C. Ambrosio, A. C. Miano, E. Saldaña, E. M. Da Gloria
{"title":"A citrus essential oil causes higher disturbance on the growth kinetics of Enterococcus faecalis than Lactobacillus rhamnosus","authors":"C. Ambrosio, A. C. Miano, E. Saldaña, E. M. Da Gloria","doi":"10.17268/sci.agropecu.2022.034","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2022.034","url":null,"abstract":"Essential oils (EOs) have turned a promising alternative to using antibiotics in poultry production due to their antimicrobial properties. EOs could effectively combat pathogenic bacteria affecting poultry. Particularly, Citrus EOs, a by-product of citrus processing industries, could be a feasible alternative to this end due to their vast availability in the global market. Enterococci are associated with intestinal and extra-intestinal infections in poultry, which can increase poultry mortality. On the other hand, Lactobacilli are beneficial bacteria inhabiting the poultry gut and have health-promoting effects. The aim of this study was to evaluate the antibacterial activity of a commercial citrus EO, Orange oil phase essence (OOPE), on Enterococcus faecalis and Lactobacillus rhamnosus as well as to determine OOPE chemical composition. Results showed that OOPE inhibited E. faecalis and L. rhamnosus at 14.8 mg/mL. However, the evaluation of OOPE effects on the growth kinetics parameters of both bacteria reveled that OOPE caused higher disturbances on the growth kinetics of E. faecalis than L. rhamnosus. OOPE significantly reduced the maximal culture density (A) and growth rate (µmax) and extended the lag phase duration (λ) of E. faecalis in a dose-dependent manner, while OOPE slightly extended λ and affected µmax of L. rhamnosus. OOPE at 3.70 mg/mL reduced A and µmax in ~87.34 and 90.2%, respectively, while increased λ 3.8 times of E. faecalis. OOPE at this concentration reduced µmax in 11.8% and extended λ 1.38 times of L. rhamnosus. Therefore, OOPE had a selective antibacterial activity, presenting higher activity on E. faecalis. Despite, limonene was identified as the major compound (87.22%) of OOPE, minor compounds such as trans-carveol could be involved in conferring the selective antibacterial activity of OOPE.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45200942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jennifer Andrea Sierra-Moya, C. Espinoza, O. Guzmán-López, Á. Trigos, Alejandro Salinas Castro
{"title":"Characterization of Fusarium species that affect the asparagus crops cultivation in Veracruz, Mexico","authors":"Jennifer Andrea Sierra-Moya, C. Espinoza, O. Guzmán-López, Á. Trigos, Alejandro Salinas Castro","doi":"10.17268/sci.agropecu.2022.031","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2022.031","url":null,"abstract":"Asparagus cultivation is highly profitable and is appreciated in international gourmet cuisine. In Mexico, the production of this vegetable is mainly for export, in the state of Veracruz it has been recently introduced. The objective of the research was to characterize by morphology, molecular techniques and pathogenicity test the Fusarium species isolated from symptomatic tissues of asparagus shoot. A directed sampling was carried out on plants that showed rot, wilting and dark brown spots. Fungal isolates were obtained from diseased shoots, which were disinfected and seeded on potato dextrose agar. Isolates were purified in monosporic cultures by hyphal tip. Four species of Fusarium were identified by taxonomic morphology, in a selective carnation leaf agar medium, then pathogenicity tests were carried out, complying with Koch's postulates. Next, the strains that were pathogenic were characterized by molecular techniques, and their identity as Fusarium oxysporum and Fusarium proliferatum was confirmed. The information obtained allows the implementation of appropriate management strategies for pathogenic species and thereby avoids economic losses in the short, medium, and long term in the asparagus crop.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46475102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Paucar-Menacho, Rebeca Salvador-Reyes, Williams Esteward Castillo-Martínez, Wilson Daniel Símpalo-López, Anggie Verona-Ruiz, Alicia Lavado-Cruz, Soledad Quezada-Berrú, William López-Rodriguez
{"title":"Use of Andean pseudocereals in beer production","authors":"L. Paucar-Menacho, Rebeca Salvador-Reyes, Williams Esteward Castillo-Martínez, Wilson Daniel Símpalo-López, Anggie Verona-Ruiz, Alicia Lavado-Cruz, Soledad Quezada-Berrú, William López-Rodriguez","doi":"10.17268/sci.agropecu.2022.036","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2022.036","url":null,"abstract":"Beer is one of the most consumed alcoholic beverages worldwide; however, with consumers more aware of food's environmental and nutritional benefits, there is a demand for more sustainable and value-added products. Andean pseudocereals (APS) are becoming a trend as gluten-free grains with excellent nutritional and nutraceutical value. Cultivated under the extreme climate conditions of the Peruvian Andes, APS seeds are an excellent source of starch, fiber, proteins, minerals, vitamins, and bioactive compounds such as phenolic acids and flavonoids with antioxidant capacity that provide potential health benefits. This review is a compilation of the information and studies on the general characteristics and potential of the main APS as quinoa, amaranth, and cañihua for beer production. In addition, current evidence of the malting process in APS and its effects on beer's nutritional, functional, and sensory properties are also provided. Based on the research supporting the pseudocereals inclusion for the formulation of foods for celiac people, this review also looks at recent advances in applying APS to develop traditional and gluten-free (GF) beers. Finally, APS's economic, cultural, and social importance in the brewing industry is highlighted to promote the creation and innovation of fermentative products based on their bioactive compounds, thus achieving better commercial exploration of these ancestral grains.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48635074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hipatia Delgado, C. Tapia, Edwin Borja, Edwin Naranjo, J. P. Martín
{"title":"Phenotypic diversity of Amaranthus quitensis Kunth landraces: A millenary crop of Ecuadorian Andean region","authors":"Hipatia Delgado, C. Tapia, Edwin Borja, Edwin Naranjo, J. P. Martín","doi":"10.17268/sci.agropecu.2022.035","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2022.035","url":null,"abstract":"The black amaranth (Amaranthus quitensis Kunth) is traditionally cultivated in the Ecuadorian highlands, where it has great social, cultural and food importance for the native population, and locally it is called ataco or sangorache. Currently, there are no data on the diversity and conservation status in which the ataco landraces are found. We have evaluated the phenotypic diversity of black amaranth landraces collected at two different times (1981-1986 and 2014-2015) in three representative Andean provinces for this crop (Imbabura, Tungurahua and Cañar). Thirty agro-morphological descriptors were used, 11 quantitative and 19 qualitative. Most of the quantitative traits showed high levels of variation, especially grain yield per plant. The qualitative traits showed less diversity, although the characters related to the plant pigmentation and the shape and attitude of the inflorescence were significantly discriminating between accessions from different provinces. Few significant differences were detected between both collects, so the farmers seem to be carrying out adequate on-farm conservation of the ataco germplasm. The agro-morphological information obtained in this study may be useful for enhancing the use of this plant genetic resource in future amaranth breeding programs, as well as for effectively managing the black amaranth collection from Germplasm Bank of the Ecuadorian National Institute for Agricultural Research (INIAP).","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41859817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meryemnur Kocadağ, P. Şanlıbaba, R. Kasım, M. Kasım
{"title":"Natural and commercial antimicrobial agents that inhibit the growth of Listeria monocytogenes strains","authors":"Meryemnur Kocadağ, P. Şanlıbaba, R. Kasım, M. Kasım","doi":"10.17268/sci.agropecu.2022.032","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2022.032","url":null,"abstract":"Vinegar products have gained popularity as an all-natural antimicrobial agent in recent years. In the present study, the antimicrobial susceptibility of 29 Listeria monocytogenes strains isolated from ready-to-eat foods was detected against natural and commercial antimicrobial agents, vinegar produced from different raw materials, lemon juice, sodium bicarbonate, and hydrogen peroxide, by using the disc diffusion method. Different concentrations of antimicrobial agents were tested against varying cell densities of the L. monocytogenes strain (105, 106, and 107 CFU/mL). The inhibition zone diameter was measured to evaluate the antimicrobial effects of antimicrobial agents on L. monocytogenes strains. The inhibition zone diameters of L. monocytogenes strains were 6.0–8.8 mm and 6.0–11.3 mm against traditional and commercial vinegar types, respectively. The commercial vinegar types showed higher antimicrobial efficacy than the conventional ones. The inhibition zone diameters of L. monocytogenes strains against carbonate, lemon juice, and hydrogen peroxide were 6.0, 6.0–9.0, and 33.9–51.9 mm, respectively. The antimicrobial efficacy of hydrogen peroxide was the highest among the tested antimicrobial agents. This study showed that vinegar products have potential to be utilized as natural antimicrobials on food-borne pathogens like L. monocytogenes.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42534775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Trophic networks associated with the aphid, Rhopalosiphum maidis (Fitch) (Hemiptera: Aphididae) in a cornfield, Manabí, Ecuador","authors":"Jesús Centeno-Parrales, D. Chirinos, T. Kondo","doi":"10.17268/sci.agropecu.2022.029","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2022.029","url":null,"abstract":"The corn leaf aphid is considered an important pest associated with maize. This study aimed to discover the trophic associations around Rhopalosiphum maidis in Manabí, Ecuador. Maize leaves were sampled to determine the numbers of parasitized aphids, and the identities of predators and parasitoids. Nine taxa of natural enemies were detected: the primary parasitoid was Lysiphlebus testaceipes Cresson, 1880 (Hymenoptera: Braconidae); the hyperparasitoid Syrphophagus aphidivorus (Mayr, 1876) (Hymenoptera: Encyrtidae); the predatory hoverfly Ocyptamus dimidiatus (Fabricius, 1781) (Diptera: Syrphidae), four species of coccinellids, Cheilomenes sexmaculata (Fabricius, 1781), Cycloneda sanguinea (Linnaeus, 1763), Hippodamia convergens Guerin-Meneville, 1842 and Paraneda pallidula guticollis (Coleoptera: Coccinellidae) and an assassin bug, Zelus sp. (Hemiptera: Reduviidae). A parasitoid, Pachyneuron formosum Walker, 1833 (Hymenoptera: Pteromalidae) emerged from hoverfly pupae. This study reports the presence of the parasitoids S. aphidivorus and P. formosum in Ecuador for the first time. These results increase the knowledge of a four-trophic level relationship (host plant – pest – parasitoids, predators – hyperparasitoids) in a maize agroecosystem as a fundamental basis for biological control programs.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47489852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nelino Florida Rofner, Mendis Paredes Arce, Ronald Mendis Paredes Salazar, Llerme Navarro Vasquez, Alex Rengifo Rojas
{"title":"An organic management alternative that improves soil quality in cocoa plantations under agroforestry systems","authors":"Nelino Florida Rofner, Mendis Paredes Arce, Ronald Mendis Paredes Salazar, Llerme Navarro Vasquez, Alex Rengifo Rojas","doi":"10.17268/sci.agropecu.2022.030","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2022.030","url":null,"abstract":"The organic management of Theobroma cacao in agroforestry systems is a viable alternative, which is being applied in the Alborada Estate under the name of Mendis Organic Management (MOM) in honor of the researcher and owner of the experimental area. The MOM technology integrates a set of techniques according to the physiological cycle of the plant such as drastic annual pruning, application of compost, foliar biofertilizer, and the use of a variety of cocoa with high planting density. Therefore, the research evaluated the effect of MOM management on soil quality with cocoa plantations (varieties CCN-51 and CMP-15), under agroforestry system developed in the Alborada farm, Leoncio Prado province, Huanuco, Peru. A completely randomized design (CRD) was carried out, the treatments were cocoa areas: CCN-51 of 2x2 m and 10 years of installation (MOM1), CMP-15 of 2x2 m and 4 years (MOM2), CMP-15 of 2x1 m and 5 years (MOM3), CMP-15 of 3x2 m and 13 years (MOM4). Soil physical and chemical indicators were evaluated. All indicators showed significant differences between treatments, the principal component analysis (PCA) considered relevant the sand fraction, clay, OM, N, Ca2+, Mg2+, K+ and CEC, the modeling shows a negative impact in MOM2 and significantly positive in MOM4, regardless of the variety and planting density, observing significant increases for pH, OM, N, P, Ca2+, Ma2+ and CEC. The work shows the great potential of MOM management as a technological alternative to improve soil quality in cocoa plantations under agroforestry systems.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42198849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ruben Torre Alvarez, Diana Nolazco Cama, Lena Tellez Monzón
{"title":"Effect of the conditioning and ultrasound-microwave sequential technique on the yield, chemical and functional properties of the essential oils and hydrolats extracted from the leaves of Tagetes elliptica Smith","authors":"Ruben Torre Alvarez, Diana Nolazco Cama, Lena Tellez Monzón","doi":"10.17268/sci.agropecu.2022.028","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2022.028","url":null,"abstract":"Thermal regulation and ultrasound affect the physical, chemical, and antioxidant properties of essential oils and hydrosols extracted by microwaves from the leaves of Tagetes elliptica S. (chincho). The leaves were conditioned by dehydration or freezing, then pre-treated with ultrasound for 15 or 30 minutes at 40 Hz to finally obtain the essential oil (AECH) and hydrolat (HCH) through microwave extraction (500 W and 30 minutes). Yields, phenolic content, antioxidant capacity, leaf structure by optical microscopy, and chemical composition by gas chromatography and mass spectrometry (GC-MS) were evaluated. The conditioning of the leaves by freezing and sonication for 30 minutes obtained the best performance, 8.86±0.1% and 67.40 ± 4.8%, phenolic content of 39.4±0.1 mg EAG/mL and 346.2 ± 13.6 mg EAG/mL, and antioxidant capacity of 35.7 μmol EqT/mg and 0.25 μmol EqT/mg equivalent, for AECH and HCH, respectively in every case. Structural damage of the leaves was observed due to the effect of the conditioning-ultrasound-microwave treatments, achieving a more significant release of bioactive compounds. The main compounds of the essential oil were tagetenones (49.2%), followed by cis-miroxide (14.5%), dihydrotagetone (13.9%), tagetone (9.8%) and ocimene (2.4%); the main compounds in the hydrolate were cis-myroxide (44.66%), followed by cis- tagetone (23.84%), trans-tagetone (12.24%), linalool oxide (5.48%) and dihydrotagetone (4.04%). The significant contribution of the present study is the extraction techniques and chemical profile of tagetes elliptica extracts.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48834544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raudel Pérez del Rio, Martín Hidalgo Reyes, Magdaleno Caballero Caballero, L. H. Hernández Gómez
{"title":"Multi-objective optimization through artificial intelligence for designing of an Agave angustifolia leaf shredder","authors":"Raudel Pérez del Rio, Martín Hidalgo Reyes, Magdaleno Caballero Caballero, L. H. Hernández Gómez","doi":"10.17268/sci.agropecu.2022.026","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2022.026","url":null,"abstract":"A neural network and a genetic algorithm were used in a hybrid method to get the optimal design parameters of an Agave angustifolia Haw. green leaf shredder. First, a prototype of an experimental machine was built using the design parameters recommended by the literature and calculated using linear equations. Then, the shredder prototype was subjected to experiments. The defibration data with different blade adjustments were obtained with experimental values. The data was configured and trained with an artificial neural network to establish a correlation between the defibration quality and the design parameters. The multi-objective optimization method based on genetic algorithms determined the optimal design parameters of the shredder’s functional mechanical elements. The best point was obtained from the least number of broken fibers (2.83%) and the most waste (73.15%). The method used proved suitable to optimize the design parameters; this was based on actual data obtained by experiments performed with the prototype and then modeled through artificial intelligence methods such as neural networks to determine an optimal solution using evolutionary genetic algorithm methods.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46449852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andrés C. Estrada Zúñiga, Jim Cárdenas, Juan Víctor Bejar, J. Ñaupari
{"title":"Biomass estimation of a high Andean plant community with multispectral images acquired using UAV remote sensing and Multiple Linear Regression, Support Vector Machine and Random Forests models","authors":"Andrés C. Estrada Zúñiga, Jim Cárdenas, Juan Víctor Bejar, J. Ñaupari","doi":"10.17268/sci.agropecu.2022.027","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2022.027","url":null,"abstract":"Remote sensing with large-scale satellite images for precision studies in grasslands has spatial and spectral resolution limitations. Against this, using spectral signs and vegetation indices obtained with microsensors transported by unmanned aerial vehicles (UAV) constitutes a more accurate alternative for biomass estimation. In the fieldwork, images were acquired with microsensors, and fixed transects of 100 m were used where vegetation samples were collected. The photographs acquired with the UAV were processed in Pix 4D, Arc Gis, and algorithms elaborated in R programming language. The biomass estimation was carried out with Multiple Linear Regression, Vector Support Machine, and Random (Forest Random) models. The Random model showed a Kappa coefficient of 0.94 in the training set and 0.901 in the test set (R2= 0.482). The Random Forest model predicted 3 g/pixel of MV for Puna grass in the rainy season and 2 g/pixel for the dry season; the predicted biomass for the Tola bush was 15 g/pixel of MV for bothseasons of the year. The estimation of biomass/hectare for the tolar plant community with its tola shrub and Puna grass components was 6,535.88 kg/ha for the rainy season and 6,588.81 kg/ha for the dry season. The difference between the biomass estimated in the field and the biomass estimated with Random Forestwas 5.48% for the rainy season and 9.63% for the dry season.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43768076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}