Science in China. Series B, Chemistry, life sciences & earth sciences最新文献_第6页

Abrupt reduction of c-myc expression by antisense RNA inducing terminal differentiation and apoptosis of a human esophageal cancer cell line. 通过反义RNA诱导人食管癌细胞系终末分化和凋亡的c-myc表达突然降低。

Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-05-01

X Zhao, X Wang, C Zhou, R Peng, S Yan, M Wu

{"title":"Abrupt reduction of c-myc expression by antisense RNA inducing terminal differentiation and apoptosis of a human esophageal cancer cell line.","authors":"X Zhao, X Wang, C Zhou, R Peng, S Yan, M Wu","doi":"","DOIUrl":"","url":null,"abstract":"A human esophageal cancer cell line (EC8712) expressing high-level Myc protein was infected with recombinant retroviral particles (pA-BD9) at a multiplicity of infection (MOI) 1:1. This viral particle contains a neomycin-resistant gene and a 1.53-kb antisense RNA spanning the 2nd exon and its flanking sequences of the human c-myc oncogene. The G418-resistant EC8712 clones showed an 86% inhibition of growth rate and morphological changes characteristic of terminal differentiation and apoptosis. A decrease of about 80% of Myc protein was also observed in these infected cells by ABC-ELISA assay. 12-24 h after the infection of EC8712 cells with pA-BD9 at a high viral particle concentration (MOI = 1:10), the integration of the extrinsic 1.53-kb antisense c-myc fragment into the cancer cell genome was evidenced by the Southern blot analysis. Northern blot analyses showed the expression of this antisense fragment and a decrease of the intrinsic c-myc expression by 74% in comparison with that of the parental EC8712 cells. Heterotransplants of the infected EC8712 cells into the nude mice revealed a substantial decrease in tumorigenicity and morphological changes characteristic of terminal differentiation and apoptosis. Primary monolayer cell cultures of normal epithelia derived from the fetal and adult esophagus mucosa were set as controls. No noticeable increase in c-myc expression was found in these cultures. Infection of these cells with the same recombinant viral particles neither affected the growth rate of the cells nor their normal morphology. Our experiments indicate that the drastic decrease of the over-expressed Myc protein in cancer cells may also be an entrance to one of many pathways leading to the terminal differentiation and programmed cell death.","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"38 5","pages":"580-9"},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18630251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis and PCR detection of antigen compositions of ovine progressive pneumonia virus. 绵羊进行性肺炎病毒抗原组成分析及PCR检测。

Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-05-01

E Ding

{"title":"Analysis and PCR detection of antigen compositions of ovine progressive pneumonia virus.","authors":"E Ding","doi":"","DOIUrl":"","url":null,"abstract":"Ovine progressive pneumonia virus (OPPV) was proliferated utilizing sheep foetal lung cells, and the cytopathic effect (CPE) of the virus was investigated. OPPV was purified with a 10%-sucrose cushion and then with 20%-55% discontinuous sucrose density gradient centrifugation. The structural proteins and antigen compositions of OPPV were analysed by SDS-PAGE and Western blotting. Besides, the OPP proviral cDNAs of the virus-infected cell cultures and the peripheral blood monocytes from sheep infected by the virus were detected using polymerase chain reaction (PCR). The results show that the CPE of sheep foetal lung cells infected by OPPV is typical of the disease. The purified virions are intact and of high purity when observed with an electron microscope. OPPV proteins consist of 18 polypeptide bands and the molecular weights range from 18 to 120 kd. Among these, 3 were glycoproteins (designated by gp120, gp50 and gp47). The appearance and peak time of the p28 antibody from sheep inoculated with OPPV are earlier than those of the p94 antibody to OPPV. Besides, the strength of immune reaction of p28 antibody is greater than that of p94 antibody. With PCR, it is demonstrated that the initial time for OPP proviral cDNAs to be integrated into the sheep foetal lung cells and the peripheral blood monocytes in sheep were 24 h and 9 d after inoculation, respectively.","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"38 5","pages":"573-9"},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18630250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction between intercalation type anticancer drugs and DNA studied by ultraviolet resonance Raman spectroscopy. 用紫外共振拉曼光谱研究嵌入型抗癌药物与DNA的相互作用。

Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-05-01

X Zhao, D Lu, S Jiang, C Mao, Y Fan, C An, Z Li

引用次数: 0

激光激励的Ca( 1 P 1 )与氯代甲烷系列化合物的反应研究激光激励的Ca( 1 P 1 )与氯代甲烷系列化合物的反应研究

Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-04-10 DOI: 10.1360/ZB1995-25-4-358

丁国文徐大力孙维忠顾月姝何国钟楼南泉杨文胜

引用次数: 0

PDI-, PPI- and chaperone-catalyzed refolding of recombinant human IL-2 and GM-CSF. PDI, PPI和伴侣催化重组人IL-2和GM-CSF的再折叠。

Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-04-10 DOI: 10.1360/YB1995-38-4-429

M. Xu, W. Meng, X. Ma

引用次数: 2

红细胞胞质因子对K 562 细胞分化及其核基质-中间纤维体系影响的研究红细胞胞质因子对K 562 细胞分化及其核基质-中间纤维体系影响的研究

Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-04-10 DOI: 10.1360/ZB1995-25-4-387

薛社普马文丽, Xue She-Pu Ma Wen-Li

引用次数: 0

Stable oncogenic transformation induced by microcell-mediated gene transfer. 微细胞介导的基因转移诱导的稳定的致癌转化。

Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-04-10 DOI: 10.1360/YB1995-38-4-448

Y. Lü, D. Blair

{"title":"Stable oncogenic transformation induced by microcell-mediated gene transfer.","authors":"Y. Lü, D. Blair","doi":"10.1360/YB1995-38-4-448","DOIUrl":"https://doi.org/10.1360/YB1995-38-4-448","url":null,"abstract":"Oncogenes have been identified using DNA-mediated transfection, but the size of the transferable and unrearranged DNA, gene rearrangement and amplification which occur during the transfection process limit the use of the techniques. We have evaluated microcell-mediated gene transfer techniques for the transfer and analysis of dominant oncogenes. MNNG-HOS, a transformed human cell line which contained the met oncogene mapping to human chromosome 7 was infected with retroviruses carrying drug resistance markers and used to optimize microcell preparation and transfer. Stable and drug-resistant hybrids containing single human chromosomes as well as the foci of the transformed cells containing the activated met oncogene and intact human chromosomes were obtained. Hybridization analysis with probes (i.e. col1A2, pJ3.11) mapping up to 1 Mb away from met shows that the cells from the individual foci contain different amounts of apparently unrearranged human DNA associated with the oncogene, and the microcell-generated transformants retain more distal markers than those observed in either DNA- or chromosome-mediated transfers. In conjunction with other techniques, microcell fusion should be useful for gene mapping as well as the study of gene function and expression in cell transformation and malignancy.","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"29 1","pages":"448-56"},"PeriodicalIF":0.0,"publicationDate":"1995-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72646383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mg 2+ 对猪心线粒体H + -ATP酶复合体的激活与质子通道的关系 Mg 2+ 对猪心线粒体H + -ATP酶复合体的激活与质子通道的关系

Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-04-10 DOI: 10.1360/ZB1995-25-4-379

封朝阳邓君鹏林治焕李生广, Bian Chao-Yang Deng Jun-Feng Lin Chi-Huan LI Sheng-An

引用次数: 0

PDI-, PPI- and chaperone-catalyzed refolding of recombinant human IL-2 and GM-CSF. PDI, PPI和伴侣催化重组人IL-2和GM-CSF的再折叠。

Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-04-01

M Xu, W Meng, X Ma

引用次数: 0

Stable oncogenic transformation induced by microcell-mediated gene transfer. 微细胞介导的基因转移诱导的稳定的致癌转化。

Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-04-01

Y Lü, D G Blair

{"title":"Stable oncogenic transformation induced by microcell-mediated gene transfer.","authors":"Y Lü, D G Blair","doi":"","DOIUrl":"","url":null,"abstract":"Oncogenes have been identified using DNA-mediated transfection, but the size of the transferable and unrearranged DNA, gene rearrangement and amplification which occur during the transfection process limit the use of the techniques. We have evaluated microcell-mediated gene transfer techniques for the transfer and analysis of dominant oncogenes. MNNG-HOS, a transformed human cell line which contained the met oncogene mapping to human chromosome 7 was infected with retroviruses carrying drug resistance markers and used to optimize microcell preparation and transfer. Stable and drug-resistant hybrids containing single human chromosomes as well as the foci of the transformed cells containing the activated met oncogene and intact human chromosomes were obtained. Hybridization analysis with probes (i.e. col1A2, pJ3.11) mapping up to 1 Mb away from met shows that the cells from the individual foci contain different amounts of apparently unrearranged human DNA associated with the oncogene, and the microcell-generated transformants retain more distal markers than those observed in either DNA- or chromosome-mediated transfers. In conjunction with other techniques, microcell fusion should be useful for gene mapping as well as the study of gene function and expression in cell transformation and malignancy.","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"38 4","pages":"448-56"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18785970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0