Journal of Biotechnology & Bioinformatics Research最新文献

{"title":"Different Approaches for Developing of Salt Tolerant Sugarcane (Saccharum officinarum)","authors":"M. Ishfaq, Fatima Zahra","doi":"10.47363/jbbr/2021(3)130","DOIUrl":"https://doi.org/10.47363/jbbr/2021(3)130","url":null,"abstract":"Sugarcane is a very important crop mostly cultivated in many regions of the world. Sugarcane is an important crop in tropical areas of the world, often being uncovered to environments with high salinity, but little is recognized of genetic variant in salt tolerance. The purpose of this work was once to examine the performance of two genetically various cultivars of sugarcane underneath specific concentrations of salinity (0, 40, eighty and a hundred and sixty mM NaCl) over a period of 30 days. SP 81–3250 was extra salt-tolerant and maintained its charge of biomass production, photosynthesis and leaf place up to one hundred sixty mM NaCl, whereas IAC 87–3396 was a sensitive to 80 mM NaCl. SP 81–3250 maintained very low concentrations of Na+ in both leaves and roots with increasing time and salinity, whereas in IAC 87–3396 the Na+ concentrations had been 2–5 instances higher. This suggests that the tolerance of SP 81–3250 to excessive salinity was due to its ability to leave out Na+ whilst taking up water from the soil, and that measurements of Na+ awareness in leaves should be used to pick out salt-tolerant genotypes for saline areas.","PeriodicalId":219036,"journal":{"name":"Journal of Biotechnology & Bioinformatics Research","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131472860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome Editing: New, Emerging, and Interesting Developments for Clinical Applications","authors":"K. Aziz","doi":"10.47363/jbbr/2021(3)127","DOIUrl":"https://doi.org/10.47363/jbbr/2021(3)127","url":null,"abstract":"Principles of Genome Editing can be applied in the various areas of medical diagnosis and treatments ---from early process design/development through maintenance of the validated state during commercial manufacturing and post-marketing surveillance. Gene editing and clinical applications comprises of systematically assessing, monitoring, and reviewing manufacturing processes and subsequently monitoring measures to control output risks. Quality risk management (QRM) principles have been described in various FDA’s regulatory guidances for several aspects of good manufacturing practices (GMPs) such as several stages of process validation and verification in the drug product lifecycle including critical quality attributes (CQA’s) and monitoring critical process parameters (CPPs). A CPP is defined as a process parameter whose variability has an impact on a CQA and, therefore, should be monitored or controlled to ensure that manufacturing process produces end product of the desired quality. FDA’s mission is to facilitate the premarket review and evaluation of new genomic products for clinical use. The FDA guidances emphasize quality management approach to design of studies by providing oversight and objective review based on risk-benefit analysis and guides the use of new medical products by providing patients organized data and appropriate labeling information in support of the new product’s intended clinical use [1-3].","PeriodicalId":219036,"journal":{"name":"Journal of Biotechnology & Bioinformatics Research","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127267230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Accuracy of Immunological Markers for Detection of Allergens by Poly Check Techniques among Patients with Bronchial Asthma in Basrah, Southren of Iraq","authors":"I. Alsaimary, Falih Hmood Mezban","doi":"10.47363/jbbr/2021(3)125","DOIUrl":"https://doi.org/10.47363/jbbr/2021(3)125","url":null,"abstract":"This study aimed to estimate specific IgE by poly check technique against various allergens. In the present study 56 environmental allergens (food, fungal, agricultural, and aeroallergens) were tested against specific IgE. This study found a very high significant allergen – specific IgE reactions with a high degrees mediated a various forms of hypersensitivity is sever in 4 and 5 stage of age groups to males asthma patients, in allergens except Cat Ep. While in females recorded severe hypersensitivity in 2, 3 and 4 stage of age groups, in allergens except cat. Ep., Plantain, hazel pollen. And Alder pollen.","PeriodicalId":219036,"journal":{"name":"Journal of Biotechnology & Bioinformatics Research","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132704864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Nutrigenomics to Microbiomics: New Interpretations","authors":"B. Riccardi, S. Resta, Roberto Fabbroni","doi":"10.47363/jbbr/2021(3)128","DOIUrl":"https://doi.org/10.47363/jbbr/2021(3)128","url":null,"abstract":"In this work we analyze the current interpretations based on the studies of Nutrigenomic and Microbiomics, according to which, at the base of many pathological and dysmetabolic manifestations, there would be or variations of single nucleotide polymorphisms (Snps), in the Nutrigenomic vision, or changes of the normal gut microbiota, in the Microbiomic conception. In particular, we analyze critically, the singular pathogenic coincidence, which for the same pathologies calls into question or Snps mutations or the presence of specific modifications of the Microbiota. Other possible causes of observed diseases are thus neglected. On the basis of the literature examined, we present a different interpretation of pathogenic causes.","PeriodicalId":219036,"journal":{"name":"Journal of Biotechnology & Bioinformatics Research","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122755451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"More about the Informational (Quantum) Healing In the Ongoing Pandemic Situation","authors":"M. Kuman","doi":"10.47363/JBBR/2020(2)124","DOIUrl":"https://doi.org/10.47363/JBBR/2020(2)124","url":null,"abstract":"In a Pandemic like this one, it is important to know that the viruses cause color changes in the aura long before the disease symptom would appear in the body. As soon as the aura changes its color, we need to start trials to restore the health. All processes in the body are ruled and regulated by a Quantum Computer in the Subconscious, which operates with the waves of the weak informational NEMF seen as aura and called Spirit. If so, we need to try to access the weak informational NEMF informationally (with prayer or meditation with affirmation) and ask for correction of the distortions in the aura (Spirit), which if not corrected would lead to a disease of the physical body at later time. EEG measurements of Russian scientists showed that during meditation and prayer the Conscious is not active. Since hypnotists put the conscious to sleep to access the Subconscious, obviously prayer and meditation with affirmation act as self-hypnosis and are ways to access the Subconscious and the Quantum Computer in it. Since the Quantum Computer operates with the waves of our weak informational NEMF (Spirit), by accessing it informationally (with prayer or meditation with affirmation), we can do informational healing. The healing with informational energy called info-energy medicine is the medicine of the future because: it is non-invasive, works fast, it is efficient, and does not have side effects.","PeriodicalId":219036,"journal":{"name":"Journal of Biotechnology & Bioinformatics Research","volume":"448 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125786280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary Assessment of DNA Profile Patterns of Neuronal Differentiation Factor (NeuroD1) Gene in the Hippocampus of Wistar Rats (Rattus norvegicus) Ingested Hyoscyamine Fraction of Datura stramonium Seeds","authors":"I. Tela, S. Musa, I. Iliya","doi":"10.47363/JBBR/2020(2)122","DOIUrl":"https://doi.org/10.47363/JBBR/2020(2)122","url":null,"abstract":"The study was designed to find out how oral ingestion of hyoscyamine fraction of D. stramonium seeds affect the DNA profiles patterns and expression of NeuroD1 gene in the hippocampus of adult Wistar rats. Fresh D. stramonium seeds were procured, identified, macerated and fractionated. Thirty-two (32) male adult Wistar rats weighed 200-250 grams were procured and divided into four groups comprised of control and three treatment groups for the study. The control received an equivalent bodyweight of normal saline, while the treatment groups received 200, 400 and 800 mg/kgbwt of hyoscyamine fraction for three weeks respectively. The animals were sacrificed, tissues harvested for histology, gel electrophoresis and gene expression studies. The fold changes were calculated and DNA profiles were determined using LabImage bio-imaging software. One-way ANOVA followed by Fisher’s multiple comparisons post-hoc test was used to obtain the difference in the expression of the gene across the groups using Minitab 17 (LLC., U.K.) statistical package software. P < 0.05 was considered statistically significant. There was a statistically significant downregulation (p = 0.001) in the NeuroD1 expression, increased DNA fragment size, decreased DNA migration distance and band volume, and necrosis of the dentate gyrus (DG) region in the hippocampus of the treated animals. In conclusion, oral ingestion of hyoscyamine fraction of D. stramonium seeds is toxic to the DNA profile patterns and expression of the NeuroD1 gene in Wistar rats.","PeriodicalId":219036,"journal":{"name":"Journal of Biotechnology & Bioinformatics Research","volume":"63 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131639177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomolecular Expression of TLR1 and TLR2 Genes Related With Prostatitis","authors":"I. Alsaimary, Hussein Naeem Aldhaheri, M. Almusafer","doi":"10.47363/JBBR/2020(2)120","DOIUrl":"https://doi.org/10.47363/JBBR/2020(2)120","url":null,"abstract":"Determine Immunogenetic expression of Toll-like receptor gene clusters related to prostatitis was carried in this investigation to give acknowledge about Role of TLR in prostatitis immunity in men. A case–control study included 135 confirmed prostatitis patients And 50 persons as a control group. Data about age, marital status, working, infertility, family history and personal information like (Infection, Allergy, Steroid therapy, Residency, Smoking, Alcohol Drinking, Blood group, Body max index (BMI) and the clinical finding for all patients of Prostatitis were collected , The molecular expression study include extracting DNA from blood of Prostatitis patients, Prostitis patients and Control group by using specific primers for conventional PCR and Real Time PCR , the amplification of all extracted DNA from blood samples was preform and confirm by using electrophoresis with (100volt/30min). The estimation revealed that the amplified DNA (PCR product) was 135bp for TLR1 (at 50 voltage for 1hour) with the presence of TLR1 was 75% of total samples, and for TLR2 the PCR product was 125bp on agarose gel (1%), (50volt/1hour) with a presence of 95% from total samples. The results of the present study indicate that the Toll like receptor alleles associated with risk of prostatitis.","PeriodicalId":219036,"journal":{"name":"Journal of Biotechnology & Bioinformatics Research","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127420605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the Microbiome at the Onset and End of Decomposition","authors":"M. Erquiaga, M. Fabbri, Michelle F Kowalczyk, Jessica E Thornton, Alyssa J Forrest, Erika L. Peck, Brianna M Cunningham, J. T. McClintock","doi":"10.47363/JBBR/2020(2)119","DOIUrl":"https://doi.org/10.47363/JBBR/2020(2)119","url":null,"abstract":"Decomposition is a rapidly evolving process that is dependent on various environmental factors such as climate, temperature, insect activity, large vertebrate scavenging, and microbial activity. Although other factors such as body weight and time have been evaluated, microbial activity should be considered as another major component in the decomposition process. This study was designed to investigate the microbiome and potential bacterial succession using two different DNA extraction methods, classic microbiologic techniques and 16S ribosomal sequencing at the onset and end of decomposition. Differences were observed between the bacterial phyla found on Day 1 versus Day 6. Among the various phyla, several different bacterial species were observed such as Kurthia gibsonii, K. sibirica, Staphylococcus sciuri, S. lentus, and Serratia marcescens. An interesting change in the phyla present was observed for Day 6. None of the bacterial samples collected on any of the anatomical sites were identified in the phylum Firmicutes. In fact, most of the bacteria collected from the mouth, nose, and genitals were identified in the phyla Actinobacteria and Proteobacteria. Two different bacterial species, Myroides odoratus and Leucobacter aridicollis were present in the two phyla at Day 6 that were not observed at the onset of decomposition. Likewise, many of the bacterial species present at Day 1 were not observed in Day 6. The difference in bacterial diversity observed at the onset and end of decomposition suggest that a timeline or bacterial succession could be developed that could support post-mortem interval determinations","PeriodicalId":219036,"journal":{"name":"Journal of Biotechnology & Bioinformatics Research","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130680552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary Lineages, Inherently Conserved Within the Genome of Isoetes L (Isoetaceae: Pteridophyta) Express in Heterosporangia of Isoetes Pantii Goswami & Arya: Brief Review: A Five Decades’ Study","authors":"H. Goswami","doi":"10.47363/JBBR/2020(2)121","DOIUrl":"https://doi.org/10.47363/JBBR/2020(2)121","url":null,"abstract":"Heterosporous sporangia discovered in Isoetes pantii Goswami & Arya are known as heterosporangia. Heterosporangia produce thousands of alete, monolete and trilete microspores and a few dozen megaspores. This trait is inherent within the genome of I pantii because plants collected from a location in South Gujarat also exhibit this feature. Additionally, initial collections and followup studies since 1966 have been consistently showing two types of megaspores within the heterosporangia. While microspores and megaspores develop in each and every hetrosporangium, additional unusual megaspores, variable in shape and size (180-220 micron), do not occur in all heterosporangia of all plants. These spores closely resembling fossil ancestral spores are being now termed as “Chalonospores”. Thus, a living spore genus Chalonospora is being named and defined as a spore discovered in a living plant which recall fossil ancestry of the genus but are never found in any other extant species of the genus. Since Chalanospores also originate from spore mother cells, as other microspores and megaspores do, this indicates that spore mother cells found in heterosporangia are genetically variable in expressions. Quite likely, a few megaspore mother cells must be possessing gene combinations with “revitalized relic DNA sequences”. As different types of chalonospres have been observed, variable gene combinations must be representing sporadic expressions of different lycopods of the vast geological past. Germination studies prove that these spores are endowed with different genetic make-up, as the gametophyte produced on soil culture produce unique single cell rhizoids with spiny outer layer. This apparent genomic reshuffle is related with frequently encountered chromosome breaks, translocations and irregular presence of 2n=36 –to 39 chromosomes in such plants. However, Isoetes pantii plants possess 2n=48 chromosomes with X-Y mechanism. No other species in the world flora of the genus has exhibited sex chromosomal mechanism and possessed n=12 chromosome series. With greater probability this appears plausible to imagine that naturally imposed chromosomal aberrations have rejuvenated genes which might have been regular expressions within the genome of some of the lycopods of Carboniferous and thereafter","PeriodicalId":219036,"journal":{"name":"Journal of Biotechnology & Bioinformatics Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125964047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Microbial and Anti-Oxidant Activities of Ziziphus Spina Christi, Sudanese Medicinal Plant","authors":"Mahdi Ali, A. Ahmed, Ali Abdurrahman Almakki, H. M. Hamadnalla","doi":"10.47363/JBBR/2020(2)108","DOIUrl":"https://doi.org/10.47363/JBBR/2020(2)108","url":null,"abstract":"This work is an anti-Microbial and anti-Oxidant activity of Ziziphus spina Christi based on four selected bacteria: Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus. These species were collected and prepared in Sudan. The concentrations were carried out and compared with standard control sample, the suspension of anti-microbial activities have shown different results in Fagonia cretica. The anti-oxidant activity is the ability of bioactive compounds to prevent, delay and protect against oxidant of virus substrates such as DNA and lipid materials, both in living organisms (e. g. human) and in food products. The result showed an anti-oxidant activity based on standard liquid reagent [1].","PeriodicalId":219036,"journal":{"name":"Journal of Biotechnology & Bioinformatics Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125865195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}