Systematic Entomology最新文献

{"title":"Phylogeny of Membracoidea (Hemiptera: Auchenorrhyncha) based on transcriptome data","authors":"Yanhua Hu,&nbsp;Christopher H. Dietrich,&nbsp;Rachel K. Skinner,&nbsp;Yalin Zhang","doi":"10.1111/syen.12563","DOIUrl":"10.1111/syen.12563","url":null,"abstract":"<p>The suborder Auchenorrhyncha (“true hoppers”) comprises nearly half of known Hemiptera, with &gt;43,000 known species of sap-sucking herbivores distributed worldwide, including many important agricultural pests and vectors of plant disease. More than half of the known Auchenorrhyncha belong to superfamily Membracoidea (leaf- and treehoppers), which has been a source of phylogenetic contention for many years. To construct an improved backbone phylogeny of this superfamily, we obtained transcriptome data for multiple representatives of all 5 previously established extant families and nearly all subfamilies to test their monophyly and relationships. 138 taxa (132 Membracoidea and 6 outgroups) were sampled with an emphasis on families Cicadellidae and Membracidae, which were paraphyletic as previously defined by most authors, several problematic subfamilies (Aphrodinae, Eurymelinae, Ledrinae, Nicomiinae, Stegaspidinae and Tartessinae). We analysed different combinations of data sets (amino acid, complete nucleotide and degeneracy-coded nucleotide) using different modelling schemes. The resultant trees based on different analyses are congruent in most nodes. Discordant nodes mainly pertain to relationships among cicadellid subfamilies and tribal relationships within Aphrodinae and Eurymelinae. Analyses of gene- and site concordance factors and quartet scores indicate that this instability is largely attributable to an overall lack of informative characters across genes and sites rather than strongly supported conflict among genes. According to the congruent nodes, we make the following revisions: combine Stegaspidinae and Centrotinae into a single subfamily, Centrotinae sensu lato; restore Stenocotini from Tartessinae to its original position in the Ledrinae; and transform <i>Holdgatiella</i> Evans from Nicomiinae to Melizoderinae. In addition, to solve the paraphyly of both Cicadellidae and Membracidae, a preferred option would be to combine all five previously recognized families into a single family, Membracidae sensu lato; the other option could be to render Cicadellidae monophyletic by excluding Megophthalminae and Ulopinae from Cicadellidae and elevating them to status as separate families.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48692972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why are there so many species of mining bees (Hymenoptera, Andrenidae)? The possible roles of phenology and Wolbachia incompatibility in maintaining species boundaries in the Andrena proxima-complex","authors":"Gabrielle McLaughlin,&nbsp;Morgan Gueuning,&nbsp;David Genoud,&nbsp;Juerg E. Frey,&nbsp;Christophe Praz","doi":"10.1111/syen.12566","DOIUrl":"10.1111/syen.12566","url":null,"abstract":"<p>The factors leading to the extraordinary diversity of the bees of the genus <i>Andrena</i> Fabricius, the second most speciose genus among bees, remain largely unknown. To examine the pattern of diversification in this genus, we investigate species boundaries within the controversial <i>Andrena proxima</i>-complex using a dataset of ultra-conserved elements and various species delimitation analyses (admixture analyses, BPP, DAPC). Our results confirm the presence of three separate species in this species group (<i>Andrena proxima</i> (Kirby), <i>A. ampla</i> Warncke and <i>A. alutacea</i> Stöckhert) and suggest very low levels of interspecific gene flow. <i>Andrena proxima</i> and <i>A. alutacea</i> are regularly found in sympatry, suggesting an advanced stage of speciation. By contrast, <i>A. ampla</i> shows a parapatric distribution with both other species. <i>Andrena alutacea</i> harbours a unique strain of <i>Wolbachia</i> Hertig, absent in the other two species, which are infected by the two same strains of <i>Wolbachia</i>. In addition, the three species have distinct phenologies, with <i>A. proxima</i> flying earlier in the season and <i>A. alutacea</i> significantly later; the phenology of <i>A. ampla</i> is intermediate. Our results suggest that <i>Wolbachia</i>-induced incompatibilities and phenological differences maintain species boundaries in this group. The most advanced stage of speciation is observed in the two species showing putatively incompatible strains of <i>Wolbachia</i> and strongly distinct phenologies, <i>A. alutacea</i> and <i>A. proxima</i>. We propose that the smaller differences in phenological differentiation between <i>A. ampla</i> and both other species may explain the observed pattern of parapatric distribution. We discuss how these factors may underlie the high diversification rate observed in other groups of <i>Andrena</i>, a genus characterized by particularly variable and short flying periods.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/syen.12566","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48076297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic revision, molecular phylogeny and biogeography of the antlion tribe Acanthoplectrini (Neuroptera: Myrmeleontidae: Dendroleontinae), with emphasis on the Oriental lineage","authors":"Yuchen Zheng,&nbsp;Davide Badano,&nbsp;Xingyue Liu","doi":"10.1111/syen.12561","DOIUrl":"10.1111/syen.12561","url":null,"abstract":"<p>The tribe Acanthoplectrini (Myrmeleontidae: Dendroleontinae) includes a group of antlion genera widely distributed across the Australasian and Oriental regions. The intergeneric and interspecific relationships between or within the Australian and Oriental lineages of this tribe as well as their historical biogeography remain largely unexplored. Here, we present a molecular phylogenetic and biogeographic analyses of Acanthoplectrini to infer the diversification history of this tribe, with emphasis on the Oriental lineage. Both the Oriental and Australian lineages are monophyletic and recovered as sister groups. Ancestral area reconstruction suggests that the ancestor of Acanthoplectrini might have been once widely distributed from Indochina to Australia and then split into the Oriental and Australian lineages during the early-Miocene. Our analyses recovered northeastern Indochina and south China as the ancestral range of the Oriental Acanthoplectrini. During the mid-Miocene to the mid-Pliocene, orographic events such as the rising of mountain ranges (including the Himalayas) and the formation of major islands in southeastern Asia triggered several dispersal and vicariance events in the Oriental Acanthoplectrini, driving their speciation. We revise the classification of the Oriental Acanthoplectrini, establishing the new genus <i>Paralayahima</i> <b>gen. n.</b>, which is recovered sister to <i>Layahima</i> Navás. Moreover, we describe four new species of <i>Layahima</i>, <i>Layahima aspoeckorum</i> <b>sp. n.</b>, <i>Layahima monba</i> <b>sp. n.</b>, <i>Layahima lhoba</i> <b>sp. n.</b> and <i>Layahima xinliae</i> <b>sp. n.</b>, and we reinstate two previously synonymized species, <i>Layahima melanocoris</i> (Yang) <b>stat. rev.</b> and <b>comb. n.</b> and <i>Layahima nebulosa</i> Navás <b>stat. rev.</b></p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49378535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Permian parallelisms: Reanalysis of †Tshekardocoleidae sheds light on the earliest evolution of the Coleoptera","authors":"Brendon Elias Boudinot,&nbsp;Evgeny Viktorovich Yan,&nbsp;Jakub Prokop,&nbsp;Xiao-Zhu Luo,&nbsp;Rolf Georg Beutel","doi":"10.1111/syen.12562","DOIUrl":"10.1111/syen.12562","url":null,"abstract":"<p>The Coleoptera provides an excellent example of the value of fossils for understanding the evolutionary patterns of recent lineages. We reevaluate the morphology of the Early Permian †Tshekardocoleidae to test alternative phylogenetic hypotheses relating to the Palaeozoic evolution of the order. We discuss prior interpretations and revise an earlier data matrix. Both Bayesian and parsimony analyses support the monophyly of Coleoptera excluding †Tshekardocoleidae (= Mesocoleoptera), and of Coleoptera excluding †Tshekardocoleidae and †Permocupedidae (= Metacoleoptera). Plesiomorphies preserved in †Tshekardocoleidae are elytra, which rest over the body in a loose tent-like manner, with flat lateral flanges, projecting beyond the abdominal apex, and abdomens that are flexible and nearly cylindrical. Apomorphies of Mesocoleoptera include shortening of the elytra and a closer fit with the flattened and probably more rigid abdomen. A crucial synapomorphy of Metacoleoptera is the tightly sealed subelytral space, which may have been advantageous during the Permian aridification. Taxon exclusion experiments show that †Tshekardocoleidae is crucial for understanding the early evolution of Coleoptera and that its omission strongly affects ancestral state polarities as well as topology, including crown-group taxa. By constraining the relationships of extant taxa to match those supported by phylogenomic analysis, we demonstrate that features shared by Archostemata with Permian stem groups are most reasonably supported as plesiomorphic and that the smooth and simplified body forms of Polyphaga, Adephaga, Myxophaga, and Micromalthidae were derived in parallel. Our study highlights the reciprocal illumination of molecular, morphological, and paleontological data, and paves the way for tip-dating analysis across the order.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/syen.12562","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45586999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A phylogenomic approach to species delimitation in the mango fruit fly (Bactrocera frauenfeldi) complex: A new synonym of an important pest species with variable morphotypes (Diptera: Tephritidae)","authors":"Camiel Doorenweerd,&nbsp;Michael San Jose,&nbsp;Scott Geib,&nbsp;Julian Dupuis,&nbsp;Luc Leblanc,&nbsp;Norman Barr,&nbsp;Elaida Fiegalan,&nbsp;Kimberley Y. Morris,&nbsp;Daniel Rubinoff","doi":"10.1111/syen.12559","DOIUrl":"10.1111/syen.12559","url":null,"abstract":"<p>Species that are dispersed across oceanic islands can have strong population structure due to genetic isolation, which makes it difficult to determine realistic and meaningful species boundaries. This becomes especially problematic when pest species are involved, and can result in undetected new invasions. The mango fruit fly, <i>Bactrocera frauenfeldi</i> (Schiner), is currently considered to be one of five morphologically similar members in a monophyletic species group distributed across Southeast Asia, Australasia, and Oceania, including three major pests. We used a phylogenomic approach with highly multiplexed amplicon sequencing to test species limits and evaluate the relationships among species in the <i>B. frauenfeldi</i> species complex and two closely related species. We obtained sequence data from 196 specimens for 395 nuclear DNA loci, totalling 102 kb, of which 2.2 kb were parsimony informative sites. Based on morphology, biogeography, and phylogenetic analyses, we conclude that there are five distinct species in the complex in our phylogeny. Our results show that the morphological differences between <i>B. frauenfeldi</i> and <i>B. albistrigata</i> (de Meijere) are part of a continuum that cannot be phylogenetically separated into monophyletic groups. We therefore synonymize the names of two major pests: <i>B. albistrigata</i> <b>syn. rev.</b> with <i>B. frauenfeldi</i>, making <i>B. frauenfeldi</i> now recognized as a widespread pest across Australasia and Southeast Asia. We evaluated the use of COI for pest recognition and conclude that it cannot reliably distinguish between six of the seven species we studied, thus new molecular approaches will be necessary for effective management and the prevention of incursions.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48368215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investment in visual system predicted by floral associations in sap beetles (Coleoptera: Nitidulidae)","authors":"Gareth S. Powell,&nbsp;Seth M. Bybee","doi":"10.1111/syen.12558","DOIUrl":"10.1111/syen.12558","url":null,"abstract":"<p>Patterns in morphological variation are a central theme of evolution. Uncovering links between morphological character evolution and natural history, specifically feeding behaviour, is important to understanding biological diversity. Species within the sap beetles (Nitidulidae) exhibit a tremendous diversity of feeding behaviours. This immense diversity of feeding can be seen both between major lineages and very closely related taxa. Feeding behaviour diversity may drive morphological variation in several character systems (e.g., eyes). For example, in a shift from feeding on rotting fruit to flower-visiting (anthophily), selective pressures on the visual system may vary and ultimately lead to differences in eye morphology. We tested for potential morphological shifts in relative eye size among adult beetles. We specifically tested for significant relationships between relative eye size and the following factors flower-visiting and sex. We also tested for the influence of phylogeny on the evolution of relative eye size, implementing tests of trait correlation across a topology. We found greater relative eye size in taxa exhibiting anthophilous behaviour, regardless of phylogenetic relatedness or feeding behaviour of sister taxa. We were unable to recover a relationship between relative eye size and sex. Thus, feeding behaviour is currently the strongest predictor of eye size in sap beetles.</p>","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43942945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delimiting continuity: Comparison of target enrichment and double digest restriction‐site associated DNA sequencing for delineating admixing parapatric Melitaea butterflies","authors":"M. Joshi, M. Espeland, V. Dincă, R. Vilà, M. S. Tahami, Lars Dietz, C. Mayer, S. Martin, L. Dapporto, M. Mutanen","doi":"10.1111/syen.12557","DOIUrl":"https://doi.org/10.1111/syen.12557","url":null,"abstract":"Parapatrically distributed taxa pose a challenge for species delimitation due to the presence of gene flow and inherent arbitrariness of exactly defining the species boundaries in such systems. We tackled the problem of species delimitation in a parapatric species pair of Melitaea butterflies using two popular genomic methods—double digest restriction‐site associated DNA sequencing (ddRAD) and target enrichment. We compared newly generated target enrichment dataset with 1733 loci to the already available ddRAD data from a previous study on the same set of specimens using a suite of phylogenetic, population genetic, and species delimitation methods. We recovered consistent phylogenetic relationships across the datasets, both demonstrating the presence of a genetically distinct Balkan lineage and paraphyly of Melitaea athalia with respect to Melitaea celadussa. Population genetic STRUCTURE analyses supported the presence of two species when using ddRAD data, but three species when using target enrichment, while a Bayes factor delimitation analysis found both two and three species scenarios equally decisive in both datasets. As the results obtained from both methods were largely congruent, we discuss some practical considerations and benefits of target enrichment over RAD sequencing. We conclude that the choice of method of genomic data collection does not influence the results of phylogenetic analyses at alpha taxonomic level, given a sufficient number of loci. Finally, we recommend a solution for delineating species in parapatric scenarios by proposing that parapatric taxa be consistently classified as subspecies or complete species, but not both, to promote taxonomic stability.","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47150622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unearthing underground predators: The head morphology of larvae of the moth lacewing genus Ithone Newman (Neuroptera: Ithonidae) and its functional and phylogenetic implications","authors":"Di Li, F. Friedrich, Kenny Jandausch, H. Pohl, Xingyue Liu, R. Beutel","doi":"10.1111/syen.12556","DOIUrl":"https://doi.org/10.1111/syen.12556","url":null,"abstract":"Moth lacewings (Ithonidae) are a rare group of Neuroptera with an unusual subterranean larval life‐style. We examined external and internal head structures of an older‐instar larva of Ithone Newman with a broad spectrum of techniques. Larval autapomorphies, likely correlated with the subterranean habits, are the compact and shovel‐shaped head, unusually massive mandibular‐maxillary stylets, and a C‐shaped postcephalic body. Other cephalic autapomorphies are the massive X‐shaped tentorium, incurved antennae, and a strongly developed M. verticopharyngalis. The visual organs are distinctly simplified but a single functional stemma on each side of head is retained despite of the subterranean habits. In contrast to previous studies, a well‐developed gular sclerite is present in Ithonidae, possibly a secondary acquisition. A cephalic gland complex and poison channel are present, with an unexpected additional lateral accessory gland and an additional lateral channel. The poison glands and dual channels very clearly indicate that the larvae are predators, contradicting the phytophagous habits formerly postulated. Compared with soil‐inhabiting scarabaeoid beetle larvae, striking differences of head structures are due to different feeding habits and phylogenetic constraints. Morphological similarities like a C‐shaped postcephalic body and strongly developed legs suitable for burrowing in soil are evolutionary parallels associated with the subterranean life‐style in the two non‐related groups. Bayesian phylogenetic analysis was carried out with an updated morphological matrix. The results were compared with a phylogeny based on anchored hybrid enrichment data. The evolutionary transformations of selected characters were evaluated using phylogenies estimated from both datasets.","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48305206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogeny, biogeography and classification of Teletisoptera (Blattaria: Isoptera)","authors":"Menglin Wang, S. Hellemans, J. Šobotník, J. Arora, A. Buček, D. Sillam-Dussès, C. Clitheroe, Tomer Lu, N. Lo, M. Engel, Y. Roisin, T. Evans, T. Bourguignon","doi":"10.1111/syen.12548","DOIUrl":"https://doi.org/10.1111/syen.12548","url":null,"abstract":"Termites are social cockroaches distributed throughout warm temperate and tropical ecosystems. The ancestor of modern termites roamed the earth during the early Cretaceous, suggesting that both vicariance and overseas dispersal may have shaped the distribution of early diverging termites. We investigate the historical biogeography of three early diverging termite families –Stolotermitidae, Hodotermitidae and Archotermopsidae (clade Teletisoptera) – using the nuclear rRNA genes and mitochondrial genomes of 27 samples. Our analyses confirm the monophyly of Teletisoptera, with Stolotermitidae diverging from Hodotermitidae + Archotermopsidae approximately 100 Ma. Although Hodotermitidae are monophyletic, our results demonstrate the paraphyly of Archotermopsidae. Phylogenetic analyses indicate that the timing of divergence among the main lineages of Hodotermitidae + Archotermopsidae are compatible with vicariance. In the Stolotermitidae, however, the common ancestors of modern Porotermes Hagen and Stolotermes Hagen are roughly as old as 20 and 35 Ma, respectively, indicating that the presence of these genera in South America, Africa and Australia involved over‐water dispersals. Overall, our results suggest that early diverging termite lineages acquired their current distribution through a combination of over‐water dispersals and dispersal via land bridges. We clarify the classification by resolving the paraphyly of Archotermopsidae, restricting the family to Archotermopsis Desneux and Zootermopsis Emerson and elevating Hodotermopsinae (Hodotermopsis Holmgren) as Hodotermopsidae (status novum).","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42489569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nuclear copies of mitochondrial DNA as a potential problem for phylogenetic and population genetic studies of Odonata","authors":"Stanislav Ožana, A. Dolný, T. Pánek","doi":"10.1111/syen.12550","DOIUrl":"https://doi.org/10.1111/syen.12550","url":null,"abstract":"The use of mitochondrial markers for taxonomic identification and biodiversity monitoring is not without risks or limitations. Most importantly, the natural transfer of DNA from the mitochondria to the nucleus generates nonfunctional nuclear copies of mitochondrial DNA (NUMTs). Their abundance and size vary significantly among taxa, and NUMTs have been reported to complicate molecular studies based on mitochondrial markers in several insect orders, most prominently in Orthoptera. The significance of this phenomenon in Odonata has not yet been properly addressed. Here, we present a complete mitochondrial genome and a draft nuclear genome of Leucorrhinia albifrons (Odonata: Libellulidae), as well as NUMT and cox1 sequences from the related species Leucorrhinia dubia. We document the presence of NUMTs in the L. albifrons nuclear genome and in nuclear genomes of two other Odonata species available in public databases. Our results show that NUMTs can have a serious impact on barcoding, phylogenetic, population and phylogeographic studies of Odonata, especially when the barcode is located in the cox1 gene, the most frequently used molecular marker for Odonata. We suggest that nad1 should be used alone or in combination with cox1 to minimize unintended confusion with NUMTs. Finally, we present a mitophylogenomic analysis of Odonata and document several cases of misidentified mitochondrial genomes belonging to species different from those indicated in public databases. In conclusion, our findings represent an important step for future metabarcoding studies of Odonata based on mitochondrial DNA markers.","PeriodicalId":22126,"journal":{"name":"Systematic Entomology","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47688149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}