Russian Journal of Developmental Biology最新文献

Generation of an Induced Pluripotent Stem Cell Line ICGi045-A of a RASopathy Patient Carrying p.Glu329Lys Variant in SOS1 生成携带 SOS1 中 p.Glu329Lys 变异的 RAS 病患者的诱导多能干细胞系 ICGi045-A

IF 0.7 4区生物学

Russian Journal of Developmental Biology Pub Date : 2024-07-18 DOI: 10.1134/s1062360424700036

E. V. Dementyeva, A. K. Zaytseva, J. M. Minina, O. V. Melnik, S. M. Zakian, A. A. Kostareva

引用次数: 0

Fatty Acid Content of Phospholipids and Triacylglycerols in Juvenile Atlantic Salmon Salmo salar L. Reared under Different Lighting and Feeding Regimes in Aquaculture (North Ossetia-Alania) 水产养殖中不同光照和饲养条件下饲养的大西洋鲑鱼幼鱼磷脂和三酰甘油的脂肪酸含量（北奥塞梯-阿拉尼亚地区）

IF 0.7 4区生物学

Russian Journal of Developmental Biology Pub Date : 2024-07-18 DOI: 10.1134/s1062360424700012

S. N. Khurtina, S. A. Murzina, D. S. Provotorov, V. P. Voronin, A. E. Kuritsyn, N. N. Nemova

{"title":"Fatty Acid Content of Phospholipids and Triacylglycerols in Juvenile Atlantic Salmon Salmo salar L. Reared under Different Lighting and Feeding Regimes in Aquaculture (North Ossetia-Alania)","authors":"S. N. Khurtina, S. A. Murzina, D. S. Provotorov, V. P. Voronin, A. E. Kuritsyn, N. N. Nemova","doi":"10.1134/s1062360424700012","DOIUrl":"https://doi.org/10.1134/s1062360424700012","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The photoperiod is an important ecological factor synchronizing the endogenous rhythms of growth and development of the Atlantic salmon <i>Salmo salar</i> L. The changes in the FA content of phospholipids (PL) and triacylglycerols (TAG) during the growth and development of Atlantic salmon juveniles (fingerlings, parrs, and smolts) at different lighting and feeding regimes in aquaculture of the southern region (North Ossetia, Alania) were studied. A high content of saturated fatty acids (SFA) and a consistently high content of polyunsaturated fatty acids (PUFA) in PL (more than 40% of the total FA) were detected. An increase in the content of PUFA in TAG both in the salmon liver and in muscles during development from fingerlings to smolts indicated the biochemical changes in the fish organism preparing for smoltification and the transition to living in sea water. Among PUFA, n-3 PUFA dominated, while the content of n-6 PUFA was no more than 5% both in PL and TAG of juvenile salmon. At the same time, among PUFA the content of eicosapentaenoic acid 20:5(n-3) (EPA) in the muscles of both parrs and smolts was higher than in the liver, where docosapentaenoic acid 22:5(n-3) (DPA) dominated. The results of quantitative and qualitative changes in the FA components of lipids in juvenile salmon depending on changes in the lighting and feeding regimes are important for understanding the mechanisms of ecological and biochemical adaptations that determine the early development of salmon and its transition to the smoltification.</p>","PeriodicalId":21434,"journal":{"name":"Russian Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141737286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Creation of Induced Pluripotent Stem Cells RCMGi014-A Using Reprogramming of Urine Cells of a Patient with Fibrodysplasia Ossificans Progressiva Associated with Heterozygous Mutation in the ACVR1 Gene 利用对一名伴有 ACVR1 基因杂合子突变的肌纤维增生症患者的尿液细胞进行重编程，创建诱导多能干细胞 RCMGi014-A

IF 0.7 4区生物学

Russian Journal of Developmental Biology Pub Date : 2024-07-18 DOI: 10.1134/s1062360424700048

E. V. Kondrateva, O. V. Grigorieva, E. V. Kurshakova, I. O. Panchuk, V. O. Pozhitnova, E. S. Voronina, V. Yu. Tabakov, I. P. Nikishina, S. V. Arsenyeva, V. G. Matkava, A. V. Lavrov, S. A. Smirnikhina, S. I. Kutsev

引用次数: 0

Three Foxg1 Genes in Lampreys: The Heritage of Whole-Genome Duplications at the Early Stages of Vertebrate Evolution 灯笼鱼的三个 Foxg1 基因：脊椎动物进化早期全基因组重复的遗产

IF 0.7 4区生物学

Russian Journal of Developmental Biology Pub Date : 2024-07-18 DOI: 10.1134/s1062360424700024

G. V. Ermakova, A. V. Kucheryavyy, A. G. Zaraisky, A. V. Bayramov

引用次数: 0

Molecular and Cellular Mechanisms Governing Adipogenic Differentiation 控制脂肪分化的分子和细胞机制

IF 0.7 4区生物学

Russian Journal of Developmental Biology Pub Date : 2024-03-23 DOI: 10.1134/s1062360423070068

V. Y. Sysoeva, M. A. Lazarev, K. Y. Kulebyakin, E. V. Semina, K. A. Rubina

{"title":"Molecular and Cellular Mechanisms Governing Adipogenic Differentiation","authors":"V. Y. Sysoeva, M. A. Lazarev, K. Y. Kulebyakin, E. V. Semina, K. A. Rubina","doi":"10.1134/s1062360423070068","DOIUrl":"https://doi.org/10.1134/s1062360423070068","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Adipose tissue is a special type of connective tissue that comprises stromal-vascular cells, committed preadipocytes, and mature adipocytes. Adipose tissue exhibits high plasticity and is capable of both rapid physiological expansion and reduction in response to dietary changes. Under normal conditions, an increase in adipose tissue volume occurs through hyperplasia, while tissue expansion in obesity results from hypertrophy of existing adipocytes. Adipose tissue cells not only store energy in the form of triglycerides but also secrete crucial adipokines, regulating the overall body metabolism. In metabolic syndrome and obesity, alterations affect not only adipose tissue morphology but also adipocyte renewal and differentiation as well as the hormonal and secretory functions. The fundamental mechanisms regulating cell renewal and differentiation in adipose tissue warrant detailed investigation to comprehend the metabolic processes in the entire organism, both under normal and pathological conditions. This review provides insights into the principal regulators governing the transcriptional program that controls adipogenesis, as well as the key signaling cascades that influence adipocyte differentiation.</p>","PeriodicalId":21434,"journal":{"name":"Russian Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of an Induced Pluripotent Stem Cell Line ICGi046-A of a Patient Carrying Pathogenic p.Ser259Thr Variant in RAF1 Associated with RASopathy 诱导多能干细胞系 ICGi046-A 的产生：一名携带与 RAS 病相关的 RAF1 致病性 p.Ser259Thr 变体的患者

IF 0.7 4区生物学

Russian Journal of Developmental Biology Pub Date : 2024-03-23 DOI: 10.1134/s1062360423070032

E. V. Dementyeva, E. S. Klimenko, J. M. Minina, S. M. Zakian, A. A. Kostareva

引用次数: 0

Generation of an Induced Pluripotent Stem Cell Line TNRMCi001-A by Reprogramming Fibroblasts from a Homozygous F508del Cystic Fibrosis Patient 通过重编程一名同型 F508del 囊性纤维化患者的成纤维细胞，生成诱导多能干细胞系 TNRMCi001-A

IF 0.7 4区生物学

Russian Journal of Developmental Biology Pub Date : 2024-03-23 DOI: 10.1134/s106236042307007x

D. I. Zhigalina, T. N. Kireeva, T. V. Nikitina, O. N. Odinokova, N. A. Kolesnikov, A. A. Malakhova, R. R. Savchenko, I. Zh. Zhalsanova, N. R. Valiahmetov, A. E. Postrigan, S. L. Vovk, N. B. Torkhova, A. A. Frolova, V. A. Stepanov, N. A. Skryabin

{"title":"Generation of an Induced Pluripotent Stem Cell Line TNRMCi001-A by Reprogramming Fibroblasts from a Homozygous F508del Cystic Fibrosis Patient","authors":"D. I. Zhigalina, T. N. Kireeva, T. V. Nikitina, O. N. Odinokova, N. A. Kolesnikov, A. A. Malakhova, R. R. Savchenko, I. Zh. Zhalsanova, N. R. Valiahmetov, A. E. Postrigan, S. L. Vovk, N. B. Torkhova, A. A. Frolova, V. A. Stepanov, N. A. Skryabin","doi":"10.1134/s106236042307007x","DOIUrl":"https://doi.org/10.1134/s106236042307007x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Cystic fibrosis (CF) is a hereditary disease that leads to impaired functioning of chloride channels in cells, and, as a result, to a decrease in the viscoelastic properties of the secretion of all exocrine glands. Cystic fibrosis is the result of mutations in the cystic fibrosis transmembrane conductance regulator (<i>CFTR</i>) gene, which encodes the CFTR protein. In this study, induced pluripotent stem cells (iPSCs) were obtained from the skin fibroblasts of a patient with a homozygous mutation F508del <i>CFTR</i> (NM_000492.3(CFTR):c.1521_1523del). This deletion is the most common for cystic fibrosis. The resulting iPSC line had a normal karyotype, retained the original genotype, and also demonstrated the presence of pluripotency markers (OCT4, SOX2, NANOG, SSEA4, TRA-1-60) and the ability to differentiate into derivatives of three germ layers.</p>","PeriodicalId":21434,"journal":{"name":"Russian Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Distribution of the Germ Plasm Gene germes among Anurans 无尾类种质基因胚芽的分布

IF 0.7 4区生物学

Russian Journal of Developmental Biology Pub Date : 2024-03-07 DOI: 10.1134/s106236042306005x

V. V. Konduktorova, E. G. Fofanova, D. A. Nikishin

{"title":"The Distribution of the Germ Plasm Gene germes among Anurans","authors":"V. V. Konduktorova, E. G. Fofanova, D. A. Nikishin","doi":"10.1134/s106236042306005x","DOIUrl":"https://doi.org/10.1134/s106236042306005x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The <i>germes</i> gene is a marker of germ plasm and primordial germ cells (PGC). It is described for the African clawed frog <i>Xenopus laevis</i>. Overexpression of its mutant form negatively affects the formation and migration of PGC. However, it was unknown until now how widely this gene is represented in animals of different phylogenetic groups. For this work, the authors performed bioinformatic analysis of genomic and transcriptome sequences of animals with germ plasm. We found out that the <i>germes</i> homologs are present only in representatives of the genera <i>Xenopus</i> and <i>Hymenochirus</i> of the family Pipidae (order Anura). The obtained results were confirmed by RT-PCR analysis of the expression of the <i>germes</i> orthologs in the ovaries of six representatives of different Anura families. Phylogenetic analysis of cloned sequences of the <i>germes</i> homologs suggests the appearance of this gene in the ancestors of Pipidae and its secondary loss in the genus <i>Pseudohymenochirus</i>. It is also shown that the amino acid sequences of the functional domains of the Germes protein are rather conservative.</p>","PeriodicalId":21434,"journal":{"name":"Russian Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140071710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ectopic Expression of Human, Mouse, and Fruit Fly Aryl Hydrocarbon Receptor in Drosophila melanogaster Tissues 人、小鼠和果蝇芳香烃受体在黑腹果蝇组织中的异位表达

IF 0.7 4区生物学

Russian Journal of Developmental Biology Pub Date : 2024-03-07 DOI: 10.1134/s1062360423060036

D. Yu. Baranova, A. A. Akishina, R. O. Cherezov, D. A. Kulikova, I. B. Mertsalov, O. B. Simonova, J. E. Vorontsova

引用次数: 0

Transgenic Lines of Human Induced Pluripotent Stem Cells ICGi022-A-6 and ICGi022-A-7 with Doxycycline-Inducible Variants of Programmable Nuclease AsCas12a 用强力霉素诱导的可编程核酸酶 AsCas12a 变异株转基因人诱导多能干细胞 ICGi022-A-6 和 ICGi022-A-7

IF 0.7 4区生物学

Russian Journal of Developmental Biology Pub Date : 2024-03-07 DOI: 10.1134/s1062360423060061

S. V. Pavlova, K. R. Valetdinova, T. B. Malankhanova, D. E. Polivtsev, A. A. Malahova, E. V. Grigor’eva, A. I. Shevchenko, S. M. Zakian, S. P. Medvedev

{"title":"Transgenic Lines of Human Induced Pluripotent Stem Cells ICGi022-A-6 and ICGi022-A-7 with Doxycycline-Inducible Variants of Programmable Nuclease AsCas12a","authors":"S. V. Pavlova, K. R. Valetdinova, T. B. Malankhanova, D. E. Polivtsev, A. A. Malahova, E. V. Grigor’eva, A. I. Shevchenko, S. M. Zakian, S. P. Medvedev","doi":"10.1134/s1062360423060061","DOIUrl":"https://doi.org/10.1134/s1062360423060061","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Genome editing in human pluripotent stem cells using programmable nucleases makes it possible to create models of hereditary pathologies using directed transgenesis, gene knockout, and replacement of individual nucleotides in DNA sequences. Using CRISPR/SpCas9-mediated homologous recombination at the AAVS1 locus, clones of human induced pluripotent stem cells (iPSCs) ICGi022-A (Malakhova et al., 2020) were obtained, which carry transgenes of two variants of the nuclease AsCas12a (also known as AsCpf1), recognizing different PAM consensuses, and the reverse doxycycline transgene-dependent transactivator M2rtTA. For each AsCas12a variant, the lines ICGi022-A-6 (AsCas12a, PAM 5'-TTTV-3') and ICGi022-A-7 (AsCas12a, PAM 5'-TYCV-3') were obtained. Using Western blot analysis, it was shown that the addition of doxycycline to the culture medium causes activation of the expression of AsCas12a(TTTV) and AsCas12a(TYCV) proteins. The resulting transgenic iPSC clones were subjected to molecular and cytogenetic analysis. Using quantitative PCR and immunocytochemical analysis, it was shown that they have a high level of mRNA expression of gene markers of pluripotent cells, namely <i>OCT4, NANOG</i>, and <i>SOX2</i>, as well as specific expression of protein markers OCT4, SOX2, SSEA-4, and TRA-1-60. In addition, using iPSCs spontaneous differentiation into embryoid bodies, it was found that transgenic clones can give derivatives of all three primitive germ layers: ectoderm, mesoderm, and endoderm. Cytogenetic analysis showed that transgenic iPSC clones have a normal karyotype, 46,XX.</p>","PeriodicalId":21434,"journal":{"name":"Russian Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140071633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0