M P Portillo, A M Rocandio, M A Garcia-Calonge, E Diaz, E Campo, C Martinez-Blazquez, J Errasti, A S del Barrio
{"title":"Lipolytic effects of beta1, beta2 and beta3-adrenergic agonists in isolated human fat cells from omental and retroperitoneal adipose tissues.","authors":"M P Portillo, A M Rocandio, M A Garcia-Calonge, E Diaz, E Campo, C Martinez-Blazquez, J Errasti, A S del Barrio","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The presence of beta1- and beta2-adrenoceptors has been clearly established in human fat cells. There is some controversy about the presence and function of beta3-adrenoceptors. It is well established that there are marked regional variations in catecholamine-induced lipolysis. In this work the possibility that a beta3-adrenoceptor plays a significant role in the control of lipid mobilization is studied and also its importance in comparison to beta1- and beta2-adrenoceptors in isolated human fat cells, is evaluated, by measuring the in vitro lipolysis induced by dobutamine, salbutamol, metaproterenol, BRL 37344 and CGP 12177A. Human adipocytes from omental and retroperitoneal fat deposits exhibited an \"atypical\" beta-adrenergic response but, given the small lipolytic effect initiated by BRL 37344 and CGP 12177A, they are probably poorly equipped in functional beta3-adrenoceptors.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"51 4","pages":"193-200"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19872561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D Montero, L L Tort, M S Izquierdo, J Socorro, J M Vergara, L Robaina, H Fernandez-Palacios
{"title":"Hematological recovery in Sparus aurata after bleeding. A time course study.","authors":"D Montero, L L Tort, M S Izquierdo, J Socorro, J M Vergara, L Robaina, H Fernandez-Palacios","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To determine the hematological recovery after blood loss in gilthead sea bream juveniles, an experimental anemia was induced by caudal blood extraction. Seventy fish were used for experimental hemorrhage and another 35 were used as controls. Blood samples were taken after 0.5, 1, 2, 4, 8, 16 and 32 days in both control and anemic groups. After bleeding, besides a reduction in the direct hematic constants, a peak in the mean cell volume value, together with a decrease in mean cell hemoglobin concentration after bleeding, suggested erythrocyte swelling. The increase in MCH suggested the occurrence of hyperchromic erythrocytes. Recovery of RBC number started between 2 and 4 days after bleeding and seemed to be related to erythroblast release to circulation. Total recovery was completed after 8 days of bleeding. The recovery patterns for hematological parameters of sea bream are discussed in relation to applied work on this species.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"51 4","pages":"219-26"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19872565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Changes in skeletal metabolism after the administration of demineralized bone proteins and fluoride.","authors":"M Elorriaga, F Lecanda, J A Martínez, J Larralde","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"51 3","pages":"163-4"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19586292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J L Rey de Viñas, M González-Fernández, M C Sandoval
{"title":"Liver DNA and RNA metabolism in rats fed diets lacking methionine plus cysteine with or without restricted energy.","authors":"J L Rey de Viñas, M González-Fernández, M C Sandoval","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Ponderal parameters, soluble protein content and nucleic acid (DNA and RNA) metabolism were studied in liver of growing male Wistar rats fed with different diets: control diet (Control group) containing 10% protein (casein 9.8% plus DL-methionine 0.2%); diet 2 (Group 2) containing 10% protein, lacking Met plus Cys; and diet 3 (Group 3), containing 20% protein, lacking Met plus Cys and with 50% of energy restriction (restricted food intake by experimental design). Diets 2 and 3 were compared with the control diet to know the effects produced by the lack of Met plus Cys and the energy restriction, for an experimental period of 14 days, the animals being slaughtered on the 4th, 8th and 14th days. Food intake, body and liver weights, relative liver weight, cellular size and RNA content per organ and per mg protein decreased in groups 2 and 3, group 3 being affected more than group 2. Diet 2 produced a decrease in DNA content, due to lack of Met+Cys. Acid DNAse activity per organ diminished in group 2 on days 8 and 14, and in group 3 on the 8th day. RNA/DNA ratio diminished in group 2 and 3 due to a proportional RNA reduction with respect to the DNA content. Acid RNAse activity per organ diminished in group 2 on the 8th and 14th days, RNAse per mg of protein increased in group 3 at the end of the treatment, therefore the RNA content decreased. The content of DNA in liver is lower than RNA content in rats fed diet 2 and the opposite occurs with diet 3.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"51 3","pages":"129-38"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19586288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V M Pinto-Plata, J C Pozo-Parilli, A Baum-Agay, C Curiel, R Sánchez de León
{"title":"Effect of blood pH on pulmonary artery pressure, left atrial pressure and fluid filtration rate in isolated rabbit lung.","authors":"V M Pinto-Plata, J C Pozo-Parilli, A Baum-Agay, C Curiel, R Sánchez de León","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To determine the effects of pH changes on Pulmonary Artery Pressure (PAP), 18 isolated rabbit lung preparations, perfused with autologous blood mixture and constant PaCO2 have been studied. Each preparation was studied under 3 conditions: Baseline: 30 minutes equilibration period. Acidosis: pH was decreased by 0.2 N HCl infusion, the ventilatory rate was changed and different CO2 mixtures were used to maintain the PCO2 within the initial parameters. Compensated Acidosis (CA): pH was returned to normal values by 0.7 N NaHCO3 infusion maintaining PCO2 in its initial values. The decrease in pH (acidosis) from 7.36 +/- 0.05 to 7.18 +/- 0.06 at constant PCO2, generated a significant increase in PAP (13.6 +/- 3.2 cm H2O to 18.8 +/- 5.2 cm H2O, p < 0.01). The pH increase (CA) from 7.18 +/- 0.06 to 7.40 +/- 0.09 caused the PAP to decrease (18.8 +/- 5.2 cm H2O to 15.9 +/- 4.2 cm H2O); the fluid filtration rate remained unchanged during the whole experiment. It is concluded that blood pH changes at constant PCO2 result in significant changes of PAP. Acidemia produces pulmonary vasoconstriction, which may be a contributing factor in the genesis of pulmonary hypertension in clinical conditions with increased hydrogen ion concentration [H+].</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"51 3","pages":"117-23"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19586286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of zinc on D-galactose and L-phenylalanine uptake in rat intestine in vitro.","authors":"A Lugea, A Barber, F Ponz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of zinc on galactose and phenylalanine uptake was studied using rat everted jejunal rings. The rings were incubated for 2 min in oxygenated Krebs-Ringer-Tris (KRT) solution. Galactose and phenylalanine uptake was reduced by zinc in a dose-dependent manner, but not in a time-dependent way. One mM Zn2+ but not 0.5 mM Zn2+ inhibited galactose transport without modifying sugar diffusion. Na(+)-dependent phenylalanine transport was reduced by 0.5 mM and 1 mM Zn2+. However, the metal did not change phenylalanine diffusion obtained in the presence of 40 mM L-methionine or Na(2+)-independent phenylalanine transport. Therefore, zinc seems to interact only with the sodium-galactose or sodium-phenylalanine cotransporters. Zinc inhibited sugar and amino acid transport in a non-competitive way, without a significant change in the affinity of the transporters for their substrates and with a Vmax decrease. The inhibitory effect of Zn2+ on galactose and phenylalanine uptake was reversed by washing intestinal rings for 5 min with KRT solution. These results suggest that zinc might exert its inhibitory action by a weak binding to chemical groups related with sodium-substrate cotransporters and located in the luminal membrane of the enterocytes.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"51 3","pages":"139-46"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19586289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Analysis of the interactions between growth hormone and metaproterenol on lipid mobilization.","authors":"M P Portillo, A S del Barrio, J A Martínez","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The possible modification of the in vitro lipolytic action of rat growth hormone (rGH) or a mixed beta-adrenergic agonist (metaproterenol) on rat adipose tissue after a previous acute treatment with these compounds was assessed by measuring glycerol release from adipocytes. The involvement of adenosine deaminase (ADA) and dexamethasone, was also considered. The results showed that the previous acute treatment with rGH or the beta-adrenergic agonist did not alter the in vitro rGH or metaproterenol lipolytic response. The presence of ADA at a non-lipolytic concentration per se (0.02 U/ml) potentiated the lipolytic response of both compounds. Also, the addition of non-lipolytic concentrations of dexamethasone (0.5 microM) or beta-adrenergic agonist (10(-7)M) to the incubation medium potentiated the rGH lipolytic response, while the metaproterenol-induced glycerol release was not affected by the simultaneous addition of a rGH concentration (2 x 10(-7) M) which had no lipolytic effect per se.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"51 3","pages":"147-54"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19586290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sublethal effect of mercury and lead on monoamine oxidase in different regions of the brain in three freshwater teleosts.","authors":"S A Shaffi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Effect of mercury and lead at 24 and 48 h was investigated on monoamine oxidase (MAO) activity in different regions of the brain (telencephalon, cerebellum, diencephalon and medulla oblongata) in Labeo rohita, Ham., Clarias batrachus, L. and Channa punctatus, Bloch. Highest rise in MAO activity was recorded in telencephalon with mercury followed by lead. Maximum variations in the level of MAO activity in different regions of the brain were recorded at 24 h exposure. The observed alterations were discussed in relation to different parameters.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"51 3","pages":"125-8"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19586287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Electrolyte and water contents in organs and tissues of rats during and after exposure to prolonged restriction of motor activity.","authors":"Y G Zorbas, A L Ivanov, Y F Federenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Sodium and potassium concentrations in organs and tissues of rats have been determined during hypokinesia (HK) and posthypokinesia (post-HK) periods. The study was performed on 180 male Wistar rats with an initial body weight of 370 to 390 g. They were divided into control and experimental groups: the 1st group of rats was placed under ordinary vivarium conditions (control) and the 2nd group was subjected to 90 days of HK (hypokinetic animals). The animals were kept in small individual wooden cages which restricted their movements in all directions without hindering feed and water intake. On the 30th, 60th and 90th day of the HK period and on the 5th, 10th and 15th day of the post-HK period animals were decapitated, and potassium and sodium concentrations were measured in the myocardium, skeletal muscles, erythrocytes and plasma. Fluid content was also determined in the myocardium and skeletal muscles. During both the HK period and initial stages of the post-HK period the concentration of sodium and potassium in the myocardium, skeletal muscle, plasma and erythrocytes as well as water content in the myocardium and skeletal muscle changed significantly.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"51 3","pages":"155-62"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19586291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"One day estrous cycle shortening induced by antiprogestagen RU486 administration in proestrus to 4-day cyclic rats.","authors":"M Tébar, A Ruiz, J E Sánchez-Criado","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Administration of 4 mg of the antiprogestagen RU486 to 4-day cyclic rats in proestrus, which blocks proestrous and diestrous progesterone actions, induced a one day shortening of the ovarian cycle and a reduction of the ovulation rate in the following cycle. These effects were not present when RU486 was administered in estrus or metestrus. RU486 injections either in proestrus or estrus increased the serum levels of LH and 17 beta-estradiol during metestrus. However, only rats injected with RU486 in proestrus presented a 24 hour advancement of the preovulatory surge of gonadotropins and a lack of the LH-inhibiting effect of exogenous estradiol. These results suggest that, in 4-day cyclic rats, the secretion of progesterone by the corpora lutea during diestrous phase retards the follicular development by lowering the serum concentrations of LH, whereas progesterone secretion by the preovulatory follicles in proestrus regulates the estrous cycle length by antagonizing the desensitization of the pituitary to the estrogen negative feedback on LH secretion.</p>","PeriodicalId":21473,"journal":{"name":"Revista espanola de fisiologia","volume":"51 2","pages":"85-91"},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19513081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}