitochondrial damage and on identifying mitochondrial targets that may offer promising strategies to preserve, recover, and extend fertility in aging women.</p>","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"71 1","pages":"461-484"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel sperm FcR binding assay that assesses sperm fertilizing potential.","authors":"Barb Cohen, John Walsh, Zimu Chen, Sergey Moskovtsev, Erin Schnellinger, Marty Kathrins, H Nadir Ciray, Larry Lipshultz, Paul Turek, Clifford Librach","doi":"10.1080/19396368.2025.2590747","DOIUrl":"https://doi.org/10.1080/19396368.2025.2590747","url":null,"abstract":"<p><p>Freshly ejaculated mammalian sperm have poor fertilizing ability, with fertility only gained after sperm undergo capacitation and the acrosome reaction. To visualize exposed Fc receptors (FcRs), which occur during the acrosome reaction and whose absence has been related to infertility, a novel sperm FcR binding assay (FcR assay) was developed to assess fertilizing potential of sperm in proof-of-concept studies. A competition binding assay between sperm FcR and exogenously added FcR was used to assess whether the FcR was a functioning ligand in bull sperm. Once FcR was confirmed as a functional ligand, time-based expression of FcR was then evaluated in bull and human sperm using the FcR assay. This FcR assay was then used to evaluate fertility outcomes in cattle with cryopreserved intrauterine insemination (IUI) sperm, and to evaluate sperm FcR expression in patients undergoing IUI treatment in a prospective observational study. Time-based analyses of ejaculates from bull and human sperm demonstrated characteristic, reproducible sinusoidal patterns of FcR expression that corresponded to high and low periods of fertility potential in each species. The pregnancy rate in cattle approached statistical significance using the FcR assay results to inform optimum insemination timing windows versus conventional untimed methods (73.0% vs. 68.4%, respectively; <i>p</i> = 0.06; 95% confidence interval [CI]: 0.98, 1.57) with a 4.4% increase in the overall pregnancy rate. In patients undergoing IUI treatment, FcR expression patterns were identified where sperm were at their optimal fertilizing state, with overall pregnancy rates increasing from 21% to 42% (<i>p</i> = 0.01) when inseminations occurred during the windows where the fertilizing potential of the sperm was deemed optimal. These results suggest that sperm fertility potential is quantifiable in semen samples using our novel sperm FcR assay. Importantly, the FcR assay has the ability to identify optimal fertility windows in real-time, and also in the procedure ejaculates.</p>","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"71 1","pages":"630-645"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145725375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viruses and assisted reproductive technologies (ART).","authors":"Marina Leiva, Maria Lopez-Panades, Di Wang, Mar Domingo-Lopez, Judit Castillo, Ignasi Roig, Rafael Oliva, Meritxell Jodar","doi":"10.1080/19396368.2025.2531744","DOIUrl":"https://doi.org/10.1080/19396368.2025.2531744","url":null,"abstract":"<p><p>This comprehensive review reflects our current knowledge of viral infections in the context of assisted reproduction, highlighting the latest research and guidelines. It focuses on the major viral pathogens that have a significant impact on human reproduction. It examines their impact on both male and female reproductive systems, fertility outcomes, and pregnancy, with a particular emphasis on managing the risks of transmission in assisted reproductive technologies (ART). State-of-the-art screening protocols and preventive measures are outlined, highlighting the progress made in minimizing viral transmission and ensuring safe reproductive outcomes. By presenting the current landscape of viral management in reproductive health, this review underscores the need for continued vigilance, research, and innovation to face viral threats.</p>","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"71 1","pages":"246-278"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144761419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early embryo development: the current perspective in molecular evaluation and clinical status.","authors":"Sevastiani Antonouli, Dimitrios Ioannou, Valentina Di Nisio, Christina Messini, Georgia Kokkali, Katerina Chatzimeletiou, Peter Sutovsky, Alexandos Daponte, George Anifandis","doi":"10.1080/19396368.2025.2551009","DOIUrl":"https://doi.org/10.1080/19396368.2025.2551009","url":null,"abstract":"<p><p>Early embryo development and competence mechanisms are paramount to ART's success but are still underexplored in human-relevant animal models. Clinical embryo evaluation remains largely based on subjectively evaluated morphological characteristics. In the current era of biomarkers and low-input high-throughput technologies, it is possible to investigate the multi-complex molecular landscape of the embryo during development from the zygote up to the blastocyst stage. The scope of this article is to review the underlying molecular mechanisms of pre-implantation embryo development. In addition, we briefly explore the latest advances in embryo quality assessment - including molecular and epigenetic evaluation and technical advances such as time-lapse imaging and artificial intelligence - together with clinical challenges for selecting the best embryo for single embryo transfer.</p>","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"71 1","pages":"356-370"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144969880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel homozygous missense variants in <i>SUN5</i> and <i>DNAH10</i> associated with male infertility and oligoasthenoteratozoospermia.","authors":"Qi Fang, Lanxi Ran, Xinying Bi, Song Liu, Jing Wang, Tengyan Li, Jianyong Di, Ye Liu, Fengqin Xu, Binbin Wang","doi":"10.1080/19396368.2025.2500591","DOIUrl":"10.1080/19396368.2025.2500591","url":null,"abstract":"<p><p>Genetic variants are known causes of male infertility and oligoasthenoteratozoospermia (OAT), as shown by knockout mouse models and patients with infertility. However, most OAT cases lack a definitive genetic diagnosis. Peripheral blood and semen samples were collected from a patient with OAT. Semen analysis, Papanicolaou staining, transmission electron microscopy, whole-exome sequencing (WES), Sanger sequencing, and <i>in silico</i> analyses, such as conservative analysis and conformational analyses, were used to investigate the genetic causes of OAT. Semen analysis revealed a notable reduction in sperm count and motility, and defects in sperm morphology. Light and electron microscopy showed numerous defects in the head-to-tail coupling apparatus of the sperm, and a small number of structural defects in the sperm flagella. WES identified two novel homozygous missense variants <i>SUN5</i>: c.G703A/p.A235T and <i>DNAH10</i>: c.A1436G/p.Q479R. The p.A235T and p.Q479R variants were predicted to generate aberrant SUN5 and DNAH10 proteins using <i>in silico</i> analysis. Here, we report the identification of two novel deleterious variants of <i>SUN5</i> and <i>DNAH10</i> associated with OAT, expanding the mutant spectrum of male infertility.</p>","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"71 1","pages":"190-195"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovarian stimulation protocols in fresh cycles and in elective freeze-all cycles.","authors":"Ioannis E Messinis, Christina I Messini, George Anifandis","doi":"10.1080/19396368.2025.2540280","DOIUrl":"https://doi.org/10.1080/19396368.2025.2540280","url":null,"abstract":"<p><p>Ovarian stimulation is applied to women undergoing IVF treatment with the aim of selecting more than one follicle. Until a few years ago, fresh embryo transfer was the norm, but not without the risk of ovarian hyperstimulation syndrome (OHSS). In recent years, vitrification has allowed the successful freezing of all oocytes or embryos, thus favoring multiple embryo transfer in subsequent thawing cycles and eliminating the risk of OHSS. Certainly, excess embryos from fresh embryo transfer cycles can be also frozen and thawed in future transfers. In terms of the number of oocytes needed, research in recent years has shown differences between fresh embryo transfer cycles and elective freezing of all embryos, termed elective freeze-all cycles, with the fresh cycle requiring an optimal number to avoid OHSS, while in elective freeze-all cycles there appear to be no specific upper limit. Consequently, the approach to ovarian stimulation may also differ between the two types of cycles. Although GnRH antagonists are used in both cycles, in elective freezing cycles progestins tend to replace antagonists in preventing the endogenous LH surge. However, it is unclear whether prevention of the LH surge is required in such cycles, since luteinization affecting the endometrium is not clinically relevant, as no embryo transfer occurs. This narrative review describes the current experience in ovarian stimulation for IVF, highlighting the differences between fresh and elective freeze-all cycles and the potentially different approach.</p>","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"71 1","pages":"307-329"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144822599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of testis proteome alterations associated with male infertility in <i>Dcaf17</i>-deficient mice.","authors":"Bhavesh Mistry, Ayodele Alaiya, Raed Abu-Dawud, Nadya Alyacoub, Dilek Colak, Mohamed Rajab, Maha Alanazi, Zakia Shinwari, Hala Ahmed, Thuraya Alharbi, Junaid Kashir, Falah Almohanna, Abdullah Assiri","doi":"10.1080/19396368.2025.2504459","DOIUrl":"https://doi.org/10.1080/19396368.2025.2504459","url":null,"abstract":"<p><p>Disruption of <i>Dcaf17</i> in mice resulted in male infertility with severe spermatogenesis defects. To investigate the molecular basis of infertility phenotype, we examined testicular proteomes of wild-type (WT) and <i>Dcaf17^-/-</i> mice using a mass spectrometry-based approach. We identified 727 and 525 differentially expressed proteins (DEPs) in 3- and 8-week old testes of <i>Dcaf17^-/-</i> mice, respectively, with an adjusted p-value cut-off of ≤ 0.05. Among these, 299 and 298 DEPs had fold change of ≥ 1.5 between WT and <i>Dcaf17^-/-</i> testes at -3- and 8-week old, respectively. In the 3-week old <i>Dcaf17^-/-</i> testes, 59.5% of the DEPs were up-regulated, while 40.5% were down-regulated. Similarly, in the 8-week old <i>Dcaf17^-/-</i> testes, 83.9% and 16.1% DEPs were up-regulated and down-regulated, respectively. Functional annotation and network analyses highlighted that many DEPs were associated with key biological processes, including ubiquitination, RNA processing, translation, protein folding, protein stabilization, metabolic processes, oxidation-reduction processes and sper-matogenesis. Subsequent immunohistochemistry and immunoblotting analyses showed higher ubiquitin levels in <i>Dcaf17^-/-</i> testes compared to WT, suggesting potential impairment in ubiquitin proteasome system (UPS) due to DCAF17 loss of function. Our data provide a basis for further work to elucidate the molecular function(s) of DCAF17 in spermatogenesis and male fertility.</p>","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"71 1","pages":"206-228"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Specific sperm morphological abnormalities and their functional implications in a cohort of Turkish men from a single-center clinical population.","authors":"Duygu Dursunoglu","doi":"10.1080/19396368.2025.2581705","DOIUrl":"https://doi.org/10.1080/19396368.2025.2581705","url":null,"abstract":"<p><p>The role of sperm morphology in male fertilization potential remains unclear. This study aimed to evaluate the incidence of specific sperm morphological abnormalities in a cohort of Turkish men attending a single-center infertility clinic and to assess their associations with semen quality and sperm functionality. A total of 2,923 men aged 17-57 years were included in this cross-sectional study. Semen parameters and specific morphological abnormalities in the head, neck-midpiece, tail, and cytoplasmic residue were analyzed according to WHO criteria. Participants were categorized into normal and low semen parameter groups based on sperm count, motility, and normal morphology. Associations between semen parameters and morphological abnormalities were assessed using Spearman correlation and binary logistic regression analyses. Head defects were the most prevalent abnormalities, followed by tail, neck-midpiece, and cytoplasmic residue. Normal sperm morphology and specific abnormalities were significantly associated with semen quality, with normal forms demonstrating the strongest predictive potential. Head defects were primarily associated with teratozoospermia, whereas neck-midpiece and tail defects were linked to motility impairments. Notably, round and tapered heads, bent necks, and coiled or short tails showed the strongest associations with progressive and rapid progressive motility, which are critical determinants of fertilization capacity. Our findings indicate that different categories of sperm abnormalities have distinct predictive potentials for semen quality disorders and suggest that specific defects may arise from disruptions in different stages of sperm morphogenesis.</p>","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"71 1","pages":"574-588"},"PeriodicalIF":2.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145471993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SBiRM: future innovation and practice in Personalized and Precision Reproductive Medicine.","authors":"Stephen A Krawetz, B Charlotte","doi":"10.1080/19396368.2024.2447691","DOIUrl":"https://doi.org/10.1080/19396368.2024.2447691","url":null,"abstract":"","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"71 1","pages":"1"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143034211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}