ScienceRise: Pharmaceutical Science最新文献

{"title":"Utilization of water extract of yellow potato (Solanum tuberosum L.) in hydrogel eye mask as anti-aging formulation","authors":"Mawalia Mawalia, Julia Reveny, U. Harahap","doi":"10.15587/2519-4852.2022.261641","DOIUrl":"https://doi.org/10.15587/2519-4852.2022.261641","url":null,"abstract":"The thinness and sensitivity of the skin in the area under the eyes cause the skin in this area to easily show signs of aging. Hydrogel masks contain quite a lot of water, so during use this mask will moisturize the skin longer. A hydrogel eye mask containing yellow potato tuber water extract is used for the prevention of premature aging, especially under the eyes.\u0000The purpose of this study was to determine whether hydrogel eye mask preparations containing water extract of yellow potato tubers can provide an anti-aging effect.\u0000Material and methods: The yellow potato tuber water extract was screened for phytochemicals, then formulated into a hydrogel eye mask with a concentration of 0.25 %, 0.5 %, and 1 %. Evaluation of the hydrogel eye mask preparation includes organoleptic, weight, thickness, pH test, tensile test, swelling power, shrinkage, stability, cycling test, hedonic test, and anti-aging. Anti-aging parameters measured include moisture, pores, blemishes, and wrinkles. The treatment was carried out for four weeks by applying the mask twice a week.\u0000Results: The results showed that all hydrogel eye mask formulas were stable during storage and cycling tests. All formulas meet pH values, shrinkage, elasticity, swellability, and irritation tests. The hedonic test on volunteers shows the most preferred concentration of 1 %. The results of the anti-aging effectiveness test of the best hydrogel eye mask preparation is a concentration of 1 % with an increase in humidity of 27 %, a decrease in pores of 35.8 %, blemishes of 40 %, and wrinkles of 37.6\u0000Conclusion: That the different concentrations of each formula showed different anti-aging activities and the best formula was 1 % with moisture values of 27 %, pore values of 35.8 %, blemishes of 40 %, and wrinkles values of 37.6 %, which indicated anti-aging activity","PeriodicalId":21674,"journal":{"name":"ScienceRise: Pharmaceutical Science","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83110139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of algorithm of the prevention complex of inflammatory processes in the oral cavity in metabolic syndrome","authors":"L. Kravchenko, Olen Appelhans, A. Poliakov, L. Unhurian, Oleksandr Pasechnyk, M. Rozumenko, Y. Ivanova, V. Rozumenko","doi":"10.15587/2519-4852.2022.257527","DOIUrl":"https://doi.org/10.15587/2519-4852.2022.257527","url":null,"abstract":"The aim of the work was to evaluate in the experiment the effectiveness of the developed treatment-and-prophylactic complex for the prevention of periodontal tissue disorders under metabolic syndrome simulation.\u0000Materials and methods: The study of biochemical and immunological changes in the blood serum, liver and gingival tissue was performed during simulation on the metabolic syndrome of alimentary genesis on Wistar rats, all animals were divided into 5 groups: 1) intact, 2) with simulated metabolic syndrome, 3) in a week after the start of MS simulation 5 times a week in the morning perorally administered a “Capillaroprotect” aqueous solution (bioflavonoid, antioxidant) produced by “Ekosvit Oil” (Ukraine) at a rate of 135 mg/kg, 4) under similar conditions receiving the preparation based on the dihydroquercetin were locally applied on gums a new dental elixir based on bee products and adaptogens of plant origin by o,5 ml/rat with a tampon, which has held for 5–7 minutes, 5) during MS simulation from the second week were used the preparation based on the dihydroquercetin, the dental elixir topically on gums and physiotherapy.\u0000Results: Under conditions of experimental MS simulation with a diet rich in saturated fats and carbohydrate there are systemic disorders in the body: reduced nonspecific antimicrobial protection, increased microbial contamination, intensification of lipid peroxidation, the development of inflammation and hepatotoxicity. Prophylactic administration of the proposed dihydroquercetin preparation to animals in the process of simulation of MS significantly inhibits the established disorders, positively affecting the biochemical parameters of the blood serum, liver tissue, periodontium, reducing triglycerides, total cholesterol, glucose level, restoring the state of non-specific resistance, lipid metabolism, preventing inflammation and hepatosis, as well as contamination with pathogenic microflora.\u0000Conclusion. The proposed treatment-and-prophylactic complex, which includes the dihydroquercetin preparation, used per os in combination with local therapy of periodontal tissues with a tooth elixir based on propolis and biologically active substances of plant origin adaptogens with ultraphonophoresis under induced metabolic syndrome significantly removed the negative effects of its most important components","PeriodicalId":21674,"journal":{"name":"ScienceRise: Pharmaceutical Science","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89962900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition of essential oils from flowers of veronica Longifolia L., Veronica Incana L. and Veronica Spicata L.","authors":"A. Kovaleva, A. Osmachko, Т. Ilina, O. Goryacha, Ludmila Omelyanchik, A. Grytsyk, O. Koshovyi","doi":"10.15587/2519-4852.2022.263735","DOIUrl":"https://doi.org/10.15587/2519-4852.2022.263735","url":null,"abstract":"In the Ukrainian flora, species of Veronica L. genus (Plantaginaceae Juss.) are classified into 8 sections. The phytochemical research into secondary metabolites of Veronica L. genus most related to the study of phenolic compounds and iridoids, while terponoids of these species need further research. The chemical profiles of V. longifolia L., V. incana L. and V. spicata L. of Ukrainian flora are poorly studied. Phenolic acids, hydroxycinnamic acids, coumarins, flavonoids, tannins, iridoids, saponins, amino acids and organic acids have been reported for these species. Herbs harvested during the flowering stage are often used in the pharmaceutical industry, so the research into chemical composition of essential oils from Veronica species flowers are urgent. \u0000The aim of this study was a comparative GC/MS study of the chemical composition of essential oils from V. longifolia L., V. incana L. and V. spicata L. flowers of Ukrainian flora. \u0000Materials and methods. The objects of the research were flowers of Veronica spp. of Pseudolysimachium W.D.J. Koch section, namely V. longifolia L., V. incana L. and V. spicata L., harvested in the Botanical Garden of V. N. Karazin Kharkiv National University. The study of the chemical composition of essential oils was carried out by chromatography mass spectrometry on a 6890N MSD/DS Agilent Technologies chromatograph (USA) with a 5973N mass spectrometric detector. The components of essential oils were identified by comparison of the retention indices and mass spectra of phytochemicals in the studied essential oils with the data of NIST02 mass spectral library. The quantification of substances in the raw materials was carried out in comparison with a standard sample of menthol. \u0000Results. As a result, 72 compounds were detected and quantified. The total content of essential oil in V. longifolia L. flowers was 0.17 % (39 components), the following compounds dominated: benzoacetaldehyde – 8.05, squalene – 5.17, palmitic acid – 15.73, butyl phthalate – 7.18. The total content of essential oil in V. incana L. flowers was 0.15 % (43 components), the following compounds prevailed: squalene 20.47, fatty acids, namely palmitic – 26.88, palmitoleic – 17.15, oleic – 11.61. The total content of the essential oil in V. spicata L. flowers was 0.11 % (43 components), the following compounds dominated: squalene – 5.53, fatty acids: palmitic – 22.78, linoleic – 6.72, carbohydrates: heptacosan – 12.27, hexacosan – 7.45. Among the identified compounds, mono-, norsesqui-, sesqui-, di- and triterpenoids, their oxidation products (aromatic compounds, aldehydes and alcohols, ketones), fatty acids, hydrocarbons and their derivatives were detected. \u0000Conclusions. The chemical composition of essential oils from flowers of V. longifolia L., V. incana L. and V. spicata L. from Ukrainian flora was first studied by means of chromatography mass spectrometry. The yield of essential oil from V. longifolia L. flowers is higher (0.17 %) compared to thos","PeriodicalId":21674,"journal":{"name":"ScienceRise: Pharmaceutical Science","volume":"104 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76864030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring of indicators of physical availibility and socio-economic affordability of metformin hydrochloride medicines","authors":"Oksana Ryshchenko, D. Lytkin, I. Podolsky, A. Volkova, I. Vladymyrova","doi":"10.15587/2519-4852.2022.263675","DOIUrl":"https://doi.org/10.15587/2519-4852.2022.263675","url":null,"abstract":"The aim of the study was to monitor the physical availability and socio-economic affordability of metformin hydrochloride medicines, which are used for the treatment of type II diabetes mellitus. \u0000Materials and methods. Medical and technological documentation on the standardization of medical care for patients with type II diabetes, data from the State Register of Medicinal Products of Ukraine, software complex “Apteka” of the company “Morion” and data from the pharmaceutical market research analytical company “Pharmstandard” of the company “Morion” were used as research materials to determine indicators of physical availability and socio-economic affordability of metformin HCL medicines for the treatment of type II diabetes. Documentary, analytical methods, the method of marketing research and the method of logical summarization of data were used during the research. \u0000Results. According to the results of the analysis of the medical and technological documentation on the standardization of medical care for diabetes mellitus (DM) type II and the State Register of Medicines of Ukraine, a number of characteristics were formed for Metformin HCL pharmaceuticals, namely: tablets or film-coated tablets in a dose of 500 mg, which are manufactured: Ukrainian manufacturers (full cycle of production); Ukrainian manufacturers of tablets in bulk (primary and secondary packaging) and foreign manufacturers. The specified characteristics became the basis for the further selection of several metformin HCL medicines in terms of determining their indicators of physical availability and socio-economic affordability. When determining the physical availability of metformin HCL medications, it was established that Ukrainian-made pharmaceuticals (full production cycle) are presented in the wholesale chain of the pharmaceutical market in full. According to the socio-economic indicator of the solvency adequacy of payment capacity, among the metformin HCL pharmaceuticals, the most economically justified are the Ukrainian-made pharmaceuticals, which are made from tablets in bulk according to the full production cycle. Only 1 medicine was singled out among foreign-made ones, which has the lowest indicator of solvency adequacy for various categories of the population. \u0000Conclusions. Monitoring of indicators of physical availability and socio-economic affordability of metformin HCL medicines showed that the most economically justified for able-bodied people and people of retirement age is the use of the Ukrainian-made pharmaceuticals (full cycle and production from tablets in bulk) for the treatment of type II DM)","PeriodicalId":21674,"journal":{"name":"ScienceRise: Pharmaceutical Science","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74340005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of quality indicators of promising plant raw materials - underground organs of Rumex confertus willd","authors":"T. Oproshanska, O. Khvorost, I. Batiuchenko, L. Ivanauskas, Anastasiia Belikova","doi":"10.15587/2519-4852.2022.259583","DOIUrl":"https://doi.org/10.15587/2519-4852.2022.259583","url":null,"abstract":"The aim is to study the morphological and anatomical structure of the underground organs of R. confertus and to establish the type of medicinal plant raw materials, to determine a number of its indicators of quality and quantitative content of some groups of biologically active substances using modern methods of analysis. \u0000Materials and methods. It was used air-dry and freshly collected raw materials and a microscope Delta optic BioLight 300 (Poland) to study the macro- and microscopic characteristics of the plant raw materials. Determination of amount of hydroxycinnamic acids and total polyphenols was determined spectrophotometrically according to monograph «Nettle leaf» and the method 2.8.14 of the State Pharmacopoeia of Ukraine 2.0 (on a spectrophotometer Optizen POP (Korea)). The study of the component composition of hydroxycinnamic acids was performed by HPLC. \u0000Results. As a result of research of plant raw materials it was established that the underground organs of Rumex confertus are the roots of annual plants and the rhizomes and roots of two or three annual plants. Diagnostic features: morphological (for the root and rhizome - the nature of the surface and fracture) and anatomical (for the rhizome - aerenchyma in the cortex, the presence and location of scleroids and sclerenchyma in the plant raw materials of two or three annual plants, the presence in the cells of the cortex and pith parenchyma simple starch grains, druses and cells with yellow content in freshly harvested plant raw materials; for the root - the colour of peridermal cells, the degree of development of pith rays, the remainder of the primary xylem; a distinctive feature of the annual root from two or three annuals is the absence of scleroids). It was determined the borderline boundaries of indicators in the series of plant raw materials: loss on drying (not more than 13. 5 %), total ash (not more than 11 %), extractable matter (not less than 33 %) and the quantitative content of amount of hydroxycinnamic acids (not less than 1.3 %) and total polyphenols (not less than 3.5 %). It was identified chlorogenic and neochlorogenic acids. \u0000Conclusions. It was determined the type of underground organs of Rumex confertus: annuals had only roots (tap root systems), from the second to the third year of life the plants have both rhizomes and tap root system. It was established their morphological and anatomical diagnostic features and determined numerical indicators and the quantitative content of the amount of hydroxycinnamic acids and total polyphenols with using modern methods of analysis and chlorogenic and neochlorogenic acids were identified. The obtained data will be used in further research, including the development of the draft monograph of the State Pharmacopoeia of Ukraine 2.0 or the draft methods of quality control of medicinal plant raw materials Rumex confertus and the creation of herbal medicines","PeriodicalId":21674,"journal":{"name":"ScienceRise: Pharmaceutical Science","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81105260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research of the causes and consequences of mobing in the lablor team of a pharmacy establishment","authors":"N. Andrienko, R. Sahaidak-Nikitiuk, N. Demchenko, D. Zoidze, V. Nikitiuk, N. Zakharko","doi":"10.15587/2519-4852.2022.260355","DOIUrl":"https://doi.org/10.15587/2519-4852.2022.260355","url":null,"abstract":"The aim of the article is to analyze the causes and consequences of mobbing, which occurs in the staff of the pharmacy. \u0000Materials and methods. The methods used in the study include methods of theoretical generalization, analysis and synthesis, content analysis, expert survey, correlation analysis. \u0000Results. The situation in pharmacies regarding the presence of mobbing in the workforce has been studied. 31 % of respondents in their professional activities encountered mobbing, and 47 % said that such phenomena were quite common, and 44 % often encountered insults and intimidation. The reasons for mobbing in the staff of the pharmacy and the its reasons in the staff of the pharmacy by the employee who is subjected to mobbing are formulated. The distribution of status in the workforce (9.9±0.1 points), envy of a younger or more successful colleague (A2) (9.4±0.15 points), distribution of statuses in the labor collective (A3) (9.3±0.1 points), low level of communication skills (9.2±0.1 points) and unhealthy socio-psychological climate in the workforce (9.1±0.2 points), age (9.0±0.3 points). Statistically significant relationships between the attitude of pharmacy staff to mobbing and age were identified; general work experience and work experience in a pharmacy; gender, managerial, professional, and interpersonal tolerance and its identification-group component and commitment to the pharmacy Negative attitude to mobbing is manifested in the most loyal to the pharmacy staff. The manifestations of mobbing in the staff of the pharmacy were studied, namely: boycott of the employee (9.1±0.15 points), constant and often unfounded criticism (8.4±0.15 points), unfair and offensive evaluation of work (7.9±0.17 points), etc. The consequences of mobbing in the workforce of the pharmacy were determined, which include the creation of an unhealthy socio-psychological climate in the workforce, high staff turnover, reduced productivity, disruption of communication in the workforce, disruption of communication between pharmacist and pharmacy visitor, regular visitors, deterioration of the reputation of the pharmacy, etc. \u0000Conclusions. The causes of mobbing in the pharmacy have been identified. In the perspective of the identified problems of mobbing in the pharmacy, its consequences for the pharmacy are determined","PeriodicalId":21674,"journal":{"name":"ScienceRise: Pharmaceutical Science","volume":"302 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74184086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The management of premenstrual syndrome: results of a questionnaire survey of women in Lviv pharmacies, Ukraine","authors":"Kh. I. Makukh, O. Horodnycha, O. Nepyivoda","doi":"10.15587/2519-4852.2022.260186","DOIUrl":"https://doi.org/10.15587/2519-4852.2022.260186","url":null,"abstract":"The aim. To establish the prevalence of PMS symptoms and evaluate the medication management of this disorder in Lviv. \u0000Materials and methods. The objects of the study were: scientific publications related to the problem of PMS; the results (n=105) of the survey. Methods applied: systematization, generalization, comparison, questionnaire. The statistical analyses (descriptive statistics, univariable analysis using simple logistic regression, multiple logistic regression) were performed with SPSS Trial. \u0000Results. The prevalence of PMS among the surveyed was high (83,8 %). The most common complaints are abdominal and/or low back pain (46,6 %) and increased irritability/aggression (43,2 %). Almost half (46,6 %) of women who experienced PMS consult a pharmacist, and only 23,9 % seek medical advice. At the same time, 87,5 % (n=77) of women (among those suffering from PMS) use medicines to alleviate/eliminate the symptoms of PMS. Nonsteroidal anti-inflammatory drugs (77,6 %), sedatives (36,8 %), and complex herbal remedies that affect the genital system (18,4 %) are the most common drugs for PMS. \u0000Choosing the way of PMS management, 44,8 % of women would prefer herbal medicines to synthetic ones. Both previous using of synthetic drugs for PMS and adverse drug reactions to synthetic drugs have a statistically significant contribution to a positive attitude towards herbal remedies (p=0,004 and p=0,026, respectively). \u0000Conclusion. PMS is a common medical and social issue. Achieving effective and safe medication management of PMS requires the joint participation of a physician, pharmacist, and the patient in terms of compliance and lifestyle adjustments","PeriodicalId":21674,"journal":{"name":"ScienceRise: Pharmaceutical Science","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86501917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research in components of essential oils from flowers and leaves of the genus Alchemilla L. species","authors":"N. Dubel, A. Grytsyk, L. Grytsyk, O. Koshovyi, A. Kovaleva","doi":"10.15587/2519-4852.2022.259059","DOIUrl":"https://doi.org/10.15587/2519-4852.2022.259059","url":null,"abstract":"The genus Alchemilla L. has about 1000 species in the world flora, of which 35 grow in Ukraine. The most common in Ukraine are A. flabellata Bus., A. phegophila Juz. and A. subrenata Bus. Despite the widespread distribution of species of the genus Alchemilla L. and the publication of a monograph on the herb of the collective species - Herba Alchemillae (Alchemiilla vulgaris L. sensu latiore) in the State Pharmacopoeia of Ukraine, the chemical composition, and pharmacological properties of species of the genus Alchemilla L. are insufficiently studied, therefore, it is advisable to study the chemical composition of the most common species of flora of Ukraine for their introduction into medical and pharmaceutical practice. \u0000The aim of the work was to study the component composition and quantitative content of essential oils of flowers and leaves of some species of the genus Alchemilla L. \u0000Materials and methods. Flowers and leaves of Alchemilla. flabellata Bus., Alchemilla phegophila Juz. and Alchemilla subrenata Bus. harvested in the Ivano-Frankivsk region in 2020 - 2021. \u0000The component composition and quantitative content of essential oils were determined by chromato-mass spectrometric method. The compounds were identified by comparing the obtained mass spectra of the chromatographic peak with the mass spectra of the reference compounds and based on comparison with the spectra of the database. Quantitative determination of the content of substances in the raw material was performed in comparison with a standard sample of menthol. \u0000Results. The essential oil of flowers and leaves of Alchemilla. flabellata Bus., Alchemilla phegophila Juz. and Alchemilla subrenata Bus were obtained. It was found that the highest content of essential oils was characterized by Alchemilla. flabellata Bus. flowers (16884.6 mg / kg), and the least essential oil was contained in the leaves of Alchemilla Phegophila Juz. (4895.5 mg / kg). As a result of studying the component composition of essential oils of flowers and leaves of Alchemilla flabellata Bus., Alchemilla Phegophila Juz. and Alchemilla subrenata Bus. 48, 51 and 47 compounds were identified, of which 44, 48 and 43 were identified, respectively. 31 components of essential oil were common in the studied samples of raw materials. \u0000Conclusions. Due to the component composition of the essential oil of flowers and leaves of Alchemilla. flabellata Bus., Alchemilla phegophila Juz. and Alchemilla subrenata Bus., as well as considering the known pharmacological activity of its components, it is advisable to conduct further pharmacological studies of raw materials of the genus Alchemilla L. to study their antibacterial, anti-inflammatory and antitumor properties","PeriodicalId":21674,"journal":{"name":"ScienceRise: Pharmaceutical Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90767272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemicals profile and in-vitro antidiabetic potentials of fractionated extracts of Entada Africana and Leptadenia Hastata","authors":"E. Adewole, Bashira Yusuf, Ogola-Emma Ebitimitula, A. Ojo, D. Adewumi, O. Oludoro, Hope Akinwale, Abiodun Adejori, B. Oyinloye","doi":"10.15587/2519-4852.2022.255744","DOIUrl":"https://doi.org/10.15587/2519-4852.2022.255744","url":null,"abstract":"Aim: To confirm their use in the management of diabetes and to determine the numerous phytochemicals present that may be connected to the active performance of the plants, the fractionated extracts of Leptadenia hastata and Entada africana were subjected to an in vitro experiment.\u0000Material and methods: The plant leaves were dried, pulverized with a Sumeet CM/L 2128945 grinder, the particle size was 45.85 μm and extracted with methanol. The crude extracts were fractionated using a 30×8 cm diameter column and 60g of silica gel 60 F254 grade, using methanol as eluent and fractions were concentrated using a rotary evaporator, the fractionated extracts were run on thin layer Chromatographic plate (TLC) and their retardation factors (RF) were determined. Fractions of similar RF were pulled together and spotted again using TLC plate and the final (RF) were calculated. The crude extracts were quantified for the content of phytochemicals and the phytochemicals present in the fractionated extracts (LH1 and EA2) were identified using HPLC-UV detector. The extracts (LH1 and EA2) were tested for antidiabetic potentials using α -glucosidase and α-amylase enzymes in an in-vitro antidiabetic assay.\u0000Results: The yields of the fractionated extracts were 10.0 mg (Leptadenia hastata) and 11.5.0 mg (Entada Africana) and designated as LH1 and EA2, the RF for LH1 and EA2 were 0.75±0.01 and 0.77±0.03 respectively. The maximum amount of alkaloid was found in E. Africana (14.50±0.25 mg/g), while tannin was not found in L. Hastata. In the portion of L. Hastata (LH1), thirteen phytochemicals were discovered and out of these three were alkaloids. Thirteen phytochemicals were found in the E. Africana fraction (EA2), with eight of them being alkaloids and flavonoids. When compared to the usual acarbose, the plants' anti-diabetic properties were superior. EA2 had EC50 of 0.950.17 g/ml (α-amylase) and 0.970.41 g/ml (α-glucosidase), while LH1 had EC50 of 1.00±0.11 g/ml (α-amylase) and 0.90±0.35 g/ml (α-glucosidase). The presence of the detected phytochemicals may be linked to the active qualities of the plants' leaves.\u0000Conclusion: The phytochemical profile of fractionated extracts classified as flavonoids and alkaloids are stated to be antidiabetic agents, and this has proved that the researched plants have antidiabetic potential","PeriodicalId":21674,"journal":{"name":"ScienceRise: Pharmaceutical Science","volume":"107 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83710666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a method for determining the morpholinium thiazotate using more economic and green GC/MS assay with an fid detector","authors":"Anastasiia Belikova, Anna Materienko, L. Sidorenko, V. Chornyi, I. Korzh, Luidmula Kucherenko, A. Kotvitska, Deividas Burdulis, V. Georgiyants","doi":"10.15587/2519-4852.2022.259879","DOIUrl":"https://doi.org/10.15587/2519-4852.2022.259879","url":null,"abstract":"One of the main steps in the pharmaceutical development of drugs is the choice of quality control methods. The correctness of the method must be confirmed by validation. In addition, manufacturers take into account various economic and environmental factors. It is especially important to determine the above aspects for domestic and promising drugs, such as the morpholinium thiazotate.\u0000The aim. During the development of methods for the routine analysis of medicinal products, attention should be paid to efficiency of analysis, budget, as well as their impact to the environment. Because of this reason, not only new methods for routine analysis should be developed. It is important this methods must be environmentally-friendly and cost-efficient.\u0000Materials and methods. The determination of the morpholinium thiazotate was carried out by HPLC using the SunFire C18 (150 mm × 4,6 mm, 5,0 μm) and gas chromatography with a flame ionization detector using the Rxi-5 ms (30 m long, 0.25 mm outer diameter and 0.25 μm liquid stationary phase thickness).\u0000Results. Various chromatographic methods for the routine quantitative analysis of morpholinium thiazotate were developed. The most suitable conditions for sample preparation were established. Proposed methods were compared to find the most ecological and economic.\u0000Conclusions. Proposed methods were accurate and reliable. However, an environmental impact assessment showed that GC-FID is a more environmentally friendly and economical method of analysis. Using 12 Principles of green analytical chemistry, the overall “analytical GREEnness (AGREE)” scale for the proposed analytical approach was computed 0.72, showing the good greener nature of the proposed analytical approach","PeriodicalId":21674,"journal":{"name":"ScienceRise: Pharmaceutical Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80098226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}