{"title":"Antibody-dependent cell-mediated cytotoxicity and phagocytosis of autologous red blood cells in alphamethyldopa-induced haemolysis.","authors":"I Yust, B Frisch, N Goldsher","doi":"10.1111/j.1600-0609.1986.tb00830.x","DOIUrl":"https://doi.org/10.1111/j.1600-0609.1986.tb00830.x","url":null,"abstract":"<p><p>7 alphamethyldopa (AMD)-treated patients with positive direct antiglobulin test (DAT) were investigated. Peripheral blood mononuclear phagocytes of 2 patients suffering from haemolysis caused lysis and phagocytosis of autologous DAT-positive red blood cells (RBC). Eluates from RBC of both patients contained antibodies of IgG1 subclass and supported antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent phagocytosis (ADPh) of the patients' RBC after remission. Both cytotoxic activities were proportional to the serum concentration and to the number of attacking cells. The 5 patients without overt haemolysis did not show in vitro lysis or phagocytosis of autologous RBC. These results suggest that ADCC as well as ADPh participate in the destruction of RBC in AMD-induced haemolysis in vivo.</p>","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"36 2","pages":"211-6"},"PeriodicalIF":0.0,"publicationDate":"1986-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0609.1986.tb00830.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14824924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pneumococcaemia as a presenting sign in 3 cases of multiple myeloma.","authors":"E Barasch, S A Berger, E Golan, Y Siegman-Igra","doi":"10.1111/j.1600-0609.1986.tb00833.x","DOIUrl":"https://doi.org/10.1111/j.1600-0609.1986.tb00833.x","url":null,"abstract":"<p><p>Multiple myeloma was diagnosed in 3 patients following episodes of pneumococcaemia associated with neutropenia and decreased serum concentration of normal immunoglobulins. Severe pneumococcal infection is commonly encountered during the course of multiple myeloma, but has not been stressed as a presenting feature of the disease.</p>","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"36 2","pages":"229-31"},"PeriodicalIF":0.0,"publicationDate":"1986-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0609.1986.tb00833.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14824925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M G Giudizi, R Biagiotti, F Almerigogna, M Mazzetti, A Alessi, G Massai, G Longo, G Scano, M Morfini, S Romagnani
{"title":"HTLV-III seropositivity in symptom-free Italian haemophiliacs. Correlation with consumption of commercial concentrate and abnormalities of T and B lymphocytes.","authors":"M G Giudizi, R Biagiotti, F Almerigogna, M Mazzetti, A Alessi, G Massai, G Longo, G Scano, M Morfini, S Romagnani","doi":"10.1111/j.1600-0609.1986.tb00828.x","DOIUrl":"https://doi.org/10.1111/j.1600-0609.1986.tb00828.x","url":null,"abstract":"<p><p>Various immunological parameters exploring both T- and B-cell functions were determined in a group of 74 symptom-free Italian haemophiliacs treated with commercial concentrate imported from the USA and were correlated with the presence in their serum of antibody to HTLV-III. There was a strong correlation between HTLV-III seropositivity and the amount of concentrate consumed. A significant correlation between HTLV-III seropositivity and T-cell alterations, such as T4/T8 ratio less than 1 and reduction in the absolute number of T4+ lymphocytes, or B-cell alterations such as hypergammaglobulinaemia and enhanced spontaneous IgG synthesis in vitro, was also observed.</p>","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"36 2","pages":"198-202"},"PeriodicalIF":0.0,"publicationDate":"1986-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0609.1986.tb00828.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13569554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Increased binding to ADP-stimulated platelets and aggregation effect of the dysfibrinogen Oslo I as compared with normal fibrinogen.","authors":"L I Thorsen, F Brosstad, N O Solum, H Stormorken","doi":"10.1111/j.1600-0609.1986.tb00829.x","DOIUrl":"https://doi.org/10.1111/j.1600-0609.1986.tb00829.x","url":null,"abstract":"<p><p>Interactions of the dysfibrinogen Oslo I with platelets were investigated. This fibrinogen is a B beta-chain variant with faster than normal fibrin monomer polymerization. Fibrinogen Oslo I acted more efficiently in ADP-induced platelet aggregation, and bound to gel-filtered platelets with a higher affinity constant than did normal fibrinogen. At all concentrations more fibrinogen molecules became bound per platelet with the dysfibrinogen than with normal fibrinogen, both when the fibrinogens were tested separately or as a mixture using 125I or 131I to label the two types. At high concentrations this was probably due to ligand polymerization of the dysfibrinogen. These observations indicate that the increased cofactor function in platelet aggregation may be related to the increased affinity of the dysfibrinogen for the platelets.</p>","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"36 2","pages":"203-10"},"PeriodicalIF":0.0,"publicationDate":"1986-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0609.1986.tb00829.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14143644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Defective recloning capacity of granulocyte-macrophage colony-forming cells in chronic myeloid leukaemia.","authors":"T Olofsson, B Nilsson","doi":"10.1111/j.1600-0609.1986.tb00823.x","DOIUrl":"https://doi.org/10.1111/j.1600-0609.1986.tb00823.x","url":null,"abstract":"<p><p>We investigated the recloning capacity of normal and chronic myeloid leukaemia granulocyte-macrophage colony-forming cells (CFU-GM) after 7 d culture in methylcellulose. Normal CFU-GM were recloned with the formation of 3.52 +/- 1.12 secondary CFU-GM per primary d-7 colony. A frequency distribution of the colony-forming cells within d-7 colonies showed a heterogeneous distribution with the majority of d-7 colonies containing 1 or zero CFU-GM and a few colonies containing more than 30 CFU-GM. Separation of marrow cells by velocity sedimentation demonstrated that the recloning capacity was primarily expressed by the smallest colony-forming cells. In contrast, marrow or blood cells from 18 patients with Ph1-chromosome-positive CML in the chronic phase produced colonies with defective recloning capacity. Only 1 patient had a recloning value within the normal range; the others had a mean value of 0.35 (range 0-1.28) secondary colonies per primary d-7 colony. The recloning defect was not related to WBC or treatment, since 5 newly diagnosed patients with CML also showed defective recloning before any treatment was given, which is compatible with the defect being an inherent property of CML.</p>","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"36 2","pages":"168-75"},"PeriodicalIF":0.0,"publicationDate":"1986-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0609.1986.tb00823.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14583991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S Bellucci, S Levy-Toledano, J Maclouf, F Rendu, G Tobelem, J P Caen
{"title":"Thrombocytopenia with thrombocytopathy possibly related to abnormalities of intracellular Ca++ fluxes and followed by the development of leukaemia.","authors":"S Bellucci, S Levy-Toledano, J Maclouf, F Rendu, G Tobelem, J P Caen","doi":"10.1111/j.1600-0609.1986.tb00818.x","DOIUrl":"https://doi.org/10.1111/j.1600-0609.1986.tb00818.x","url":null,"abstract":"<p><p>A patient is described who presented a thrombocytopenia with thrombocytopathy followed by the development of a leukaemia. The disorder was characterized by decreased aggregation in the presence of ADP, and a lack of aggregation in the presence of arachidonic acid, natural endoperoxide or collagen. In parallel, 14C-serotonin release was severely decreased or nil in response to these inducers. Thrombin induced a slightly decreased aggregation and a normal 14C-serotonin release. Thromboxane B2 (T X B2) synthesis was normal after stimulation by arachidonic acid, natural endoperoxide or thrombin showing a normal arachidonate metabolism. In addition, the mepacrine test showed no significant decrease of the number of dense bodies with an average of 4.6 per platelet (versus 5.4 +/- 0.8 sd in controls). Stimulation by ionophore A 23187 failed to induce aggregation, 14C-serotonin release, or T X B2 synthesis. Furthermore, in the presence of EDTA, A 23187 did not provoke activation as reflected by 14C-serotonin release or T X B2 synthesis. Thus, in this case of thrombocytopathy, the hypothesis of abnormal intracellular Ca++ fluxes responsible for the defective platelet release phenomenon, was suggested.</p>","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"36 2","pages":"142-6"},"PeriodicalIF":0.0,"publicationDate":"1986-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0609.1986.tb00818.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14217446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cerebellar dysfunction during high dose cytosine arabinoside therapy in a case of acute myelogenous leukaemia.","authors":"S Cold","doi":"10.1111/j.1600-0609.1986.tb00822.x","DOIUrl":"https://doi.org/10.1111/j.1600-0609.1986.tb00822.x","url":null,"abstract":"<p><p>A case of persistent cerebellar toxicity following systemic high dose cytosine arabinoside (HDCA) treatment of acute myelogenous leukaemia is reported. The symptoms, which developed at a cumulative dose of 32 g/m2, subsided to some extent following discontinuation of the drug, but left signs of cerebellar dysfunction 10 months later. A review of previously published reports indicates that the cerebellar toxicity of HDCA, which has been ascribed to loss of Purkinje cells, usually occurs when the accumulated dose exceeds 36 g/m2. That it may occur at even lower doses is supported by the present case. In addition, available evidence of re-appearance or worsening of previously induced signs of toxicity following its repeated administration stresses the importance of immediate and permanent cessation of cytosine arabinoside therapy, when signs of cerebellar dysfunction develop.</p>","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"36 2","pages":"165-7"},"PeriodicalIF":0.0,"publicationDate":"1986-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0609.1986.tb00822.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14583990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N Meyerstein, U Oppenheim, T Yirmiahu, L Hatskelson, A Dvilansky
{"title":"Erythrocyte involvement in chronic lymphocytic leukaemia.","authors":"N Meyerstein, U Oppenheim, T Yirmiahu, L Hatskelson, A Dvilansky","doi":"10.1111/j.1600-0609.1986.tb00817.x","DOIUrl":"https://doi.org/10.1111/j.1600-0609.1986.tb00817.x","url":null,"abstract":"<p><p>Chronic lymphocytic leukaemia (CLL) is a lymphoproliferative disorder which sometimes also affects the erythrocytes. In this study we investigated erythrocyte involvement in 23 CLL patients. We found increased erythrocyte osmotic fragility in 15 CLL patients, but this finding was not accompanied by increased permeability to acidified glycerol (decreased AGLT50). Only those CLL patient who had positive direct antiglobulin test (DAT) had significantly decreased AGLT50. AGLT cannot serve as a predictor test for the future development of autoimmune haemolytic anaemia in CLL patients. ATP content was unaffected by the lymphoproliferative disease, but whole blood filterability was markedly decreased in CLL patients. Our study supports the hypothesis that erythrocytes are indeed affected by the lymphoproliferative disorder, even in the absence of overt autoimmune haemolytic processes.</p>","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"36 2","pages":"138-41"},"PeriodicalIF":0.0,"publicationDate":"1986-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0609.1986.tb00817.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14824061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P C Huijgens, C A van den Berg, L M Imandt, A Miltenburg, M M Langenhuijsen
{"title":"Aspirin does not inhibit human megakaryocyte thromboxane synthesis in vivo.","authors":"P C Huijgens, C A van den Berg, L M Imandt, A Miltenburg, M M Langenhuijsen","doi":"10.1111/j.1600-0609.1986.tb02656.x","DOIUrl":"https://doi.org/10.1111/j.1600-0609.1986.tb02656.x","url":null,"abstract":"<p><p>Cyclooxygenase activity in human platelets reappears after ingestion of aspirin as a function of the platelet production rate. In different studies the activity reappeared without delay or with an interval of at least 48 h after stopping the drug. Because inhibition of megakaryocyte cyclooxygenase is the sole likely explanation for a delay we determined thromboxane B2 in human megakaryocytes obtained under local anaesthesia. We found that aspirin does not inhibit human megakaryocytes in vivo but does so in vitro. Therefore, it does not seem likely that there is actually a delay in platelet cyclooxygenase resurgence after aspirin intake. In order to suppress platelet cyclooxygenase constantly, aspirin should be given once or twice a day and not once or twice a week.</p>","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"36 1","pages":"92-5"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0609.1986.tb02656.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14213576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Double gammopathies: incidence and clinical course of 20 patients.","authors":"T Nilsson, B Norberg, O Rudolphi, L Jacobsson","doi":"10.1111/j.1600-0609.1986.tb02658.x","DOIUrl":"https://doi.org/10.1111/j.1600-0609.1986.tb02658.x","url":null,"abstract":"<p><p>The presence of 2 M-components in single patients (double gammopathy) was studied retrospectively by means of the electrophoresis files in Umeå (serving a population of approximately 150 000 inhabitants). During the 11-year period 1974-1984, 109 000 electrophoreses were performed, among which 1034 patients with gammopathy were found. Of these, 20 (2%) had double gammopathy; 3 were associated with lymphoma, 7 with myelomatosis, 9 with monoclonal gammopathy of undetermined significance (MGUS), and 1 with lupus erythematosus disseminatus. It is suggested that one subgroup of double gammopathy (6 patients) had a neoplastic cell clone combined with a normally regulated cell line, the latter marked by a transient M-component. Another subgroup (14 patients) seems to have 2 coexisting different neoplastic cell lines, with persistent double gammopathy.</p>","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"36 1","pages":"103-6"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0609.1986.tb02658.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14213573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}