海藻分子鉴定的初学者指南

Q3 Environmental Science
G. Zuccarello, N. Paul
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引用次数: 7

摘要

©2019,Squalen BMFPB。世界上海藻生产增长最快的地区是热带地区,包括印度尼西亚,但这些地区也是当地海洋植物群的分子鉴定只是零星使用的地区。此外,印度尼西亚政府的一个目标是使正在利用的海藻种类多样化,以有价值的产品为目标,同时为这些品种开发水产养殖技术。由于形态简单且具有可塑性,藻类物种鉴定的形态学方法复杂或不可靠。因此,对具有商业价值的物种和品种进行正确的鉴定是至关重要的,这样就可以在不同的地点、不同的环境和不同的时间对生长和生化结果进行比较和对比,而不会产生分类上的歧义。本指南介绍了样品收集、DNA保存、DNA提取、PCR和DNA序列数据分析的入门级方法,作为对已知栽培物种和具有潜在经济特性的不同物种进行遗传表征的第一步。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A beginner’s guide to molecular identification of seaweed
© 2019, Squalen BMFPB. The most rapidly expanding areas for seaweed production in the world are the tropics, including Indonesia, yet these areas are also where molecular identification of local marine flora has only been sporadically employed. Furthermore, a goal for the Government of Indonesia is to diversify the types of seaweed that are being utilized, targeting valuable products and, hand in hand, to develop aquaculture techniques for these species. Morphological methods for species identification in algae are complex or unreliable, due to simple morphologies and plasticity. Therefore, it is crucial that the correct identification is made for species and varieties of commercial interest so that growth and biochemical results can be compared and contrasted between locations, across environments and over time without taxonomic ambiguity. This guide presents entry level methodologies for sample collection, DNA preservation, DNA extraction, PCR, and analyses of DNA sequence data, as a first step in the genetic characterization of both well-known cultivated species and identification of different species with potential economic properties.
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
16 weeks
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