Saudi Journal of Biological Sciences最新文献

{"title":"Controlling of multidrug resistant Aeromonas hydrophila infected Nile tilapia (Oreochromis niloticus) using Ah01 and Ah02 virulent bacteriophages isolates","authors":"Nahed A. El-Wafai ,&nbsp;Nada K. Alharbi ,&nbsp;Ahmed Ezzat Ahmed ,&nbsp;Fatma I. El-Zamik ,&nbsp;Samir A. Mahgoub ,&nbsp;Alaa M.S. Atia ,&nbsp;Eman A.A. Abdel-Hamid","doi":"10.1016/j.sjbs.2022.02.050","DOIUrl":"10.1016/j.sjbs.2022.02.050","url":null,"abstract":"<div><div>The aim of this work is to investigate the efficiency Ah01 and Ah02 virulent bacteriophages isolated from fish in controlling <em>Aeromonas hydrophila-</em>infected Nile talipa fish. <em>Aeromonas hydrophila</em> strains were resistant to 64% of tested antibiotic, where the antibiotic sensitivity test revealed that among 28 different antibiotics, <em>Aeromonas hydrophila</em> (ATCC 13037) was resistant to 18 antibiotics. In this study, two lytic phages designated as Ah01 and Ah02 specific to <em>Aeromonas hydrophila</em> strain (ATCC 13037) were isolated from fish and agricultural irrigation water, respectively. The isolated phages exhibited tolerance to alkaline and acidic reactions (pH 3–12). The thermal inactivation point was found to be 84 °C for both phages (Ah01 and Ah02). Longevity <em>in vitro</em> of Ah01 and Ah02 phages was estimated to be 21 days at 22 ± 2 °C. The two phage isolates were polyvirulent, since, they were infectious to different species of genus <em>Aeromonas</em>. Transmission electron microscopy analysis revealed that Ah01 and Ah02 phages are of hexagonal head and contractile tail type. Therefore, these two phages belong to family <em>Myoviridae</em>. One step growth curve indicated that the latent periods were15 min for both phages (Ah01 and Ah02). The burst sizes were 102 and 209 PFU/ cell for Ah01 and Ah02 phages, respectively. The RAPD-PCR was used to study the genetic diversity and distances of DNA products of the two virulent phages (Ah01 &amp; Ah02). Six primers with different sizes (P13, N8, B12, A01, S50 and S8) were succeeded to reproduce polymorphic DNA bands. Genetic distances relationship among the two phages were studied based on their similarity indices data of RAPD- PCR analysis. The RAPD primers achieved a rate of polymorphism ranged between 50% and 100%. Complete mortality was recorded in fish injected with <em>Aeromonas hydrophila</em> (ATCC 13037) within two days, but when adding phages, the phage isolates were combined with <em>A. hydrophila</em> strain (ATCC 13037) and intraperitoneal injected in fish, no mortality was detected until the end of experiment (15 days).</div></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"32 ","pages":"Article 103240"},"PeriodicalIF":4.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44904823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New eco-friendly trends to produce biofuel and bioenergy from microorganisms: An updated review","authors":"Nidal M. Zabermawi ,&nbsp;Faten A.S. Alsulaimany ,&nbsp;Mohamed T. El-Saadony ,&nbsp;Khaled A. El-Tarabily","doi":"10.1016/j.sjbs.2022.02.024","DOIUrl":"10.1016/j.sjbs.2022.02.024","url":null,"abstract":"<div><div>It is critical to ensure the safe disposal of organic residues, especially because the accumulation of organic wastes contributes to environmental contamination; spread of diseases, unpleasant odors; and the release of ammonia and other dangerous gases in the environment. Consequently, researchers are considering various direct organic waste applications, including biotechnological applications with ecological and economical benefits such as the limitation of fossil fuel usage, lowering harmful emissions, boosting the synthesis of cost-effective raw materials, and establishing a suitable platform for a diversity of microorganisms. Biotechnology has produced sustainable bioenergy (biogas, biodiesel, bioethanol, and biobutanol), which is an appealing solution for the disposal of organic materials. Carbohydrates are the main component of the organic fraction, and the bulk of these polymers are easily degradable by microorganisms. Taking random samples from soils exposed to organic wastes, purifying the microbial isolates, and evaluating the microbes’ capabilities to identify the most useful strain are all part of the isolation process. As a result, this current review focuses on isolated strains of various microorganisms that may use one or more types of organic wastes as the sole carbon source, and to manufacture biofuel as a product from various residues.</div></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"32 ","pages":"Article 103214"},"PeriodicalIF":4.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45895123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of CeO2 modified cathode and PANI modified anode on tannery wastewater fed microbial fuel cell performance","authors":"Muhammad Waseem Mumtaz ,&nbsp;Hamid Mukhtar ,&nbsp;Waheed Miran ,&nbsp;Abdulrahman H. Alessa ,&nbsp;Aashir Waleed ,&nbsp;Zoha Sarwar ,&nbsp;Haseeb Ashraf","doi":"10.1016/j.sjbs.2024.104024","DOIUrl":"10.1016/j.sjbs.2024.104024","url":null,"abstract":"<div><p>Microbial fuel cell (MFC) technology is getting acceptance as an emphatic, sustainable and energy efficient alternative of conventional wastewater treatment strategies. MFCs utilize exoelectrogens as biocatalysts to degrade the complex organic substances present in wastewater with simultaneous power generation. The present study was aimed at investigating the impact of MFC electrode’s modification with CeO₂ nanoparticles and polyaniline (PANI) on its performance characteristics. The hydrothermal approach was employed for the synthesis of CeO₂ nanoparticles followed by their deposition on carbon cloth (CC) as MFC cathode, whereas MFC’s anode i.e., CF/NF was modified by in-situe deposition of PANI. The synthesized material was characterized with FTIR, XRD, SEM, EDX and BET analysis. The experiments were performed using dual chambered MFC fed with leather tannery wastewater using modified and unmodified electrodes. The highest outcomes of power density and corresponding current density were observed with PANI@NF composite anode and CeO₂@CC as cathode i.e., 279.3 mW/m² corresponding to the current density of 581.8 mA/m². The same MFC electrode configuration resulted in highest COD reduction, i.e., 80 % and coulombic efficiency of 19.86 %. On the other hand, MFC equipped with PANI@CF anode and CeO₂@CC cathode also displayed comparable results. It was ascertained that modification of NF/CF anode with PANI (conductive polymer) and CC cathode with CeO₂ nanoparticles have significantly improved the overall MFC operational performance regarding tannery wastewater treatment and bioelectricity generation.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 8","pages":"Article 104024"},"PeriodicalIF":4.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24001025/pdfft?md5=145d9979d08b5fcf23e4ad95302c17ef&pid=1-s2.0-S1319562X24001025-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141037809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deregulation of TWIST1 expression by promoter methylation in gastrointestinal cancers","authors":"","doi":"10.1016/j.sjbs.2023.103842","DOIUrl":"10.1016/j.sjbs.2023.103842","url":null,"abstract":"<div><p><em>TWIST1</em>, a basic helix-loop-helix transcription factor with versatile roles in cancer, is frequently deregulated in cancers, through established pathway perturbations. However. the significance of <em>TWIST1</em> methylation in the deregulation of <em>TWIST1</em> in gastrointestinal cancers is not fully clear. This study hypothesized that <em>TWIST1</em> promoter methylation deregulates <em>TWIST1</em> expression independent of established deregulators such as the <em>WNT</em>, <em>TGFB</em>, <em>NOTCH</em> and miRNA pathways. To prove this hypothesis, colon, gastric and rectal cancer genomic data comprising gene expression, DNA methylation, and miRNA data were retrieved from the Cancer Genome Atlas cohorts which are publicly available in cancer genomic databases, the Genome Data Commons and the <span><span>cBioportal.org</span><svg><path></path></svg></span>. About 217 variables comprising expression levels of genes of the <em>WNT</em>, <em>TGFB</em>, <em>NOTCH</em> and miRNA signalling pathways, as well as the beta values of 17 <em>TWIST1</em> methylation loci were subjected to Principal Component Regression Analysis, and then standard Linear Regression Analysis. The results showed that <em>TWIST1</em> methylation is a predictor of <em>TWIST1</em> expression in the gastrointestinal cancers, independent of <em>WNT</em>, <em>TGFB</em>, and <em>NOTCH</em> signalling and miRNA deregulation. The results also showed that different <em>TWIST1</em> methylation loci may deregulate <em>TWIST1</em> expression in different cancer types. The inference that can be drawn from this study is that <em>TWIST1</em> DNA methylation is an important <em>TWIST1</em> deregulation mechanism in colon, rectal and gastric cancers.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 8","pages":"Article 103842"},"PeriodicalIF":4.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X23002875/pdfft?md5=d89e58d838f933e0b0cfa26bac1f0542&pid=1-s2.0-S1319562X23002875-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135850188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenolic content of Thai Bao mango peel and its in-vitro antioxidant, anti-cholinesterase, and antidiabetic activities","authors":"Sirinapa Thangsiri ,&nbsp;Uthaiwan Suttisansanee ,&nbsp;Pankaj Koirala ,&nbsp;Wimonphan Chathiran ,&nbsp;Warangkana Srichamnong ,&nbsp;Li Li ,&nbsp;Nilesh Nirmal","doi":"10.1016/j.sjbs.2024.104033","DOIUrl":"https://doi.org/10.1016/j.sjbs.2024.104033","url":null,"abstract":"<div><p>Plant phenolics have been known for various biological activities. This study aims to extract and examine the presence of phenolics in Bao mango (Mangifera indica L. var.) peel ethanolic extract (MPE). Further, antioxidant, anti-diabetic (α-amylase, and α-glucosidase inhibitory activity), and anti- Alzheimer’s disease (AD) (acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and β-secretase (BACE-1) inhibitory activity) efficacy of MPE were determined. The results indicated that mangiferin (8755.89 mg/ 100 g extract) was the major phenolic compound in MPE. An antioxidant mechanism revealed that MPE had a higher radical scavenging ability (4266.70 µmol TE/g extract) compared to reducing power (FRAP) or oxygen radical absorption capacity (ORAC). Further <em>in-vitro</em> enzyme inhibitory assay against diabetic and AD involved enzymes showed that MPE had stronger inhibitory action against an enzyme involved in diabetes compared to their standard drug (Acarbose) (P &lt; 0.05). While a lower IC₅₀ value was observed against AD-involved enzymes compared to their standard drug (donepezil) (P &lt; 0.05). The results show that Thai Bao mango peel byproduct can be a potential source of nutraceuticals to lower diabetes and improve cognitive health.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 8","pages":"Article 104033"},"PeriodicalIF":4.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24001116/pdfft?md5=5d023990d089747b3d1fbb25ace2f5ef&pid=1-s2.0-S1319562X24001116-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141251032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Global Trends of Bacillus, Trichoderma and Entomopathogenic Fungi for Pathogen and Pest Control in Chili Cultivation","authors":"Muhamad Firdaus Syahmi Sam-on ,&nbsp;Shuhaimi Mustafa ,&nbsp;Mohd Termizi Yusof ,&nbsp;Amalia Mohd Hashim ,&nbsp;Ku Nur Azwa Ku Aizuddin","doi":"10.1016/j.sjbs.2024.104046","DOIUrl":"10.1016/j.sjbs.2024.104046","url":null,"abstract":"<div><p>Chili, renowned globally and deeply ingrained in various cultures. Regrettably, the onset of diseases instigated by pests and pathogens has inflicted substantial losses on chili crops, with some farms experiencing complete production decimation. Challenges confronting chili cultivation include threats from pathogenic microbes like <em>Xanthomonas, Fusarium, Phytophthora, Verticillium, Rhizoctonia, Colletotrichium</em> and Viruses, alongside pests such as whiteflies, mites, thrips, aphids, and fruit flies. While conventional farming practices often resort to chemical pesticides to combat these challenges, their utilization poses substantial risks to both human health and the environment. In response to this pressing issue, this review aims to evaluate the potential of microbe-based biological control as eco-friendly alternatives to chemical pesticides for chili cultivation. Biocontrol agents such as <em>Bacillus</em> spp., <em>Trichoderma</em> spp., and entomopathogenic fungi present safer and more environmentally sustainable alternatives to chemical pesticides. However, despite the recognised potential of biocontrol agents, research on their efficacy in controlling the array of pests and pathogens affecting chili farming remains limited. This review addresses this gap by evaluating the efficiency of biocontrol agents, drawing insights from existing studies conducted in other crop systems, regarding pest and pathogen management. Notably, an analysis of Scopus publications revealed fewer than 30 publications in 2023 focused on these three microbial agents. Intriguingly, India, as the world’s largest chili producer, leads in the number of publications concerning <em>Bacillus</em> spp., <em>Trichoderma</em> spp., and entomopathogenic fungi in chili cultivation. Further research on microbial agents is imperative to mitigate infections and reduce reliance on chemical pesticides for sustainable chili production.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 8","pages":"Article 104046"},"PeriodicalIF":4.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24001244/pdfft?md5=e10e3f39bdc1b39f0316b98f75a575d4&pid=1-s2.0-S1319562X24001244-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141392432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, pandemic, preventions and policies to overcome antimicrobial resistance","authors":"Ghallab Alotaibi","doi":"10.1016/j.sjbs.2024.104032","DOIUrl":"10.1016/j.sjbs.2024.104032","url":null,"abstract":"<div><p>Antimicrobial resistance (AMR) is a growing concern in Asia, and it is essential to understand the prevalence, pandemic, prevention, and policies to overcome it. According to the World Health Organization (WHO), AMR is one of the main causes of death; in 2019, it was linked to 4.95 million fatalities and caused about 1.27 million deaths. A core package of actions has been provided by WHO to help countries prioritize their needs when creating, carrying out, and overseeing national action plans on antimicrobial resistance. Using a people-cantered approach to AMR, the interventions address the needs and obstacles that individuals and patients encounter when trying to obtain healthcare. The people-cantered core package of AMR treatments seeks to improve public and policymakers; awareness and comprehension of AMR by changing the narrative of AMR to emphasize the needs of people and systemic impairments. Additionally, it backs a more comprehensive and programmatic national response to AMR, which emphasizes the value of fair and inexpensive access to high-quality healthcare services for the avoidance, identification, and management of drug-resistant diseases. The report signals increasing resistance to antibiotics in bacterial infections in humans and the need for better data. In conclusion, the prevalence of AMR in Asia is a significant public health concern, and it is crucial to implement policies and interventions to overcome it.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 8","pages":"Article 104032"},"PeriodicalIF":4.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24001104/pdfft?md5=787b924a4b4408bf59e224ed5e3097e0&pid=1-s2.0-S1319562X24001104-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141189794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haemonchus contortus, an obligatory haematophagus worm infection in small ruminants: Population genetics and genetic diversity","authors":"Shanaz Parvin ,&nbsp;Anita Rani Dey ,&nbsp;Nusrat Nowrin Shohana ,&nbsp;Anisuzzaman ,&nbsp;Md. Hasanuzzaman Talukder ,&nbsp;Mohammad Zahangir Alam","doi":"10.1016/j.sjbs.2024.104030","DOIUrl":"10.1016/j.sjbs.2024.104030","url":null,"abstract":"<div><p><em>Haemonchus contortus,</em> a stomach worm, is prevalent in ruminants worldwide. They particularly hamper profitable small ruminant production. Here, we estimate the genetic variation of <em>H. contortus</em> collected from slaughtered goats and sheep from various geographic zones of Bangladesh using multiple genes. To perform this, adult parasites were isolated from the abomasum of slaughtered animals (sheep and goats). Among them, 79 male <em>H. contortus</em> were identified by microscopy. Following the extraction of DNA, <em>ITS-2</em> and <em>cox1</em> genes were amplified and subsequently considered for sequencing. After alignment and editing, sequences were analyzed to find out sequence variation, diversity pattern of genes, and population genetics of isolates. Among the sequence data, the analyses identified 19 genotypes of <em>ITS-2</em> and 77 haplotypes of <em>cox1</em> genes. The diversity of nucleotides was 0.0103 for <em>ITS-2</em> and 0.029 for <em>cox1</em> gene. The dendogram constructed by the genotype and haplotype sequences of <em>H. contortus</em> revealed that two populations were circulating in Bangladesh without any demarcation of host and geographic regions. Analysis of population genetics demonstrated a high flow of genes (89.2 %) within the population of the worm in Bangladesh. The Fst value showed very little amount of genetic difference among the worm populations of Bangladesh but marked genetic variation between different continents. The findings are expected to help explain the risks of anthelmintic resistance and the transmission pattern of the parasite, and also provide a control strategy against <em>H. contortus</em>.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 8","pages":"Article 104030"},"PeriodicalIF":4.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24001086/pdfft?md5=420e3a571af458762b0bcd7d2d6adef1&pid=1-s2.0-S1319562X24001086-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141189886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How does a Saccharomyces cerevisiae extract influence the components of isolated rotavirus particles from stool samples collected in a clinical setting from children?","authors":"Mona A.M. Hussein ,&nbsp;Mayasar I. Al-zaban ,&nbsp;Yahia A.G. Mahmoud ,&nbsp;Amin A. Al-Doaiss ,&nbsp;Safia M.A. Bahshwan ,&nbsp;Khalid A. El-Dougdoug ,&nbsp;Mohamed R. EL-Shanshory","doi":"10.1016/j.sjbs.2024.104031","DOIUrl":"https://doi.org/10.1016/j.sjbs.2024.104031","url":null,"abstract":"<div><p>Human Rotavirus (HRV) is the causative pathogen of severe acute enteric infections that cause mortality among children worldwide. This study focuses on developing a new and effective treatment for rotavirus infection using an extract from Saccharomyces cerevisiae, aiming to make this treatment easily accessible to everyone. 15 antigens and 26 antibodies were detected in serum and stool using ELISA. The titers of HRVq1, HRVq2, HRVC1, and HRVC2 on Vero cells were determined to be 1.2x10⁶, 3.0x10⁶, 4.2x10⁶, and 7.5x10⁵ (Plaque forming unit, PFU/ml) four days after infection, respectively. The HRVq1 isolate induced cytopathic effects, i.e., forming multinucleated, rounded, enlarged, and expanding gigantic cells. RT-PCR identified this isolate, and the accession number 2691714 was assigned to GeneBank. The molecular docking analysis revealed that nonstructural proteins (NSPs) NSP1, NSP2, NSP3, NSP4, NSP5, and NSP6 exhibited significant binding with RNA. NSP2 demonstrated the highest binding affinity and the lowest binding energy (−8.9 kcal/mol). This affinity was maintained via hydrophobic interactions and hydrogen bonds spanning in length from 1.12 Å to 3.11 Å. The ADMET and bioactivity predictions indicated that the yeast extract possessed ideal solubility, was nontoxic, and did not cause cancer. The inhibitory constant values predicted for the <em>S. cerevisiae</em> extract in the presence of HRV vital proteins varied from 5.32 to 7.45 mM, indicating its potential as a viable drug candidate. <em>Saccharomyces cerevisiae</em> extract could be utilized as a dietary supplement to combat HRV as an alternative dietary supplement.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 8","pages":"Article 104031"},"PeriodicalIF":4.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24001098/pdfft?md5=747bf32ec92f924cbae70b4d7302d805&pid=1-s2.0-S1319562X24001098-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141251035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of 6-gingerol and semi-synthesis of 1,4-benzodiazepines derivatives: An in-situ pharmacokinetics properties, molecular docking and molecular dynamics simulation assessments","authors":"Mariyappan Vaithiyalingam ,&nbsp;Ramasamy Mohan Kumar ,&nbsp;Prerna Khagar ,&nbsp;Sarvesh Sabarathinam ,&nbsp;Yahia Alghazwani ,&nbsp;Kumarappan Chidambaram","doi":"10.1016/j.sjbs.2024.104048","DOIUrl":"https://doi.org/10.1016/j.sjbs.2024.104048","url":null,"abstract":"<div><p>This paper outlines a methodical approach for isolating 6-gingerol (1a) from <em>Zingiber officinale Roscoe</em> rhizomes on a gram-scale, resulting in a product of high purity and significant yield. Further, 6-gingerol (1a) [SSG1] derivatives, including 1-(4-hydroxy-3-methoxyphenyl)decane-3,5-dione (1ab), were synthesized via a semi-synthetic pathway involving DMP-mediated fast oxidation and replication. Subsequently, a new series of 1,4-benzodiazepines (3a-c) was synthesized quantitatively using a basic technique. This synthesis necessitated the interaction of 1ab with various o-phenylenediamine (2a-c) compounds. Spectroscopic methods were employed to characterize the synthesized 1,4-benzodiazepines (3a-c)[SSG2, SSG3 &amp; SSG4]. Despite extensive investments by pharmaceutical companies in traditional drug research and development for diseases like type 2 diabetes (T2D), successful treatments remain elusive. Medication repurposing has gained traction as a strategy to address not only diabetes but also other disorders. Leveraging existing molecular pharmacology data accelerates the development of new medications. This paper underscores the importance of repurposing traditional medicines to combat a range of communicable and non-communicable diseases, offering a promising avenue for therapeutic advancement. Additionally, molecular docking studies suggested that one derivative (SSG2) exhibited stronger binding affinity compared to the reference standards. Overall, the findings of this study highlight the potential of semi-synthetic gingerol derivatives for the development of novel therapeutic agents.</p></div>","PeriodicalId":21540,"journal":{"name":"Saudi Journal of Biological Sciences","volume":"31 8","pages":"Article 104048"},"PeriodicalIF":4.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1319562X24001268/pdfft?md5=903aa2dd1a8ddd657d0b5abad206925d&pid=1-s2.0-S1319562X24001268-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141325140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}