Scandinavian Journal of Clinical & Laboratory Investigation最新文献_第3页

Acid-base parameters in venous blood - agreement between values from safePICO syringes and lithium-heparin vacutainer tubes. 静脉血中的酸碱参数--安全PICO注射器和锂肝素空采样管的数值之间的一致性。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2024-11-01 Epub Date: 2024-12-09 DOI: 10.1080/00365513.2024.2420317

Linda Østervig Henriksen, Tina Windfeld, Willemijn Jansje Comuth, Anna-Marie Bloch Münster

{"title":"Acid-base parameters in venous blood - agreement between values from safePICO syringes and lithium-heparin vacutainer tubes.","authors":"Linda Østervig Henriksen, Tina Windfeld, Willemijn Jansje Comuth, Anna-Marie Bloch Münster","doi":"10.1080/00365513.2024.2420317","DOIUrl":"10.1080/00365513.2024.2420317","url":null,"abstract":"Venous blood is considered an acceptable alternative to arterial blood for assessment of metabolic acid-base disorders. Also, venous sampling using lithium-heparin (Li-Hep) tubes is advantageous to arterial sampling using PICO syringes, the risk of complications being lower. Usage of partly filled tubes without firm knowledge about the clinical consequences is, however, a pre-analytic consideration. The study evaluated primary acid-base parameters (pH, standardized hydrogen carbonate (HCO3), standardized base excess (SBE), lactate) and co-determined parameters in venous blood stored at room temperature up to 60 min in Li-Hep tubes vs. venous blood in PICO syringes analyzed immediately. Also, 50% filled tubes stored up to 30 min were compared to filled tubes analyzed immediately. Significant differences were generally observed. Stability was parameter and time dependent (filled tubes: 30 min: pH, (preferably 15 min for optimal stability), SBE, potassium and lactate, 60 min: HCO3, hemoglobin, methemoglobin (MetHb), carbon monoxide hemoglobin (COHb), sodium, chloride, glucose and creatinine; 50% filled tubes: 15 min: lactate, 30 min: HCO3, hemoglobin, MetHb, COHb, potassium, sodium, chloride, glucose and creatinine). In conclusion, storage in filled Li-Hep tubes for 30 min generates comparable results to blood in PICO syringes for all parameters, except pCO2, pO2 and sO2. Storage in 50% filled Li-Hep tubes is not acceptable for pH, pCO2, pO2, sO2 and SBE, and lactate is only stable for 15 min.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"459-469"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of direct oral anticoagulants on ROTEM® variables; a sample size-calculated experimental study. 直接口服抗凝剂对 ROTEM® 变量的影响；样本大小计算实验研究。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2024-11-01 Epub Date: 2024-12-14 DOI: 10.1080/00365513.2024.2439398

Lotta Sunnersjö, Lukas Lindquist, Ulf Schött, Andreas Hillarp, Johan Undén, Thomas Kander

{"title":"Impact of direct oral anticoagulants on ROTEM® variables; a sample size-calculated experimental study.","authors":"Lotta Sunnersjö, Lukas Lindquist, Ulf Schött, Andreas Hillarp, Johan Undén, Thomas Kander","doi":"10.1080/00365513.2024.2439398","DOIUrl":"10.1080/00365513.2024.2439398","url":null,"abstract":"Direct oral anticoagulants (DOAC) are increasingly common, with bleeding events associated with elevated plasma concentrations. Rotational thromboelastometry (ROTEM), a point-of-care tool for assessing secondary hemostasis, has demonstrated a correlation with increasing concentrations of DOAC. However, previous studies have only partially explored this relationship. The primary aim in the current study was to investigate the impact of increasing rivaroxaban concentrations on clotting time (CT) in the EXTEM assay. The secondary aims were to investigate the impact of increasing rivaroxaban concentrations on clot formation time (CFT) and α-angle (AA) and to investigate the impact of increasing concentrations of dabigatran and apixaban on CT, CFT and AA. Blood from 12 healthy volunteers was spiked to anticipated concentrations of rivaroxaban, dabigatran and apixaban at 0, 50, 100, 200, 500 and 1000 µg/L each. Blood with these varying concentrations was analyzed in four different ROTEM assays and CT, CFT and AA were extracted. CT increased linearly with increasing concentrations of all three DOACs. Rivaroxaban and dabigatran spiked blood showed an increase in CT-EXTEM for the 200-1000µg/L concentrations, compared to baseline, and apixaban for the 500-1000 µg/L concentrations. CFT and AA were affected only in supratherapeutic concentrations for all tested DOACs and primarily in the INTEM assay. Among the tested DOACs, apixaban demonstrated the least impact on CT across all assays. In conclusion, ROTEM-derived CT measurements can serve as surrogate markers for DOAC concentrations.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"540-546"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of protein expression profiles with genotypes in the diagnosis of Inborn Errors of Immunity. 蛋白表达谱与基因型在先天性免疫错误诊断中的比较分析。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2024-11-01 Epub Date: 2024-12-20 DOI: 10.1080/00365513.2024.2439401

Sevil Oskay Halacli, Deniz Cagdas, Saliha Esenboga, Dilan Inan, Ismail Yaz, Begum Cicek, Hacer Neslihan Bildik, Ilhan Tezcan

{"title":"Comparative analysis of protein expression profiles with genotypes in the diagnosis of Inborn Errors of Immunity.","authors":"Sevil Oskay Halacli, Deniz Cagdas, Saliha Esenboga, Dilan Inan, Ismail Yaz, Begum Cicek, Hacer Neslihan Bildik, Ilhan Tezcan","doi":"10.1080/00365513.2024.2439401","DOIUrl":"10.1080/00365513.2024.2439401","url":null,"abstract":"Background: Inborn Errors of Immunity (IEIs) are genetic diseases resulting from harmful genetic variations that hinder the proper functioning of the immune system. The broad range of IEIs involves multiple systems, presenting characteristics similar to allergies, autoimmune or inflammatory diseases, and malignancies. Given this complexity, there is an urgent need for a precise multi-parametric molecular diagnostic approach.Objective: In this work, we demonstrated the effectiveness of accurate diagnosis by flow cytometry in patients with IEI by comparing genotype analysis with the expression levels of particular proteins and signaling activities.Methods: We examined the expression levels or signaling activities of 28 cell surface and intracellular proteins using flow cytometry in a cohort of 352 patients and 189 healthy controls, in conjunction with genotype analysis for comparison. Results: We identified alterations in protein expression in 60 individuals, among them, 55 exhibited the presence of an underlying pathogenic mutation. Complete loss of protein expression was observed in seven patients, constituting 2% of the total, while reduced protein expression was noted in 35 patients (9%). Notably, despite mutations in the relevant genes, protein expression levels were normal in five patients (2%), in all investigated patients. 37% of patients had elevated signaling activity, and 17% were suggestive of a particular IEI diagnosis following protein expression analysis.Conclusion: The correspondence between flow cytometry-based protein analyses and genotype facilitates a prompt diagnosis, providing patients with swift access to therapeutic options.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"547-556"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hb Tacoma by seven HbA1c methods - one with significant interference. 用七种 HbA1c 方法检测血红蛋白塔科马--其中一种干扰很大。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2024-11-01 Epub Date: 2024-10-27 DOI: 10.1080/00365513.2024.2417383

Anni Mäenpää, Moona Kangastie, Päivikki Kangastupa

{"title":"Hb Tacoma by seven HbA1c methods - one with significant interference.","authors":"Anni Mäenpää, Moona Kangastie, Päivikki Kangastupa","doi":"10.1080/00365513.2024.2417383","DOIUrl":"10.1080/00365513.2024.2417383","url":null,"abstract":"Hemoglobin Tacoma is known to potentially interfere HbA1c assays. The variant is common in Finland with prevalence of up to 2% regionally and cases are also reported in areas that have attracted Finnish immigrants, especially in Sweden and North America. Here, we investigated the effect of Hb Tacoma on seven HbA1c methods. 20 non-variant and 20 Hb Tacoma samples were measured with Tina-quant Gen. 3 (immunoassay, considered as reference) and the following point of care instruments: Afinion 2, HbA1c 501 (both utilizing boronate affinity), QuikRead go, cobas b 101, DCA Atellica, and Standard F (all immunoassays). Repeatability was also assessed by measuring both non-variant and Hb Tacoma samples five times each at two different levels. For non-variant samples, the mean relative bias with all methods was < ±4%, whereas for Hb Tacoma samples Standard F had 38% mean relative bias. In absolute bias, the difference was 17 mmol/mol on average and constant through the measured range. For other methods the mean relative bias for Hb Tacoma samples was < ±6%. The repeatability with all methods was similar for non-variant and Hb Tacoma samples and at highest 4.1% (mean CV% of two levels). The observed interference by Standard F is likely due to two-antibody assay design as Hb Tacoma has been shown to result in conformational change. This interference is clinically significant and highlight the need for better controlling and better understanding hemoglobin variants in HbA1c testing.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"447-451"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142507042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analytical interference on measurement of immunoglobulins in monoclonal gammopathy. 单克隆抗体病中免疫球蛋白测量的分析干扰。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2024-11-01 Epub Date: 2024-11-16 DOI: 10.1080/00365513.2024.2429081

Anders Oest, Maja Boe, Marianne Christina Harbo Mosgaard, Jeanette Elgaard, Simon Lykkeboe, Paw Jensen, Henrik Gregersen, Stine Linding Andersen, Aase Handberg, Stine Krogh Venø

{"title":"Analytical interference on measurement of immunoglobulins in monoclonal gammopathy.","authors":"Anders Oest, Maja Boe, Marianne Christina Harbo Mosgaard, Jeanette Elgaard, Simon Lykkeboe, Paw Jensen, Henrik Gregersen, Stine Linding Andersen, Aase Handberg, Stine Krogh Venø","doi":"10.1080/00365513.2024.2429081","DOIUrl":"10.1080/00365513.2024.2429081","url":null,"abstract":"Monoclonal gammopathy has been reported to interfere with several laboratory measurement results. We investigated potential interference in monoclonal gammopathy on immunoglobulin A (IgA), immunoglobulin G (IgG), and immunoglobulin M (IgM) concentrations using three different routine chemistry instruments: Alinity (Abbott Laboratories), Cobas 8000 (Roche Diagnostics) and Optilite (Binding Site) at the Department of Clinical Biochemistry, Aalborg University Hospital, Denmark. Blood samples collected from 216 patients with known monoclonal gammopathy were analyzed on Alinity, Cobas and Optilite. Diagnoses were ascertained from clinical records. Scatter plots with Passing-Bablok regression were used to investigate associations between concentrations of IgA, IgG and IgM measured with each of the different instruments. Furthermore, comparison of IgG analyses on Alinity, Optilite and Cobas according to monoclonal IgM concentration was explored with Bland-Altman plots. A total of 81 patients were identified with IgM type monoclonal gammopathy and 8 of these patients (10%) had analytical interference as reflected by more than 25% decrease in the level of IgG measured on Alinity as compared to Optilite and Cobas. All blood samples with interference on IgG measurement using Alinity were from patients with lymphoplasmacytic lymphoma/Waldenströms macroglobulinemia and all of them had monoclonal IgM concentrations above 10 g/L. This study identified a source of interference from IgM monoclonal gammopathy on IgG measurement performed using Alinity. The interferences caused falsely low results of IgG which is important to recognize to ensure proper patient management.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"502-509"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Within- and between-subject biological variation data for whole blood HbA_1c from 38 apparently healthy Turkish subjects. 38名明显健康的土耳其受试者全血HbA1c的受试者内部和受试者之间生物学变异数据。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2024-11-01 Epub Date: 2024-12-16 DOI: 10.1080/00365513.2024.2439394

Murat Keleş, Gönül Ünver Şeker

{"title":"Within- and between-subject biological variation data for whole blood HbA1c from 38 apparently healthy Turkish subjects.","authors":"Murat Keleş, Gönül Ünver Şeker","doi":"10.1080/00365513.2024.2439394","DOIUrl":"10.1080/00365513.2024.2439394","url":null,"abstract":"HbA1c plays an important role in the diagnosis and treatment of diabetes and is a valuable biomarker for evaluating glycemic control and predicting the risk of vascular complications. The study aimed to determine the biological variation (BV) for HbA1c and thereby contribute to analytical performance specifications, reference change values, and index of individuality. Fasting venous whole blood samples were collected from 38 presumably healthy subjects (20 females, 18 males) once a week for ten weeks, and analyzed in duplicate using the Roche Cobas c501 analyzer. BioVar, an online R-based biological variation analysis tool, was used for the statistical analysis. BV values were obtained by analysis of variance (ANOVA) after outlier detection, normality tests, steady-state, and homogeneity checks. The within-subject biological variation for HbA1c was 2.9%, and the between-subject biological variation was 7.9%. The index of the individuality of HbA1c was 0.37. Derived desirable analytical goals for imprecision, bias, total allowable error, and maximum expanded allowable measurement uncertainty were 1.4%, 1.8%, 4.2%, and 2.9% respectively. The reference change value is more appropriate for interpreting HbA1c results than a population-based reference interval.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"535-539"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of monoclonal proteins in patients with isolated hypogammaglobulinemia on serum protein electrophoresis. 血清蛋白电泳中孤立性低丙种球蛋白血症患者的单克隆蛋白患病率。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2024-11-01 Epub Date: 2024-11-14 DOI: 10.1080/00365513.2024.2429090

Joris Godelaine, Toon Schiemsky, Ben Persy, Joris Penders

{"title":"Prevalence of monoclonal proteins in patients with isolated hypogammaglobulinemia on serum protein electrophoresis.","authors":"Joris Godelaine, Toon Schiemsky, Ben Persy, Joris Penders","doi":"10.1080/00365513.2024.2429090","DOIUrl":"10.1080/00365513.2024.2429090","url":null,"abstract":"Isolated hypogammaglobulinemia (IH) is an electrophoretic pattern that can be encountered on serum protein electrophoresis (SPEP) and is defined as a decreased but morphologically normal γ-globulin fraction with normal α- and β-globulin fractions. SPEP is mainly used to detect monoclonal proteins which are usually observed as additional peaks in the electropherogram. However, they may also be more discretely present in a significant proportion of patients presenting with IH. Therefore, we aimed to evaluate i) via both retrospective and prospective analysis to what extent paraproteins as identified by immunofixation are present in patients demonstrating IH on SPEP and ii) whether other parameters may predict their presence in IH-patients. For this purpose, we first reviewed historic SPEP- and immunofixation results in our tertiary hospital and determined paraprotein prevalence in this retrospective cohort. This analysis showed immunofixation was requested in only 519/3938 (13.2%) historic IH-results with 52/519 (10%) patients demonstrating paraproteins. Next, various laboratory parameters were compared between paraprotein-positive and -negative patients and subjected to logistic regression models but regrettably, no parameter could be retained as promising predictor of paraproteins. Lastly, to confirm paraprotein prevalence seen in the historical query, we conducted a six-month prospective analysis during which immunofixation was requested more frequently in IH-cases during routine diagnostics and which showed paraproteins to be present in 20/83 (24.1%) of IH-patients. Hence, as up to 24% of patients with IH may harbour paraproteins, one should consider performing follow-up analyses (e.g. immunofixation, urine electrophoresis and/or free light chain analysis) for all IH-cases identified via SPEP.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"510-514"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reference intervals for 23 common biochemical parameters during pregnancy and the first six postpartum months. 孕期和产后头 6 个月 23 项常见生化指标的参考区间。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2024-11-01 Epub Date: 2024-11-16 DOI: 10.1080/00365513.2024.2406006

Paul Kjetel Soldal Lillemoen, Kristin Holstad, Anne-Lise Bjørke-Monsen

{"title":"Reference intervals for 23 common biochemical parameters during pregnancy and the first six postpartum months.","authors":"Paul Kjetel Soldal Lillemoen, Kristin Holstad, Anne-Lise Bjørke-Monsen","doi":"10.1080/00365513.2024.2406006","DOIUrl":"10.1080/00365513.2024.2406006","url":null,"abstract":"The concentration of many biochemical parameters changes significantly during pregnancy and the postpartum period, causing a need for specific reference values for biochemical parameters in these life periods. We have collected blood samples in pregnancy week 18, 28 and 36 and six weeks, four months and six months postpartum from 139 healthy women. Blood samples from 59 healthy never-pregnant women were included as controls. Serum samples were analyzed for 23 common biochemical parameters and reference intervals for pregnancy and postpartum periods were computed with the non-parametric method in accordance with recommendations from IFCC and CLSI. The dynamics of different biochemical parameters during and after pregnancy vary considerably between parameters. Some analytes like serum amylase and HDL cholesterol do not differ from never-pregnant values. Of the 23 parameters, serum alkaline phosphatase, chloride, gamma-glutamyl transferase and sodium and urea which were still significantly changed at six months postpartum compared to never-pregnant women. The physiological changes related to pregnancy and lactation have profound effects on biochemical parameters, denoting the use of specific reference intervals for both pregnancy and the postpartum period.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"429-440"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The course of plasma alpha-1-microglobulin and haemolysis during cardiac surgery and the relationship to acute kidney injury, a pilot study. 心脏手术期间血浆α -1微球蛋白和溶血的过程及其与急性肾损伤的关系，一项初步研究。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2024-11-01 Epub Date: 2024-12-27 DOI: 10.1080/00365513.2024.2442022

Carsten Metzsch, Oscar Lindahl, Martina Klarén, Edgars Grins, Annika Ekroth, Stefan R Hansson, Bo Åkerström, Alain Dardashti

{"title":"The course of plasma alpha-1-microglobulin and haemolysis during cardiac surgery and the relationship to acute kidney injury, a pilot study.","authors":"Carsten Metzsch, Oscar Lindahl, Martina Klarén, Edgars Grins, Annika Ekroth, Stefan R Hansson, Bo Åkerström, Alain Dardashti","doi":"10.1080/00365513.2024.2442022","DOIUrl":"10.1080/00365513.2024.2442022","url":null,"abstract":"Haemolysis occurring during cardiac surgery with cardiopulmonary bypass (CPB) is assumed to be a risk factor for postoperative acute kidney injury (AKI). Plasma alpha-1 microglobulin (A1M) may have a protective role as haem scavenger. The aim of this study was to evaluate the association between AKI and the degree of haemolysis and the course of A1M concentrations during cardiac surgery, respectively. We analysed plasma concentrations of free haemoglobin (pfHb) and A1M in 25 patients undergoing cardiac surgery: before CPB; during CPB in 15 min intervals; after CPB; and at four additional time points until 24 h after surgery. Markers of kidney function were followed until 4 days after surgery. Detection of AKI was based on the KDIGO (Kidney Disease, Improving Global Outcome) criteria. The plasma concentration of free haemoglobin during CPB was found to be significantly higher in patients with postoperative AKI at 60 min after start of CPB [mean 1379 µg/mL (95% CI: 1037-1721)]; compared to [820 µg/mL (622-1018)]; p = 0.034, in patients without AKI, and at one hour post-CPB [2600 µg/mL (969-4230)] vs [1037 µg/mL (722-1353)]; p = 0.044]. There was no significant difference found for pA1M levels between the groups with and without postoperative AKI development. Haemolysis during cardiac surgery with CPB increases the risk of postoperative AKI. Levels of pA1M did not differ for patients who developed postoperative AKI compared with those who did not. The data did not allow conclusions regarding the hypothesis that pA1M has a reno-protective effect.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"557-563"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of serum human epididymis protein 4 in children with chronic liver diseases. 慢性肝病患儿血清人附睾蛋白4的测定。

IF 1.3 4区医学

Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2024-11-01 Epub Date: 2024-12-05 DOI: 10.1080/00365513.2024.2437612

Nashwa Farouk Mohamed, Ola Galal Ali Behairy, Manal Sadek El Defrawy, Samar Ahmed Mohamed Elsheraki, Rana Atef Khashaba

{"title":"Evaluation of serum human epididymis protein 4 in children with chronic liver diseases.","authors":"Nashwa Farouk Mohamed, Ola Galal Ali Behairy, Manal Sadek El Defrawy, Samar Ahmed Mohamed Elsheraki, Rana Atef Khashaba","doi":"10.1080/00365513.2024.2437612","DOIUrl":"10.1080/00365513.2024.2437612","url":null,"abstract":"The aim of this study was to evaluate the role of serum human epididymis protein 4 (HE4) as a non-invasive biomarker for the diagnosis of liver fibrosis in children with chronic liver diseases (CLD). This case-control study was conducted at Benha University Hospital, Egypt, involving 60 children with CLD and 60 healthy children as a control group. HE4 levels were measured by ELISA and compared with liver biopsy results. The CLD group had significant higher HE4 (median: 110.7, IQR: 96.7-120.4 pmol/L) compared to control group (median 42.07, IQR: 41.67-43.05 pmol/L), p < .001. HE4 levels increased significantly with the degree of fibrosis and histological activity index. At a cutoff point >48.3 pmol/L, HE4 diagnosed cases with mild fibrosis with a sensitivity of 95% and specificity of 91.3%. At a cutoff point >144.3 pmol/L, HE4 diagnosed cases with severe fibrosis with a sensitivity of 98% and specificity of 93.1%. Serum HE4 is a potential non-invasive marker for detecting liver fibrosis and its severity in children with CLD.","PeriodicalId":21474,"journal":{"name":"Scandinavian Journal of Clinical & Laboratory Investigation","volume":" ","pages":"515-520"},"PeriodicalIF":1.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0