Systems Biology in Reproductive Medicine最新文献_第2页

Busulfan affects the transcript levels of mitochondrial dynamics regulators without cytotoxicity in rat Sertoli cells. Busulfan影响大鼠Sertoli细胞中线粒体动力学调节因子的转录水平而不产生细胞毒性。

IF 2 4区医学

Systems Biology in Reproductive Medicine Pub Date : 2026-12-01 Epub Date: 2026-10-13 DOI: 10.1080/19396368.2026.2741742

Nazila Farhangzad, Oya Sena Aydos, Tulin Ozkan, Meral Tiryaki, Asuman Sunguroğlu, Kaan Aydos

{"title":"Busulfan affects the transcript levels of mitochondrial dynamics regulators without cytotoxicity in rat Sertoli cells.","authors":"Nazila Farhangzad, Oya Sena Aydos, Tulin Ozkan, Meral Tiryaki, Asuman Sunguroğlu, Kaan Aydos","doi":"10.1080/19396368.2026.2741742","DOIUrl":"https://doi.org/10.1080/19396368.2026.2741742","url":null,"abstract":"Busulfan is a widely used alkylating chemotherapeutic agent known for its pronounced gonadotoxic effects, primarily attributed to the depletion of spermatogenic cells. However, the direct cellular responses of testicular somatic cells, particularly Sertoli cells (SCs), remain poorly understood. While busulfan has been reported to induce apoptosis in various proliferative cell types, accumulating in vivo evidence suggests that SCs exhibit resistance to chemotoxic injury. In this study, we investigated the direct effects of busulfan in vitro on freshly isolated primary SCs, with a particular focus on cell survival and mitochondrial dynamics, independent of germ-cell interactions. Our results demonstrate that busulfan does not induce apoptotic cell death in primary SCs, as evidenced by unchanged caspase activity and Annexin V staining following busulfan treatment. Instead, busulfan exposure leads to a distinct remodeling of mitochondrial dynamics. Key mitochondrial fission regulators, including Fis1 and Drp1, were significantly downregulated, indicating suppression of mitochondrial fragmentation. Concurrently, increased expression of the Mfn1 and Opa1 as key mediator of mitochondrial fusion was associated with reduced MTT assay readouts, reflecting a metabolically restrained state rather than cytotoxicity. This interpretation was further supported by decreased expression of P53 and other senescence-associated markers, suggesting attenuation of stress and DNA damage responses. Collectively, these findings indicate that SCs respond to busulfan exposure by adopting fusion-dominant, survival-oriented metabolic program that preserves mitochondrial integrity and cellular viability. This study provides direct evidence for the intrinsic resilience of SCs and highlights mitochondrial dynamics as a key adaptive mechanism underlying their resistance to busulfan-induced toxicity.","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"72 1","pages":"328-344"},"PeriodicalIF":2.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Defining optimal frame rate for kinematic semen analysis of different dog breeds assessed by computer-assisted sperm analysis. 用计算机辅助精子分析确定不同犬种精液运动分析的最佳帧率。

IF 2 4区医学

Systems Biology in Reproductive Medicine Pub Date : 2026-12-01 Epub Date: 2026-05-14 DOI: 10.1080/19396368.2026.2662250

Francisco Sevilla, Marcos Campos, Ignacio Araya-Zúñiga, Almudena García-Molina, Carine Dahl Corcini, Alejandro Saborío-Montero, Carles Soler, Arcesio Salamanca-Carreño, Antonio Sergio Varela Junior, Anthony Valverde

{"title":"Defining optimal frame rate for kinematic semen analysis of different dog breeds assessed by computer-assisted sperm analysis.","authors":"Francisco Sevilla, Marcos Campos, Ignacio Araya-Zúñiga, Almudena García-Molina, Carine Dahl Corcini, Alejandro Saborío-Montero, Carles Soler, Arcesio Salamanca-Carreño, Antonio Sergio Varela Junior, Anthony Valverde","doi":"10.1080/19396368.2026.2662250","DOIUrl":"10.1080/19396368.2026.2662250","url":null,"abstract":"The development of computer-assisted sperm analysis (CASA) systems has expanded the number of analyzable seminal parameters. The aim of this study was to define the optimal frame rate analysis using CASA-Mot system, and to evaluate the effect of different dog breeds on the sperm kinematic variables measured. Ninety-seven ejaculates from 49 adult males of 11 dog breeds enrolled in an artificial insemination program were collected, extended and analyzed at 37 °C. Samples were obtained via manual stimulation at the Sangüeso Veterinary Clinic in Valencia, Spain, and were immediately analyzed using the ISAS®v1 system. Videos were acquired at 500 frames per second (fps) and computationally down sampled to 25-400 fps. Sperm kinematic variables were modeled using an exponential regression to estimate the frame rate required to reach 95% of the asymptotic value. Curvilinear velocity (VCL) was the most frame-rate sensitive parameter. The optimal frame rate calculated from the kinematic variable VCL varied among breeds, ranging from 433.72 fps for the Miniature Schnauzer to 939.67 fps for the Golden Retriever. Furthermore, significant differences were found in all analyzed sperm kinematic parameters between breeds. Marked breed effects were detected in motility, concentration and kinematic patterns at 500 fps, with Jack Russell, French Bulldog, Giant Schnauzer and Golden Retriever showing the highest total and fast motility, and Dachshund and Miniature Schnauzer the lowest. Overall, dogs require higher frame rates than most domestic species for reliable CASA-based kinematic assessment, and breed effects should be considered in future research when interpreting canine semen data.","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"72 1","pages":"268-281"},"PeriodicalIF":2.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147943012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms of folliculogenesis and oogenesis. 卵泡发生和卵子发生的分子机制。

IF 2 4区医学

Systems Biology in Reproductive Medicine Pub Date : 2026-12-01 Epub Date: 2026-04-02 DOI: 10.1080/19396368.2026.2631558

Mohamed Aboul Ezz, Ahmed Zaky Balboula

{"title":"Molecular mechanisms of folliculogenesis and oogenesis.","authors":"Mohamed Aboul Ezz, Ahmed Zaky Balboula","doi":"10.1080/19396368.2026.2631558","DOIUrl":"10.1080/19396368.2026.2631558","url":null,"abstract":"Folliculogenesis is a complex, multi-stage process crucial for the establishment and maintenance of female fertility through the production of a developmentally competent oocyte. Folliculogenesis, including follicular formation, activation, growth and maturation, relies on a finely tuned spatiotemporal crosstalk between germ cells, somatic cells, and the hypothalamic-pituitary-ovarian axis. This work provides a comprehensive overview of the cellular dynamics and molecular mechanisms underlying each stage of follicular development. A particular emphasis is placed on the interaction of growth factors, transcriptional networks, signaling pathways and endocrine cues that collectively govern follicular fate and oocyte quality. Disruptions in these interactions lead to emergence of pathological conditions such as premature ovarian insufficiency and age-related infertility. We further highlight the dual aspects of oocyte maturation, nuclear and cytoplasmic, as major determinants of developmental competence, and explore the role of spindle dynamics, organelle redistribution and epigenetic reprograming in this process. The bidirectional communication between oocytes and cumulus cells, mediated by paracrine signaling and jap junctions, is underscored as a pivotal regulator of oocyte metabolic activity, redox homeostasis, and meiotic competence. A better understanding of the oocyte-cumulus cell interaction offers new approaches for refining the in vitro maturation systems and improving assisted reproductive technologies. A special attention is given to the emerging use of cumulus cell-derived biomarkers for noninvasive assessment of oocyte quality and prediction of preimplantation embryo development. Taken together, this article presents an integrated framework to guide future research in reproductive biology, regenerative medicine, and fertility preservation.","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"72 1","pages":"211-240"},"PeriodicalIF":2.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147609207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel DNAH1 variants in Chinese males with MMAF-associated asthenoteratozoospermia. 中国男性maff相关弱异卵精子症患者的新型DNAH1变异

IF 2 4区医学

Systems Biology in Reproductive Medicine Pub Date : 2026-12-01 Epub Date: 2026-01-30 DOI: 10.1080/19396368.2026.2619009

Kun Ye, LeiYu Deng, Fei Huang, Feiyan Mao, Jinhao Liu, Erkai Liu, Hongbo Zhang, Jianlin Chen, Jia Deng, Aimin Deng, Zenghui Mao, Hualin Huang

{"title":"Novel DNAH1 variants in Chinese males with MMAF-associated asthenoteratozoospermia.","authors":"Kun Ye, LeiYu Deng, Fei Huang, Feiyan Mao, Jinhao Liu, Erkai Liu, Hongbo Zhang, Jianlin Chen, Jia Deng, Aimin Deng, Zenghui Mao, Hualin Huang","doi":"10.1080/19396368.2026.2619009","DOIUrl":"10.1080/19396368.2026.2619009","url":null,"abstract":"Asthenoteratozoospermia is a major contributor to male infertility, with multiple morphological abnormalities of the flagellum (MMAF) representing a genetically heterogeneous disorder characterized by structural defects in sperm flagella. To identify the genetic determinants underlying MMAF-associated infertility, we conducted a comprehensive and systematic investigation involving Chinese infertile couples exhibiting the MMAF phenotype and undergoing assisted reproductive technology (ART). Our integrated approach combined whole-exome sequencing (WES) with Sanger sequencing for variant validation, complemented by scanning and transmission electron microscopy (SEM/TEM) to elucidate ultrastructural features. Molecular analyses included quantitative real-time PCR (qRT-PCR) and immunofluorescence (IF) to evaluate both transcriptional and translational alterations. We identified novel variants in six loci of dynein axonemal heavy chain 1 (DNAH1), including both missense and frameshift variants, across three unrelated families. Affected spermatozoa demonstrated characteristic morphological and ultrastructural abnormalities, while qRT-PCR and IF analyses revealed altered expression patterns of DNAH1. Personalized ART strategies enabled successful pregnancies in individuals harboring DNAH1 variants. While the limited sample size reflects the rarity of this genetic disorder, functional validation beyond expression analysis and structural prediction remains limited. Larger cohorts and in-depth biochemical assays will be required to generalize the findings. Nonetheless, our findings provide important insights into the genetic mechanisms of MMAF and its clinical management.","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"72 1","pages":"116-130"},"PeriodicalIF":2.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146094171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracing refractile body biogenesis in human oocytes: the role of lysosomes and mitochondria. 追踪人卵母细胞的折射体生物发生：溶酶体和线粒体的作用。

IF 2.2 4区医学

Systems Biology in Reproductive Medicine Pub Date : 2026-12-01 Epub Date: 2026-01-16 DOI: 10.1080/19396368.2025.2610348

Yuto Aoki, Xingqiang Wei, Mikiya Nakatsuka, Junko Otsuki

{"title":"Tracing refractile body biogenesis in human oocytes: the role of lysosomes and mitochondria.","authors":"Yuto Aoki, Xingqiang Wei, Mikiya Nakatsuka, Junko Otsuki","doi":"10.1080/19396368.2025.2610348","DOIUrl":"10.1080/19396368.2025.2610348","url":null,"abstract":"Refractile bodies (RBs) in human primordial oocytes may represent a lysosomal mechanism of cellular waste management independent of hormonal stimulation or age. This observational study investigated the ultrastructural features and molecular characteristics of refractile bodies (RBs) in human primordial oocytes, focusing on the involvement of lysosomes, autophagy, and mitochondria. Ovarian tissues were obtained from 34 individuals undergoing oophorectomy as part of female-to-male gender-affirming surgery, with no clinical interventions applied. Using fluorescence microscopy, immunocytochemistry, and transmission electron microscopy (TEM), we found large RBs (>5 μm) in all individuals, with no correlation to age. RBs exhibited strong LysoTracker fluorescence, indicating acidic content. LC3, but not RAB7, colocalized with RBs, suggesting incomplete autophagic processing. TEM revealed lysosomal vesicles, mitochondrial remnants, and lipid-rich structures within RBs, some partially enclosed by isolation membranes. These features support a model in which RBs transition from passive lipid accumulation to autophagy-driven remodeling in a size-dependent manner. RBs displayed lipofuscin-like characteristics and are likely formed through lysosomal and mitophagic pathways. Their formation appears to involve both canonical and non-canonical autophagic mechanisms, independent of age or hormonal stimulation. A limitation of this study is its observational nature without functional validation.","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"72 1","pages":"73-86"},"PeriodicalIF":2.2,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145990504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic and surveillance strategies for Marburg virus persistence in the male reproductive tract: insights from Ebola and Zika viruses. 马尔堡病毒在男性生殖道持续存在的诊断和监测战略：来自埃博拉和寨卡病毒的见解

IF 2 4区医学

Systems Biology in Reproductive Medicine Pub Date : 2026-12-01 Epub Date: 2026-04-16 DOI: 10.1080/19396368.2026.2652242

Martin Ndayambaje, Karim Zaher, Hicham Wahnou, Patrick Gad Iradukunda, Thierry Habyarimana, Pierre Gashema, Olivier Uwishema, Enos Moyo, Mohd Imran, Tafadzwa Dzinamarira, Emmanuel Edwar Siddig, Ayman Ahmed, Abdellah Naya, Mounia Oudghiri, Pacifique Ndishimye, Claude Mambo Muvunyi

{"title":"Diagnostic and surveillance strategies for Marburg virus persistence in the male reproductive tract: insights from Ebola and Zika viruses.","authors":"Martin Ndayambaje, Karim Zaher, Hicham Wahnou, Patrick Gad Iradukunda, Thierry Habyarimana, Pierre Gashema, Olivier Uwishema, Enos Moyo, Mohd Imran, Tafadzwa Dzinamarira, Emmanuel Edwar Siddig, Ayman Ahmed, Abdellah Naya, Mounia Oudghiri, Pacifique Ndishimye, Claude Mambo Muvunyi","doi":"10.1080/19396368.2026.2652242","DOIUrl":"10.1080/19396368.2026.2652242","url":null,"abstract":"The recent outbreak of the Marburg virus (MARV) in Rwanda was effectively controlled, but concerns remain regarding the virus's persistence and its long-term effects. MARV can remain in the testes of nonhuman primates, where it harms Sertoli cells and compromises the blood-testis barrier, which may have implications for male infertility. This persistence is aided by immunosuppressive regulatory T cells (Tregs), which allow the virus to evade the immune system. Drawing comparisons with Ebola and Zika viruses, we propose that MARV may contribute to male infertility and recommend further studies on human and nonhuman primate survivors. Quick and precise diagnosis is vital for controlling outbreaks. Current diagnostic methods include RT-PCR, ELISA (for antibody and antigen detection), virus isolation, and electron microscopy. Newer technologies like multiplex real-time PCR and CRISPR-based biosensors (e.g., SHERLOCK) provide faster, more sensitive, and on-site detection, which is especially useful in resource-limited areas. These advancements improve MARV monitoring; however, strict biosafety measures are still necessary due to its classification as a Risk Group 4 pathogen.","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"72 1","pages":"241-251"},"PeriodicalIF":2.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147692256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fertilization and oocyte activation: overview, updates, and novel hypotheses from a molecular perspective. 受精和卵母细胞活化：综述、最新进展和分子视角下的新假设。

IF 2 4区医学

Systems Biology in Reproductive Medicine Pub Date : 2026-12-01 Epub Date: 2026-01-25 DOI: 10.1080/19396368.2026.2618253

Valentina Di Nisio, Peter Sutovsky, Sevastiani Antonouli, Christina Messini, Katerina Chatzimeletiou, Georgia Kokkali, George Anifandis

{"title":"Fertilization and oocyte activation: overview, updates, and novel hypotheses from a molecular perspective.","authors":"Valentina Di Nisio, Peter Sutovsky, Sevastiani Antonouli, Christina Messini, Katerina Chatzimeletiou, Georgia Kokkali, George Anifandis","doi":"10.1080/19396368.2026.2618253","DOIUrl":"10.1080/19396368.2026.2618253","url":null,"abstract":"The multifaceted process of fertilization encompasses a precisely orchestrated cascade of molecular and morphological modifications of the participating male and female gametes. Fertilization culminates in the union of the maternal and paternal genomes and ultimately spark the development of a new individual. During the last decade, extensive new knowledge has been gained concerning the candidate cellular and molecular mechanisms that guide each step of the process from the sperm journey through the female reproductive tract to the gamete fusion and oocyte activation. In this review, we aim at summarizing the main molecular mechanisms of sperm and oocyte activation and fertilization from the moment sperm enters the female reproductive system up to zygote formation. Focusing on molecular determinants including but not limited to ligands, receptors, and signal transducers, we describe well established and novel molecular candidates that pave the way throughout this complex process and highlight the need for further investigation toward clinical application in assisted reproductive therapy.","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"72 1","pages":"101-115"},"PeriodicalIF":2.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146047035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible mechanisms and clinical innovations of sequential embryo transfer in assisted reproduction. 序贯胚胎移植辅助生殖的可能机制及临床创新。

IF 2 4区医学

Systems Biology in Reproductive Medicine Pub Date : 2026-12-01 Epub Date: 2026-01-11 DOI: 10.1080/19396368.2025.2599792

Lina Ma, He Zhu, Pengyun Zhu, Yihang Wu, Kangzi Yi, Liang Su

{"title":"Possible mechanisms and clinical innovations of sequential embryo transfer in assisted reproduction.","authors":"Lina Ma, He Zhu, Pengyun Zhu, Yihang Wu, Kangzi Yi, Liang Su","doi":"10.1080/19396368.2025.2599792","DOIUrl":"10.1080/19396368.2025.2599792","url":null,"abstract":"This review comprehensively discusses the innovative value and scientific mechanism of sequential embryo transfer (SET) technology in assisted reproduction. SET, by transferring embryos in stages, offers a novel approach to address clinical challenges in patients with recurrent implantation failure (RIF) and poor ovarian response (POR). The core mechanism involves bidirectional signal regulation between the embryo and endometrium. Extracellular vesicles (EVs) released by embryos during the initial transfer contain bioactive molecules like proteins, microRNA (miRNA), and mRNA. Through bidirectional molecular communication, these EVs interact with the maternal-fetal interface, promoting endometrial decidualization and establishing a molecular memory for 'embryo pre-adaptation,' thereby enhancing endometrial receptivity. Subsequent embryo transfers further optimize the pregnancy microenvironment through cumulative signaling effects, boosting embryo development and implantation success rates. Additionally, the synergistic impact of mechanical injury plays a role. Local micro-injuries induced by the transfer catheter operation trigger an inflammatory response, recruit immune cells, activate tissue repair pathways, stimulate regenerative cell and signaling molecule secretion, and enhance angiogenesis and endometrial remodeling. This 'microtrauma pre-activation' fosters favorable conditions for subsequent embryo implantation. Clinical evidence demonstrates that SET significantly elevates the clinical pregnancy and live birth rates in RIF patients without heightening the risk of multiple pregnancies. In POR patients, SET can reduce cycle cancelation rates. This review not only supports the clinical application of SET but also advances assisted reproduction from empirical to precision medicine guided by molecular mechanisms.","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"72 1","pages":"55-66"},"PeriodicalIF":2.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145953047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A concise overview of mammalian spermatogenesis. 哺乳动物精子发生的简明概述。

IF 2 4区医学

Systems Biology in Reproductive Medicine Pub Date : 2026-12-01 Epub Date: 2026-01-09 DOI: 10.1080/19396368.2025.2593339

Tasrin Sultana, Peter Sutovsky

{"title":"A concise overview of mammalian spermatogenesis.","authors":"Tasrin Sultana, Peter Sutovsky","doi":"10.1080/19396368.2025.2593339","DOIUrl":"10.1080/19396368.2025.2593339","url":null,"abstract":"The male gamete, the spermatozoon, is the carrier of paternal inheritance. Besides paternal genome, the spermatozoon contributes essential factors for successful fertilization, embryonic development, and continuation of species. A dynamic and extraordinarily complex process called spermatogenesis produces spermatozoa capable of maturing and acquiring full fertilizing capacity. Spermatogenesis proceeds through a series of events that involve cell division (proliferative phase assuring continuous restocking of spermatogenic stem cells), meiosis (recombination and haploidization of paternal genome), post-meiotic differentiation (spermiogenesis; hypercondensation of sperm DNA and generation of accessory sperm structures), and post-testicular maturation (including epididymal maturation, acquisition of seminal plasma components at ejaculation and spermatozoa capacitation within the female oviduct). In this chapter, we provide a concise overview of testis organization and spermatogenesis in different mammalian species and the details of the intricate structure of human/mammalian spermatozoa. Additionally, we explore the fascinating process of spermatogenesis and its regulation. We thus aim to offer insights into the fundamental process that drives male fertility and provides the foundation of reproductive success.","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"72 1","pages":"3-22"},"PeriodicalIF":2.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145946480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune cell enrichment characteristics and related key genes in intrauterine adhesion: insights from RNA-sequencing analysis. 免疫细胞富集特征及宫内粘连相关关键基因：来自rna测序分析的见解。

IF 2 4区医学

Systems Biology in Reproductive Medicine Pub Date : 2026-12-01 Epub Date: 2026-09-20 DOI: 10.1080/19396368.2026.2731896

Xue Feng, Lili Yu

{"title":"Immune cell enrichment characteristics and related key genes in intrauterine adhesion: insights from RNA-sequencing analysis.","authors":"Xue Feng, Lili Yu","doi":"10.1080/19396368.2026.2731896","DOIUrl":"https://doi.org/10.1080/19396368.2026.2731896","url":null,"abstract":"Intrauterine adhesion (IUA) represents a prevalent cause of persistent infertility and continues to be a significant unresolved challenge in reproductive medicine. However, its full pathogenic mechanisms remain incompletely elucidated. We retrieved endometrial RNA-sequencing data from IUA patients and normal controls in the Gene Expression Omnibus (GEO) database, and screened 586 differentially expressed genes (DEGs) in the endometrium of IUA patients using the R package limma. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and gene set enrichment analysis (GSEA) revealed activation of immune-related pathways, including chronic inflammatory response, complement activation, and complement and coagulation cascades in IUA. Further single-sample gene set enrichment analysis (ssGSEA)-based relative immune cell enrichment analysis revealed decreased enrichment scores of activated CD4 T cells and type 2 T helper cells, alongside increased enrichment scores of effector memory CD8 T cells, macrophages, mast cells, natural killer cells, and T follicular helper cells in IUA patients, indicating their potential involvement in IUA pathogenesis. A protein-protein interaction (PPI) network was constructed, identifying 10 hub genes associated with IUA: CDK1, CCNB1, CCNA2, CENPA, BUB1, AURKB, KIF11, BUB1B, CDC20, and TOP2A. The expression patterns of these genes were further validated in two independent external IUA cohorts, supporting a potential correlative link between these genes and IUA pathogenesis. Our findings provide exploratory insights and potential candidate molecules for subsequent mechanistic studies of IUA onset and progression.","PeriodicalId":22184,"journal":{"name":"Systems Biology in Reproductive Medicine","volume":"72 1","pages":"315-327"},"PeriodicalIF":2.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0