The dependence of the synthesis of melanin by black yeast Psedonadsoniella brunnea on the number of coal sources in the culture medium

Abstract

The aim of the study was to determine the effect of carbon source content in the culture medium on melanin synthesis by black yeast Pseudonadsoniella brunnea 470 FCKU. Materials and Methods: Cultivation of Pseudonadsoniella brunnea strain was carried out on liquid nutrient medium, the main component of which was barley-malt extract. The concentration of the carbon source (carbohydrates) in the barley-malt extract solution was set at 2.0; 4.0; 6.0 and 8.0 % for the AST-2 areometer-sugar meter. 0.05 % L-tyrosine and 1 % peptone enzymatic were added to these media. 0.05 % L-tyrosine and 1 % peptone enzymatic were added to these media. The acidity of the media at the level of 1-1.5 was carried out using sulfuric acid. Result. Studies have shown that the amount of synthesized melanin by a strain of black yeast fungi Pseudonadsoniella brunnea 470 FCKU depends on the amount of carbon source in the culture medium. The amount of melanin synthesized by the Pseudonadsoniella brunnea strain increased by 5.8 and 5.1 times, with a decrease in the content of reducing sugars in the culture medium by 2 and 4 times, respectively. Conclusions: Cultivation of Pseudonadsoniella brunnea 470 FCKU strain at low pH and carbon source content results in its protective function in the form of melanin release into the culture medium. The highest amount of melanin (394.03 mg/l) by strain Pseudonadsoniella brunnea was synthesized when carbon source was introduced into the culture medium at a concentration of 4.0 % by hydrometer, which corresponds to the content of 2.4-2.52 % reducing sugars (predominantly maltose)