Scanning electron microscopy最新文献_第4页

Surface ultrastructure of human megakaryocytes sorted on the basis of DNA content. 根据DNA含量分选的人巨核细胞表面超微结构。

Scanning electron microscopy Pub Date : 1986-01-01

M A Weller, J Szela, M I Barnhart, A Nakeff

引用次数: 0

The enamel ultrastructure of multituberculate mammals: a review. 多瘤哺乳动物牙釉质超微结构研究进展。

Scanning electron microscopy Pub Date : 1986-01-01

D W Krause, S J Carlson

{"title":"The enamel ultrastructure of multituberculate mammals: a review.","authors":"D W Krause, S J Carlson","doi":"","DOIUrl":"","url":null,"abstract":"The enamel ultrastructure of multituberculate mammals has been sampled extensively and studied intensively and is better known than for any other group of early mammals. The enamel of the earliest multituberculates, those of the Late Triassic-Early Jurassic suborder Haramiyoidea and the Late Jurassic-early Early Cretaceous suborder Plagiaulacoidea, is \"preprismatic.\" With only two exceptions, all Late Cretaceous and early Tertiary genera of multituberculates examined have prismatic enamel. Prisms are either small with circular (complete) boundaries or large with arc-shaped (incomplete) boundaries. There is a remarkably consistent relationship between enamel ultrastructural type and subordinal taxa in that small, circular prisms are usually found within the suborder Ptilodontoidea and large, arc-shaped prisms are usually found in the suborder Taeniolabidoidea and in six Late Cretaceous-Early Tertiary genera of indeterminate subordinal status. Research currently in progress suggests that both small, circular prisms and large, arc-shaped prisms are homologous in all multituberculates in which they occur, with one exception. Neoliotomus, a taeniolabidoid, appears to have evolved small, circular prisms independently. In addition, it appears that large, arc-shaped prisms represent the primitive condition in multituberculates with prismatic enamel, not small, circular prisms as has been proposed previously.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 4","pages":"1591-607"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14928795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracellular structure of the outer hair cell of the organ of Corti. 器官外毛细胞的胞内结构。

Scanning electron microscopy Pub Date : 1986-01-01

Y Harada, T Sakai, N Tagashira, M Suzuki

引用次数: 0

Gene and teratogen induced defects of early central nervous system development. 基因和致畸原诱导的早期中枢神经系统发育缺陷。

Scanning electron microscopy Pub Date : 1986-01-01

K S O'Shea

引用次数: 0

Integrated image and X-ray microanalysis of hepatic lysosomes in a patient with idiopathic hemosiderosis before and after treatment by phlebotomy. 特发性含铁血黄素沉着患者放血前后肝脏溶酶体的综合图像和x线微量分析。

Scanning electron microscopy Pub Date : 1986-01-01

M I Cleton, J M Roelofs, C J Blok-Van Hoek, W C De Bruijn

{"title":"Integrated image and X-ray microanalysis of hepatic lysosomes in a patient with idiopathic hemosiderosis before and after treatment by phlebotomy.","authors":"M I Cleton, J M Roelofs, C J Blok-Van Hoek, W C De Bruijn","doi":"","DOIUrl":"","url":null,"abstract":"Morphometrical and X-ray elemental information was extracted from Scanning Transmission Electron Microscopy (STEM) images of hepatic lysosomes of a patient with idiopathic hemosiderosis before and after treatment by phlebotomy. The elements of interest were iron, stored in pathological quantities in hepatic lysosomal structures and cerium, used as a capture ion after a cytochemical reaction to detect acid phosphatase activity in the lysosomal structures. Morphologically the lysosomal structures are heteromorph and the elements iron and cerium are heterogeneously distributed. With \"reduced raster\" (= reduced scanning area) analysis at 16 X 16 pixelpoints (integrating image and X-ray microanalysis), a marked difference in the area of the cross sectioned lysosomal structures before and after treatment could be demonstrated. Simultaneously the difference in the relative orientation of the elements iron and cerium before and after phlebotomy could be visualized. Chelex ion exchange beads, loaded with 11.5% w/w iron, and coembedded with the tissue blocks, were used as an internal standard. A mean iron peak to background ratio was obtained and a factor, converting ratio to absolute iron concentration, was calculated. The same calculation procedure, now per pixelpoint, was followed for the hepatic lysosomal structures. A marked difference in iron concentration in the individual lysosomal structures was observed before and after treatment by phlebotomy.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 3","pages":"999-1006"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14915813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cells from Xenopus laevis gastrulae adhere to fibronectin-sepharose beads and other lectin coated beads. 非洲爪蟾原胚细胞粘附在纤维连接蛋白-sepharose珠和其他凝集素包被珠上。

Scanning electron microscopy Pub Date : 1986-01-01

K E Johnson, M H Silver

引用次数: 0

Phase transitions of calcium oxalate trihydrate and epitaxy in the weddellite-whewellite system. 三水合草酸钙在weddellite-whewellite体系中的相变与外延。

Scanning electron microscopy Pub Date : 1986-01-01

S Deganello

引用次数: 0

Intrauterine device (IUD) associated pathology: a review of pathogenic mechanisms. 宫内节育器(IUD)相关病理:致病机制的回顾。

Scanning electron microscopy Pub Date : 1986-01-01

W A Schmidt, K L Schmidt

{"title":"Intrauterine device (IUD) associated pathology: a review of pathogenic mechanisms.","authors":"W A Schmidt, K L Schmidt","doi":"","DOIUrl":"","url":null,"abstract":"This paper summarizes our studies of IUD-related disease with those previously published by others. Our data are based upon 51 IUDs and 42 index cases of IUD-related disease demonstrating specific processes. Gross, dissecting microscope, scanning electron microscope and X-ray microanalysis examinations were made of selected IUDs and associated tissues. Tissue associated with the IUDs revealed inflammation in 59.4%, calcific material in 6.3% and no abnormality in 34.4%. IUD-associated tissue responses were accompanied by changes of the IUD; these changes involved deposition of substances upon the IUD surface and degradation of the IUD itself. Disintegration of the IUD, its string or both, has been repeatedly observed. The material deposited upon the surface of the IUD included proteins and calcium salts. The changes which involve the IUD and the host appear to be operative in the genesis of IUD-related disease. Inflammatory changes and infections are the most common IUD-related disease processes and are also the mechanisms commonly associated with the most serious complications of IUD use, reproductive failure and death. We propose that serious IUD-related disease is caused by or is a direct consequence of processes which alter the IUD and which potentiate inflammation and infection. A model amenable to testing is proposed.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 2","pages":"735-56"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14663760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The three-dimensional microstructure of the liver. A review by scanning electron microscopy. 肝脏的三维微观结构。扫描电镜技术综述。

Scanning electron microscopy Pub Date : 1986-01-01

G Macchiarelli, P M Motta

{"title":"The three-dimensional microstructure of the liver. A review by scanning electron microscopy.","authors":"G Macchiarelli, P M Motta","doi":"","DOIUrl":"","url":null,"abstract":"The improvement in scanning electron microscopy (SEM) techniques has permitted us to describe the microstructure of the liver. By SEM, the liver peritoneal surface is composed of flat mesothelial cells possessing microvilli and cilia. Hepatic sinusoids connect the portal vessels with the terminal branches of the hepatic vein (central veins). Endothelial cells of the portal space arteries are elongated and arranged longitudinally, while those of the central and portal veins are polygonal and flattened, possessing microvilli. The sinusoidal endothelial cells show both small fenestrations (sieve plates), up to 200 nm in diameter, and large ones, up to 1 micron. Within the sinusoids are seen bridging structures, covered by fenestrated endothelium, seeming to have a fibrillar core. Kupffer cells resemble macrophages, showing microvilli, blebs, lamellipodia and filopodia. Within the Space of Disse are seen the fat-storing cells, having laminar dendritic projections. The polyhedral liver cell faces the Space of Disse (vascular pole) or faces an adjacent hepatocyte (biliary pole). Vascular facets are evenly covered by microvilli. Biliary facets show a central longitudinal depression, bordered by microvilli (bile hemicanaliculi). Canaliculoductular junction and bile duct epithelia show blebs, microvilli and cilia. Up to now, fetal liver and liver pathology have been scarcely investigated by SEM: in the future, they can be successfully approached by three-dimensional studies.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 3","pages":"1019-38"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14662830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormonal regulation of capillary fenestrae in the rat adrenal cortex: quantitative studies using objective lens staging scanning electron microscopy. 大鼠肾上腺皮质毛细血管窗的激素调节:物镜分期扫描电镜定量研究。

Scanning electron microscopy Pub Date : 1986-01-01

R P Apkarian, J C Curtis

{"title":"Hormonal regulation of capillary fenestrae in the rat adrenal cortex: quantitative studies using objective lens staging scanning electron microscopy.","authors":"R P Apkarian, J C Curtis","doi":"","DOIUrl":"","url":null,"abstract":"High magnification studies of the fenestrated capillary endothelium in the zona fasciculata (ZF) of rat adrenal glands were performed using the objective lens stage of an analytical scanning electron microscope (SEM) equipped with a lanthanum hexaboride emitter (LaB6). Resolution of surface substructure of the luminal membrane obtained with specimens decorated with gold/palladium (Au/Pd) was compared with that observed in others sputter coated with tantalum (Ta). High magnification (50,000x) of the fenestrated endothelium demonstrates that tantalum coating of the cryofractured adrenals improves the substructural detail compared to that seen in Au/Pd decorated specimens. The procedures used in specimen preparation, metal deposition and secondary electron imaging (SEI) are described. Quality imaging achieved using the objective lens stage is a result of the elimination of the SE-III component derived from backscattered electrons. Rat adrenals exhibited uniformly patent capillaries. High magnification micrographs of capillary walls were randomly recorded in two morphometric studies of the fenestral content of capillaries in the rat adrenal cortex. Adrenocorticotropic hormone (ACTH), when administered to rats following dexamethasone (DEX) treatment, significantly reduced the fenestrae/micron 2 of endothelial surface and increased the mean size of fenestrae. After hypophysectomy, the number of fenestrae/micron 2 declined over 48 h; within 2 h after ACTH was given to rats hypophysectomized 48 hours earlier, the fenestrae/micron 2 had increased two-fold. These studies indicate that ACTH plays an important role in modulating fenestral content of the capillary endothelium in the adrenal cortex.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 4","pages":"1381-93"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14160553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0