Shika Kiso Igakkai zasshi = Japanese journal of oral biology最新文献_第7页

Inhibitory effects of humic acid on insoluble glucan synthesis and cellular adherence of Streptococcus mutans (sobrinus) 6715. 腐植酸对变形链球菌(sobrinus) 6715不溶性葡聚糖合成及细胞粘附的抑制作用

Shika Kiso Igakkai zasshi = Japanese journal of oral biology Pub Date : 1989-06-01 DOI: 10.2330/joralbiosci1965.31.329

Y Nakamura, H Kuwashima, S Aoki, T Masuhara

引用次数: 1

Effect of stannous ion on the spontaneous transmitter release from motor nerve terminals of the frog. 亚锡离子对蛙运动神经末梢自发递质释放的影响。

Shika Kiso Igakkai zasshi = Japanese journal of oral biology Pub Date : 1989-06-01 DOI: 10.2330/joralbiosci1965.31.333

T Hattori, H Maehashi

引用次数: 0

[Histological studies of the mouse palate. Formation of the incisive suture]. 小鼠上颚的组织学研究。[锐利缝合的形成]。

Shika Kiso Igakkai zasshi = Japanese journal of oral biology Pub Date : 1989-06-01 DOI: 10.2330/joralbiosci1965.31.302

R Sukekawa, K Sato, H Kumeta, K Nisiyama, I Ito

{"title":"[Histological studies of the mouse palate. Formation of the incisive suture].","authors":"R Sukekawa, K Sato, H Kumeta, K Nisiyama, I Ito","doi":"10.2330/joralbiosci1965.31.302","DOIUrl":"https://doi.org/10.2330/joralbiosci1965.31.302","url":null,"abstract":"The morphology of the incisive suture (InS) in mice was studied using specimens stained with alizarin red S. Formation of this suture was also studied histologically with light microscopy. The InS is composed of three regions; the 1st and 2nd are short and well interdigitated regions respectively situated on the inside and the outside of the anterior palatine foramen. The 3rd region is a loose curved region situated on the outside and the nasal cavity side of the cranium. The InS surrounds the posterior region of the incisors in the upper jaw with a ring-like form. The InS is formed in four steps; cell aggregation (19 days postconception and one day postpartum), formation of bone extensions and collagen fiber bundles (4 and 7 days postpartum), modification of the orientation of these fiber bundles (14 days postpartum), and formation of the serrated suture and fiber bundles with regular orientation (21, 30 and 60 days postpartum). Furthermore, the distance between the bones in the InS was found to be from 40 to 60 microns. The morphology of the InS indicates that it functions as a strong connection between bones, and as a buffer zone. These functions of the InS seem to correspond closely with the function of the incisors in the upper jaw.","PeriodicalId":21847,"journal":{"name":"Shika Kiso Igakkai zasshi = Japanese journal of oral biology","volume":"31 3","pages":"302-7"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13662462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

[Coupling of GTP-binding protein to inositol phospholipid metabolism in chemoattractant-stimulated guinea pig peritoneal exudate macrophages]. [在化学引诱剂刺激的豚鼠腹膜渗出巨噬细胞中gtp结合蛋白与肌醇磷脂代谢的偶联]。

Shika Kiso Igakkai zasshi = Japanese journal of oral biology Pub Date : 1989-06-01 DOI: 10.2330/joralbiosci1965.31.265

M Kukita

{"title":"[Coupling of GTP-binding protein to inositol phospholipid metabolism in chemoattractant-stimulated guinea pig peritoneal exudate macrophages].","authors":"M Kukita","doi":"10.2330/joralbiosci1965.31.265","DOIUrl":"https://doi.org/10.2330/joralbiosci1965.31.265","url":null,"abstract":"The involvement of GTP-binding protein in inositol phospholipid metabolism in guinea pig peritoneal exudate macrophages stimulated with the chemoattractant N-formyl-Methionyl-Leucyl-Phenylalanine (fMLP) was examined. The GTP analog, guanosine 5'-[gamma-thio]triphosphate (GTP gamma S) caused a dose-dependent increase in the formation of inositol triphosphate in membranes of macrophages. This effect was specific for GTP and its analog. fMLP-induced inositol phospholipid turnover was markedly inhibited by the prior exposure of macrophages to 100 ng/ml of pertussis toxin (PT). Likewise, the pretreatment of macrophages with 100 ng/ml of PT evoked the inhibition of the increase in the intracellular free Ca2+ concentration and the spreading of macrophages induced by fMLP. These actions of PT were not associated with an alteration in the cellular concentration of cyclic AMP. Incubation of the membranes of macrophages with [32P]NAD and PT resulted in the ADP-ribosylation of a 41,000 Da protein. This ADP-ribosylation was diminished by the prior incubation of the membranes with 100 microM GTP gamma S plus 1 mM MgCl2, indicating that the 41,000 Da protein may be the alpha subunit of a GTP-binding protein. Moreover, there was a parallel between the time course of the ADP-ribosylation of intact macrophages by PT and the inhibition of the increase in intracellular free Ca2+ concentration as well as of the enhancement of the spreading of macrophages. These results suggest that the 41,000 Da protein, a GTP-binding protein, mediates the fMLP-stimulated inositol phospholipid metabolism.","PeriodicalId":21847,"journal":{"name":"Shika Kiso Igakkai zasshi = Japanese journal of oral biology","volume":"31 3","pages":"265-80"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13705582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytoskeleton in the apical region of mouse taste bud cells. 小鼠味蕾细胞顶端区域的细胞骨架。

Shika Kiso Igakkai zasshi = Japanese journal of oral biology Pub Date : 1989-06-01 DOI: 10.2330/joralbiosci1965.31.317

M Takeda, N Obara, Y Suzuki

引用次数: 3

Amylase activity and the isoenzymes in animals. 动物淀粉酶活性及同工酶。

Shika Kiso Igakkai zasshi = Japanese journal of oral biology Pub Date : 1989-06-01 DOI: 10.2330/joralbiosci1965.31.336

Y Uto, T Ikeno

引用次数: 1

Small angle X-ray scattering study on Lingula unguis shell. 狭舌龟壳的小角x射线散射研究。

Shika Kiso Igakkai zasshi = Japanese journal of oral biology Pub Date : 1989-06-01 DOI: 10.2330/joralbiosci1965.31.308

M Iijima, Y Moriwaki, T Gyotoku, K Hayashi, S Imura

引用次数: 4

[Production of fibroblast chemotactic factors from root canal bacteria]. [根管细菌产生成纤维细胞趋化因子]。

Shika Kiso Igakkai zasshi = Japanese journal of oral biology Pub Date : 1989-06-01 DOI: 10.2330/joralbiosci1965.31.227

H Fujimoto

{"title":"[Production of fibroblast chemotactic factors from root canal bacteria].","authors":"H Fujimoto","doi":"10.2330/joralbiosci1965.31.227","DOIUrl":"https://doi.org/10.2330/joralbiosci1965.31.227","url":null,"abstract":"It is well known that periapical pathosis is one of endogenous infections caused by indigenous bacteria in the oral cavity. Therefore, interaction of host and parasite factors affect the progress of the lesion. In host factors, fibroblasts migrate chemotactically, proliferate and constitute new connective tissues at a late stage of the inflammation process. All of chemoattractants for fibroblasts previously reported are derived from the host. In this study, fibroblast chemotactic activities in bacteria isolated from chronic periapical pathosis cases were examined. Fibroblast chemotactic activity was measured by the membrane filter method using cultured guinea pig dermal fibroblasts. Fibroblast migration was activated by bacterial supernatants of 4 species among 45 species tested. This indicates the possibility that these bacterial factors as well as host derivatives such as fibronectin, lymphokine, collagen-, elastin- and platelet-derived factors, may exert an influence on the process of periapical pathosis. The supernatant from Succinivibrio dextrinosolvens showed the most intensive chemotactic activity, which were separated into two fractions by Sephacryl S-300. The active fraction having a lower molecular weight (Mw. ca. 280K) did not absorb on DEAE-Sepharose CL-6B and this activity was resistant to heat and proteolytic enzymes.","PeriodicalId":21847,"journal":{"name":"Shika Kiso Igakkai zasshi = Japanese journal of oral biology","volume":"31 3","pages":"227-39"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2330/joralbiosci1965.31.227","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13665542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Establishment of acinar cells from human salivary gland tissue by transformation of SV40]. [转化SV40构建人唾液腺组织腺泡细胞]。

Shika Kiso Igakkai zasshi = Japanese journal of oral biology Pub Date : 1989-06-01 DOI: 10.2330/joralbiosci1965.31.248

S Kiyonobu

{"title":"[Establishment of acinar cells from human salivary gland tissue by transformation of SV40].","authors":"S Kiyonobu","doi":"10.2330/joralbiosci1965.31.248","DOIUrl":"https://doi.org/10.2330/joralbiosci1965.31.248","url":null,"abstract":"A piece of minor salivary gland tissue obtained from the lip of a 16 years old female was minced to about 3 mm3 by fine pincettes and cultured with 10% FCS containing MEM supplemented with EGF (10 ng/ml), fungizon (10 mcg/ml) and kanamycin (60 mcg/ml) in a 5% CO2 incubator. Many small bubbles of saliva were found on the surface of the fragments after 3 to 4 days of incubation and outgrowth of cells from the fragments was observed from 7 days of incubation. Monolayer cells of the outgrowth were trypsinized and passaged with fresh culture medium. At the 8th passage, monolayer cells were infected with SV40 at moi 100PFU/cell. After 18 hour-incubation, the suspension of the infected cells was incubated at densities of 10(4) and 10(3) cells/dish within 0.33% agar containing culture medium. Transformed colonies were picked up from soft agar medium and 3 of the 28 colonies were identified as being acinar cells of the salivary gland, since secretory granules and mucosubstances were specifically proved in the cytoplasm of these cells after 2 to 4 days of incubation. One of the typical clone cells was named HA-16 cells. However, the appearances of the secretory granules and mucins in the cytoplasm of the HA-16 cells depended on the cellular growth cycle, i.e. secretory granules and mucins were not found in the growing cells (G1-S-G2-M phase) but many secretory granules and mucins could be recognized in the non-dividing cells (G0 phase).(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":21847,"journal":{"name":"Shika Kiso Igakkai zasshi = Japanese journal of oral biology","volume":"31 3","pages":"248-56"},"PeriodicalIF":0.0,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13705580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological study of the cytoskeleton of endothelial cells in vitro. Effects of heparin and protamine. 内皮细胞体外细胞骨架的形态学研究。肝素和鱼精蛋白的作用。

Shika Kiso Igakkai zasshi = Japanese journal of oral biology Pub Date : 1989-04-01 DOI: 10.2330/joralbiosci1965.31.184

N Aita

{"title":"Morphological study of the cytoskeleton of endothelial cells in vitro. Effects of heparin and protamine.","authors":"N Aita","doi":"10.2330/joralbiosci1965.31.184","DOIUrl":"https://doi.org/10.2330/joralbiosci1965.31.184","url":null,"abstract":"The relation between the cytoskeleton and multiplication of cultured endothelial cells was observed immunocytochemically and electron microscopically. Heparin and protamine were used to control the multiplication of the cells. Cytochalasin D, a microfilament synthesis inhibitor, was also used, and its effect on cytoskeletal morphology was compared with the effect of heparin or protamine. In order to quantify changes in microfilaments, the ratio of the length of microfilaments to that of intermediate filaments (M/I ratio) was measured by transmission electron microscopy of the whole mounted cells. The addition of heparin to the culture medium not only enhanced multiplication of the endothelial cells, but also induced the outgrowth of many pseudopodia. In association with these changes, microfilaments became sparse, and stress fibers became short. The addition of protamine to the culture medium suppressed multiplication of endothelial cells and made them spherical. The thickening of the cytoplasmic layer increased the apparent density of the cytoskeleton, but the M/I ratio remained unchanged. Cytochalasin D made the endothelial cells spherical, and at the same time stress fibers disappeared, and microfilaments became sparse. The M/I ratio was smaller than that of the others. Protamine may inactivate protein which forms bridges to connect microfilaments or which binds microfilaments and the cell membrane.","PeriodicalId":21847,"journal":{"name":"Shika Kiso Igakkai zasshi = Japanese journal of oral biology","volume":"31 2","pages":"184-97"},"PeriodicalIF":0.0,"publicationDate":"1989-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13665537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1