Scienceasia最新文献_第2页

miR-218-5p regulates the phenotypic transformation of vascular smooth muscle cells in rat intracranial aneurysms by targeting HMGB1 miR-218-5p通过靶向HMGB1调控大鼠颅内动脉瘤血管平滑肌细胞的表型转化

4区综合性期刊

Scienceasia Pub Date : 2024-01-01 DOI: 10.2306/scienceasia1513-1874.2024.095

Jie Peng, Jieqiong Li

引用次数: 0

Galangin modulation of the IL-23/IL-17 axis mitigates ulcerative colitis through attenuation of oxidative/nitrative stress and inflammation 高良姜调节IL-23/IL-17轴通过抑制氧化/硝化应激和炎症减轻溃疡性结肠炎

4区综合性期刊

Scienceasia Pub Date : 2024-01-01 DOI: 10.2306/scienceasia1513-1874.2024.041

Yang Zhang, Ling Xiao, Limin Yang

{"title":"Galangin modulation of the IL-23/IL-17 axis mitigates ulcerative colitis through attenuation of oxidative/nitrative stress and inflammation","authors":"Yang Zhang, Ling Xiao, Limin Yang","doi":"10.2306/scienceasia1513-1874.2024.041","DOIUrl":"https://doi.org/10.2306/scienceasia1513-1874.2024.041","url":null,"abstract":"The pathophysiology of Ulcerative Colitis (UC) involves the imbalance of pro-inflammatory cytokines, including interleukin (IL)-23 and IL-17, which play a crucial role in the development and progression of UC.Galangin (Gal), a natural flavonoid compound, has been shown to possess anti-inflammatory, antioxidant, and immunomodulatory properties.Therefore, this study aimed to investigate the potential therapeutic effects of Gal on a mouse model of dextran sulfate sodium (DSS)-induced UC by targeting the IL-23/IL-17 axis.Forty male C57BL/6 mice were randomly divided into four groups and assessed clinical and histopathological features.The colon tissues were collected for protein analysis using Western Blotting.Also, ELISA and colorimetric analysis were used to measure cytokines and oxidative/nitrative stress markers, respectively.The expressions of iNOS and COX-2 were measured by real-time quantitative PCR (RT-qPCR).We found that Gal treatment significantly attenuated the severity of UC, as evidenced by the improvement of clinical symptoms, histopathology, and reduced levels of proinflammatory cytokines, including IL-23 (fold change: 0.64; p < 0.05) and IL-17 (fold change: 0.56; p < 0.05).Moreover, Gal treatment inhibited the activation of the NF-κB pathway.Furthermore, we demonstrated that Gal treatment significantly suppressed oxidative/nitrative stress by reducing the expression levels of iNOS (fold change: 0.46; p < 0.01) and COX-2 (fold change: 0.52; p < 0.01), the two key enzymes involved in the production of reactive oxygen/nitrogen species (ROS/RNS), and increasing the activity of antioxidant enzymes, including superoxide dismutase and glutathione peroxidase.Our findings suggested that Gal targeting the IL-23/IL-17 axis improves UC by suppressing oxidative/nitrative stress and inflammation.","PeriodicalId":21577,"journal":{"name":"Scienceasia","volume":"50 2","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scienceasia.org/2024.50.n2/scias50_2024041.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147909230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Role and mechanism of FDFT1 in regulating ferroptosis in colon cancer cells via the Nrf2/GPX4 pathway FDFT1通过Nrf2/GPX4通路调控结肠癌细胞铁凋亡的作用及机制

4区综合性期刊

Scienceasia Pub Date : 2024-01-01 DOI: 10.2306/scienceasia1513-1874.2024.038

Lin Mo, Xiaolin Wang, Xin Liu, Jian Gao, Chao Tan, Shunhai Jian

{"title":"Role and mechanism of FDFT1 in regulating ferroptosis in colon cancer cells via the Nrf2/GPX4 pathway","authors":"Lin Mo, Xiaolin Wang, Xin Liu, Jian Gao, Chao Tan, Shunhai Jian","doi":"10.2306/scienceasia1513-1874.2024.038","DOIUrl":"https://doi.org/10.2306/scienceasia1513-1874.2024.038","url":null,"abstract":"This study was to investigate the role of FDFT1 in colon cancer SW480 cells and its potential molecular mechanism.The survival prognosis of colon cancer patients was analyzed by TCGA database.FDFT1 mRNA expression in FHC, SW480, HCT116, and HT29 was analyzed by RT-qPCR.FDFT1 was overexpressed using pcDNA-FDFT1.Cell viability was measured by the CCK-8.Flow cytometry was used to detect cell death and ROS.Iron ion and LPO were also measured.The ultrastructure of SW480 cells was detected by TEM.The cellular behavior of SW480 was detected by wound healing and transwell invasion.GPX4 was detected by immunofluorescence.Western blot analysis was performed to detect FDFT1, Nrf2, HO-1, and GPX4.The results showed that FDFT1 was a positive factor in the prognosis of colon cancer.pcDNA-FDFT1 could lead to an increase in iron ion content and LPO in SW480.pcDNA-FDFT1 and erastin inhibited the proliferation of SW480 and affected ROS and mitochondrial structure.The ferroptosis inhibitor lip-1 inhibited the regulation of cell viability and ROS by pcDNA-FDFT1.pcDNA-FDFT1 and erastin inhibited the expression of Nrf2, HO-1, and GPX4.The Nrf2 agonist oltipraz reversed the effects of pcDNA-FDFT1 and restored the proliferation, migration, and invasive ability of SW480.The inhibition of Nrf2, HO-1, and GPX4 by pcDNA-FDFT1 was also up-regulated by oltipraz.In conclusion, up-regulation of FDFT1 induced iron death in SW480 cells through the Nrf2/GPX4 axis and was a positive factor in the prognosis of colon cancer.","PeriodicalId":21577,"journal":{"name":"Scienceasia","volume":"50 2","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scienceasia.org/2024.50.n2/scias50_2024038.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

11β- hydroxysteroid dehydrogenase type 1 selective inhibitor alleviates insulin resistance and non-alcoholic fatty liver disease in db/db mice 11β-羟基类固醇脱氢酶1型选择性抑制剂减轻db/db小鼠胰岛素抵抗和非酒精性脂肪性肝病

4区综合性期刊

Scienceasia Pub Date : 2024-01-01 DOI: 10.2306/scienceasia1513-1874.2024.076

Wenwen Qi, Yong Liu, Pengfei Liu, Mei Yi, Chao Zhang, Shuang Zhang, Liyong Zhong

{"title":"11β- hydroxysteroid dehydrogenase type 1 selective inhibitor alleviates insulin resistance and non-alcoholic fatty liver disease in db/db mice","authors":"Wenwen Qi, Yong Liu, Pengfei Liu, Mei Yi, Chao Zhang, Shuang Zhang, Liyong Zhong","doi":"10.2306/scienceasia1513-1874.2024.076","DOIUrl":"https://doi.org/10.2306/scienceasia1513-1874.2024.076","url":null,"abstract":"Non-alcoholic fatty liver disease, often linked to insulin resistance, is associated with increased expression of 11-hydroxysteroid dehydrogenase type 1 (11-HSD1) in liver tissue.We here assessed the effects of the 11-HSD1 inhibitor BVT.2733 on non-alcoholic fatty liver disease and its underlying mechanisms.Mice were divided into three groups: control, db/db, and db/db +BVT.2733.After 4 weeks, serological testing was conducted, and glucose tolerance and insulin sensitivity were assessed.The severity of non-alcoholic fatty liver disease was evaluated using B-ultrasound, Hematoxylin and Eosin staining, and Oil Red O staining.Liver corticosterone levels were measured using enzyme-linked immunosorbent assay.Liver expression of 11-HSD1, Phosphatidylinositol 3-kinase (PI3K) -Serine-Threonine kinase (AKT)-Mammalian target of rapamycin (mTOR) signaling pathway-related proteins, and autophagy-related proteins were analyzed by western blot.The results indicated that the leptin receptor-deficient mice db/db showed significant glucolipid metabolism disorder, insulin resistance, and increased non-alcoholic fatty liver disease severity, compared with the control group.BVT.2733 treatment ameliorated these conditions.Specifically, BVT.2733 downregulated liver 11-HSD1 expression, resulting in reduced liver corticosterone levels.Additionally, BVT.2733 enhanced hepatocyte autophagy, activated the PI3K-AKT signaling pathway and inhibited the mTOR signaling pathway.These findings suggest that BVT.2733 not only improves glucolipid metabolism and insulin resistance but also mitigates non-alcoholic fatty liver disease, in leptin receptor-deficient mice db/db.The underlying mechanism likely involves decreased liver corticosterone levels and enhanced hepatocyte autophagy, mediated by the PI3K-AKT and mTOR signaling pathways.","PeriodicalId":21577,"journal":{"name":"Scienceasia","volume":"50 4","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"http://www.scienceasia.org/2024.50.n4/scias50_2024076.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147921292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cell adhesion molecule 2 inhibits colorectal cancer progression through attenuating epithelial-mesenchymal transition 细胞粘附分子2通过减弱上皮-间质转化抑制结直肠癌进展

4区综合性期刊

Scienceasia Pub Date : 2024-01-01 DOI: 10.2306/scienceasia1513-1874.2024.070

Yangyang Liu, Daoquan Fang, Xiaojuan Fang, Zuyue Zhong, Qiongying Zhang, Yichu Lian, Fanggui Shao, Lei Jiang

引用次数: 0

Nobiletin inhibits cell proliferation and invasion via suppression of UBE2C in hepatocellular carcinoma 诺比列素通过抑制肝癌细胞UBE2C抑制细胞增殖和侵袭

4区综合性期刊

Scienceasia Pub Date : 2024-01-01 DOI: 10.2306/scienceasia1513-1874.2024.088

Jinzhan Su, Shufeng Fan, Dongying Su, Miaoer Li, Xia Song

引用次数: 0

ZIF-8, chitosan and β-cyclodextrin-incorporated ZIF-8 nanohybrid for improving drug delivery ZIF-8、壳聚糖和β-环糊精复合ZIF-8纳米杂化物改善给药效果

4区综合性期刊

Scienceasia Pub Date : 2023-01-01 DOI: 10.2306/scienceasia1513-1874.2023.078

Weenawan Somphon, Arthorn Loisruangsin

引用次数: 0

Archidendron jiringa seed peel extract in the removal of lead from synthetic residual water using coagulation-flocculation process 鹅掌楸籽皮提取物混凝絮凝法去除合成废水中的铅

IF 1.2 4区综合性期刊

Scienceasia Pub Date : 2023-01-01 DOI: 10.2306/scienceasia1513-1874.2022.135

Sarah Nadira Hurairah, Nur Syaza Mohamad Fahimi, Azhar Abdul Halim, M. Mohd Hanafiah, N. Nordin, Nurul Ain Ab Jalil, Z. Daud

{"title":"Archidendron jiringa seed peel extract in the removal of lead from synthetic residual water using coagulation-flocculation process","authors":"Sarah Nadira Hurairah, Nur Syaza Mohamad Fahimi, Azhar Abdul Halim, M. Mohd Hanafiah, N. Nordin, Nurul Ain Ab Jalil, Z. Daud","doi":"10.2306/scienceasia1513-1874.2022.135","DOIUrl":"https://doi.org/10.2306/scienceasia1513-1874.2022.135","url":null,"abstract":": Archidendron jiringa seed peel extract was used to aid the coagulation-ﬂocculation process to ultimately remove lead from synthetic residual water. The effectiveness of this method was studied to obtain an alternative approach that is easy to be handled with low cost and energy in removing the lead from residual water. Optimum parameters were analyzed to determine the effectiveness of lead removal, including pH, alum dose, and A. jiringa seed peel extract dose. A study on the coagulation-ﬂocculation process with and without the aid of A. jiringa was also conducted. The optimum pH, the alum dose, and the A. jiringa seed peel extract dose were 9.0, 2.44 g / l, and 60.2 mg / l, respectively. The percentage of lead removal with the aid of A. jiringa seed peel extract was 79%, and the percentage was dropped to only 47% without the extract. A signiﬁcantly higher rate in the coagulation-ﬂocculation process due to the presence of A. jiringa seed peel extract proved its effectiveness in removing lead from wastewater.","PeriodicalId":21577,"journal":{"name":"Scienceasia","volume":"1 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68621411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Symbiotic microbiota: A class of potent immunomodulators 共生菌群:一类有效的免疫调节剂

IF 1.2 4区综合性期刊

Scienceasia Pub Date : 2023-01-01 DOI: 10.2306/scienceasia1513-1874.2022.139

Shalini Sharma, K. Chaubey, S. V. Singh, Saurabh Gupta

{"title":"Symbiotic microbiota: A class of potent immunomodulators","authors":"Shalini Sharma, K. Chaubey, S. V. Singh, Saurabh Gupta","doi":"10.2306/scienceasia1513-1874.2022.139","DOIUrl":"https://doi.org/10.2306/scienceasia1513-1874.2022.139","url":null,"abstract":"Microbiome is known to exist as symbiotic commensals in humans, domestic and wild animals, birds, fishes, reptiles, insects etc. DNA sequencing and metagenomic platforms have deciphered the complex role played by communities of microbiota (bacteria, fungi, viruses, protozoa and other eukaryotic species) in survival and regulation of host physiology, metabolism and regulation of host immune system. Any alteration in the microbial population or breach in the symbiotic alliance with the host may ultimately lead to development of different kinds of pathologies. Realization of the enormous role played by the microbiome in health and diseases of human and domestic livestock led researchers to find ways to modulate these resident microbiomes for improvement in health and management of diseases. Theoretically there are several ways that can be employed for manipulating the composition and functional capacity of the resident microbiome, which may lead to improvements in human and livestock health. Though studies have shown therapeutic potential of the microbiome, considerable challenges exist in the actual implementation of these strategies in clinical settings. This review discusses the symbiotic relationship between microbiome and host and strategies to modulate host immune responses by manipulating microbiome profile. Paper also highlights how to overcome existing obstacles for successful implementation of microbiome manipulation techniques. In this era of COVID-19, it would be worth analysing the role of resident microbiome in the magnitude of COVID-19 severity which may have occurred through immunomodulation.","PeriodicalId":21577,"journal":{"name":"Scienceasia","volume":"1 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68622086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Inequalities for m-polynomial exponentially s-type convex functions in fractional calculus 分数微积分中m-多项式指数s-型凸函数的不等式

IF 1.2 4区综合性期刊

Scienceasia Pub Date : 2023-01-01 DOI: 10.2306/scienceasia1513-1874.2022.147

P. Mohammed, A. Kashuri, Bahaaeldin Abdalla, Y. S. Hamed, K. M. Abualnaja

引用次数: 0