ScienceRise: Biological Science最新文献

{"title":"The dependence of the synthesis of melanin by black yeast Psedonadsoniella brunnea on the number of coal sources in the culture medium","authors":"T. Kondratiuk, T. Beregova, T. Akulenko, V. Vereschaka","doi":"10.15587/2519-8025.2019.179939","DOIUrl":"https://doi.org/10.15587/2519-8025.2019.179939","url":null,"abstract":"The aim of the study was to determine the effect of carbon source content in the culture medium on melanin synthesis by black yeast Pseudonadsoniella brunnea 470 FCKU. Materials and Methods: Cultivation of Pseudonadsoniella brunnea strain was carried out on liquid nutrient medium, the main component of which was barley-malt extract. The concentration of the carbon source (carbohydrates) in the barley-malt extract solution was set at 2.0; 4.0; 6.0 and 8.0 % for the AST-2 areometer-sugar meter. 0.05 % L-tyrosine and 1 % peptone enzymatic were added to these media. 0.05 % L-tyrosine and 1 % peptone enzymatic were added to these media. The acidity of the media at the level of 1-1.5 was carried out using sulfuric acid. Result. Studies have shown that the amount of synthesized melanin by a strain of black yeast fungi Pseudonadsoniella brunnea 470 FCKU depends on the amount of carbon source in the culture medium. The amount of melanin synthesized by the Pseudonadsoniella brunnea strain increased by 5.8 and 5.1 times, with a decrease in the content of reducing sugars in the culture medium by 2 and 4 times, respectively. Conclusions: Cultivation of Pseudonadsoniella brunnea 470 FCKU strain at low pH and carbon source content results in its protective function in the form of melanin release into the culture medium. The highest amount of melanin (394.03 mg/l) by strain Pseudonadsoniella brunnea was synthesized when carbon source was introduced into the culture medium at a concentration of 4.0 % by hydrometer, which corresponds to the content of 2.4-2.52 % reducing sugars (predominantly maltose)","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77797013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodiversity of the steps of the Lysa Hora - an element of the regional ecological network of the Odessa region","authors":"Yevheniia Tkach, V. Shavrina","doi":"10.15587/2519-8025.2019.180487","DOIUrl":"https://doi.org/10.15587/2519-8025.2019.180487","url":null,"abstract":"","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"403 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76921248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial distribution, dimensional-mass and age structure of dab of the gloss Platichthys luscus population of Shabolatsk Liman","authors":"M. Burhaz","doi":"10.15587/2519-8025.2019.169657","DOIUrl":"https://doi.org/10.15587/2519-8025.2019.169657","url":null,"abstract":"","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"99 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72839229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and antibioticresistance of lactic acid bacteria isolated from the gastrointestinal tract of a rabbit","authors":"Y. Pohilko, N. Kravchenko","doi":"10.15587/2519-8025.2019.169077","DOIUrl":"https://doi.org/10.15587/2519-8025.2019.169077","url":null,"abstract":"","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91039591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymorphism of gene Hemagglutinin and its influence on the properties of influenza virus a H1N1 and H7N9 strains","authors":"S. Buriachenko, B. Stegniy","doi":"10.15587/2519-8025.2019.168500","DOIUrl":"https://doi.org/10.15587/2519-8025.2019.168500","url":null,"abstract":"","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81507336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Level of Cyclin D1 protein in peripheral blood lymphocytes of Chornobyl clean-up workers in remote period after radiation exposure","authors":"L. Zvarych, N. Golyarnik, I. Ilienko","doi":"10.15587/2519-8025.2019.165703","DOIUrl":"https://doi.org/10.15587/2519-8025.2019.165703","url":null,"abstract":"Aim: to assess changes of products of Cyclin D1 protein in lymphocytes of peripheral blood of Chernobyl clean-up workers as remote results of the action of ionizing irradiation. Methods: there were examined 120 Chernobyl clean-up workers in the remote period after radiation exposure and 45 persons of the control group. For assessing mitogen-induced levels of Cyclin D1, the micro-method of cultivating erythrocytes of whole blood was used. The quantitative assessment of the spontaneous and mitogen-induced levels of Cyclin D1 in lymphocytes of peripheral blood (PB) was realized using the reagents FITC Mouse Anti-Human Cyclin D1 Antibody Set (BD, USA) by the method of flow cytometry.Research results: there was determined the dose-dependent increase of the spontaneous level of Cyclin D1 in PB lymphocytes of Chornobyl clean-up workers. High values of the parameter were established in the subgroup of Chornobyl clean-up workers, irradiated in the diapason of doses as 500-1000 mSv. Maximal values of Cyclin D1 level in PB lymphocytes were observed in Chornobyl clean-up workers at exacerbation of the bronchial-pulmonary pathology, bronchial asthma in anamnesis and in reconvalescents of acute radiation sickness with the radiation doses D ≥ 500 mSv. After mitogen stimulation of lymphocytes, there was noted the decrease of Cyclin D1 level in the group of Chornobyl clean-up workers and the increase in persons of the control group.Conclusions: the research revealed the differences in products of Cyclin D1 in PB lymphocytes of Chornobyl clean-up workers and persons of the control group. The revealed changes of the spontaneous and mitogen-induced levels of Cyclin D1 in PB lymphocytes of Chornobyl clean-up workers with the somatic pathology reflect disorders in regulation processes of proliferation and cellular cycle. The obtained data add ideas about mechanisms of the radiation-induced disorder of the cellular cycle that may be a manifestation of genome instability and become a trigger factor of carcinogenesis in the remote period after radiation exposure","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"10 14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89054403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal dynamics of flowering of Calluna vulgaris (L.) Hull and its cultivars in conditions of Kyiv regarding to peculiarities of the flower structure","authors":"M. Kuznetsova","doi":"10.15587/2519-8025.2019.165608","DOIUrl":"https://doi.org/10.15587/2519-8025.2019.165608","url":null,"abstract":"The aim of our study was to research the connection between the flower structure and the duration of flowering of Calluna vulgaris (L.) Hull and its cultivars. Our tasks were to investigate the flower structure of bud bloomers and double-flowered Calluna vulgaris (L.) Hull cultivars, to compare it with the structure of C. vulgaris flower; to analyze the data of phenological observations of C. vulgaris and its 10 cultivars flowering phase during 2015–2018. The methods were phenological observations and microscopic examinations. Results. Common features for C. vulgaris and cultivars concerning to their flowering are racemose inflorescences, an arrangement of flowers, acropetal succession. The flower structure of ‘Dirry’, ‘Mullion’, ‘Golden Carpet’, ‘Winter Red’ is similar to that of C. Vulgaris. Among this group, ‘Winter Red’ finishes blooming the first and ‘Dirry’ is the last due to its compound inflorescence. An increased number of petals, full sterility and long flowering period characterize flowers of double-flowered cultivars. Bud bloomers ‘Alicia’, ‘Bonita’ belong to f. diplocalyx Jansen. They are characterized by different flowering periods. ‘Alicia’ blooms the longest (85±7 days) among investigated C. vulgaris cultivars and ‘Bonita’ blooms about 53 days. Conclusions. C. vulgaris cultivars that have the same flower structure as species begin to bloom, as well as common heather, in the third decade of July. The duration of C. vulgaris cultivars flowering period depends also on the peculiarities of the inflorescence structure. Therefore, in order to predict the duration of cultivars flowering, it is necessary to study the features of their inflorescences structure. Double-flowered cultivars are worth to be used more often in gardening due to their high decorativeness and long flowering period. The issue of preserving the color of bud bloomers flowers needs further research. When using the cultivars of C. vulgaris with different types of flowers and duration of flowering, we can create landscapes of high and long decorative features","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88224364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of gibberellin on the mesostructural organization of the leaf, accumulation and redistribution of assimilates and feeding elements of the gooseberry plants (Grossularia reclinat) in connection with the productivity of culture","authors":"H. Shataliuk, V. Kuryata","doi":"10.15587/2519-8025.2019.158224","DOIUrl":"https://doi.org/10.15587/2519-8025.2019.158224","url":null,"abstract":"The effect of gibberellic acid on morphogenesis, mesostructural leaf organization, accumulation and redistribution of various forms of carbohydrates, nitrogen, phosphorus and potassium between the organs of gooseberry plants of the Mashenka variety in relation to the productivity of the culture has been established. The aim of the study was to find out the peculiarities of morphogenesis, the formation of the leaf apparatus, the accumulation and redistribution of assimilates and nitrogen-containing compounds between the organs of gooseberry plants for the actions of gibberellic acid in connection with the productivity of the culture. Materials and methods. Plants were treated once during the budding period with a 0.05 % m gibireleic acid solution during the budding phase. The carbohydrate content was determined by the iodometric method, nitrogen - by Keldahl, phosphorus - by the intensity of the formation of the phosphorus-molifbdenovogo complex, potassium - by flame-photometric, the content of chlorophylls - spectrophotometrically. Results . Under the influence of the drug, a more powerful donor sphere was formed due to the growth of chlorenchyma due to an increase in the volume and linear dimensions of the cells of the columnar and spongy assimilation tissue. This led to enhanced assimilation, an increase in the content of non-structural carbohydrates (sugar + starch) in the shoots. Processing gibberellin contributed to the optimization of nitrogen, phosphorus and potassium in the leaves and shoots. A significant deponuvalous capacity of the stems in the temporary redundancy of an aksimilit and gooseberry plant nutrients with the subsequent reutilization of them to the processes of carpogenesis has been established. Changes in the nature of donor-acceptor relations in gooseberry plants under the influence of gibberellic acid caused the redistribution of assimilate and nutrient fluxes from vegetative organs to fruits, and had a positive effect on the crop productivity. Findings. The use of gibberellic acid led to the formation of a more powerful donor sphere, the accumulation and temporary deposit of the part of carbohydrates and mineral nutrients in the vegetative organs of gooseberry plants, followed by their remobilization on the need for fruit formation and growth, led to a significant increase in yield and improved the product quality","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75275671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural-functional state of kidneys of rats of two generations when using glyphosate-resistant genetically modified soy and herbicide Roundup","authors":"I. Chorna","doi":"10.15587/2519-8025.2019.160170","DOIUrl":"https://doi.org/10.15587/2519-8025.2019.160170","url":null,"abstract":"Due to the widespread use of transgenic soy and the Roundup herbicide, we conducted histological studies of the kidneys of two generations of rats that received genetically modified soybean \"Roundup Ready\", not treated with a herbicide, the same genetically modified soybean, treated with a herbicide and the \"Roundup\" herbicide together with drinking water. Materials and methods. The subjects of the study were male rats of the Wistar line at the age of 4 months, weighing 180-200 g. The experimental animals were grouped into 5 groups of 12 rats each (6 females and 6 males): Group I - the intact animals (kept on a standard vivarium diet); Group II – the animals with up to 26% of standard diet nutritional value, replaced by traditional soybean; Group III - the rats with up to 26% of the standard diet, replaced by genetically modified soybeans, not treated with the herbicide Roundup; Group ІV – the rats,  fed with feed containing up to 26% of genetically modified soybean, treated with the herbicide Roundup; Group V – the rats, received the herbicide Roundup with drinking water at a concentration (0.1 μg / L). After 42 days, all groups of rats were paired, and thus the second generation was obtained. At the age of 12 months, the rats of all groups were decapitated, and pieces, selected from the middle part of the kidneys (indicate which ones), then these pieces were fixed in 10 % neutral solution of formalin for 48 hours. At performing morphological studies, commonly used histological techniques were used. Results. The evaluation of histopathological changes in the kidneys of the experimental animals, fed with genetically modified soybean, not treated with the Roundup herbicide (Group III), the ones, fed with transgenic soybean, treated with a herbicide (Group IV), and the ones received the Roundup herbicide with water (Group V) was realized. The analysis of histological sections of the kidneys in all rat groups showed changes in the structure of the kidneys in the IV and V groups. As a result of the experiment, reversible swelling of the renal tubule epithelium in these groups and signs of necrosis of individual cells in the first generation of rats were noted, and in the second generation there was a destruction of the tubule cells and a violation of their functions. Conclusions. The use of genetically modified soybean, treated with the herbicide «Roundup» and herbicide, results in the loss of the structure of renal glomeruli and in the disruption of their functions, both in the first and second generation. Such results indicate a negative effect of the herbicide on the rat's organism and its ability to accumulate in soybean seeds","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78865554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sozophytes and habitat in the territory of the stow Khortytsia under protection of the bern convention","authors":"S. Okhrimenko, Yevheniia Tkach","doi":"10.15587/2519-8025.2019.159799","DOIUrl":"https://doi.org/10.15587/2519-8025.2019.159799","url":null,"abstract":"Aim. In order to preserve the phytocenosis fund of the Khortytsia tract, we set the goal to include it in the territory of a special nature conservation value which is the Emerald Object. To do this, a phytososological assessment of the territory was carried out regarding its compliance with the requirements of the Berne Convention. Results. The Khortitsa stow is the territory which landscapes represent a characteristic of the Zaporizhzhia oblast, especially steppe, with natural vegetation in a well-preserved state. The study of the flora and vegetation was carried out by the expeditionary route method, as well as setting stationary experimental areas of 100 m2 and transecting in accordance with generally accepted methods. On the territory of the tract there were discovered: – All arid types of E1 category herb groups classified EUNIS, described for Zaporizhzhia oblast (Е1.11, Е1.2.1.1 – Е1.2.1.7); – 20 types of habitats, protected by resolution No. 4 of the Bern Convention (C 1.226; C1.33; C1.3411; C 2.33; C2.34; C 3.4; C 3.51; D 5.2; E 1.1.1; E 1.2; E 3.4; E 5.4; G1.11; G 1.3; G 1.7; X 18). – 3 types of vascular plants (Aldrovanda vesiculosa L., Jurinea cyanoides DC., Serratula lycopifolia Beck.), listed in Appendix No. 1 of Resolution No. 6 of the Bern Convention. The obtained data were included into the standard data forms of the objects of the Emerald Network applicants and were defended at the Emerald Network Biogeographical Seminar for all habitats and species (except birds) for the Steppic region (the Republic of Moldova, the Russian Federation and Ukraine), the Alpine Caucasus (the Russian Federation), the Marine Black Sea (Ukraine, the Russian Federation) and the Marine Caspian Sea (which was held in Kyiv from on September 6 till 8, 2016). Conclusion. In 2016 the stow was included in the Emerald Network of Ukraine UA0000106 \"Kakhovka Reservoir\". The research materials show that Khortytsia stow has a great phytososological value and needs arrangements for protection and conservation of its biodiversity","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89194414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}