ScienceRise: Biological Science最新文献

{"title":"Characteristics of visible autumn migrations of the Dunnock prunella modularis (Linnaeus, 1758) in National Park “Prypiat-Stokhid” in 2012-2017","authors":"M. Khymyn","doi":"10.15587/2519-8025.2020.223671","DOIUrl":"https://doi.org/10.15587/2519-8025.2020.223671","url":null,"abstract":"The aim. The main purpose of the study was to find out the peculiarities of autumn migrations of birds, in particular such little-studied species as the Dunnock. This is necessary for the development and implementation of a management plan in the National Park for a whole complexes of migratory birds and separate species, first of all rare or small in number quantity.Materials and methods. Our research was conducted at the Prypiat River near Svalovychi village in Liubeshiv district, Volyn region of Ukraine in September and October 2012–2017. Observations were performed visually, starting in the morning half an hour before sunrise and ending in the evening half an hour after sunset (all hours of the light part of a day, without interruption). The light part of a day was divided into hour of observations, taking into the daily changes of sunrise and sunset. Flight altitude was determined visually, and flight directions – by 8 rhumbs.Results. There were 2124 individuals of the Dunnock counted during the study of visible autumn migration birds at one observation point in 2012–2017. The most bird migrated in flocks, but a significant part of them flew alone. The most numerous migrants this species were in small flocks – 2–5 individuals (89.9 % of all counted birds in flocks). There are from 2 to 38 individuals in one flock, on average – 3.2±0.15 individuals. The main passage lasted from the 2nd decade of September to the middle of the 2nd decade of October. The majority of them flew in the morning, in the first 3 hours of observations (75.5–83.5 % of all counted birds in different years, in average 79.6 %). The majority birds of this species were observed in flight within altitudes between 30 and 40 m (59.4 %) and the predominant direction of flight was W (52.4 %) and some less – SW (45.4 %).Conclusions. According to the results of 6-years research, the peculiarities of visible autumn migrations of the Dunnock have been clarified (the main passage – the 2nd decade of September – middle of the 2nd decade of October, majority of birds flew in the first 3 hours of observations, used altitudes between 30 and 40 m and the W and SW directions of passage). The results of our research are the basis for the further study of this species, planning and implementation of conservation measures for birds in the National Park “Prypiat-Stokhid”. The obtained data are also a supplement to the state of study of this species in Ukraine","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"229 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80996047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends and outcomes of tuberculosis among cases at directly observed short course treatment (DOTS) at the Tepi public Health Center Southwest Ethiopia","authors":"S. Getachew, A. Sirna, Abiyot Negash, Abyot Asres","doi":"10.15587/2519-8025.2020.213488","DOIUrl":"https://doi.org/10.15587/2519-8025.2020.213488","url":null,"abstract":"Background: Monitoring treatment outcomes and understanding the reasons for unsuccessful treatment have paramount importance for the tuberculosis control program. This study was designed to evaluate trends and treatment outcomes of tuberculosis patients at the Tepi Health Center and to identify the predictors of unsuccessful treatment outcome.Method: Retrospective review of TB cases, registered in the Tepi health center from 2011-2018, was conducted using data, extracted from medical records of TB patients. The structured data extraction form was prepared and used to extract socio-demographic, clinical and outcome data of study cases. The case definition and the treatment outcome of patients were ascertained and reported in accordance with the World Health Organization guideline. A binary logistic regression model was fit to identify predictors of unsuccessful outcome.Results: A total of 1651 TB patients, registered at the Tepi Public Health Center in between June 2011 and May 2018, were included in the study. Of all 924(56 %) were males and 1053 (63.8 %) cases were in between the age range of 15 and 35 years. HIV-status of 1019 TB cases was unknown and 189(11.4 %) of participants were HIV-positive. 457 (27.7 %) cases were diagnosed with extra pulmonary TB (EPTB) and 1194(72.3 %) were pulmonary TB patients, out of which 376(73.6 %) were smear-positive pulmonary TB (PTB+). The overall treatment success rate (TSR) of patients was 80.4 % (1327/1651), while it was 84.8 % (134/158), 80.2 % (410/511), and 78.3 % (148/189) among the transfer-in, PTB+, and HIV+ cases, respectively. Higher numbers of successful treatment outcomes were recorded among new (82.7 %) EPTB cases (84.7 %). The cure rate was 73.6 %(376/511) and 18(34/189) among patients with PTB+ and HIV+, respectively. Multiple logistic regression analysis indicated that residence sites (OR .763(.584, .996) and TB/HIV co-infection (OR 0.661(0.444, 0.985), were significantly associated with the treatment outcome. Rural residence was 27.1 % less likely to have successful treatment. There was significant heterogeneity in the odds of having successful treatment outcomes across years of initiating treatment.Conclusion: The treatment success rate among study cases was lower than the WHO’s target and further efforts like availability of TB clinics in nearby sites and reducing rate of HIV infection should be made to improve the rate of successful treatment outcome","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82600425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of blood`s microcirculation at physical loads","authors":"T. Stanishevska, Оksana Gorna, D. Horban, O. Yusupova","doi":"10.15587/2519-8025.2020.217693","DOIUrl":"https://doi.org/10.15587/2519-8025.2020.217693","url":null,"abstract":"This research deals with the study of blood microcirculation peculiarities.Materials and methods. 72 students of Bogdan Khmelnytsky Melitopol State Pedagogical University, aged 18–19, were examined. The experimental research consisted of the study of blood microcirculation functional state by means of Laser Doppler flowmetry (LDF) method. It helped to evaluate the state of tissue blood-circulation and to detect individual-typological peculiarities of blood microcirculation under the influence of physical activity (before and after exercise).Results. Three types of blood microcirculation were identified by using LDF-metry. The normoemic type of blood microcirculation, characterized by the superposition of oscillatory rhythms and reflected the balance of the mechanisms of regulation of microcirculation. The hyperemic type, characterized by a «monotonous» LDF-gram with a high parameter of microcirculation, which reflects the relative predominance of metabolic mechanisms in the regulation of microcirculation. The hypoemic type, characterized by a «monotonous» LDF-gram with a low parameter of the microcirculation parameter, which reflects the decrease of vasomotor mechanisms in the regulation of microcirculation. According to the LDF-metric data, the examined students under intensive physical activity have a significant increase in microcirculatory status: by 6 % of the microcirculation parameter, by 28 % of the mean square deviation and by 45 % of the initial value of the coefficient of variation.Conclusions. This dynamics of microcirculation shows that under the influence of physical exertion, a person creates significant functional reserves for the redistribution of blood flow and for more perfect intraorgan capillary blood flow. It was found, that in the process of physical activity, morpho-functional rearrangements of the human cardiovascular system occur. This reaction is formed by several components of blood microcirculation: blood flow in the transport direction, regulating blood supply in accordance with the needs of tissues and the exchange component of the histochemical barrier","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72742746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Еuropean mistletoe (víscum álbum L.) in national botanical garden M.M. Grushko NAS of Ukraine: an overview of its distribution and hosts","authors":"E. Yelpitiforov, Yu. O. Klymenko","doi":"10.15587/2519-8025.2020.213202","DOIUrl":"https://doi.org/10.15587/2519-8025.2020.213202","url":null,"abstract":"The aim. To give a brief literature review of the biological features of Viscum album L., transpiration of these plants, features of the systematic situation, distribution in the world. There was determined the distribution and general trends of the semi-parasite plant on the territory of the National Botanical Garden named after M. M. Grishka NAS of Ukraine. Materials and methods. The research was conducted on the territory of the National Botanical Garden named after M. M. Grishka NAS of Ukraine. Visual inspection of trees and bushes for Viscum album L. was carried out, their location was determined, followed by presentation of the results on the map of the botanical garden. The degree of damage to host plants was determined. Results. The map of the National Botanical Garden (NBG) named after M. M. Grishka of the National Academy of Sciences of Ukraine with the affected plants marked on it, is presented. A generalized list of host plants inhabited by Viscum album L. on the territory of the NBS has been compiled. The most affected plants were identified and found to belong to 7 orders of magnitude. Conclusions. The total number (50) of plants introduced to the NBG, on which Viscum album settles, as well as the areas with the largest number of affected plants were determined. For the results of the study, we examined the V. album, which is supported to the following files Fabales, Fagales, Lamiales, Malpighiales, Malvales, Rosales and Sapindales. Identified a larger number of affected semi-parasites of cultivation - Robinia pseudoacacia, which has many plants in the NBG.","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82669054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecological features of groups of rove beetles (Coleoptera: Staphylinidae) in beech forest ecosystems of the lower forest belt of the Gorgan massif","authors":"Marіana Lutskа, A. Sirenko","doi":"10.15587/2519-8025.2020.214189","DOIUrl":"https://doi.org/10.15587/2519-8025.2020.214189","url":null,"abstract":"Purpose: to study the species composition of groups of predatory beetles in beech forests of the lower forest belt of the Gorgan massif and their ecological features. Materials and methods. Representatives of 54 species from 12 subfamilies. Identification of the reveal species was performed using Bay-Bienko (1965), Bоhас J (1985 a, b), Coiffait, H., (1974, 1984), Lohse, G. A., (1964, 1974) determinants. Determination of dominance groups by the Stecker-Bergman method (1977), establishment of ecological and morphological groups according to Kashcheev V. A. (1982, 1985, 1999), types of life strategies according to Planck's method, Belonging to zoogeographical groups was established by the Vtorov P. P., Drozdov N. N. (2001). Research results. According to the results of the research, 556 individuals of rove beetles belonging to 28 genera were found, which are located within 12 subfamilies. The highest level of species diversity is characterized by the subfamilies Staphylininae and Tachyporinae, which are represented by 21 and 13 species, respectively. Only one dominant species was found in the structure of dominance - Tasgius (Rayacheila) bicharicus Mull., 1825, however, a high number of subrecent species is observed. The identified species are representatives of eleven ecological and morphological groups. Analysis of the trophic specialization of predatory beetles has shown a clear dominance of predators, among which there are both specialized species and polyphagous. The analysis of life strategies revealed representatives of 7 groups, and zoogeographical features - 10. Conclusions. Among the species of predatory beetles caught, there is a clear dominance of tachyporin and staphylin, which total 62.9 % The study of the dominance structure of this group showed a small proportion of mass species, and a clear redominance of recedents and subrecedents. According to the analysis of ecomorphs, there is an increase in the number of wells and cryptobionts, as well as mixotrophs, due to the significant level of ecological niches that are inherent in the analyzed type of ecosystems","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80234060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncarina roeosliana Rauh ontomorphogenesis in early stages of development","authors":"M. Gaidarzhy, O. Futorna, N. Nuzhyna","doi":"10.15587/2519-8025.2020.201320","DOIUrl":"https://doi.org/10.15587/2519-8025.2020.201320","url":null,"abstract":"Мета. З’ясувати морфологію насіння, виявити особливості його проростання та дослідити розвиток рослин Uncarina roeoesliana Rauh (Pedaliaceae) на ранніх стадіях онтоморфогенезу в зв’язку з проблемою схожості насіння та вирощування їх в умовах інтродукції. Матеріали та методи. Досліджено латентний та прегенеративний періоди онтоморфогенезу рослин Uncarina roeoesliana за умов вирощування у захищеному ґрунті Ботанічного саду ім. акад. О. В. Фоміна. В роботі використані біоморфологічні, інтродукційні, гістологічні методи. Результати та їх обговорення. Розроблено метод прискорення схожості свіжозібраного насіння. Встановлено, що насінини Uncarina roeoesliana великі за розмірами (близько 7х5 мм), переважно широкотрикутні, коричневого кольору з малопомітним крилом по всьому периметру насінини. Дорзальна сторона формує складки, рубчик знаходиться на мікропілярній частині насінини. Периклінарні стінки опуклі і часто формують папіли. Зародок великий, займає більшу частину насінини, ендосперм багатий на ліпіди, що характерно і для представника цієї ж родини Sesamum indicum L. Ймовірно наявність папіл сприяє накопиченню вологи для активізаціії ферментативної діяльності при набуханні насінин. Всі ці особливості проростання насіння узгоджуються з екологічними умовами в місцях природного розповсюдження представників роду Uncarina: високими температурами, невеликою кількістю опадів та значним посушливим періодом. Ймовірно рослини цього виду відносяться до макробіотиків, тобто достатньо довго можуть зберігати схожість насіння. Проростання надземне. Впродовж 24 тижнів розвитку рослини досягають віргінільної стадії розвитку: формують редькоподібні корені, 5-6 пар листків, з яких на молодих рослинах у цій стадії залишається тільки дві пари, та потовщену базальну частину стебла. Висновки. Порівнюючи Uncarina roeoesliana з іншими каудексоподібними рослинами можна констатувати, що ця рослина має високий потенціал для виживання в посушливих умовах завдяки здатності рослини накопичувати вологу в базальній частині стебла і м’ясистому корінню. При цьому рослина пристосована до існування як в режимі спокою, так і в режимі активного росту, але в останньому випадку тільки при наявності достатньої кількості вологи в ґрунті Ключові слова: насінина, проростання, ранні етапи онтогенезу, каудексоподібна рослина, ендемік Мадагаскару","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"146 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79280261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quorum sensing autoinducers biosynthesis by biofilm cultures of Pseudomonas aeruginosa strains with different levels of the cyclic diguanozinmonophosphate","authors":"M. Galkin, A. Semenets, B. Galkin, T. Filipova","doi":"10.15587/2519-8025.2020.205217","DOIUrl":"https://doi.org/10.15587/2519-8025.2020.205217","url":null,"abstract":"Мета роботи – встановити залежність між вмістом цикло-ди-ГМФ і здатністю P. aeruginosa до утворення біоплівки і синтезу аутоіндукторів системи кворуму. Матеріали та методи дослідження. В роботі були використані штам дикого типу P. aeruginosa PA01 і штами P. aeruginosa з низьким (PAO1pJN2133) та підвищеним (PA01 ΔwspF) рівнями циклічного дигуанозинмонофосфату. Культивування проводили у24-лункових плоскодонних планшетах Nuclon при 37 °C у середовищі LB. Масу біоплівки визначали у СV-тесті. Вимірювання здійснювали на спектрофотометрі SmartSpecPlus (Bio-Rad, Hungary) при довжині хвилі 592 нм. Ацил-гомсеринлактони екстрагували підкисленим етилацетатом та визначали їх кількість методом ГХ/МС. Визначення вмісту цикло-ди-ГМФ проводили з використанням репортерної плазміни Seattle шляхом вимірювання інтенсивності флуоресценції клітин у біоплівках. Результати дослідження. Встановлено, що штам P. aeruginosa PA01 pJN2133, внутрішньоклітинний вміст цикло-ди-ГМФ якого є у 4 рази меншим ніж у P. aeruginosa, утворює біоплівку, маса якої у 3,5 рази нижча ніж у батьківського штаму. Штам P. aeruginosa PA01 ΔwspF перевищує P. aeruginosa PA01 за цими показниками у 1,5 рази та 33 %, відповідно. Ще більш виражені відмінності виявляються при співставленні між собою мутантних штамів. За рівнем цикло-ди-ГМФ P. aeruginosa PA01 ΔwspF переважає штам PA01 pJN2133 у 5,9 рази, а за масою біоплівки – у п’ять разів. Найбільшу кількість сигнальних молекул системи кворум сенсінг синтезує штам з низьким рівнем вторинного месенджера. Висновки. Між внутрішньоклітинним вмістом цикло-ди-ГМФ і здатністю до утворення біоплівки існує прямо-пропорційна залежність:чим вище вміст вторинного месенджера, тим більша маса біоплівки. Концентрація аутоіндукторів QS у середовищі зворотнім чином зв’язана з внутрішньоклітинним вмістом цикло-ди-ГМФ: у порівнянні з батьківським штамом підвищена у штаму з низьким вмістом вторинного месенджера і знижена у штаму з його підвищеним рівнем Ключові слова: Pseudomonas aeruginosa, quorum sensing, біоплівки, цикло-ди-ГМФ, аутоіндуктори QS","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78260466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction and adaptive capacity of pittosporum tenuifolium gaertn. in conditions of protected soil","authors":"L. Boyko","doi":"10.15587/2519-8025.2019.179207","DOIUrl":"https://doi.org/10.15587/2519-8025.2019.179207","url":null,"abstract":"","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74701856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of anexpress-method for influence and genotyping of H1N1 and H7N9 virus avian influenza a strains by PCR-RFLP analysis","authors":"S. Buriachenko, B. Stegniy","doi":"10.15587/2519-8025.2019.179191","DOIUrl":"https://doi.org/10.15587/2519-8025.2019.179191","url":null,"abstract":"Epizootic monitoring in recent years suggests that the highly pathogenic avian influenza A virus (H1N1) and (H7N9) actively circulate in the Eurasian countries. By 2016 - 2019 1.6 thousand outbreaks were recorded. For 2016 - 2019, 1.6 thousand cases of outbreaks were recorded. Of these, there are 872 cases in Europe. The monitoring of infected birds, both migratory and poultry, in places of cross-contact in Ukraine is relevant for preventing outbreaks of epizooties. The aim of the study. To develop an express method for the identification and determination of bird flu virus A H1N1 and H7N9 strains, based on a polymerase chain reaction with analysis of restriction fragment length polymorphism (PCR-RFLP) of the virus RNA. Results and discussion. The in silico analysis of the HA, NA, and NP gene amplicons allowed in silico to calculate the primers to the variable loci of the investigated genes, to calculate the reaction conditions, to determine restriction sites for the restriction enzyme to obtain theoretical PCR electrophoregrams. An express method for the detection and identification of influenza A H1N1 and H7N9 virus by three genes (HA, NA, and NP) of H1N1 and H7N9 RNA in polymerase chain reaction, combined with RFLP analysis, was developed. The method of rapid diagnostics is able to detect avian influenza virus A H1N1 and H7N9 and differentiate it from samples of other pathogens of viral infections of birds and animals. It was established, that the PCR-RFLP rapid diagnostic method is able to detect influenza A virus RNA of H1N1 and H7N9 strains with high sensitivity (100 % sensitivity). Conclusions. The developed method of PCR-based rapid identification, combined with RFLP analysis, makes it possible to significantly simplify the method of identification due to specific amplification of an RNA region having a polymorphic restriction site. Testing of this locus is possible by pre-PCR and restriction of the amplified fragment. The method of express - diagnosis of PLR-RFLP has been established for detecting RNA virus influenza A of high pathogenic H1N1 and H7N9 strains with high indicators of sensitivity (100 % sensitivity)","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"45 24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89460672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytogenetic effects in cancer patients lymphocytes depending on the radiation source and the locality of radiation exposure in experiment ex vivo","authors":"N. Maznyk, T. Sypko, V. Starenkiy","doi":"10.15587/2519-8025.2019.178907","DOIUrl":"https://doi.org/10.15587/2519-8025.2019.178907","url":null,"abstract":"Aims: Estimation of the cytogenetic lesions yield and their distribution among cells in donor lymphocytes of cancer patients with different tumor localizations depending on the source of radiation and the locality of radiation exposure in a therapeutically significant dose in ex vivo experiment. Methods: Cytogenetic analysis was performed in lymphocytes of 30 oncogynecological patients, lung cancer patients and head and neck cancer patients before the start of radiation treatment. Whole peripheral blood was irradiated at 2 Gy dose with a further simulation of partial body irradiation using gamma-irradiation 60 Co on the ROKUS-AM and megavolt irradiation on the linear accelerator Clinac 600C. Results of research: An increase of radiation-specific chromosome damage frequency after gamma- and megavolt irradiation of cancer patients’ lymphocytes at 2 Gy dose was shown. With the absence of dependence on the tumor localization the statistically significant excess of the chromosome exchanges level due to irradiation on linear accelerator in compare with gamma-irradiation was found. At 2 Gy dose point with a simulation of partial body irradiation a similar dependence on the applied source was observed. So, the increase of the chromosome type aberrations level was due to 2,5-fold increase of the dicentric and ring chromosomes number under the gamma-irradiation and 5-fold under megavolt irradiation. For local irradiation simulation for both sources the chromosome aberrations level significantly exceeded the values of the zero point, and the dicentrics distribution among cells was over-dispersed according to Poisson statistic. Conclusion: Cytogenetic studies in ex vivo experiment showed that in donors’ lymphocytes, regardless of the tumor location, megavolt irradiation demonstrated a more genotoxic effect in compare with gamma-irradiation. The data obtained indicated that the proposed test experiment of ex vivo irradiation with partial body radiation simulation can be successfully used to detect the fact of irradiation and to confirm, if present, its locality. The study results will contribute to the improvement of the radiobiological basis of cancer patients’ radiation treatment and can be of use for the development of approaches to the individualization of therapeutic irradiation","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75236756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}