Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology最新文献_第9页

Enzymatic Production of Fish Protein Hydrolysates in A Pilot Plant Scale 在中试工厂规模上酶促生产鱼蛋白水解物

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2019-08-30 DOI: 10.15578/SQUALEN.V14I2.398

P. Martosuyono, Y. N. Fawzya, G. Patantis, S. Sugiyono

{"title":"Enzymatic Production of Fish Protein Hydrolysates in A Pilot Plant Scale","authors":"P. Martosuyono, Y. N. Fawzya, G. Patantis, S. Sugiyono","doi":"10.15578/SQUALEN.V14I2.398","DOIUrl":"https://doi.org/10.15578/SQUALEN.V14I2.398","url":null,"abstract":"Protease enzyme produced from Bacillus sp was employed to hydrolyze fish protein hydrolysates (FPH) under controlled conditions at a batch-pilot plant scale-process. Thirty kilograms of fish meat was mincedand mixed with 60 liters of water in 100 liters stainless steel vessel and 20,000 units of protease enzyme was added per kg of fish. Hydrolysis of fish was carried out at 55 oC for 6 hours. Multi stage of filtration were done to separate the FPH from unhydrolized fish residue. Mass balance were carried out to determine the rate of hydrolysis and yields. W ithout pH adjustment, 80% of substrate hydrolyzed could be achieved in 6 hour at 55 °C. Three kinds of products were recovered from the process, i.e solid residue, liquid FPH as filtration product, and spray dried FPH. Hydrolysis of 30 kg of fish meat substrate producing 1.7-2.0 kg of unhydrolyzed residue and 70 L of liquid FPH. Afterspray drying process of liquid FPH, 13 kg of FPH powder was recovered. The proximate and amino acid analysis of spray dried FPH showed that the FPH containing 20% of protein, rich in amino acids especially lysine and leucineand the residue still had 85,36% of protein (dry basis) that could be utilized for other purpose.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76271790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Preliminary Study on Kecalok, An Indigenous Shrimp Sauce from Indonesia 印尼本土虾酱Kecalok的初步研究

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2019-08-30 DOI: 10.15578/SQUALEN.V14I2.386

Mahrus Ali, Ardiansyah Kurniawan, N. M. Noor

引用次数: 3

Back Cover Squalen Bulletin Vol. 14 No. 2 Tahun 2019 封底Squalen Bulletin第14卷第2期Tahun 2019

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2019-08-30 DOI: 10.15578/squalen.v14i2.402

Squalen Bulletin

引用次数: 0

Preface Squalen Bulletin Vol. 14 No. 1 Tahun 2019 《角鲨通报》第14卷第1期，2019年

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2019-05-31 DOI: 10.15578/squalen.v14i1.395

Squalen Bulletin

引用次数: 0

Color Stability of Phycoerythrin Crude Extract (PECE) from Rhodomonas Salina Toward Physicochemical Factors 盐渍Rhodomonas Salina藻红蛋白粗提物(PECE)的理化稳定性

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2019-05-31 DOI: 10.15578/SQUALEN.V14I1.379

E. Marraskuranto, T. Raharjo, R. Kasiamdari, T. R. Nuringtyas

{"title":"Color Stability of Phycoerythrin Crude Extract (PECE) from Rhodomonas Salina Toward Physicochemical Factors","authors":"E. Marraskuranto, T. Raharjo, R. Kasiamdari, T. R. Nuringtyas","doi":"10.15578/SQUALEN.V14I1.379","DOIUrl":"https://doi.org/10.15578/SQUALEN.V14I1.379","url":null,"abstract":"Rhodomonas salina produces Cr-phycoerythrin545 as its designated phycoerythrin (PE) with an absorption maximum at 545 nm and a shoulder 564 nm. PE has potential to be applied as colorants, pharmaceutical agents, and fluorescent dye tags. The stability of the PE color is influenced by the physicochemical factors of the solution. This study aimed to analyze the color stability of PECE against chemical (ethanol and pH) and physical (light and temperature) factors. PECE was prepared from freeze-dried biomass of R. salina and was extracted in phosphate buffer solution (pH = 6.0) using a freeze-thaw method in -25 oC (2 hours) and 4 oC (24 hours). The resulting extract was concentrated and dried in a freeze-dryer. Analyses were conducted using UV-visible and fluorescence spectrophotometer. PECE showed color stability against light of white fluorescent lamp exposure up to 8 hours, temperature exposure up to 40 oC, ethanol solution up to concentration of 20 % (v/v), and pH range 3.9-8.42. Results from this study can be useful for extraction, purification, and future application of Cr-PE545.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88033541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Screening of Marine-Derived Fungi Extracts as Antioxidant, Tyrosinase Inhibitor, and Antiglycation 海洋真菌提取物抗氧化、酪氨酸酶抑制剂和抗糖化作用的筛选

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2019-05-31 DOI: 10.15578/SQUALEN.V14I1.366

M. Nursid, E. Marraskuranto, Dilaika Septorini, I. Batubara

{"title":"Screening of Marine-Derived Fungi Extracts as Antioxidant, Tyrosinase Inhibitor, and Antiglycation","authors":"M. Nursid, E. Marraskuranto, Dilaika Septorini, I. Batubara","doi":"10.15578/SQUALEN.V14I1.366","DOIUrl":"https://doi.org/10.15578/SQUALEN.V14I1.366","url":null,"abstract":"Marine-derived fungi are of great interest as new promising sources of bioactive secondary metabolites. The aim of this study was to determine the antioxidant activity, tyrosinase inhibitor and antiglycation of marine-derrived fungi collected from Kepulauan Seribu Marine National Park, Indonesia. Antioxidant screening was determined using the 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. The tyrosinase inhibitor was screened using L-tyrosine substrate, while the antiglycation test was determined by the ability to inhibit the formation of advanced glycation end products (AGEs). A total of 28 marine fungi isolates had been screened for their activities. Mycelium extract of MFP 271 had the best antioxidant activity with the IC50 of 287.25 ± 50 µg/mL. Mycelium extract of MFP 277 had the best tyrosinase inhibitory activity with the IC50 of 586.42 µg/mL. The MFP 274-broth extract had the highest antiglycation activity with the IC50 value of 298.57 µg/mL. Based on the screening, the crude extracts were considered weak as antioxidant, but tyrosinase inhibitor and antiglycation activity of MFP 277 and MFP 274 are needed to investigate in depth activities.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73760166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Back Cover Squalen Bulletin Vol. 14 No. 1 Tahun 2019 封底《角鲨通报》第十四卷第1期2019

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2019-05-31 DOI: 10.15578/squalen.v14i1.390

Squalen Bulletin

引用次数: 0

Front Cover Squalen Bulletin Vol. 14 No. 1 Tahun 2019 《角鲨通报》第14卷第1期封面2019

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2019-05-31 DOI: 10.15578/squalen.v14i1.389

Squalen Bulletin

引用次数: 0

A beginner’s guide to molecular identification of seaweed 海藻分子鉴定的初学者指南

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2019-05-31 DOI: 10.15578/SQUALEN.V14I1.384

G. Zuccarello, N. Paul

{"title":"A beginner’s guide to molecular identification of seaweed","authors":"G. Zuccarello, N. Paul","doi":"10.15578/SQUALEN.V14I1.384","DOIUrl":"https://doi.org/10.15578/SQUALEN.V14I1.384","url":null,"abstract":"© 2019, Squalen BMFPB. The most rapidly expanding areas for seaweed production in the world are the tropics, including Indonesia, yet these areas are also where molecular identification of local marine flora has only been sporadically employed. Furthermore, a goal for the Government of Indonesia is to diversify the types of seaweed that are being utilized, targeting valuable products and, hand in hand, to develop aquaculture techniques for these species. Morphological methods for species identification in algae are complex or unreliable, due to simple morphologies and plasticity. Therefore, it is crucial that the correct identification is made for species and varieties of commercial interest so that growth and biochemical results can be compared and contrasted between locations, across environments and over time without taxonomic ambiguity. This guide presents entry level methodologies for sample collection, DNA preservation, DNA extraction, PCR, and analyses of DNA sequence data, as a first step in the genetic characterization of both well-known cultivated species and identification of different species with potential economic properties.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87451335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

An Estimation of Endogenous Formaldehyde Exposure due to Consumption of Indonesian Opah Fish (Lampris guttatus) in Three Major Export Destination Countries 三个主要出口目的地国家食用印尼鱼(Lampris guttatus)的内源性甲醛暴露评估

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2019-05-31 DOI: 10.15578/SQUALEN.V14I1.369

Umi Anissah, Ajeng Kurniasari Putri, G. Barokah

{"title":"An Estimation of Endogenous Formaldehyde Exposure due to Consumption of Indonesian Opah Fish (Lampris guttatus) in Three Major Export Destination Countries","authors":"Umi Anissah, Ajeng Kurniasari Putri, G. Barokah","doi":"10.15578/SQUALEN.V14I1.369","DOIUrl":"https://doi.org/10.15578/SQUALEN.V14I1.369","url":null,"abstract":"The demand for Indonesian opah fish as an export product is increasing in the international market. Three countries (Malaysia, Mauritius, and Taiwan) recorded as the leading export destination of Indonesian opah fish. However, as the fish kept in a frozen state during export transportation, the endogenous formaldehyde may increase over time. This research presented the health risk assessment of population in the leading export destination countries that consumed opah fish from Indonesia. The study aimed to reveal the most potential export destination country that may accept an increasing volume of opah fish supply from Indonesia. The potency was determined from current export volume, the amount of endogenous formaldehyde content, and fish consumption at each country. The data were calculated with @Risk®7.0 software. The results showed opah fish consumed by Malaysian can be categorized as safe. Increasing the number of opah fish imported by Malaysian as much as six times, 12 times, 18 times, 27 and 36 times relatively does not cause health risks related to the presence of its endogenous formaldehyde. Moreover, opah fish consumed by Taiwanese is also safe, but with increasing the number of consumptions by more than 26 times is suspected to be potentially causing a health problem. However, opah fish consumed in Mauritius was categorized as unsafe and potentially caused health risks. Based on these results, Indonesia may consider to increase the opah fish export to Malaysia and Taiwan in the future.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86892016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4