Small angle X-ray scattering study on Lingula unguis shell.

Abstract

Lingula unguis shell yields a diffuse small angle X-ray scattering which is caused mainly by the scattering from particles of apatite. In this study, the distribution of particles smaller than 500A was analyzed using the small angle X-ray scattering technique. The non-linear Guinier plot indicates that the shell contains various sizes of apatite particles ranging about 20-460A. The small granules with size about 20-160A in diameter are located in the marginal part of the shell and progressively larger granules are observed internally towards the central part. The scattering is anisotropic in the lateral and central part, where the apatite crystals highly ordered. The combined analysis of wide and small angle diffraction indicates that the particles are elongated in the c-axis direction and, furthermore, their long axes are arranged almost parallel to the direction of growth in these parts. This suggests that small and isotropic particles are formed in the early stage of mineralization and larger ellipsoidal shaped particles are formed in the later stage of mineralization.