4区综合性期刊

Scienceasia Pub Date : 2026-01-01 DOI: 10.2306/scienceasia1513-1874.2026.025

Shuwei Li, Meitong Li, Jinmi Wu, Xuening Fei, Jinjun Yang, Yuhong Xie, Huimin Huo

引用次数: 0

Circular RNA ARF3 attenuates PM2.5-induced inflammatory response in human bronchial epithelial cells by regulating microRNA-509-3p and chromodomain helicase DNA binding protein 9 环状RNA ARF3通过调控microRNA-509-3p和染色质结构域解旋酶DNA结合蛋白9，减弱pm2.5诱导的人支气管上皮细胞炎症反应

4区综合性期刊

Scienceasia Pub Date : 2025-01-01 DOI: 10.2306/scienceasia1513-1874.2025.057

Zhu Zhou, YiMei Wang, Xiang Huang, Liwen Zhang, Di Wu, Yi Ren, Fei Lu, XiKun Gao

{"title":"Circular RNA ARF3 attenuates PM2.5-induced inflammatory response in human bronchial epithelial cells by regulating microRNA-509-3p and chromodomain helicase DNA binding protein 9","authors":"Zhu Zhou, YiMei Wang, Xiang Huang, Liwen Zhang, Di Wu, Yi Ren, Fei Lu, XiKun Gao","doi":"10.2306/scienceasia1513-1874.2025.057","DOIUrl":"https://doi.org/10.2306/scienceasia1513-1874.2025.057","url":null,"abstract":"The present study deciphered the molecular mechanisms by which circARF3 attenuates particulate matter 2.5 (PM 2.5 )-induced bronchial epithelial cell damage.Bronchial epithelial injury was modeled by PM 2.5 exposure.To regulate circARF3, microRNA-509-3p (miR-509-3p) and Chromodomain Helicase DNA Binding Protein 9 (CHD9), plasmid transfection and oligonucleotide techniques were used.Cell viability was quantified by Cell Counting Kit-8 assay.Apoptosis rate was determined by flow cytometry, while apoptotic genes were assessed by quantitative real-time polymerase chain reaction.Reactive oxygen levels were measured using a 2 ,7 -dichlorofluorescein diacetate fluorescent probe.Levels of the inflammatory cytokines were assessed by enzyme-linked immunosorbent assay, and CHD9, Toll-Like Receptor 4, and phosphorylated nuclear factor-kappa B subunit p65 were measured by Western blotting analysis.Targeted interactions between miR-509-3p, circARF3 and CHD9 were verified using dual luciferase reporter and RNA immunoprecipitation assays.Exposure to PM 2.5 decreased bronchial epithelial cell viability, increased apoptosis, and elevated ROS while activating the TLR4/NF-B pathway.CircARF3 overexpression or knockdown inhibited and promoted PM 2.5 -induced injury, respectively.Overexpressing miR-509-3p exacerbated PM 2.5 -induced injury, whereas overexpressing CHD9 showed the opposite effect.CircARF3 competitively bound miR-509-3p to mediate CHD9 expression.CircARF3 attenuates PM 2.5 -induced damage to bronchial epithelial cells by regulating miR-509-3p and promoting CHD9 expression.","PeriodicalId":21577,"journal":{"name":"Scienceasia","volume":"51 4","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"http://www.scienceasia.org/2025.51.n4/scias51_2025057.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147887625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FOXP3 influences recruitment and polarization of macrophages via regulating CCL5 in non-small cell lung cancer FOXP3在非小细胞肺癌中通过调节CCL5影响巨噬细胞的募集和极化

4区综合性期刊

Scienceasia Pub Date : 2025-01-01 DOI: 10.2306/scienceasia1513-1874.2025.021

Lamei Li, Yingyu Yang, Junkai Zhang, Xinyi Wang, Zhihua Ye

{"title":"FOXP3 influences recruitment and polarization of macrophages via regulating CCL5 in non-small cell lung cancer","authors":"Lamei Li, Yingyu Yang, Junkai Zhang, Xinyi Wang, Zhihua Ye","doi":"10.2306/scienceasia1513-1874.2025.021","DOIUrl":"https://doi.org/10.2306/scienceasia1513-1874.2025.021","url":null,"abstract":"Forkhead box P3 (FOXP3) plays a key role in the malignant progression of non-small cell lung cancer (NSCLC).However, the regulatory effect of FOXP3 on the biological activity of macrophages in NSCLC has not yet been reported.In the present study, we explored the effects of FOXP3 on the polarization and recruitment of macrophages induced by A549 and H1975 cells.RAW264.7 cells were used as macrophages.The transcription and protein levels of FOXP3, chemokine (C-C motif) ligand 5 (CCL5), and other cytokines were evaluated by reversetranscription quantitative polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA), respectively.Chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays were performed to confirm the regulatory relationship between FOXP3 and CCL5.Immunofluorescence staining was used to detect the expression of differentiation 206 (CD206) in the macrophages.The capacity of cancer cells to recruit macrophages was evaluated using a macrophage chemotaxis assay.Downregulation of FOXP3 expression inhibits macrophage recruitment by lung cancer cells.Low FOXP3 expression also decreased the expression levels of M2 macrophage markers (CD206, CD163, and interleukin-10 (IL-10)) in macrophages.Inhibition of FOXP3 expression downregulates CCL5 transcription in A549 and H1975 cells.The promotion of macrophage chemotaxis and M2 polarization in lung cancer cells overexpressing FOXP3 was reversed by downregulation of CCL5.Our study revealed that FOXP3 promoted chemotaxis and M2 polarization of macrophages in NSCLC.This effect is caused by the regulation of CCL5 secretion by cancer cells.","PeriodicalId":21577,"journal":{"name":"Scienceasia","volume":"51 2","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scienceasia.org/2025.51.n2/scias51_2025021.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147882909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AS-1 attenuates the inflammatory response by targeting AKT and MAPK in LPS-activated acute lung injury models

4区综合性期刊

Scienceasia Pub Date : 2025-01-01 DOI: 10.2306/scienceasia1513-1874.2025.048

Fuling Cen, Yan-Mei Wei, Xuan Zhang, Hanmeng Liu, Guang‐Ying Chen

{"title":"AS-1 attenuates the inflammatory response by targeting AKT and MAPK in LPS-activated acute lung injury models","authors":"Fuling Cen, Yan-Mei Wei, Xuan Zhang, Hanmeng Liu, Guang‐Ying Chen","doi":"10.2306/scienceasia1513-1874.2025.048","DOIUrl":"https://doi.org/10.2306/scienceasia1513-1874.2025.048","url":null,"abstract":"We investigated the effects of 3,28-dihydroxyolean-12-ene (AS-1) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in Balb/c mice and attempted to elucidate its underlying mechanism of action.The levels of pro-inflammatory cytokines such as tumor necrosis factor- (TNF-), interleukin-6 (IL-6), and interleukin-1 (IL-1) were measured using an ELISA kit.Furthermore, we analyzed the protein expression levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and the proteins involved in the kinase B/mitogen-activated protein kinase (AKT/MAPK) signaling pathways by Western blot analysis.Additionally, we evaluated AS-1 for toxicity and teratogenicity using the Cell Counting Kit-8 (CCK-8) assay and the zebrafish embryo toxicity assay.The results revealed that AS-1 suppressed the expression of pro-inflammatory cytokines and the phosphorylation of MAPK-related proteins, while enhancing the phosphorylation of AKT-related proteins.Furthermore, AS-1 was found to be non-teratogenic at concentrations below 12.5 M.These findings suggest that AS-1 may alleviate ALI by modulating the AKT/MAPK signaling pathway.","PeriodicalId":21577,"journal":{"name":"Scienceasia","volume":"51 3","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scienceasia.org/2025.51.n3/scias51_2025048.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147899572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of physicochemical characteristics of Acer truncatum seed oil based on three extraction methods

4区综合性期刊

Scienceasia Pub Date : 2024-01-01 DOI: 10.2306/scienceasia1513-1874.2024.022

Ningxia Xu, Pei Wang, Ling Chen, Qingyi Li, Zhanbin Guo, Ying Zha, Ning Cui

引用次数: 1

Prokaryotic expression of the Mycobacterium tuberculosis PhoY2 gene and its diagnostic utility in various populations infected with tuberculosis

4区综合性期刊

Scienceasia Pub Date : 2024-01-01 DOI: 10.2306/scienceasia1513-1874.2024.082

Jingyan Zhang, Qiangsen Zhong, Xiaochun Wang

{"title":"Prokaryotic expression of the Mycobacterium tuberculosis PhoY2 gene and its diagnostic utility in various populations infected with tuberculosis","authors":"Jingyan Zhang, Qiangsen Zhong, Xiaochun Wang","doi":"10.2306/scienceasia1513-1874.2024.082","DOIUrl":"https://doi.org/10.2306/scienceasia1513-1874.2024.082","url":null,"abstract":"Early and timely diagnosis is crucial for reducing the transmission of tuberculosis.In this study, we constructed and expressed PhoY2, a persistent infection related antigen of Mycobacterium tuberculosis and evaluated its potential as a diagnostic target through population experiments.The immunogenicity of the recombinant PhoY2 (rPhoY2) protein in M. tuberculosis infected individuals was evaluated using whole blood interferon-gamma (IFN-) release assay (WBIA) and serum specific antibody detection.Further, the diagnostic value, sensitivity, and specificity of rPhoY2-WBIA and anti-rPhoY2 specific antibody in different M. tuberculosis infected populations were analyzed using receiver operating characteristic.The results indicated that rPhoY2 can stimulate specific IFN- production in active tuberculosis (ATB) and latent tuberculosis infection (LTBI), levels of which were significantly higher than that in healthy controls (HCs).The anti-rPhoY2 specific IgG levels of LTBI were significantly higher than those of ATB and HCs.The cutoff value of rPhoY2-WBIA for diagnosing M. tuberculosis infection is 309.2 pg/ml with a sensitivity of 72.2% and a specificity of 93.7%.The cutoff value of anti-rPhoY2 specific IgG for diagnosing LTBI is 0.1765 with 61.5% sensitivity and 75% specificity.The agreement rate between rPhoY2-WBIA combined with specific antibodies and clinical diagnosis for the three populations is 69.9%.In summary, the combination of rPhoY2-WBIA and specific antibody detection has certain value in distinguishing different populations infected with M. tuberculosis, especially in excluding the infection with high specificity.However, its sensitivity should be further enhanced for differentiating ATB and LTBI.","PeriodicalId":21577,"journal":{"name":"Scienceasia","volume":"50 4","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"http://www.scienceasia.org/2024.50.n4/scias50_2024082.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147900500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of mesenchymal stem cells on ovarian sirtuin 1 and caspase-3 in premature ovarian insufficiency mice

4区综合性期刊

Scienceasia Pub Date : 2024-01-01 DOI: 10.2306/scienceasia1513-1874.2024.093

Yulan He, Yuan Yang, Ting Liao, Weiwei Xie, Shihuan Tang, Hong-mei Zhang, Peng Lü, Pei‐Ling Wu, Hui Zhang, Ting Long, Chun-bing Zheng, Xianping Zhang

{"title":"Effects of mesenchymal stem cells on ovarian sirtuin 1 and caspase-3 in premature ovarian insufficiency mice","authors":"Yulan He, Yuan Yang, Ting Liao, Weiwei Xie, Shihuan Tang, Hong-mei Zhang, Peng Lü, Pei‐Ling Wu, Hui Zhang, Ting Long, Chun-bing Zheng, Xianping Zhang","doi":"10.2306/scienceasia1513-1874.2024.093","DOIUrl":"https://doi.org/10.2306/scienceasia1513-1874.2024.093","url":null,"abstract":"Human umbilical cord mesenchymal stem cells (HUCMSCs) can promote angiogenesis and reduce apoptosis, but their effects in premature ovarian insufficiency (POI) remain unclear.We found that HUCMSCs could improve ovarian function in POI mice through sirtuin 1 (SIRT1)/caspase-3.Female C57BL/6 mice (n = 45) were randomly divided into 3 groups: the POI and HUCMSC groups receiving cyclophosphamide for 14 days to induce POI and the control group receiving normal saline injections.HUCMSCs were injected into the tail veins of the mice in the HUCMSC group on days 14 and 28.We analyzed the mouse body and ovarian weight, ovarian structure, number of ovarian follicles, estrous cycles, serum hormone levels, ovarian SIRT1 and caspase-3 expression, and granulosa-cell apoptosis.The POI group showed decreased body weight, ovarian weight, number of follicles, and SIRT1 expression as well as impaired ovarian hormones, and increased caspase-3 and granulosa-cell apoptosis.Body weight, ovarian weight, disturbed estrous cycle, number of follicles, and hormone levels were improved after the HUCMSC intervention, while the ovarian SIRT1 level was increased, and caspase-3 and granulosa-cell apoptosis were decreased.HUCMSCs promote SIRT1 expression and downregulate caspase-3, thereby reducing ovarian granulosa-cell apoptosis and improving ovarian function in POI mice.","PeriodicalId":21577,"journal":{"name":"Scienceasia","volume":"50 5","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"http://www.scienceasia.org/2024.50.n5/scias50_2024093.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147896778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

miR-218-5p regulates the phenotypic transformation of vascular smooth muscle cells in rat intracranial aneurysms by targeting HMGB1 miR-218-5p通过靶向HMGB1调控大鼠颅内动脉瘤血管平滑肌细胞的表型转化

4区综合性期刊

Scienceasia Pub Date : 2024-01-01 DOI: 10.2306/scienceasia1513-1874.2024.095

Jie Peng, Jieqiong Li

引用次数: 0

Role and mechanism of FDFT1 in regulating ferroptosis in colon cancer cells via the Nrf2/GPX4 pathway

4区综合性期刊

Scienceasia Pub Date : 2024-01-01 DOI: 10.2306/scienceasia1513-1874.2024.038

Lin Mo, Xiaolin Wang, Xin Liu, Jian Gao, Chao Tan, Shunhai Jian

{"title":"Role and mechanism of FDFT1 in regulating ferroptosis in colon cancer cells via the Nrf2/GPX4 pathway","authors":"Lin Mo, Xiaolin Wang, Xin Liu, Jian Gao, Chao Tan, Shunhai Jian","doi":"10.2306/scienceasia1513-1874.2024.038","DOIUrl":"https://doi.org/10.2306/scienceasia1513-1874.2024.038","url":null,"abstract":"This study was to investigate the role of FDFT1 in colon cancer SW480 cells and its potential molecular mechanism.The survival prognosis of colon cancer patients was analyzed by TCGA database.FDFT1 mRNA expression in FHC, SW480, HCT116, and HT29 was analyzed by RT-qPCR.FDFT1 was overexpressed using pcDNA-FDFT1.Cell viability was measured by the CCK-8.Flow cytometry was used to detect cell death and ROS.Iron ion and LPO were also measured.The ultrastructure of SW480 cells was detected by TEM.The cellular behavior of SW480 was detected by wound healing and transwell invasion.GPX4 was detected by immunofluorescence.Western blot analysis was performed to detect FDFT1, Nrf2, HO-1, and GPX4.The results showed that FDFT1 was a positive factor in the prognosis of colon cancer.pcDNA-FDFT1 could lead to an increase in iron ion content and LPO in SW480.pcDNA-FDFT1 and erastin inhibited the proliferation of SW480 and affected ROS and mitochondrial structure.The ferroptosis inhibitor lip-1 inhibited the regulation of cell viability and ROS by pcDNA-FDFT1.pcDNA-FDFT1 and erastin inhibited the expression of Nrf2, HO-1, and GPX4.The Nrf2 agonist oltipraz reversed the effects of pcDNA-FDFT1 and restored the proliferation, migration, and invasive ability of SW480.The inhibition of Nrf2, HO-1, and GPX4 by pcDNA-FDFT1 was also up-regulated by oltipraz.In conclusion, up-regulation of FDFT1 induced iron death in SW480 cells through the Nrf2/GPX4 axis and was a positive factor in the prognosis of colon cancer.","PeriodicalId":21577,"journal":{"name":"Scienceasia","volume":"50 2","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scienceasia.org/2024.50.n2/scias50_2024038.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZIF-8, chitosan and β-cyclodextrin-incorporated ZIF-8 nanohybrid for improving drug delivery ZIF-8、壳聚糖和β-环糊精复合ZIF-8纳米杂化物改善给药效果

4区综合性期刊

Scienceasia Pub Date : 2023-01-01 DOI: 10.2306/scienceasia1513-1874.2023.078

Weenawan Somphon, Arthorn Loisruangsin

引用次数: 0

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