{"title":"Monitoring cell adhesion and characterizing cell viscoelasticity by using thickness shear mode (TSM) resonate sensor","authors":"Fang Li, Qing-Ming Wang, J.H.-C. Wang","doi":"10.1109/FREQ.2008.4623020","DOIUrl":"https://doi.org/10.1109/FREQ.2008.4623020","url":null,"abstract":"Cell adhesion and mechanical properties are critical to physiological and pathological processes. Regulation of cell adhesion states is involved in tissue remodeling during morphogenesis and wound healing, cellular metaplasia, cell proliferation and tumor cell metastasis. Cell mechanical properties play an essential role in cell deformation under mechanical forces and other cell functions such as locomotion and cytokinesis. Because of the dominance of the cytoskeleton in cell structure and properties, measurement of the cell viscoelastic properties provides an effective approach to look into cytoskeleton, which plays an important role in cell growth, gene expression, protein synthesis, differentiation, migration and apoptosis. In this study, a functional biosensor system, which consists of acoustic wave biosensor array, cell culture, a mini-incubator, an impedance analyzer and a computer, was established to monitor cell adhesion and viscoelastic properties under controlled biological conditions. A multilayer acoustic wave sensor model that includes the thickness shear mode quartz resonator substrate, a cell-substrate interfacial layer and a cell layer in cell culture medium was constructed based on the state of cell adhesion to the substrate, which can be applied to predict the relationship between the resonant frequency and resistance change of the biosensor and physical properties of the interfacial layer and the cell layer. Experimentally, the dynamic processes of cell adhesion as function of cell seeding densities have been investigated. Using the theoretical model, the viscoelastic properties of cell layer are extracted by fitting the theoretical values of the resonator admittance near resonance with the measured spectrum. The results agree very well with the data obtained by other techniques.","PeriodicalId":220442,"journal":{"name":"2008 IEEE International Frequency Control Symposium","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126282603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Malocha, J. Pavlina, D. Gallagher, N. Kozlovski, B. Fisher, N. Saldanha, D. Puccio
{"title":"Orthogonal frequency coded SAW sensors and RFID design principles","authors":"D. Malocha, J. Pavlina, D. Gallagher, N. Kozlovski, B. Fisher, N. Saldanha, D. Puccio","doi":"10.1109/FREQ.2008.4623004","DOIUrl":"https://doi.org/10.1109/FREQ.2008.4623004","url":null,"abstract":"Orthogonal frequency coded (OFC) SAW reflectors and transducers have been recently introduced for use in communication, sensor and RFID tag applications.[1,2] The OFC SAW technology approach has been funded by NASA for possible inclusion in ground, space flight and space exploration sensor applications. In general, SAW technology has advantages over possible competing technologies: passive, wireless, radiation hard, operation from cryogenic to furnace temperature ranges, small, rugged, variable frequency and bandwidth operation, encoding and commercially available. SAW sensor embodiments can provide onboard device sensor integration, or can provide integration with an external sensor that uses the SAW device for encoding the sensor information and transmission to the receiver. SAW OFC device technology can provide RFID tags and sensors with low loss, large operating temperatures and a multi-use sensor platform. This paper will discuss the key parameters for OFC device design, which include reflector and transducer design, coding diversity approaches, and insertion loss considerations. Examples of several OFC device sensors and RFID tags will be presented to show the current state-of-the-art performance for several NASA applications, as well as projections for future sensor and RFID tag platform performance.","PeriodicalId":220442,"journal":{"name":"2008 IEEE International Frequency Control Symposium","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125927554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Brandl, Stefan Dipl.-Ing. Schuster, S. Scheiblhofer, A. Stelzer
{"title":"A new anti-collision method for SAW tags using linear block codes","authors":"M. Brandl, Stefan Dipl.-Ing. Schuster, S. Scheiblhofer, A. Stelzer","doi":"10.1109/FREQ.2008.4623005","DOIUrl":"https://doi.org/10.1109/FREQ.2008.4623005","url":null,"abstract":"In this paper a new separation/anti-collision method for simultaneous interrogation of multiple surface acoustic wave identification (SAW-ID) tags is presented. The tags are encoded by means of a linear block code . This approach allows to retrieve lost data caused by destructive superposition of the response signals of multiple tags. Another advantage of this principle is that the IDs of the tags result directly without scanning all possible combinations of tags. A typical application is multi-object tagging in an industrial environment.","PeriodicalId":220442,"journal":{"name":"2008 IEEE International Frequency Control Symposium","volume":"225 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122363294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Lumped element equivalent circuit for shear piezoelectric cylindrical resonators","authors":"P. Kielczynski, M. Szalewski","doi":"10.1109/FREQ.2008.4623069","DOIUrl":"https://doi.org/10.1109/FREQ.2008.4623069","url":null,"abstract":"The lumped element equivalent circuit for piezoelectric cylindrical resonators polarized axially and vibrating in a shear mode in the vicinity of their resonance is presented. Closed analytical expressions for the equivalent-circuit parameters L1, C1, R1 and C0 for free piezoelectric cylindrical resonator were established. All element values obtained are functions of material and geometrical properties of the resonator. Moreover, using the perturbation theory, the analytical formulas for additional elements L2, C2, R2 contributed by the viscoelastic loading were established. The results obtained can be used in the design and development of the piezoelectric cylindrical resonators, sensors and filters.","PeriodicalId":220442,"journal":{"name":"2008 IEEE International Frequency Control Symposium","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128263872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Perrin, F. Esnault, D. Holleville, S. Guérandel, N. Dimarcq, V. Ligeret, J. Delporte
{"title":"A compact optical bench for laser cooling","authors":"S. Perrin, F. Esnault, D. Holleville, S. Guérandel, N. Dimarcq, V. Ligeret, J. Delporte","doi":"10.1109/FREQ.2008.4622970","DOIUrl":"https://doi.org/10.1109/FREQ.2008.4622970","url":null,"abstract":"We present the architecture of a A4 size optical bench developed for the compact cold atom clock HORACE. This proceeding focuses on two original sub systems as compact extended cavity laser diode with optical isolator places inside the laser cavity, and very compact saturated absorption systems.","PeriodicalId":220442,"journal":{"name":"2008 IEEE International Frequency Control Symposium","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128529963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Stress control in AlN and Mo films for electro-acoustic devices","authors":"V. Felmetsger, P. Laptev","doi":"10.1109/FREQ.2008.4623076","DOIUrl":"https://doi.org/10.1109/FREQ.2008.4623076","url":null,"abstract":"Piezoelectric AlN films with strong (002) crystal orientation are widely used in various resonator-based applications such as BAW and FBAR filters, oscillators and resonating sensors. Low intrinsic stress is one of the important requirements for the thin film stacks employed in electro-acoustic devices. In this paper, we describe technical and technological solutions enabling effective stress control in piezoelectric AlN films deposited with an ac (40 kHz) reactive sputtering process by a dual cathode S-Gun magnetron, and in Mo electrodes deposited by a DC powered S-Gun. In the AC powered S-Gun, a special stress adjustment unit reduces compressive stress in the AlN films by controllably suppressing the flux of charged particles to the substrate by means of discharge current redistribution between the targets and the internal shields of the magnetron. Tensile stress in the AlN and Mo films is effectively reduced by performing the thin film depositions with added rf substrate bias. To avoid texture deterioration in the Mo films deposited with RF biasing, a two-step deposition process has been developed ensuring formation of superior crystal orientation as well as near-zero or, if required, compressive stress in the Mo films. Formation of a well-textured Mo bottom electrode enables growth of highly c-axis oriented AlN films.","PeriodicalId":220442,"journal":{"name":"2008 IEEE International Frequency Control Symposium","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130567821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optical lattice clocks with non-interacting bosons and fermions","authors":"H. Katori, M. Takamoto, T. Akatsuka, H. Hachisu","doi":"10.2184/LSJ.36.1004","DOIUrl":"https://doi.org/10.2184/LSJ.36.1004","url":null,"abstract":"We discuss configurations of optical lattice clocks from the viewpoint of lattice geometries and quantum statistics of interrogated atoms. This leads to two promising schemes; one-dimensional lattice loaded with spin-polarized fermions and three-dimensional lattice loaded with bosons. These two optical lattice clocks based on fermionic 87Sr and bosonic 88Sr were demonstrated by measuring the frequency stability as well as their isotope shift. In addition, we address our ongoing experimental challenges for optical lattice clocks.","PeriodicalId":220442,"journal":{"name":"2008 IEEE International Frequency Control Symposium","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131464992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A new method to increase loaded Q of extremely low phase noise SAW oscillator","authors":"T. Imaike, Y. Sakuta, Y. Sekine","doi":"10.1109/FREQ.2008.4622983","DOIUrl":"https://doi.org/10.1109/FREQ.2008.4622983","url":null,"abstract":"We have previously proposed a circuit configuration of SAW oscillator for extremely low phase noise, the name of which is Fr oscillator. The Fr oscillator has been able to realize extremely low phase noise floor level in calculation; however, the loaded Q is lower than Colpitts type oscillator. Then we propose new method to increase loaded Q. Using this method, we show several characteristics of the loaded Q. Based on the results, we estimate SSB phase noise of the Fr oscillator. As a result, the phase noise level is improved about 15 dB in the neighborhood of carrier frequency if the input/output impedance of the frequency selective circuit is reduced. Furthermore, we make a couple of Fr oscillators using crystal resonator. The measured phase noise characteristics are corresponding to the calculated value well. It is clear that this method is effective for improving phase noise of near the carrier frequency.","PeriodicalId":220442,"journal":{"name":"2008 IEEE International Frequency Control Symposium","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134519652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multi-mode excitation of electromagnetic-acoustic resonant sensor arrays","authors":"F. Lucklum, B. Jakoby","doi":"10.1109/FREQ.2008.4622955","DOIUrl":"https://doi.org/10.1109/FREQ.2008.4622955","url":null,"abstract":"Electromagnetic excitation of acoustic sensors as an alternative to piezoelectric and capacitive excitation allows for non-contact measurements and the generation of multiple modes of vibration. It is possible to utilize the same excitation setup to excite flexural, face shear, and thickness shear modes with no changes to the resonator itself. In this contribution we propose a sensor array setup to take advantage of this unique property. We will demonstrate the possibility of simultaneously exciting similar and different vibration modes in the different array elements. This allows for increased redundancy in experimental measurement and the simultaneous determination of multiple properties, e.g. viscosity, density, liquid volume in a single measurement. As a proof of concept, we have fabricated a metal sensor array on 100 mum thick brass sheets. We will present experimental results and a comparison of sensor arrays to single elements for measurements in liquids at different densities and viscosities.","PeriodicalId":220442,"journal":{"name":"2008 IEEE International Frequency Control Symposium","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132661455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An analysis of propagation of surface acoustic waves in rotating piezoelectric plates","authors":"Ji Wang, R. Wu, Jianke Du, Dejin Huang","doi":"10.1109/FREQ.2008.4622977","DOIUrl":"https://doi.org/10.1109/FREQ.2008.4622977","url":null,"abstract":"As an important application of acoustic wave sensors, gyroscopes for rotation sensing purpose can also utilize surface acoustic waves in piezoelectric substrates. This requires an analysis of surface acoustic waves propagating in finite piezoelectric solids to aid the design process in parameter selection and optimization. Earlier studies have investigated the effect of rotation in semi-infinite piezoelectric solids, but result like this can only be considered as an approximation to actual gyroscopes with finite substrates. In this study, we consider the substrate as an infinite piezoelectric plate with finite thickness, thus enabling us to calculate the surface acoustic wave velocity in a rotating plate. It is known that there is only one velocity in a semi-infinite solid, but there will be two velocities if we study surface waves in an infinite plate. It is also found that both velocities are changed by the rotation. The numerical results on AT- and ST-cut quartz crystal show that both velocity branches have been changed and the two velocities can be clearly observed if the thickness of plate is less than three wavelengths. If the substrate is to be considered as a semi-infinite, the plate thickness has to be larger than three wavelengths. In this case, the effect of rotation on the velocity still exists, but the two modes essentially merged into the Rayleigh wave velocity. The velocity shifts due to rotation is different from the semi-infinite substrate, offering improved references and guidance in the substrate and material selection for rotation piezoelectric sensors. The extension of this study can be done with the application of two-dimensional theory for surface acoustic waves in finite plates.","PeriodicalId":220442,"journal":{"name":"2008 IEEE International Frequency Control Symposium","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132797267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}