有前途的植物原料——山楂地下器官质量指标的建立

Q3 Pharmacology, Toxicology and Pharmaceutics
T. Oproshanska, O. Khvorost, I. Batiuchenko, L. Ivanauskas, Anastasiia Belikova
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引用次数: 1

摘要

目的是研究黄菖蒲地下器官的形态解剖结构,确定药用植物原料的种类,用现代分析方法确定其若干质量指标和若干类生物活性物质的定量含量。材料和方法。采用风干和新鲜采集的原料和Delta optic BioLight 300(波兰)显微镜研究植物原料的宏观和微观特征。分光光度法测定羟基肉桂酸和总多酚的含量,方法参照《荨麻叶》专著,乌克兰国家药典2.0版2.8.14(分光光度仪Optizen POP(韩国))。采用高效液相色谱法对羟基肉桂酸的组成进行了研究。结果。通过对植物原料的研究,确定其地下器官为一年生植物的根和二、三一年生植物的根茎和根。诊断特征:形态学(根和根茎-表面和断裂的性质)和解剖学(根和根茎-皮质的空气组织),二、三年生植物的植物原料中存在硬核和厚壁组织及其位置,新鲜收获的植物原料中存在皮层和髓薄壁组织的细胞中存在简单的淀粉粒、颗粒和含黄色的细胞;对于根-表皮细胞的颜色,髓射线的发育程度,初生木质部的剩余部分;一年生植物根与二年生或三年生植物根的一个显著特征是没有硬核。确定了植物原料系列指标的临界边界:干燥损失(不大于13;5%)、总灰分(不多于11%)、可萃取物(不少于33%)及羟基肉桂酸(不少于1.3%)及总多酚(不少于3.5%)的定量含量。鉴定出绿原酸和新绿原酸。结论。结果表明:一年生植物只有根(抽根系统),生后2 ~ 3年既有根茎又有抽根系统。建立了其形态解剖诊断特征,采用现代分析方法测定了羟基肉桂酸和总多酚含量的数值指标和定量含量,鉴定了绿原酸和新绿原酸。获得的数据将用于进一步的研究,包括制定乌克兰国家药典2.0的专著草案或药用植物原料Rumex confertus的质量控制方法草案和草药的创建
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Establishment of quality indicators of promising plant raw materials - underground organs of Rumex confertus willd
The aim is to study the morphological and anatomical structure of the underground organs of R. confertus and to establish the type of medicinal plant raw materials, to determine a number of its indicators of quality and quantitative content of some groups of biologically active substances using modern methods of analysis. Materials and methods. It was used air-dry and freshly collected raw materials and a microscope Delta optic BioLight 300 (Poland) to study the macro- and microscopic characteristics of the plant raw materials. Determination of amount of hydroxycinnamic acids and total polyphenols was determined spectrophotometrically according to monograph «Nettle leaf» and the method 2.8.14 of the State Pharmacopoeia of Ukraine 2.0 (on a spectrophotometer Optizen POP (Korea)). The study of the component composition of hydroxycinnamic acids was performed by HPLC. Results. As a result of research of plant raw materials it was established that the underground organs of Rumex confertus are the roots of annual plants and the rhizomes and roots of two or three annual plants. Diagnostic features: morphological (for the root and rhizome - the nature of the surface and fracture) and anatomical (for the rhizome - aerenchyma in the cortex, the presence and location of scleroids and sclerenchyma in the plant raw materials of two or three annual plants, the presence in the cells of the cortex and pith parenchyma simple starch grains, druses and cells with yellow content in freshly harvested plant raw materials; for the root - the colour of peridermal cells, the degree of development of pith rays, the remainder of the primary xylem; a distinctive feature of the annual root from two or three annuals is the absence of scleroids). It was determined the borderline boundaries of indicators in the series of plant raw materials: loss on drying (not more than 13. 5 %), total ash (not more than 11 %), extractable matter (not less than 33 %) and the quantitative content of amount of hydroxycinnamic acids (not less than 1.3 %) and total polyphenols (not less than 3.5 %). It was identified chlorogenic and neochlorogenic acids. Conclusions. It was determined the type of underground organs of Rumex confertus: annuals had only roots (tap root systems), from the second to the third year of life the plants have both rhizomes and tap root system. It was established their morphological and anatomical diagnostic features and determined numerical indicators and the quantitative content of the amount of hydroxycinnamic acids and total polyphenols with using modern methods of analysis and chlorogenic and neochlorogenic acids were identified. The obtained data will be used in further research, including the development of the draft monograph of the State Pharmacopoeia of Ukraine 2.0 or the draft methods of quality control of medicinal plant raw materials Rumex confertus and the creation of herbal medicines
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来源期刊
ScienceRise: Pharmaceutical Science
ScienceRise: Pharmaceutical Science Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.70
自引率
0.00%
发文量
39
审稿时长
6 weeks
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