Translation: The University of Toledo Journal of Medical Sciences最新文献

{"title":"Machine Learning Analysis of Identifies Polyunsaturated Fatty Acid Metabolites Predictive of Adverse Outcomes In Heart Failure with Preserved Ejection Fraction Patients","authors":"Dhilhani Faleel, Ahmed Elzanaty, Vaishnavi Aradhyula, Rohit Vyas, Prabhatchandra R. Dube, Steven T. Haller, Rajesh Gupta, David J. Kennedy, Samer J. Khouri","doi":"10.46570/utjms.vol11-2023-951","DOIUrl":"https://doi.org/10.46570/utjms.vol11-2023-951","url":null,"abstract":"which molecules were associated with future risk of either all cause death or a combined adverse outcome of death or rehospitalization in the setting of HFpEF. Results: In patients with HFpEF, increased levels of 9(10)-Epome, 15(R)-PGE1, 17-oxoRvD1, TXB3, RvD3, 5(S),15(S)-DiHETE, and 11dh-2,3-dinor TXB2 at baseline were predictive of all cause mortality (all p<0.05). Increased baseline levels of 8-oxoRvD1, MaR1(n-3DPA), PGE3, and 5,6-DiHETrE were predictive of the combined adverse outcome of death or rehospitalization (all p<0.05). Conclusion: These findings support the hypothesis that distinct PUFA metabolites play a significant role in mediating cardiovascular disease in HFpEF. Our study introduces a novel lipidomics framework for the diagnostic and prognostic assessment of cardiovascular risk in HFpEF patients.","PeriodicalId":220681,"journal":{"name":"Translation: The University of Toledo Journal of Medical Sciences","volume":"5 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139001014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Rare Case of Localized Colonic Amyloidosis Identified During a Screening Colonoscopy","authors":"S. Ghazaleh, Chmsalddin Alkhas, Ali Heif, M. Heif","doi":"10.46570/utjms.vol11-2023-923","DOIUrl":"https://doi.org/10.46570/utjms.vol11-2023-923","url":null,"abstract":"","PeriodicalId":220681,"journal":{"name":"Translation: The University of Toledo Journal of Medical Sciences","volume":"11 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138996340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protocol Development for Yielding High Quality DNA and RNA from Archived Formalin-Fixed Paraffin Embedded Tissues","authors":"Sara Kazmi, Bella Khatib-Shahidi, John Najjar, Anish Sharma, Caitlin Murphy, Humza Bashir, Benjamin French, Apurva Lad, David Kennedy, Steven Haller","doi":"10.46570/utjms.vol11-2023-939","DOIUrl":"https://doi.org/10.46570/utjms.vol11-2023-939","url":null,"abstract":"Introduction: While genetic analysis of archived formalin-fixed paraffin embedded (FFPE) tissue specimens would be a significant research resource, extraction of high-quality DNA and RNA from these specimens is a significant challenge. Storage duration, tissue handling and tissue preservation processes as well as their interactions with the nucleic acids could influence the quality and integrity of the DNA or RNA required for genetic analysis. \u0000Objectives: The goal of this study was to establish a protocol to extract high quality DNA and RNA from the FFPE tissues for further use in quantitative PCR analysis. \u0000Methods: For the purposes of this study, human FFPE biopsy tissues from lung, liver, colon and kidney were obtained from a single center biorepository. GeneJET Genomic DNA Purification Kit (Catalog # K0722) obtained from Thermo Fisher Scientific and RecoverAll Total Nucleic Acid Isolation Kit obtained from Life Technologies were modified and used for extraction of DNA and RNA, respectively. Briefly, modifications involved extending reagent incubation times, increasing sample volumes and wash steps, and increased final nucleic acids recovery and concentration steps. Eight sections around 8-10 ?m thick were microtomed for each tissue sample and used for extraction. The purity of the nucleic acids obtained was verified using Nanodrop Spectrophotometer. \u0000Results: The average DNA yield from eight sections for each of the tissues was 270±184 ng/?l and for RNA was 296±188 ng/?l. Nucleic acid quality was assessed by measuring the 260nm/280nm absorbance ratio for protein contamination as well as the 260nm/230nm absorbance ratio for salt contamination.  Both were found to be within acceptable ranges. RNA was reverse transcribed to cDNA and qPCR was successfully performed on both DNA and cDNA samples. \u0000Conclusions: These results indicate that protocols using the silica-based membrane technology can yield high quality DNA and RNA that can be successfully used for downstream genetic analysis.","PeriodicalId":220681,"journal":{"name":"Translation: The University of Toledo Journal of Medical Sciences","volume":"8 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138996403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histoplasmosis of the left wrist in an immunosuppressed host with myasthenia gravis","authors":"Dr. Lance D. Dworkin, Noor Abdulhameed, Omar Abdul-Aziz, Meghan Sawyer, C. Hollingshead, MD Salman Arif","doi":"10.46570/utjms.vol11-2023-828","DOIUrl":"https://doi.org/10.46570/utjms.vol11-2023-828","url":null,"abstract":"","PeriodicalId":220681,"journal":{"name":"Translation: The University of Toledo Journal of Medical Sciences","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138997605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycobacterium goodii Associated with Breast Tissue Expanders","authors":"Ambika Sood, Victoria Starnes, Dr. Caitlyn Hollingshead, David Chu","doi":"10.46570/utjms.vol11-2023-824","DOIUrl":"https://doi.org/10.46570/utjms.vol11-2023-824","url":null,"abstract":"","PeriodicalId":220681,"journal":{"name":"Translation: The University of Toledo Journal of Medical Sciences","volume":"13 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138997738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Newly Diagnosed Severe AIDS with Recovery of CMV Retinitis with concurrent Cryptococcal Meningitis","authors":"Zachary Holtzapple, Ayman Salih, Cassidy Eby, Mitchell Salke, C. Hollingshead","doi":"10.46570/utjms.vol11-2023-846","DOIUrl":"https://doi.org/10.46570/utjms.vol11-2023-846","url":null,"abstract":"","PeriodicalId":220681,"journal":{"name":"Translation: The University of Toledo Journal of Medical Sciences","volume":"5 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138998008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyarteritis Nodosa Presenting With Abdominal Pain and Mesenteric Stenosis","authors":"Jerrin George, Avish Persaud, MD Nezam Altorok, MD Nathaniel Gilbert","doi":"10.46570/utjms.vol11-2023-890","DOIUrl":"https://doi.org/10.46570/utjms.vol11-2023-890","url":null,"abstract":"","PeriodicalId":220681,"journal":{"name":"Translation: The University of Toledo Journal of Medical Sciences","volume":"37 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138998674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of palindromic rheumatism and literature review","authors":"Dharmindra Dulal, B. Kahaleh","doi":"10.46570/utjms.vol11-2023-880","DOIUrl":"https://doi.org/10.46570/utjms.vol11-2023-880","url":null,"abstract":"","PeriodicalId":220681,"journal":{"name":"Translation: The University of Toledo Journal of Medical Sciences","volume":"117 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138999429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Evaluation of a Pharmacist-Led Multidisciplinary Quality Committee in a General Internal Medicine Outpatient Clinic","authors":"Marilee Clemons, Sarah Aldrich Renner, William Barnett","doi":"10.46570/utjms.vol11-2023-821","DOIUrl":"https://doi.org/10.46570/utjms.vol11-2023-821","url":null,"abstract":"","PeriodicalId":220681,"journal":{"name":"Translation: The University of Toledo Journal of Medical Sciences","volume":"2 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139000205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple Sclerosis anti-CD20 (Ocrelizumab) therapy inducing hypogammaglobulinemia","authors":"Nora Abdul-Aziz, Noor Abdulhameed, A. Marellapudi, Haroon Shah","doi":"10.46570/utjms.vol11-2023-836","DOIUrl":"https://doi.org/10.46570/utjms.vol11-2023-836","url":null,"abstract":"","PeriodicalId":220681,"journal":{"name":"Translation: The University of Toledo Journal of Medical Sciences","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138996013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}