Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove最新文献_第2页

Proportional changes in the facial part of the skull of immature individuals. 未成熟个体颅骨面部的比例变化。

Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove Pub Date : 1995-01-01

Z Hodacová, H Skalská

引用次数: 0

Cerebrospinal fluid dissemination of fetal neural isografts in brain of adult rats. 成年大鼠脑内胎儿神经同种移植物的脑脊液播散。

Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove Pub Date : 1995-01-01

S Nĕmecek, J Mokrý, Y Mazurová, D Subrtová

引用次数: 0

Immunohistochemical detection of proliferative cells. 增殖细胞的免疫组化检测。

Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove Pub Date : 1995-01-01

J Mokrý, S Nĕmecek

{"title":"Immunohistochemical detection of proliferative cells.","authors":"J Mokrý, S Nĕmecek","doi":"","DOIUrl":"","url":null,"abstract":"The cell proliferation represents one of the most relevant cellular functions. There are many approaches to assess the proliferative activity in tissues. Immunohistochemical methods offer a high sensitivity and specificity. They use monoclonal antibodies raised against specific antigens associated with the cell proliferation. Anti-BrdU immunohistochemistry detects bromodeoxyuridine (BrdU) which as an exogenous proliferation marker has been incorporated into the newly synthesized DNA of dividing cells. The best known endogenous proliferation markers are PCNA and Ki-67 that may be detected with specific commercially available antibodies. Here we describe our experience with an immunohistochemical detection of these proliferation markers. Anti-bromodeoxyuridine (BrdU) antibody binds BrdU that was exogenously introduced into the synthesizing DNA during the S-phase of the cell cycle. The precise timing and dosage of BrdU enable tissue kinetics studies. A long-time administration of BrdU may be used for labelling tissues with a low proliferative activity. BrdU incorporated into the cell nucleus is still a very stable antigen which gives a strong and reliable signal regardless the kind of fixation and further tissue processing. The proliferating cell nuclear antigen (PCNA) as an auxillary protein for DNA polymerase gamma is an evolutionarily conserved molecule that may be detected in human and animal frozen or paraffin-embedded tissues. In spite of the fact that anti-PCNA immunohistochemistry may weakly stain non-proliferating cells, PCNA is the most frequently detected proliferation marker. The staining intensity may be influenced by the kind of fixation and the length of microwave pretreatment. Ki-67 is another endogenous antigen detectable in proliferating cells. Its detection in paraffin-embedded sections requires also exposition to microwaves. Both Ki-67 and PCNA may be revealed in archival specimens that means these techniques do not require any prelabelling. Ki-67 labelling index (LI) correlates better with BrdU LI than PCNA LI as the Ki-67 antigen has a shorter half-life than PCNA.","PeriodicalId":21432,"journal":{"name":"Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove","volume":"38 3","pages":"107-13"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19660226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in microvascularization of some rat organs following soman poisoning. 苏曼中毒后一些大鼠器官微血管化的变化。

Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove Pub Date : 1995-01-01

D Slízová, O Krs, J Bajgar, B Pospísilová

引用次数: 0

Epilepsy associated with low-grade brain glial neoplasms. 癫痫与低级别脑胶质肿瘤相关。

Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove Pub Date : 1995-01-01

J Nĕmecková, S Nĕmecek, J Náhlovský, J Parízek, M Sercl

{"title":"Epilepsy associated with low-grade brain glial neoplasms.","authors":"J Nĕmecková, S Nĕmecek, J Náhlovský, J Parízek, M Sercl","doi":"","DOIUrl":"","url":null,"abstract":"In 1991-1993, 52 patients underwent surgery for low-grade supratentorial glioma. In 37 of them (astrocytoma 22, oligodentrocytoma 12, oligodendroglioma 2) seizures, often refractory to drug therapy, appeared as the first symptom. These cases were retrospectively analyzed. The patients had partial seizures: simple, complex, or secondarily generalized (preoperative duration: from 3 days to 17 years (mean 2 years); frequency: between 1 and 2/year and over 10/day). Neurological examination either revealed slight focal changes or was normal. Conventional craniotomy and resection of a tumor, without intraoperative electrocorticography, was performed. Partial resection was performed in 73%, subtotal in 5%, \"total\" in 22% of the cases. Postoperatively, 27 patients had focal radiotherapy, 3 of them in combination with chemotherapy. Two patients were reoperated. Out of 33 alive (89%), about two-thirds appear normal by neurological examination and are seizure-free at present (mean follow-up period 28 months). Most remain on antiepileptic drugs at lower doses. Histological and immunohistochemical analyses of resected tissue together suggest that the peripheral zone of cortical tumor infiltration may participate on epileptogenesis.","PeriodicalId":21432,"journal":{"name":"Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove","volume":"38 4","pages":"175-83"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19668776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum level of IL-6 in patients with primary and secondary Sjögren's syndrome. 原发性和继发性Sjögren综合征患者血清IL-6水平的变化。

Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove Pub Date : 1995-01-01

R Slezák, J Krejsek, O Kopecký, H Jelenová

引用次数: 0

Convulsive properties of N-(3,5-dimethoxy-4-propoxyphenyl-ethyl) aziridine and their influencing by diazepam and triazolam. N-(3,5-二甲氧基-4-丙氧基苯基-乙基)氮吡啶的惊厥性质及安定和三唑仑对其的影响。

Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove Pub Date : 1995-01-01

J Herink

引用次数: 0

Impedance monitoring in the stereotactic localization of intracranial structures. 阻抗监测在颅内结构立体定位中的应用。

Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove Pub Date : 1995-01-01

V Hobza, J Jakubec, J Nĕmecková, S Nĕmecek, M Sercl

引用次数: 0

Extraadrenal pheochromocytoma. Extraadrenal嗜铬细胞瘤。

Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove Pub Date : 1995-01-01

J Base, J Sváb

引用次数: 0

Histochemical localization of succinate dehydrogenase in intracerebral mouse submandibular gland transplants. 鼠脑内颌下腺移植后琥珀酸脱氢酶的组织化学定位。

Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove Pub Date : 1995-01-01

B Pospisilová, O Procházková, I Kubisová, D Slizovă, P Bílek

{"title":"Histochemical localization of succinate dehydrogenase in intracerebral mouse submandibular gland transplants.","authors":"B Pospisilová, O Procházková, I Kubisová, D Slizovă, P Bílek","doi":"","DOIUrl":"","url":null,"abstract":"Histochemical localization of succinate dehydrogenase in developing intracerebral auto- and homotransplants of the mouse submandibular gland was investigated in the course of a 5-month period after transplantation. Eight weeks after grafting, the enzyme pattern in the parenchyma of homotransplants was comparable with the histochemical picture of a fully mature submandibular gland in situ. At this time, numerous acini showed a weak activity, very numerous striated ducts a strong activity, and less frequent developing convoluted granular tubules slightly weaker activity, than in the striated ducts. Beginning histochemical differentiation of convoluted granular tubules was noted only in homotransplants, located in the brain of male recipients. On the other hand, only a weak activity in the cytoplasm of non-differentiated duct--like structures of some autotransplants was seen. Homotransplants of non-differentiated submandibular gland of newborn donors were found to be a more suitable transplantation object capable of postnatal development of gland parenchyma than autografts of fully differentiated gland of adult animals that did not enter the cytodifferentiation stage.","PeriodicalId":21432,"journal":{"name":"Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove","volume":"38 2","pages":"71-7"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19565239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0