Yi Zhang, Chuanhua Fu, Rongrong Zhou, Zi-Yu Liu, Chen Feng
{"title":"Effect of prechilling and exogenous gibberellin on seed germination of Primulina eburnea: a calcium-rich vegetable","authors":"Yi Zhang, Chuanhua Fu, Rongrong Zhou, Zi-Yu Liu, Chen Feng","doi":"10.15258/sst.2023.51.1.01","DOIUrl":"https://doi.org/10.15258/sst.2023.51.1.01","url":null,"abstract":"Calcium-rich food and calcium supplements are expensive, especially for people in developing countries. Primulina eburnea is a calcium-rich vegetable with a high soluble calcium content in its leaves. In this study, we investigated the effects of prechilling and exogenous gibberellin on P. eburnea seed germination. Our results showed that both prechilling time and exogenous gibberellin affect P. eburnea seed germination. Three days prechilling increased germination percentage by 20.7-40.6%; mean germination time and time to first germination were reduced, indicating faster germination. A longer cold treatment enhanced the germination percentage but delayed the germination time. Prechilled P. eburnea seeds germinated 17.3-40.6% more when they were treated with GA3, but high GA3 did not shorten the germination time. Combination of three days prechilling and 50 ppm GA3 is the optimal treatment for P. eburnea seed germination. This research provides a powerful tool to accelerate breeding and cultivating this calcium-rich vegetable.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Seed priming stimulates germination and early seedling establishment of Corethrodendron multijugum under drought stress","authors":"ZhengSheng Li, Y. Liu","doi":"10.15258/sst.2023.51.1.06","DOIUrl":"https://doi.org/10.15258/sst.2023.51.1.06","url":null,"abstract":"Seed priming can alleviate the negative effects of drought stress. The present study focused on exploring the effects of seed priming, drought and their interaction on the germination characteristics of Corethrodendron multijugum. We attempted to standardise an appropriate priming method for C. multijugum seeds by comparing the effects of various priming methods and drought stress on seed germination and seedling establishment to provide theoretical basis and technical support for subsequent cultivation and production. The results indicated that seed priming, drought stress and their interaction significantly (P < 0.01) affected all seed germination parameters and seedling establishment parameters, and germination was significantly (P < 0.05) correlated with early seedling establishment under drought stress. Of the different priming agents used in this study, water, 0.5% KMnO4, 0.3% H2O2 and 5% PEG-6000 significantly (P < 0.05) promoted seed germination and seedling establishment in C. multijugum. Among them, 0.5% KMnO4 was the most effective in alleviating the effects of drought stress on germination and early seedling establishment. Morphologically, the beneficial effects of priming can be attributed to increased seed germination, elongation and biomass accumulation of seedlings.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Chauhan, Satbeer Singh, S. Pandey, Ajay Kumar, M. Kumari, Anil Kumar, A. Thakur, Sanatsujat Singh
{"title":"Alleviation of seed dormancy in kala zeera (Bunium persicum) through chilling and phytohormones","authors":"R. Chauhan, Satbeer Singh, S. Pandey, Ajay Kumar, M. Kumari, Anil Kumar, A. Thakur, Sanatsujat Singh","doi":"10.15258/sst.2023.51.2.03","DOIUrl":"https://doi.org/10.15258/sst.2023.51.2.03","url":null,"abstract":"Kala zeera (Bunium persicum) is a high-value medicinal spice plant. The seeds and extracts are used for culinary, perfumery, flavouring food and medicinal purposes. The commercial cultivation of kala zeera is hampered due to poor seed germination and deep physiological dormancy. To break the dormancy of kala zeera, three phytohormones, gibberellic acid (100 µmol L-1), 6-benzylaminopurine (10 µmol L-1) and thidiazuron (6.3 µmol L-1) were tested individually and in combination, under two temperature levels: continuous chilling at 4°C and one week chilling at 4°C followed by incubation at room temperature. The combined application of the three phytohormones under continuous chilling (4°C) improved the germination from 11.25 to 93.25% and reduced the mean germination time 60 to 50 days. The lowest mean germination time (34.78 days) and high mean daily germination (1.57 seeds day-1) with a high coefficient of germination velocity (2.88), were found under combined application of phytohormones as compared to their individual application. The findings have implications in successful propagation and systematic cultivation of this high-value medicinal spice plant.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of saline treatment on seed germination of adzuki beans","authors":"Shuting Han, Kangyu Lu, Tianbing Guo, Ying Zhang, Binbin Wang, Baomei Wu","doi":"10.15258/sst.2023.51.1.04","DOIUrl":"https://doi.org/10.15258/sst.2023.51.1.04","url":null,"abstract":"Salinity is one of the major causes of abiotic stress for plant development and growth worldwide, and affects crop yield significantly. To analyse the level of salinity tolerance among ‘Ji Hong 20’ (JH20), ‘Jin Xiao Dou 5’ (JXD5) and ‘Jin Xiao Dou 6’ (JXD6) adzuki bean cultivars, nine saline levels (0.0, 32.5, 65.0, 97.5, 130.0, 162.5, 195.0, 227.5 and 260.0 mmol L-1 NaCl) were applied during seed germination. Germination potentials and final germination percentages of all cultivars were significantly reduced by NaCl with the level at which germination was affected being dependent on cultivar. The JXD5 seeds had the highest germination index and vigour index at the same NaCl concentrations, followed by the JH20 and then JXD6 seeds. The shoot and root lengths of JH20 and JXD5 were significantly shorter at NaCl concentrations > 65.0 mmol L-1, while those of JXD6 were reduced at salinity > 32.5 mmol L-1. Quadratic regression models could well predict the germination percentage of JH20, JXD5 and JXD6 seeds under saline stress.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Editorial: Seed Science and Technology. Volume 51 Issue 1 (2023)","authors":"B. S. Olisa","doi":"10.15258/sst.2023.51.1.11","DOIUrl":"https://doi.org/10.15258/sst.2023.51.1.11","url":null,"abstract":"Seed quality is the basis for differential crop productivity. Seed companies depend on the quality of the seeds for the seed trade, while farmers depend on the quality of the seeds to improve their productivity and secure their livelihood. Germination and varietal identification/purity are fundamental aspects of seed quality that are of prime importance to the global seed trade. Seed germination is influenced by several factors among which are the genetic factors and environmental cues of the prevailing growing conditions where the plant grows. Among these cues, climate change fundamentally affects crop development at all stages, starting from germination and seedling growth, to flowering and fruiting. Several methods have been formulated and validated for the evaluation of the varietal identification/purity for some crops, facilitate synchronised seed germination and enhance uniform field establishment for optimum crop yield in the face of climate change. This issue of Seed Science and Technology includes papers discussing the application of some of the methods, for example, prechilling and the use of exogenous gibberellin to stimulate germination, the use of tetrazolium as a quick viability test for rice seeds, the applied phenol test for varietal identification of rice seeds, and seed priming to enhance germination of Corethrodendron multijugum seeds. In addition, the physiological response of seed to soil salinity and storage environment conditions are also discussed.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Seed dormancy and germination ecology of several clover species","authors":"Hamed Rezaei-Manesh, Farshid Ghaderi-Far, Iraj Nosratti, Asieh Siahmarguee, Bhagirath S. Chauhan","doi":"10.15258/sst.2023.51.3.06","DOIUrl":"https://doi.org/10.15258/sst.2023.51.3.06","url":null,"abstract":"This research was carried out to evaluate the effect of (1) different methods of breaking seed dormancy (physical and chemical scarification, high temperature, hot water and after-ripening), and (2) environmental factors (temperature, salinity and water stress) on seed germination of Trifolium angustifolium , T. arvense , T. fragiferum , T. fucatum , T. repens , T. subterraneum and Trifolium sp. All experiments were conducted in the Seed Research Laboratory of Gorgan University of Agricultural Sciences and Natural Resources, Iran, in 2021. All the examined clover species were dormant after harvest, with little germination. Mechanical scarification with sandpaper or sulphuric acid resulted in more than 88 and 52&percnt; germination, respectively. Seeds were able to germinate between 5 and 35&deg;C, but the highest germination (&ge; 98&percnt; on average) occurred at 20&deg;C. Fluctuating temperature regimes were more efficient in improving the germination than constant temperatures. Seeds of clover species, in addition to having a hard seed coat, have a non-deep physiological dormancy. Fresh seeds showed a high sensitivity to water stress compared with after-ripened seeds. However, relative resistance to salinity stress was observed. The results obtained in this research can be used to develop effective sowing strategies and support the successful establishment of these clover species.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135403766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tamiru Olbana, Alice Muchugi, Yeshi Woldemariam, Fiona R. Hay, Nicholas Ndiwa, Chris S. Jones
{"title":"Overcoming dormancy in <i>Desmanthus virgatu</i> seeds for improved viability monitoring protocols of genebank accessions","authors":"Tamiru Olbana, Alice Muchugi, Yeshi Woldemariam, Fiona R. Hay, Nicholas Ndiwa, Chris S. Jones","doi":"10.15258/sst.2023.51.3.12","DOIUrl":"https://doi.org/10.15258/sst.2023.51.3.12","url":null,"abstract":"Desmanthus virgatus is a perennial forage legume used as a livestock feed source. Seeds of this species are physically dormant and thus, propagation is difficult. The dormancy also impacts the routine viability monitoring of genebank accessions. The present study investigated different methods to break dormancy in seeds collected from various locations at different times. The result showed that most pre-treatments significantly increased germination percentage. Accessions treated with concentrated sulphuric acid (25 or 30 minutes) followed by sandpaper scarification showed the highest proportion of normal seedlings (94.6 and 96%). Boiling water (2.3 or 3 minutes) was also effective in all accessions except for accession 331. However, accessions treated with hot water (3, 5 or 8 minutes) had a considerable proportion of hard seeds. The proportion of dead/abnormal seedlings was high in seeds treated with a hot wire in all accessions, ranging from 27.5 (accession 15489) to 75.5% (accession 16). The untreated seeds remained hard at the final germination count. We conclude that it is important to use concentrated sulphuric acid and mechanical scarification with sandpaper to break the dormancy for the species to enhance germination, obtain uniform field stands and make wise decisions during storage.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136367501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dandan Zhao, Hongyuan Ma, Shaoyang Li, Wenwen Qi, Yi Li, Yaxiao Li, Mengdan Sun
{"title":"Divergent germination responses to fire-related cues of Suaeda glauca dimorphic seeds under salinity stress","authors":"Dandan Zhao, Hongyuan Ma, Shaoyang Li, Wenwen Qi, Yi Li, Yaxiao Li, Mengdan Sun","doi":"10.15258/sst.2023.51.3.04","DOIUrl":"https://doi.org/10.15258/sst.2023.51.3.04","url":null,"abstract":"Suaeda glauca is an annual herb in the family Amaranthaceae that produces dimorphic seeds (soft brown seeds and hard black seeds) on the same plant under natural conditions. Experiments were conducted to determine the salinity-alleviating effect of fire-related cues. Germination of both black and brown seeds was inhibited with an increase in salinity. Brown seeds had a higher germination percentage than black seeds in all conditions. Heat shock, smoke and their interaction had some effect in alleviating the salinity effect in brown seeds at 100-300 mM NaCl. This study provides valuable information to the understanding of the germination biology of Suaeda glauca.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67092162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Invited Review - Seed priming: what’s next?","authors":"Irfan Afzal","doi":"10.15258/sst.2023.51.3.10","DOIUrl":"https://doi.org/10.15258/sst.2023.51.3.10","url":null,"abstract":"Seed priming is a pragmatic enhancement technology to improve seed performance used from ancient to modern times. Priming accelerates and synchronises the germination of seeds across a wider range of temperatures, giving rapid and uniform stand establishment of crop plants, particularly for low vigour and dormant seeds. Further investigation is necessary to extend the utilisation of low-cost and effective priming agents to a broader spectrum of species and to enhance availability. Seed priming is a cost-effective solution for small farmers as it reduces production costs and leads to uniform picking and harvesting, thus adding value in terms of quality. It is essential to scale up these approaches at both farmer and industry levels, particularly in developing countries. The benefits of priming are attributed to various putative molecular mechanisms such as epigenetic control of priming, germination, and dormancy-related attributes. However, in different species and cultivars, it remains elusive and there is a paucity of studies on the long-term benefits of re-drying after priming. This in-depth review summarises aspects of priming techniques in a modern context.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135953838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Valentina Vasile, Adina Tîrziu, Elena Nicolae, M. Ciucă, C. Cornea
{"title":"SSR markers are useful tools in wheat varietal purity and genetic diversity assessment","authors":"Valentina Vasile, Adina Tîrziu, Elena Nicolae, M. Ciucă, C. Cornea","doi":"10.15258/sst.2023.51.2.01","DOIUrl":"https://doi.org/10.15258/sst.2023.51.2.01","url":null,"abstract":"Assessing genetic similarity and purity degree among wheat cultivars is important in breeding programmes and SSR markers have all the features that make them suitable for this purpose. The aim of this study was to assess the varietal purity and genetic diversity among wheat cultivars grown in Romania using 22 SSR markers and to choose the most informative markers. The 40 samples included in the study consisted of seeds from certified and non-certified wheat cultivars, and from other related species. To establish that varietal purity is maintained, seeds from 14 certified wheat cultivars were analysed from two harvesting campaigns (years 2019 and 2020) and seeds of four of them were also compared with the authors’ seeds. The most polymorphic SSR marker was Xwmc596 (seven alleles), followed by Xwmc603 and Xwmc474 (six); Xgwm71, Xbarc347 and Xbarc074 (five); Xgwm469, DuPw004, Xgwm155 and Xbarc184 (four); Xwmc418, Xbarc170, Xwmc533, DuPw167, DuPw115, Xgwm413 and Xgwm372 (three); and DuPw217, DuPw205, Xgwm003 and Xgwm095 (two). SSR marker Xgwm052 showed low polymorphism (one). No contamination of the varieties was observed in these harvesting campaigns since all SSRs markers showed high similarity in the certified wheat cultivars harvest analysis; genetic diversity between genotypes was also observed.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67091767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}