Solid state nuclear magnetic resonance最新文献

{"title":"Extending 17O transverse relaxation measurement to satellite transitions as a direct probe of molecular dynamics in solids","authors":"Yizhe Dai ,&nbsp;Ivan Hung ,&nbsp;Zhehong Gan ,&nbsp;Gang Wu","doi":"10.1016/j.ssnmr.2025.102004","DOIUrl":"10.1016/j.ssnmr.2025.102004","url":null,"abstract":"<div><div>We report utilization of transverse relaxation rate (<em>R</em>₂) of ¹⁷O (<em>I</em> = 5/2) satellite transitions (STs) as a probe of molecular dynamics in solids. A simple theoretical model using spectral density functions is proposed to describe the general <em>R</em>₂ behaviors of half-integer quadrupolar nuclei in solids in the presence of molecular motion (or chemical exchange). Experimental ¹⁷O <em>R</em>₂ data recorded for both CT and ST from ¹⁷O-labeled NaNO₂ over a large temperature range are used to verify the theoretical predictions. Our theoretical model is shown to be fully consistent with a full quantum mechanical treatment of the chemical exchange problem involving half-integer quadrupolar nuclei in solids by numerically solving the Liouville-von Neumann equation. The new ¹⁷O ST <em>R</em>₂ method was also applied to study the carboxylate flipping motion in two [¹⁷O]carboxylic acid-pyridine adducts in the solid state. The advantages of the ST <em>R</em>₂ approach are discussed. This ST <em>R</em>₂ approach adds a new dimension to the currently available CT-based solid-state NMR techniques for probing molecular motion in solids.</div></div>","PeriodicalId":21937,"journal":{"name":"Solid state nuclear magnetic resonance","volume":"137 ","pages":"Article 102004"},"PeriodicalIF":1.8,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Solid-state NMR and DNP of heterogeneous biomaterials and cellular systems","authors":"Patrick C.A. van der Wel ,&nbsp;Tuo Wang","doi":"10.1016/j.ssnmr.2025.102003","DOIUrl":"10.1016/j.ssnmr.2025.102003","url":null,"abstract":"","PeriodicalId":21937,"journal":{"name":"Solid state nuclear magnetic resonance","volume":"137 ","pages":"Article 102003"},"PeriodicalIF":1.8,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solid-state NMR observation of chitin in whole cells by indirect 15N detection with NC, NCC, CNC and CNCC polarization transfers","authors":"Loic Delcourte ,&nbsp;Mélanie Berbon ,&nbsp;Marion Rodriguez ,&nbsp;Laurence Delhaes ,&nbsp;Birgit Habenstein ,&nbsp;Antoine Loquet","doi":"10.1016/j.ssnmr.2025.102002","DOIUrl":"10.1016/j.ssnmr.2025.102002","url":null,"abstract":"<div><div>Chitin is the most important nitrogen-containing polysaccharide found on Earth. This polysaccharide is a polymer of an N-acetylglucosamine and it is a crucial structural component of fungal cell walls and crustaceans. Magic-angle spinning solid-state NMR is emerging as a powerful analytical approach to study polysaccharides in the context of intact cell walls and whole cells. The presence of an acetamido group in chitin is attractive for ¹⁵N solid-state NMR. Here we investigate the use of various multi-step polarization transfer experiments incorporating indirect ¹⁵N detection at moderate spinning frequency, adapted from pulse sequences commonly employed for residue resonance assignment in biosolid proteins. The ¹³C,¹⁵N chitin spin topology slightly differs from amino acids, and we discussed the use of frequency-selective ¹⁵N-¹³C cross-polarization transfers followed by broadband or frequency-selective homonuclear ¹³C–¹³C transfers to detect chitin resonances. Demonstrated here for chitin found in the cell wall of the fungus <em>Aspergillus fumigatus</em>, the use of indirect ¹⁵N detection through multi-step polarization transfers could be advantageous to investigate more complex nitrogen-containing polysaccharides found in whole cells and peptidoglycan samples.</div></div>","PeriodicalId":21937,"journal":{"name":"Solid state nuclear magnetic resonance","volume":"137 ","pages":"Article 102002"},"PeriodicalIF":1.8,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly sensitive measurements of methylene dynamics with a frequency-selective double-quantum sideband method","authors":"Jacob Mayer ,&nbsp;Frédéric A. Perras","doi":"10.1016/j.ssnmr.2025.101994","DOIUrl":"10.1016/j.ssnmr.2025.101994","url":null,"abstract":"<div><div>Probing the fast dynamics of surface sites using NMR spectroscopy is highly challenging owing to the sites' high dilution and the difficulties often associated with isotopic enrichment. Intra-CH₂ ¹H–¹H dipolar couplings are ideal probes of motions given that they only involve ¹H's and the tensor has a well-defined size and orientation. We introduce a frequency-selective variant of the double-quantum sideband method to measure like-spin ¹H–¹H dipolar coupling constants. The experiment dramatically reduces the instrument time required to measure dynamically-averaged intra-CH₂ dipolar couplings. We demonstrate the performance of the sequence using silica-supported silanes as model highly-mobile surface species.</div></div>","PeriodicalId":21937,"journal":{"name":"Solid state nuclear magnetic resonance","volume":"136 ","pages":"Article 101994"},"PeriodicalIF":1.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deuteron-proton isotope correlation spectroscopy at high magnetic fields","authors":"Neethu Thomas ,&nbsp;Claire Welton ,&nbsp;Tomasz Pawlak ,&nbsp;Parth Raval ,&nbsp;Julien Trébosc ,&nbsp;Sheetal K. Jain ,&nbsp;G.N. Manjunatha Reddy","doi":"10.1016/j.ssnmr.2025.101993","DOIUrl":"10.1016/j.ssnmr.2025.101993","url":null,"abstract":"<div><div>A cross-polarization ²H–¹H isotope correlation spectroscopy (CP-iCOSY) approach is presented for characterizing a deuterated amino acid, pharmaceutical compound and a solid formulation. This can be achieved by isotopic enrichment in conjunction with high magnetic field (28.2 T) and fast magic-angle spinning (MAS), enabling the rapid detection of ²H NMR spectra in a few seconds to minutes. Specifically, two-dimensional (2D) ²H–¹H CP-iCOSY experiment allows the local structures and through-space interactions in a partially deuterated compounds to be elucidated. In doing so, we compare conventional spin-lock and rotor-echo-short-pulse-irradiation ^RESPIRATIONCP sequences for acquiring 2D ¹H–²H correlation spectra. The ^RESPIRATIONCP sequence allows the detection of 2D peaks at lower CP contact times (0.1–1 ms) than the conventional CP (0.2–4 ms) sequence. Analysis of partially deuterated L-histidine·HCl·H₂O and dopamine.HCl is presented, in which the detection of 2D peaks corresponding to ²H–¹H pairs separated by greater than 4 Å distance demonstrates the potential of the presented approach for the characterization of packing interactions. These results are corroborated by NMR crystallography analysis using the Gauge-Including Projector Augmented-Wave (GIPAW) approach.</div></div>","PeriodicalId":21937,"journal":{"name":"Solid state nuclear magnetic resonance","volume":"136 ","pages":"Article 101993"},"PeriodicalIF":1.8,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143418551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal control: From sensitivity improvement to alternative pulse-sequence design in solid-state NMR","authors":"Jan Blahut ,&nbsp;Zdeněk Tošner","doi":"10.1016/j.ssnmr.2024.101984","DOIUrl":"10.1016/j.ssnmr.2024.101984","url":null,"abstract":"<div><div>Exciting developments in new experimental methods for multidimensional solid-state NMR spectroscopy have recently been achieved using optimal-control theory. These results, in turn, have triggered the development of new pulse sequences based on traditional analytical theories. This trend article summarises the key steps leading to these advancements. It also describes additional applications of optimal control beyond structural biology and envisions similar progress in the NMR of solid materials. Despite attractive features of optimal-control pulse sequences demonstrated in the proof-of-concept studies, their experimental utilization remains sparse, probably due to the lack of awareness among experimentalists. We hope this mini-review helps to spread optimal-control methods into routine experimental workflows. Furthermore, we offer a personal outlook on how numerical optimisations could in general enhance the experimental capabilities of solid-state NMR in the near future, with optimal control serving as a pioneer exploring new possibilities.</div></div>","PeriodicalId":21937,"journal":{"name":"Solid state nuclear magnetic resonance","volume":"135 ","pages":"Article 101984"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spin-dynamics and efficiency of single 14N-1H cross-polarization at fast magic angle spinning in solids","authors":"Vipin Agarwal ,&nbsp;Sreejith Raran-Kurussi ,&nbsp;Yusuke Nishiyama","doi":"10.1016/j.ssnmr.2025.101992","DOIUrl":"10.1016/j.ssnmr.2025.101992","url":null,"abstract":"<div><div>The naturally abundant ¹⁴N isotope (&gt;99 %) is sparingly employed for characterization in solid-state nuclear magnetic resonance (NMR) despite the importance of nitrogen atoms in shaping molecular structures and properties. This inhibition can be attributed to large quadrupolar couplings (∼several MHz), resulting in more involved spin methodologies for ¹⁴N nuclei. Experimentally, spin-½ nuclei are utilized for excitation and detection through two-way (¹H→¹⁴N→¹H) polarization transfer between spin-½ nuclei and ¹⁴N. Herein, we show direct ¹⁴N spin excitation followed by ¹⁴N→¹H cross-polarization (CP) is an efficient method for polarization transfer even for ¹⁴N spins with a large quadrupolar coupling constant (3–4 MHz). This contrasts previous studies, which indicate that ¹H-¹⁴N spectra can only be observed with a pair of at least a rotor period-long symmetric ¹⁴N pulses (<em>J. Chem. Phys. 151</em> (2019) <em>154202</em>). The ¹⁴N→¹H CP spin dynamics have been experimentally established and can be explained in analogy to spin-½ Hartmann-Hahn CP if visualized in the quadrupolar jolting frame. The ¹⁴N→¹H CP is ∼1.9–2.7 times more efficient in polarization transfer than other ¹⁴N edited experiments. Considering shorter ¹⁴N T₁ relaxation times compared to protons, ¹⁴N edited spectra were recorded using ¹⁴N→¹H CP, resulting in enhanced sensitivity per unit of time.</div></div>","PeriodicalId":21937,"journal":{"name":"Solid state nuclear magnetic resonance","volume":"136 ","pages":"Article 101992"},"PeriodicalIF":1.8,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applications of NMR based methodologies investigating the behavior of lignin and cellulose towards bio-based carbon fibers production","authors":"Feryal Guerroudj ,&nbsp;Lukas Fliri ,&nbsp;Jenny Bengtsson ,&nbsp;Leandro Cid Gomes ,&nbsp;Tristan Gazzola ,&nbsp;Michael Hummel ,&nbsp;Diana Bernin","doi":"10.1016/j.ssnmr.2024.101977","DOIUrl":"10.1016/j.ssnmr.2024.101977","url":null,"abstract":"<div><div>Cellulose–lignin blends are proposed as alternative precursors for carbon fiber (CF) production, offering a potential sustainable and cost-effective alternative to the expensive fossil-based polymers currently used. The characteristics of the precursor fibers including their crystallinity, the incorporated chemical structures and the distribution of the biopolymers have a significant influence on their carbonization behavior and the properties of the CFs. They are partly determined by the composition of the bio-based resources and the conditions used during the fiber fixation, i.e. the coagulation, an important processing step. In this work, ¹³C solid and 2D solution NMR methodologies were applied to investigate the impact of coagulation and thermostabilization conditions on cellulose and cellulose-lignin blends using a thin film model. Solid state NMR spectroscopy showed that the choice of the anti-solvent influenced the proportion of cellulose II <em>versus</em> amorphous regions in the coagulated films. Independent of the presence of lignin, the choice of anti-solvent seems to impact the rate of thermal reactions. After thermostabilization at 245 °C, the samples were investigated using a solution NMR protocol devised for cellulosic materials. At 275 °C, most of the samples became insoluble for solution NMR. However, solid state NMR revealed further changes in the chemical composition, which were dependent on both the presence of lignin and the choice of anti-solvent. This multi-faceted approach combining solid state and 2D solution NMR techniques provides a comprehensive understanding of the cellulose structure and the products formed for cellulose-lignin-based CFs, which is crucial for optimizing their properties and potential applications.</div></div>","PeriodicalId":21937,"journal":{"name":"Solid state nuclear magnetic resonance","volume":"134 ","pages":"Article 101977"},"PeriodicalIF":1.8,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"17O NMR relaxation measurements for investigation of molecular dynamics in static solids using sodium nitrate as a model compound","authors":"Liliya Vugmeyster ,&nbsp;Riqiang Fu ,&nbsp;Dmitry Ostrovsky","doi":"10.1016/j.ssnmr.2024.101976","DOIUrl":"10.1016/j.ssnmr.2024.101976","url":null,"abstract":"<div><div>¹⁷O NMR methods are emerging as a powerful tool for determination of structure and dynamics in materials and biological solids. We present experimental and theoretical frameworks for measurements of ¹⁷O NMR relaxation times in static solids focusing on the excitation of the central transition of the ¹⁷O spin 5/2 system. We employ ¹⁷O-enriched NaNO₃ as a model compound, in which the nitrate oxygen atoms undergo 3-fold jumps. Rotating frame (<span><math><mrow><msub><mi>T</mi><mrow><mn>1</mn><mi>ρ</mi></mrow></msub></mrow></math></span>), transverse (<span><math><mrow><msub><mi>T</mi><mn>2</mn></msub></mrow></math></span>) and longitudinal (<span><math><mrow><msub><mi>T</mi><mn>1</mn></msub></mrow></math></span>) relaxation times as well as line shapes were measured for the central transition in the 280 to 195 K temperature range at 14.1 and 18.8 T field strengths. We conduct experimental and theoretical comparison between different relaxation methods and demonstrate the advantage of combining data from multiple relaxation time and line shape measurements to obtain a more accurate determination of the dynamics as compared to either of the techniques alone. The computational framework for relaxation of spin 5/2 nuclei is developed using the numerical integration of the Liouville − von Neumann equation.</div></div>","PeriodicalId":21937,"journal":{"name":"Solid state nuclear magnetic resonance","volume":"134 ","pages":"Article 101976"},"PeriodicalIF":1.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142691155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solid-state NMR compositional analysis of sputum from people with cystic fibrosis","authors":"Kathy Duong ,&nbsp;Evan Moss ,&nbsp;Courtney Reichhardt","doi":"10.1016/j.ssnmr.2024.101975","DOIUrl":"10.1016/j.ssnmr.2024.101975","url":null,"abstract":"<div><div>People with the genetic disease cystic fibrosis (CF) often have chronic airway infections and produce airway secretions called sputum. A better understanding of sputum composition is desired in order to track changes in response to CF therapeutics and to improve laboratory models for the study of CF airway infections. The glycosylated protein mucin is a primary component. Along with extracellular DNA, mucin gives rise to the high viscoelasticity of sputum, which inhibits airway clearance and is thought to promote chronic airway infections in people with CF. Past studies of sputum composition identified additional biomolecular components of sputum including other proteins, both glycosylated and not glycosylated, free amino acids, and lipids. Typically, studies of sputum, as well as other complex biological materials, have focused on soluble or isolated components. Solid-state NMR is not limited to the study of soluble components. Instead, it can provide molecular-level information about insoluble biological samples. Additionally, solid-state NMR can provide information about sample composition without requiring any processing of the sample, eliminating the possibility of misestimating certain components due to insolubility or potential sample loss in isolation steps. In this study, we used both ¹³C and ³¹P CPMAS to investigate the total composition of sputum samples obtained from six people with CF. We compared these spectra to those of commercially available mucin, DNA, and phospholipid samples. Lastly, we performed complementary biochemical analyses to identify specific proteins present in the sputum samples. Overall, our findings provide insight into the composition of unprocessed sputum samples from people with CF, which can be used as a benchmark for future investigations of CF and infections in the airways of people with CF. Further, this study provides opportunities to expand the solid-state NMR approach to include dynamic nuclear polarization (DNP) to obtain high-resolution information of sputum and similar biological samples that are not feasible to isotopically enrich.</div></div>","PeriodicalId":21937,"journal":{"name":"Solid state nuclear magnetic resonance","volume":"134 ","pages":"Article 101975"},"PeriodicalIF":1.8,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}