Steroids最新文献_第2页

The effect of resistance training and nandrolone decanoate administration on cardiac tissue in mice 抗阻训练与癸酸诺龙对小鼠心脏组织的影响。

IF 2.1 4区医学

Steroids Pub Date : 2025-02-01 DOI: 10.1016/j.steroids.2024.109559

Yechiel Atias, Tavor Ben-Zeev, Chagai Levi, Lior Binman, Jay R. Hoffman

{"title":"The effect of resistance training and nandrolone decanoate administration on cardiac tissue in mice","authors":"Yechiel Atias, Tavor Ben-Zeev, Chagai Levi, Lior Binman, Jay R. Hoffman","doi":"10.1016/j.steroids.2024.109559","DOIUrl":"10.1016/j.steroids.2024.109559","url":null,"abstract":"<div><div><strong>Purpose:</strong> This study examined the effect of resistance training (RT) by itself and in combination with supraphysiological administration of nandrolone decanoate (ND) on the inflammatory, apoptotic, and oxidative stress response in cardiac tissue. The effect of the training and androgen intervention on adiponectin expression, a potential cardio protectant was also examined. <strong>Methods:</strong> Forty male C57Bl/6J mice, 3 months of age were randomized into four groups (n = 10 per group). Two groups of animals performed a 3-day per week RT program for 7-weeks, while the other two groups remained sedentary (SED). The RT and SED animals were further randomized into an androgen group (RTA and SEDA, respectively) or a sham group (RTS and SEDS, respectively). Animals in the RTA and SEDA groups received 38-mg·kg<sup>−1</sup> injected once per week. Mice from RTS and SEDS received sham injections. <strong>Results:</strong> Main effects for group indicated that RT resulted in significant elevations in NFκβ (p < 0.001), glutamine peroxidase (GPX) (p = 0.007) and adiponectin (p < 0.001). Main effects for treatment indicated that ND administration resulted in greater elevations in NFκβ (p = 0.01) and TNF-α (p = 0.017). In addition, TNF-α expression was greater in RTA compared to RETS (p = 0.006) and the adiponectin response in RTA was greater (p’s < 0.05) than all other groups. A significant correlation was noted between average training volume during the RT program and GPX expression (r = 0.716, p < 0.001). <strong>Conclusion:</strong> Results indicate that RT and ND administration can increase markers of apoptosis and inflammation. Elevations in adiponectin expression suggest that it may act as a compensatory mechanism supporting cardiovascular health.</div></div>","PeriodicalId":21997,"journal":{"name":"Steroids","volume":"214 ","pages":"Article 109559"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved synthesis and characterization of bile acid esters: Organogelation and supramolecular properties 胆汁酸酯的改进合成和表征：有机凝胶化和超分子性质。

IF 2.1 4区医学

Steroids Pub Date : 2025-02-01 DOI: 10.1016/j.steroids.2025.109560

Jair García-Méndez , Adolfo López-Torres , María A. Fernández-Herrera

引用次数: 0

Novel compound heterozygous CYP17A1 mutations identified in a family with two siblings affected by 17α-hydroxylase/17,20-lyase deficiency

IF 2.1 4区医学

Steroids Pub Date : 2025-02-01 DOI: 10.1016/j.steroids.2025.109562

Rongbo He , Lin Xu , Nan Yang , Shaoshi Zhu , Hongqi Fan , Jing Zou , Rourou Chen , Li Qian , Yu Liu

{"title":"Novel compound heterozygous CYP17A1 mutations identified in a family with two siblings affected by 17α-hydroxylase/17,20-lyase deficiency","authors":"Rongbo He , Lin Xu , Nan Yang , Shaoshi Zhu , Hongqi Fan , Jing Zou , Rourou Chen , Li Qian , Yu Liu","doi":"10.1016/j.steroids.2025.109562","DOIUrl":"10.1016/j.steroids.2025.109562","url":null,"abstract":"<div><h3>Background</h3><div>17α-Hydroxylase/17,20-lyase deficiency (17OHD) is a rare form of congenital adrenal hyperplasia (CAH), caused by mutations in the <em>CYP17A1</em> gene. It typically manifests clinically as variable degree of hypertension, hypokalemia, and disorders of sexual development (DSD), which can include abnormal sexual differentiation in males and sexual infantilism in females. Over 100 mutations in <em>CYP17A1</em> have been identified, with most cases involving missense mutations or small deletions.</div></div><div><h3>Method</h3><div>This study examined the clinical features, biochemical profiles, and genetic background of two 46, XY siblings from the same family, both diagnosed with 17OHD—a scenario that is uncommonly seen in clinical practice. We performed a genetic analysis of the <em>CYP17A1</em> gene in two generations of the family to confirm the diagnosis.</div></div><div><h3>Results</h3><div>Both patients are phenotypically female, presenting with hypertension, hypokalemia, primary amenorrhea, and absent secondary sexual characteristics. Genetic analysis revealed two novel compound heterozygous mutations in the <em>CYP17A1</em> gene: R45WfsTer5 (a frameshift mutation) and L361P (a missense mutation). Neither variant is reported in the ClinVar database, and the frameshift mutation (R45WfsTer5) is newly identified.</div></div><div><h3>Conclusions</h3><div>The discovery of these two novel <em>CYP17A1</em> mutations expands the genetic spectrum of 17OHD and provides new insights into the genetic underpinnings of the disease. Personalized treatment plans are necessary, and the choice of glucocorticoids with stronger sodium retention effects may be required to manage hypertension and electrolyte imbalances in select patients.</div></div>","PeriodicalId":21997,"journal":{"name":"Steroids","volume":"214 ","pages":"Article 109562"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of novel α-spinasterol derivatives and their inhibitory effects on CCL17 and CCL22 chemokine expression 新型α-spinasterol衍生物的合成及其对CCL17和CCL22趋化因子表达的抑制作用。

IF 2.1 4区医学

Steroids Pub Date : 2025-02-01 DOI: 10.1016/j.steroids.2024.109553

Hyejin Moon , Hongjoon Yoon , Hana Jung, Tae Hoon Lee, Hakwon Kim

{"title":"Synthesis of novel α-spinasterol derivatives and their inhibitory effects on CCL17 and CCL22 chemokine expression","authors":"Hyejin Moon , Hongjoon Yoon , Hana Jung, Tae Hoon Lee, Hakwon Kim","doi":"10.1016/j.steroids.2024.109553","DOIUrl":"10.1016/j.steroids.2024.109553","url":null,"abstract":"<div><div>Natural α-spinasterol is well known for its various biological activities. In this study, we investigated the anti-inflammatory effects of newly synthesized α-spinasterol derivatives by tracking the expression of CCL17 and CCL22 chemokines, which serve as biomarkers for immune cell trafficking in skin inflammation. Initially, the 3-epimer of α-spinasterol, which results from inversion of stereochemistry at the C-3 position of α-spinasterol, was synthesized using the Mitsunobu reaction. Subsequently, new compounds were synthesized by introducing azido, amino, and amide groups at the C-3 position of α-spinasterol or 3-<em>epi</em>-α-spinasterol. The anti-inflammatory activity of these compounds was evaluated by examining their inhibitory effects on the mRNA expression of CCL17 and CCL22. Among these derivatives, 3α-<strong>8</strong>, 3α-<strong>12b</strong>, and 3α-<strong>12c</strong> exhibited potential anti-inflammatory activity <em>in vitro</em>, compared to α-spinasterol. Furthermore, compound 3α-<strong>8</strong> showed even greater activity than 3α-<strong>12b</strong> and 3α-<strong>12c</strong>, underscoring its potential as a highly effective agent. These results suggest that the newly synthesized α-spinasterol derivatives hold promise as candidates for skin inflammation therapeutics.</div></div>","PeriodicalId":21997,"journal":{"name":"Steroids","volume":"214 ","pages":"Article 109553"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allosteric communications between domains of nuclear receptors 核受体区域间的变构通信。

IF 2.1 4区医学

Steroids Pub Date : 2025-02-01 DOI: 10.1016/j.steroids.2024.109551

Fraydoon Rastinejad

{"title":"Allosteric communications between domains of nuclear receptors","authors":"Fraydoon Rastinejad","doi":"10.1016/j.steroids.2024.109551","DOIUrl":"10.1016/j.steroids.2024.109551","url":null,"abstract":"<div><div>Nuclear receptors (NRs) regulate gene expression in response to hormonal signals, influencing diverse physiological processes and diseases. Structural and dynamics investigations based on X-ray crystallography, cryo-electron microscopy (cryo-EM), hydrogen–deuterium exchange mass spectrometry, and molecular dynamics simulations, have significantly deepened our understanding of the conformational states, dynamics, and interdomain interactions of multi-domain NRs. Structural studies have examined heterodimeric complexes such as peroxisome proliferator-activated receptor gamma (PPAR-γ) with retinoid X receptor alpha (RXRα), liver X receptor beta (LXRβ) with RXRα, and retinoic acid receptor beta (RARβ) with RXRα, as well as homodimers like hepatic nuclear factor 4 alpha (HNF-4α), androgen receptor (AR), and glucocorticoid receptor (GR). These investigations highlight critical allosteric communication between ligand-binding domains (LBDs) and DNA-binding domains (DBDs), emphasizing the roles of flexible hinge regions and <em>N</em>-terminal segments in adapting to diverse DNA configurations. Both non-steroid receptor heterodimers and homodimers exhibit robust interdomain connections that mediate allosteric signaling. For instance, AR demonstrates three distinct conformational states that underscore its dynamic behavior, while GR exhibits unique ligand-dependent domain interactions shaping receptor signaling. The collective findings so far suggest a conserved mechanism of cross-domain communication across the NR family. Supported by complementary biophysical, spectroscopic, mutagenesis, and computational studies, this body of research has elucidated the nature of domain-domain interfaces and their pivotal roles in regulating the transcriptional activity of steroid and non-steroid receptors.</div></div>","PeriodicalId":21997,"journal":{"name":"Steroids","volume":"214 ","pages":"Article 109551"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Patterns of corticosterone exposure affect the subcellular localisation of mineralocorticoid and glucocorticoid receptor complexes and gene expression 皮质酮暴露模式会影响矿质皮质激素和糖皮质激素受体复合物的亚细胞定位和基因表达。

IF 2.1 4区医学

Steroids Pub Date : 2025-02-01 DOI: 10.1016/j.steroids.2024.109524

Susana N. Paul , Anna De Visser , Federica Motta , Caroline A. Rivers , John R. Pooley , Stafford L. Lightman , Onno C. Meijer

{"title":"Patterns of corticosterone exposure affect the subcellular localisation of mineralocorticoid and glucocorticoid receptor complexes and gene expression","authors":"Susana N. Paul , Anna De Visser , Federica Motta , Caroline A. Rivers , John R. Pooley , Stafford L. Lightman , Onno C. Meijer","doi":"10.1016/j.steroids.2024.109524","DOIUrl":"10.1016/j.steroids.2024.109524","url":null,"abstract":"<div><div>Mineralocorticoid (MR) and glucocorticoid receptors (GR) act as transcription factors and major mediators of glucocorticoid signalling, with pivotal roles in regulating the stress response and hormonal signalling, mood, cognition and memory. The MR and GR share many target genes, have a high degree of homology in their DNA binding (DBD) and ligand binding domain (LBD) but differ considerably in the <em>N</em>-terminal domain (NTD). Using Proximity Ligation Assay (PLA) we quantitatively assessed MR-GR complex subcellular localisation and transcriptional regulation in murine neuroblastoma (N2A) cells stimulated by constant or pulsatile corticosterone (CORT) patterns. We observe that continuous receptor activation by CORT caused localisation at the periphery of the cell nucleus. Truncation of the receptor Ligand Binding Domain (LBD) led to a stronger localisation of MR-GR complexes at the periphery of the cell nuclei. This was also observed for GR immunofluorescence (IF), while in cells expressing only MR or GR the mRNA response to pulsatile hormone treatment was substantially attenuated. However, there was no clearcut correlation between the spatial distribution of MR-GR complexes and the mRNA levels of target genes. Overall, our findings suggest that longer presence in the cell nucleus favors more peripheral nuclear localisation.</div></div>","PeriodicalId":21997,"journal":{"name":"Steroids","volume":"214 ","pages":"Article 109524"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of estrogen on myocardial ischemia–reperfusion injury in male and female rats and related mechanism

IF 2.1 4区医学

Steroids Pub Date : 2025-02-01 DOI: 10.1016/j.steroids.2025.109561

Sichong Chen , Lijuan Yang , Jiayao Xue , Xinmiao Tian, Huiyuan Hu, Qinghua Gao, Rui Feng, Liying Hao

{"title":"Effect of estrogen on myocardial ischemia–reperfusion injury in male and female rats and related mechanism","authors":"Sichong Chen , Lijuan Yang , Jiayao Xue , Xinmiao Tian, Huiyuan Hu, Qinghua Gao, Rui Feng, Liying Hao","doi":"10.1016/j.steroids.2025.109561","DOIUrl":"10.1016/j.steroids.2025.109561","url":null,"abstract":"<div><div>Due to the difference of estrogen levels in different phases of estrous cycle, it is necessary to exclude the influence of endogenous estrogen when studying the cardiovascular effects of estrogen and its analogues. In this study, the ischemia/reperfusion (I/R) injury of isolated heart were investigated in female rats during different phases of estrous cycle with male rats as comparison. The results indicated that the estrogen content in blood of rats during metestrus and diestrus (MD) was lower than those during proestrus and estrous (PE). 17β-Estradiol (E2) at 10<sup>−8</sup> M did not show significant effects on I/R injury in male rats and female rats during PE. However, E2 exerted an obviously protective effects against I/R injury on heart rate (HR), lactate dehydrogenase (LDH) and creatine kinase (CK) release in female rats during MD. Furthermore, E2 relieved I/R injury in female rats during MD by decreasing the infarct size and the expression level of p-CaMKII. The results suggested estrous cycles may influence on the extent of cardiac I/R injury due to the regulation of E2 on CaMKII expression level, providing an idea that the estrus cycle should be considered for animal model preparation and toxicity studies in estrogen and environmental hormone research.</div></div>","PeriodicalId":21997,"journal":{"name":"Steroids","volume":"214 ","pages":"Article 109561"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143053643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nandrolone decanoate induces heart injury via oxidative damage and mitochondrial apoptotic pathway by regulation of TLR4/NF-κB/NLRP3 axis in male rats: The rescue effect of N-acetylcysteine

IF 2.1 4区医学

Steroids Pub Date : 2025-02-01 DOI: 10.1016/j.steroids.2025.109563

Haniyeh Amini , Alireza Shirpoor , Roya Naderi

{"title":"Nandrolone decanoate induces heart injury via oxidative damage and mitochondrial apoptotic pathway by regulation of TLR4/NF-κB/NLRP3 axis in male rats: The rescue effect of N-acetylcysteine","authors":"Haniyeh Amini , Alireza Shirpoor , Roya Naderi","doi":"10.1016/j.steroids.2025.109563","DOIUrl":"10.1016/j.steroids.2025.109563","url":null,"abstract":"<div><div>Myocardial apoptosis is a leading cause of damage in cardiac tissues of nandrolone (ND) treatment. However, its molecular mechanism is not fully understood. This study aims to investigate the effect of ND with or without N −acetylcysteine (NAC) treatment on oxidative damage and TLR4/NF-κB /NLRP3 signaling pathway in the heart of male rats. Eighteen male Wistar rats with a weight range of 220 ± 10 g were selected. They were divided into three groups (n = 6): control (C) group, ND group, NAC + ND group. After six weeks of treatment, the TUNEL staining indicated that ND increased the number of apoptotic cells in the hearts of male rats. The molecular analysis demonstrated that ND exposure resulted in increased protein levels of cytochrome <em>c</em>, c-Caspase-3/p-Caspase-3 ratio, p53, TLR4, NF-κB, NLRP3, and 8-OHdG with a concomitant up-regulation of LDH and CK-MB enzymes activity in the heart tissue compared to the C group. Our findings suggested that ND can cause damage to heart tissue via induction of DNA damage, apoptosis, and probably TLR4/NF-κB/NLRP3 signaling pathway plays a crucial role in this process. It also demonstrates that these negative effects of ND can be reduced by using NAC treatment as an antioxidant and anti-inflammatory agent.</div></div>","PeriodicalId":21997,"journal":{"name":"Steroids","volume":"214 ","pages":"Article 109563"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The advancement of structure, bioactivity, mechanism, and synthesis of bufotalin 丁福他林的结构、生物活性、机理及合成研究进展。

IF 2.1 4区医学

Steroids Pub Date : 2025-02-01 DOI: 10.1016/j.steroids.2024.109555

Nuo Chen , Yunqiang Wu , Huamao Wei , Shuai Zhi , Liwei Liu

引用次数: 0

Relative fat mass (RFM) is linked to testosterone deficiency in adult males 相对脂肪量（RFM）与成年男性睾酮缺乏有关。

IF 2.1 4区医学

Steroids Pub Date : 2025-02-01 DOI: 10.1016/j.steroids.2024.109544

Menghuan Wu , Zhaoxiang Wang

{"title":"Relative fat mass (RFM) is linked to testosterone deficiency in adult males","authors":"Menghuan Wu , Zhaoxiang Wang","doi":"10.1016/j.steroids.2024.109544","DOIUrl":"10.1016/j.steroids.2024.109544","url":null,"abstract":"<div><h3>Purpose</h3><div>Relative fat mass (RFM) is a novel obesity assessment metric, and we aim to explore the relationship between RFM and testosterone deficiency in US adult males.</div></div><div><h3>Methods</h3><div>This study included data from adult males aged 20–59 years from the NHANES 2013–2014 and 2015–2016 cycles. RFM was calculated using a linear equation based on height and waist circumference (WC). Testosterone deficiency was defined as a total serum testosterone level of less than 300 ng/dL. Logistic regression, subgroup analysis, and smooth curve fitting were employed to explore the relationship between RFM and the risk of testosterone deficiency.</div></div><div><h3>Results</h3><div>This study included 3243 participants, with 771 diagnosed with testosterone deficiency. Testosterone-deficient individuals exhibited significantly higher RFM levels compared to those with normal testosterone levels (31.23 ± 0.23 vs. 27.16 ± 0.19, <strong><em>P</em></strong> < 0.001). After adjusting for confounding variables, a positive correlation emerged between RFM and testosterone deficiency (OR = 1.16, 95 %CI = 1.11–1.22, <strong><em>P</em></strong> < 0.001). Smooth curve fitting further supported this relationship. Subgroup analysis did not identify any special populations. Receiver operating curve (ROC) analysis results indicated that RFM showed greater effectiveness compared to body mass index (BMI) and WC.</div></div><div><h3>Conclusions</h3><div>Elevated RFM levels were found to be linked to the risk of testosterone deficiency. Further studies on RFM hold promise to evaluate and address testosterone deficiency.</div></div>","PeriodicalId":21997,"journal":{"name":"Steroids","volume":"214 ","pages":"Article 109544"},"PeriodicalIF":2.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0