[在化学引诱剂刺激的豚鼠腹膜渗出巨噬细胞中gtp结合蛋白与肌醇磷脂代谢的偶联]。

M Kukita
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引用次数: 0

摘要

研究了gtp结合蛋白在化学引诱剂n -甲酰基-蛋氨酸-酰-苯丙氨酸(fMLP)刺激下豚鼠腹腔渗出巨噬细胞肌醇磷脂代谢中的作用。GTP类似物鸟苷5′-[γ -硫]三磷酸(GTP γ S)引起巨噬细胞膜中肌醇三磷酸形成的剂量依赖性增加。这种效应是GTP及其类似物所特有的。巨噬细胞暴露于100 ng/ml百日咳毒素(PT)后,fmlp诱导的肌醇磷脂转化被显著抑制。同样,100 ng/ml PT预处理巨噬细胞可抑制fMLP诱导的细胞内游离Ca2+浓度升高和巨噬细胞的扩散。PT的这些作用与环AMP细胞浓度的改变无关。[32P]NAD和PT对巨噬细胞膜的孵育导致41,000 Da蛋白的adp核糖基化。这种adp核糖基化通过预先用100微米GTP γ S和1毫米MgCl2孵育膜而减弱,表明41,000 Da蛋白可能是GTP结合蛋白的α亚基。此外,PT对完整巨噬细胞adp -核糖基化的时间过程与抑制细胞内游离Ca2+浓度的增加以及增强巨噬细胞的扩散具有平行关系。这些结果表明,41,000 Da蛋白,一种gtp结合蛋白,介导了fmlp刺激的肌醇磷脂代谢。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Coupling of GTP-binding protein to inositol phospholipid metabolism in chemoattractant-stimulated guinea pig peritoneal exudate macrophages].

The involvement of GTP-binding protein in inositol phospholipid metabolism in guinea pig peritoneal exudate macrophages stimulated with the chemoattractant N-formyl-Methionyl-Leucyl-Phenylalanine (fMLP) was examined. The GTP analog, guanosine 5'-[gamma-thio]triphosphate (GTP gamma S) caused a dose-dependent increase in the formation of inositol triphosphate in membranes of macrophages. This effect was specific for GTP and its analog. fMLP-induced inositol phospholipid turnover was markedly inhibited by the prior exposure of macrophages to 100 ng/ml of pertussis toxin (PT). Likewise, the pretreatment of macrophages with 100 ng/ml of PT evoked the inhibition of the increase in the intracellular free Ca2+ concentration and the spreading of macrophages induced by fMLP. These actions of PT were not associated with an alteration in the cellular concentration of cyclic AMP. Incubation of the membranes of macrophages with [32P]NAD and PT resulted in the ADP-ribosylation of a 41,000 Da protein. This ADP-ribosylation was diminished by the prior incubation of the membranes with 100 microM GTP gamma S plus 1 mM MgCl2, indicating that the 41,000 Da protein may be the alpha subunit of a GTP-binding protein. Moreover, there was a parallel between the time course of the ADP-ribosylation of intact macrophages by PT and the inhibition of the increase in intracellular free Ca2+ concentration as well as of the enhancement of the spreading of macrophages. These results suggest that the 41,000 Da protein, a GTP-binding protein, mediates the fMLP-stimulated inositol phospholipid metabolism.

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