Stem Cells International最新文献

{"title":"VCAM-1 Promotes Angiogenesis of Bone Marrow Mesenchymal Stem Cells Derived from Patients with Trauma-Induced Osteonecrosis of the Femoral Head by Regulating the Apelin/CCN2 Pathway.","authors":"Yiming Shao,&nbsp;Lei Sun,&nbsp;Baodong Ma,&nbsp;Ranran Jin,&nbsp;Yueyao Ban,&nbsp;Ruibo Li,&nbsp;Jianfa Wang,&nbsp;Hongkai Lian,&nbsp;Han Yue","doi":"10.1155/2023/6684617","DOIUrl":"10.1155/2023/6684617","url":null,"abstract":"<p><p>Trauma-induced osteonecrosis of the femoral head (TI-ONFH) is a pathological process in which the destruction of blood vessels supplying blood to the femoral head causes the death of bone tissue cells. Vascular cell adhesion molecule 1 (VCAM-1) has been shown to have potent proangiogenic activity, but the role in angiogenesis of TI-ONFH is unclear. In this work, we discovered that VCAM-1 was significantly downregulated in the bone marrow mesenchymal stem cells (BMSCs) derived from patients with TI-ONFH. Subsequently, we constructed BMSCs overexpressing VCAM-1 using a lentiviral vector. VCAM-1 enhances the migration and angiogenesis of BMSCs. We further performed mRNA transcriptome sequencing to explore the mechanisms by which VCAM-1 promotes angiogenesis. Gene ontology biological process enrichment analysis demonstrated that upregulated differentially expressed genes (DEGs) were related to blood vessel development. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that upregulated DEGs were engaged in the Apelin signaling pathway. Apelin-13 is the endogenous ligand of the APJ receptor and activates this G protein-coupled receptor. Treatment with Apelin-13 activated the Apelin signaling pathway and suppressed the expression of cellular communication network factor 2 in BMSCs. Furthermore, Apelin-13 also inhibits the migration and angiogenesis of VCAM-1-BMSCs. In summary, VCAM-1 plays an important role in vascular microcirculation disorders of TI-ONFH, which provides a new direction for the molecular mechanism and treatment of TI-ONFH.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2023 ","pages":"6684617"},"PeriodicalIF":4.3,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49692410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dental Pulp Stem Cells for Bone Tissue Engineering: A Literature Review.","authors":"Xiaolei Bai,&nbsp;Ruijue Cao,&nbsp;Danni Wu,&nbsp;Huicong Zhang,&nbsp;Fan Yang,&nbsp;Linhong Wang","doi":"10.1155/2023/7357179","DOIUrl":"10.1155/2023/7357179","url":null,"abstract":"<p><p>Bone tissue engineering (BTE) is a promising approach for repairing and regenerating damaged bone tissue, using stem cells and scaffold structures. Among various stem cell sources, dental pulp stem cells (DPSCs) have emerged as a potential candidate due to their multipotential capabilities, ability to undergo osteogenic differentiation, low immunogenicity, and ease of isolation. This article reviews the biological characteristics of DPSCs, their potential for BTE, and the underlying transcription factors and signaling pathways involved in osteogenic differentiation; it also highlights the application of DPSCs in inducing scaffold tissues for bone regeneration and summarizes animal and clinical studies conducted in this field. This review demonstrates the potential of DPSC-based BTE for effective bone repair and regeneration, with implications for clinical translation.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2023 ","pages":"7357179"},"PeriodicalIF":4.3,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49692409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: lncRNA HOTTIP Recruits EZH2 to Inhibit PTEN Expression and Participates in IM Resistance in Chronic Myeloid Leukemia.","authors":"Stem Cells International","doi":"10.1155/2023/9760693","DOIUrl":"10.1155/2023/9760693","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/9993393.].</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2023 ","pages":"9760693"},"PeriodicalIF":4.3,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10567471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41213426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells.","authors":"Xuerui Yao, Ji Hyun Kang, Kee-Pyo Kim, Hyogeun Shin, Zhe-Long Jin, Hao Guo, Yong-Nan Xu, Ying-Hua Li, Sai Hali, Jeongwoo Kwon, Hyeonwoo La, Chanhyeok Park, Yong-June Kim, Lin Wang, Kwonho Hong, Qilong Cao, Il-Joo Cho, Nam-Hyung Kim, Dong Wook Han","doi":"10.1155/2023/3320211","DOIUrl":"10.1155/2023/3320211","url":null,"abstract":"<p><p>Brain organoids have been considered as an advanced platform for <i>in vitro</i> disease modeling and drug screening, but numerous roadblocks exist, such as lack of large-scale production technology and lengthy protocols with multiple manipulation steps, impeding the industrial translation of brain organoid technology. Here, we describe the high-speed and large-scale production of midbrain organoids using a high-throughput screening-compatible platform within 30 days. Micro midbrain organoids (<i>µ</i>MOs) exhibit a highly uniform morphology and gene expression pattern with minimal variability. Notably, <i>µ</i>MOs show dramatically accelerated maturation, resulting in the generation of functional <i>µ</i>MOs within only 30 days of differentiation. Furthermore, individual <i>µ</i>MOs display highly consistent responsiveness to neurotoxin, suggesting their usefulness as an <i>in vitro</i> high-throughput drug toxicity screening platform. Collectively, our data indicate that <i>µ</i>MO technology could represent an advanced and robust platform for <i>in vitro</i> disease modeling and drug screening for human neuronal diseases.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2023 ","pages":"3320211"},"PeriodicalIF":3.8,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10558263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41153711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose-Derived Stem Cell Exosomes Antagonize the Inhibitory Effect of Dihydrotestosterone on Hair Follicle Growth by Activating Wnt/<i>β</i>-Catenin Pathway.","authors":"Xin Tang,&nbsp;Cuixiang Cao,&nbsp;Yunxiao Liang,&nbsp;Le Han,&nbsp;Bin Tu,&nbsp;Miao Yu,&nbsp;Miaojian Wan","doi":"10.1155/2023/5548112","DOIUrl":"10.1155/2023/5548112","url":null,"abstract":"<p><p>The most prevalent type of alopecia is androgenetic alopecia (AGA), which has a high prevalence but no effective treatment. Elevated dihydrotestosterone (DHT) level in the balding area was usually thought to be critical in the pathophysiology of AGA. The canonical Wnt/<i>β</i>-catenin signaling pathway plays a key role in promoting hair follicle development and sustaining the hair follicle cycle. Adipose-derived stem cell exosomes (ADSC-Exos) are widely used in the field of regenerative medicine due to the advantages of being cell free and immune privileged. Still, few studies have reported the therapeutic effect on hair disorders. As a result, we sought to understand how ADSC-Exos affected hair growth and explore the possibility that ADSC-Exos could counteract the hair-growth-inhibiting effects of DHT. This research using human hair follicle organs, in vitro dermal papilla cells, and in vivo animal models showed that ADSC-Exos not only encouraged healthy hair growth but also counteracted the inhibitory effects of DHT on hair growth. Additionally, we discovered that ADSC-Exos increased Ser9 phosphorylated glycogen synthase kinase-3<i>β</i> levels and facilitated nuclear translocation of <i>β</i>-catenin, which may have been blocked by the specific Wnt/<i>β</i>-catenin signaling pathway inhibitor dickkopf-related protein 1. Our findings suggested that ADSC-Exos are essential for hair regeneration, which is anticipated to open up new therapeutic possibilities for clinical alopecia, particularly for the treatment of AGA.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2023 ","pages":"5548112"},"PeriodicalIF":4.3,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10551537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41131338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Mesenchymal Stem Cells-Derived Exosome Mimetic Vesicles Regulation of the MAPK Pathway and ROS Levels Inhibits Glucocorticoid-Induced Apoptosis in Osteoblasts.","authors":"Hongxu Lu,&nbsp;Zhaoxia Zhang,&nbsp;Zhaoying Wang,&nbsp;Jinkui Wang,&nbsp;Tao Mi,&nbsp;Liming Jin,&nbsp;Xin Wu,&nbsp;Junyi Luo,&nbsp;Yimeng Liu,&nbsp;Junhong Liu,&nbsp;Wenquan Cai,&nbsp;Peng Guo,&nbsp;Dawei He","doi":"10.1155/2023/5537610","DOIUrl":"https://doi.org/10.1155/2023/5537610","url":null,"abstract":"<p><strong>Background: </strong>Long-term extensive use of glucocorticoids will lead to hormonal necrosis of the femoral head, and osteoblasts play an important role in the prevention of osteonecrosis. However, there is no complete cure for necrosis of the femoral head. Mesenchymal stem cell- (MSCs-) derived exosomes are widely used for the repair of various tissue lesions. Therefore, the aim of this study was to investigate the mechanism of dexamethasone- (DEX-) induced osteoblast apoptosis and the therapeutic effect of human umbilical cord MSC- (hucMSC-) derived exosome mimetic vesicles (EMVs) on osteoblast-induced apoptosis by DEX.</p><p><strong>Methods: </strong>The viability and apoptosis of primary MC3T3-E1 cells were determined by the Cell Counting Kit-8 (CCK-8), FITC-Annexin V/PI staining and immunoblot. The intracellular levels of reactive oxygen species (ROS) after DEX treatment were measured by 2', 7' -dichlorodihydrofluorescein diacetate (DCFH-DA) staining. In this study, hucMSC-EMVs and <i>N</i>-acetyl-l-cysteine (NAC) were used as therapeutic measures. The expression of B-cell lymphoma 2-associated X, Bcl 2, HO-1, and nuclear factor erythroid-derived 2-like 2 and MAPK- signaling pathway in osteogenic cell MC3T3-E1 cells treated with Dex was analyzed by the immunoblotting.</p><p><strong>Results: </strong>DEX significantly induced osteoblasts MC3T3-E1 apoptosis and ROS accumulation. MAPK-signaling pathway was activated in MC3T3-E1 after DEX treatment. hucMSC-EMVs intervention significantly downregulated DEX-induced MAPK-signaling pathway activation and ROS accumulation. In addition, hucMSC-EMVs can reduce the apoptosis levels in osteoblast MC3T3-E1 cells induced by DEX.</p><p><strong>Conclusions: </strong>Our study confirmed that hucMSC-EMVs regulates MAPK-signaling pathway and ROS levels to inhibit DEX-induced osteoblast apoptosis.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2023 ","pages":"5537610"},"PeriodicalIF":4.3,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10533242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41177002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promising Markers in the Context of Mesenchymal Stem/Stromal Cells Subpopulations with Unique Properties.","authors":"Agnieszka Smolinska,&nbsp;Aleksandra Bzinkowska,&nbsp;Paulina Rybkowska,&nbsp;Magdalena Chodkowska,&nbsp;Anna Sarnowska","doi":"10.1155/2023/1842958","DOIUrl":"https://doi.org/10.1155/2023/1842958","url":null,"abstract":"<p><p>The heterogeneity of the mesenchymal stem/stromal cells (MSCs) population poses a challenge to researchers and clinicians, especially those observed at the population level. What is more, the lack of precise evidences regarding MSCs developmental origin even further complicate this issue. As the available evidences indicate several possible pathways of MSCs formation, this diverse origin may be reflected in the unique subsets of cells found within the MSCs population. Such populations differ in specialization degree, proliferation, and immunomodulatory properties or exhibit other additional properties such as increased angiogenesis capacity. In this review article, we attempted to identify such outstanding populations according to the specific surface antigens or intracellular markers. Described groups were characterized depending on their specialization and potential therapeutic application. The reports presented here cover a wide variety of properties found in the recent literature, which is quite scarce for many candidates mentioned in this article. Even though the collected information would allow for better targeting of specific subpopulations in regenerative medicine to increase the effectiveness of MSC-based therapies.</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2023 ","pages":"1842958"},"PeriodicalIF":4.3,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10533301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41113981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: Severity Assessment of COVID-19 Using a CT-Based Radiomics Model.","authors":"Stem Cells International","doi":"10.1155/2023/9786985","DOIUrl":"https://doi.org/10.1155/2023/9786985","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2021/2263469.].</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2023 ","pages":"9786985"},"PeriodicalIF":4.3,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41178277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: The Mechanism of Oxidative Stress in Cells Isolation, Identification, and Genome-Wide Sequence Analysis of Nitrite Amylolytic Bacillus.","authors":"Stem Cells International","doi":"10.1155/2023/9819567","DOIUrl":"10.1155/2023/9819567","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/9521900.].</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2023 ","pages":"9819567"},"PeriodicalIF":3.8,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513821/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41179960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: Segmentation Algorithm of Magnetic Resonance Imaging Glioma under Fully Convolutional Densely Connected Convolutional Networks.","authors":"Stem Cells International","doi":"10.1155/2023/9842197","DOIUrl":"https://doi.org/10.1155/2023/9842197","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/8619690.].</p>","PeriodicalId":21962,"journal":{"name":"Stem Cells International","volume":"2023 ","pages":"9842197"},"PeriodicalIF":4.3,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41149892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}