Scanning electron microscopy最新文献_第3页

The developmental features of marrow stroma in ectopic bone marrow implants. 异位骨髓移植骨髓基质的发育特征。

Scanning electron microscopy Pub Date : 1986-01-01

K Harjes, B Collier, M Tavassoli

引用次数: 0

Proton microprobe analysis in biology. 生物学中的质子微探针分析。

Scanning electron microscopy Pub Date : 1986-01-01

K G Malmqvist

引用次数: 0

Immunoarchitecture of the regenerating rat spleen: effects of partial splenectomy and heterotopic autotransplantation. 再生大鼠脾的免疫结构:脾部分切除和异位自体移植的影响。

Scanning electron microscopy Pub Date : 1986-01-01

M C Dugan, T M Grogan, M H Witte, C Rangel, L Richter, C L Witte, D B Van Wyck

{"title":"Immunoarchitecture of the regenerating rat spleen: effects of partial splenectomy and heterotopic autotransplantation.","authors":"M C Dugan, T M Grogan, M H Witte, C Rangel, L Richter, C L Witte, D B Van Wyck","doi":"","DOIUrl":"","url":null,"abstract":"To investigate the microstructure of in situ (eutopic) and autotransplanted (ectopic) splenic remnants, adult Sprague-Dawley rats were studied 60 days after subtotal (approximately 80%) splenectomy, total splenectomy followed by single or multiple remnant intraperitoneal autotransplantation, or sham operation. Total nucleated cell counts were determined in excised splenic remnants, and immunohistochemical staining using monoclonal antibodies to rat B- and T-cell antigens was performed in serial tissue sections. Immunoarchitecture of eutopic remnants was indistinguishable from that of intact spleens and total nucleated cell counts remained proportional to weight. In contrast, ectopic remnants showed sparsity and abnormal mixing of B and T lymphocyte subpopulations with widespread loss of follicles and periarteriolar lymphoid sheaths in addition to lower density and marked reduction of total nucleated cells. These findings provide immunohistologic evidence that preservation of intact vasculature is critical to splenic architecture, which may account in part for the demonstrable functional inferiority of ectopic remnants.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 2","pages":"607-13"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14663756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calcium and cystic fibrosis. 钙和囊性纤维化。

Scanning electron microscopy Pub Date : 1986-01-01

G M Roomans

{"title":"Calcium and cystic fibrosis.","authors":"G M Roomans","doi":"","DOIUrl":"","url":null,"abstract":"Cystic fibrosis (CF) is a generally lethal, congenital, genetic disease of unknown etiology. It is likely that a defective regulation of ion and water transport in exocrine glands and possibly also in other epithelial cells has a central role in the pathogenesis of this disease. Calcium has been implicated in the basic defect underlying CF because of findings of abnormally high calcium levels in some secreted fluids and some cells of CF patients. Using X-ray microanalysis, we have demonstrated elevated calcium concentrations in cultured fibroblasts and in goblet cells of the bronchial epithelium of CF patients. A factor produced by CF fibroblasts in culture can increase the calcium concentration in healthy cells, although this may be an indirect effect. In animal models for CF, such as the chronically reserpinized rat and the chronically isoproterenol-treated rat, abnormally high calcium levels in the acinar cells of the submandibular gland could be demonstrated, similar to the situation in CF patients. In the acinar cells of the parotid gland in these animal models, the calcium levels are, however, abnormally low. This suggests that the changes in cell calcium content are secondary to other changes, possibly changes in the secretory proteins. A study of the effect of the serum calcium level and of the calciotropic hormone calcitonin suggested that neither of these factors could be directly linked with CF. It is concluded that several lines of evidence point to a secondary rather than a primary role for calcium in the pathogenesis of CF.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 1","pages":"165-78"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14857577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlative scanning electron microscopy in the study of human gastric mucosa. 相关扫描电镜在人胃粘膜研究中的应用。

Scanning electron microscopy Pub Date : 1986-01-01

F Bonvicini, M C Maltarello, P Versura, D Bianchi, G Gasbarrini, R Laschi

{"title":"Correlative scanning electron microscopy in the study of human gastric mucosa.","authors":"F Bonvicini, M C Maltarello, P Versura, D Bianchi, G Gasbarrini, R Laschi","doi":"","DOIUrl":"","url":null,"abstract":"We studied two aspects of the human gastric mucosa: the surface morphology of mucous cells, as viewed by scanning electron microscopy (SEM); the glycosidic components of intracellular mucins, characterized by means of lectins. The latter were conjugated with fluorescein isothiocyanate and with colloidal gold-silver for the visualization of the reaction products in light microscopy (LM) and in SEM (backscattered mode) respectively. The surface morphology of mucous cells appears to be correlated to the secretory state. In gastric ulcers we found a prevalence of non-secreting cells. A decrease in glycosidic receptors for fucose-binding lectin and galactose-(1-3)-N-acetyl-galactosamine-binding lectin was also observed. This suggests the presence of an impaired mucus secretion which may play a role in the pathogenesis of gastric ulcer. Spiral bacteria, supposed to be aetiologically related to peptic ulcer and gastritis, were easily detected by SEM. Intestinal metaplasia defined \"complete\" in LM showed surface morphology and glycosidic components different from those of true intestinal mucosa. This implies the necessity of taking into account also these parameters when classifying this lesion. The same applies to polyps. Our data indicate that correlative SEM may contribute further information on the pathogenesis and pathology of gastric diseases.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 2","pages":"687-702"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14231075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene and teratogen induced defects of early central nervous system development. 基因和致畸原诱导的早期中枢神经系统发育缺陷。

Scanning electron microscopy Pub Date : 1986-01-01

K S O'Shea

引用次数: 0

Surface ultrastructure of human megakaryocytes sorted on the basis of DNA content. 根据DNA含量分选的人巨核细胞表面超微结构。

Scanning electron microscopy Pub Date : 1986-01-01

M A Weller, J Szela, M I Barnhart, A Nakeff

引用次数: 0

Intracellular structure of the outer hair cell of the organ of Corti. 器官外毛细胞的胞内结构。

Scanning electron microscopy Pub Date : 1986-01-01

Y Harada, T Sakai, N Tagashira, M Suzuki

引用次数: 0

Exocytosis from large dense cored vesicles as a mechanism for neuropeptide release in the peripheral and central nervous system. 外周和中枢神经系统神经肽释放的一种机制:来自大而致密的囊泡的胞吐。

Scanning electron microscopy Pub Date : 1986-01-01

A Thureson-Klein, R L Klein, P C Zhu

{"title":"Exocytosis from large dense cored vesicles as a mechanism for neuropeptide release in the peripheral and central nervous system.","authors":"A Thureson-Klein, R L Klein, P C Zhu","doi":"","DOIUrl":"","url":null,"abstract":"Nerve terminals often contain morphologically distinct populations of large (75-110 nm) and small (45-55 nm) vesicles. The small vesicles are speculated to account for release of transmitter quanta as they accumulate at presynaptic membranes. Large vesicles can co-store neuropeptides and classical transmitters but their function in neurotransmission has been disputed because they do not appear to accumulate at chemical synapses. However, there is now evidence that the large vesicles play a role in neurotransmission or its modulation even though they may not be eminently involved in synaptic release. Thus, exocytosis occurs along the synapse-lacking membranes of peripheral noradrenergic varicosities. Large vesicles may continue to function in peptide release even after the classical transmitter has been depleted as demonstrated in the pig vas deferens. Three days of reserpine administration causes a parallel loss of noradrenaline and small vesicle contents but does not decrease enkephalin-like immunoreactivity or large vesicle electron density. In the central nervous system of the rat, where substance P and enkephalin have been localized to large vesicles, exocytosis occurs from several types of terminals. The large vesicles appear preferentially to release their contents at morphologically non-specialized sites even when characteristic synapses are present. Thus different mechanisms of transmitter and neuropeptide release may coexist. The nonsynaptic discharge may allow substances to diffuse over a wider distance whereas release into a synaptic cleft could restrict receptor interaction.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 1","pages":"179-87"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14874376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The enamel ultrastructure of multituberculate mammals: a review. 多瘤哺乳动物牙釉质超微结构研究进展。

Scanning electron microscopy Pub Date : 1986-01-01

D W Krause, S J Carlson

{"title":"The enamel ultrastructure of multituberculate mammals: a review.","authors":"D W Krause, S J Carlson","doi":"","DOIUrl":"","url":null,"abstract":"The enamel ultrastructure of multituberculate mammals has been sampled extensively and studied intensively and is better known than for any other group of early mammals. The enamel of the earliest multituberculates, those of the Late Triassic-Early Jurassic suborder Haramiyoidea and the Late Jurassic-early Early Cretaceous suborder Plagiaulacoidea, is \"preprismatic.\" With only two exceptions, all Late Cretaceous and early Tertiary genera of multituberculates examined have prismatic enamel. Prisms are either small with circular (complete) boundaries or large with arc-shaped (incomplete) boundaries. There is a remarkably consistent relationship between enamel ultrastructural type and subordinal taxa in that small, circular prisms are usually found within the suborder Ptilodontoidea and large, arc-shaped prisms are usually found in the suborder Taeniolabidoidea and in six Late Cretaceous-Early Tertiary genera of indeterminate subordinal status. Research currently in progress suggests that both small, circular prisms and large, arc-shaped prisms are homologous in all multituberculates in which they occur, with one exception. Neoliotomus, a taeniolabidoid, appears to have evolved small, circular prisms independently. In addition, it appears that large, arc-shaped prisms represent the primitive condition in multituberculates with prismatic enamel, not small, circular prisms as has been proposed previously.","PeriodicalId":21455,"journal":{"name":"Scanning electron microscopy","volume":" Pt 4","pages":"1591-607"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14928795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0