Russian Open Medical Journal最新文献

{"title":"Examining Apoptotic Activity Of Gratiola Officinalis L. (Scrophulariaceae) Extract On Cultured Human Tumor Cell Lines","authors":"N. Polukonova, N. Navolokin, M. Baryshnikova, G. Maslyakova, A. Bucharskaya, A. Polukonova","doi":"10.15275/rusomj.2022.0415","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0415","url":null,"abstract":"Objective — To investigate the apoptotic activity of Gratiola officinalis L. extract on human tumor cell lines by flow cytofluorometry. Material and Methods — The extract of Gratiola officinalis L. was manufactured via our original methodology. Studies were performed on human tumor cell lines: HeLa – cervical carcinoma, Jurkat – T-cell lymphoblastic leukemia, MCF-7 – breast adenocarcinoma, A549 – lung carcinoma, PC-3 – prostate carcinoma, HCT-116 – colon carcinoma, A498 – renal carcinoma, and SK-BR-3 – human breast carcinoma. Induction of apoptosis was studied after incubating cell lines with Gratiola officinalis L. extract at a concentration of 0.9 mg/mL using the Annexin V-FITC Apoptosis Kit. Caspase-dependent apoptosis was examined on a flow cytometer using anti-caspase-3-FITC (BD) kit on the Jurkat cell line. Morphological studies of HeLa cervical carcinoma cells in the alive and dead test were performed using two stains, acridine orange and propidium iodide, at different concentrations of the extract. The statistical data processing was performed using Microsoft Office Excel software. Results — One day after their exposure to Gratiola officinalis L. extract at a concentration of 0.9 mg/mL, tumor cells were mostly in late apoptosis stage. Cytotoxic activity of Gratiola officinalis L. extract was established for all investigated tumor cell cultures but their sensitivities to the extract were different. Mechanisms of antitumor action of Gratiola officinalis L. extract were identified: we established that the extract induced caspase-dependent apoptosis in tumor cells. Conclusion — The identified mechanisms of apoptotic activity of Gratiola officinalis L. extract confirmed the prospects of bioflavonoids as new-generation antitumor agents.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48659958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect Of Adaptation Duration To Environmental Conditions Of The North On Morphofunctional Restructuring Of Capillary Blood Flow","authors":"I. Averyanova, S. Vdovenko","doi":"10.15275/rusomj.2022.0304","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0304","url":null,"abstract":"Background — We studied blood microcirculation and features of capillary morphology in young men with various duration of adaptation to the environmental conditions of Northeast Russia (Magadan Oblast). Methods — We examined 211 young men 17-21 years of age who were permanent residents of the North. All study subjects were divided among four groups: Caucasian migrants from central Russia (generation 0) and those born and residing in the North, representing generations 1, 2, and 3. We investigated capillary structure and microcirculation in the eponychium of the nail bed, using a computer-based video capillaroscope, Capillaroscan-1. Results — We detected the diameter reduction in arterial and intermediate capillary segments with simultaneous absence of a similar pattern in the venous capillary segment along the gradient of generation 0 towards generation 3. We also observed a shorter mean capillary length against the background of a thicker capillary network. Conclusion — With a longer adaptation period to the environmental conditions of Northeast Russia, compensatory adaptive mechanisms in the capillary network structure and microcirculation are formed, aimed at optimizing capillary blood flow.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46813738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role Of P-Selectin, Hemopexin, Lactoferrin, Iron And Ferritin In Patients With Giardiasis And Amoebiasis: A Narrative Review","authors":"S. K. Al-Hadraawy, A. H. Abood, R. Zabibah, Ameer A. Imarah, A. Almulla, Ali Abbas Abo Algon","doi":"10.15275/rusomj.2022.0314","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0314","url":null,"abstract":"The most frequent intestinal parasites that cause severe disorders in humans are Giardia lamblia and Entamoeba histolytica, which alter serum concentrations of deferent markers due to virulence factors and pathogenicity. A large number of people with infection are asymptomatic, and they can go for up to a year without showing any signs or symptoms. Additionally, due to prolonged diarrhoea but not acute diarrhoea, these parasites can cause malnutrition, weight loss, growth delay, and possibly low cognitive development. The aim of this study is to look at how giardiasis and amoebiasis affect the levels of certain biomarkers in the blood.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49341082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy Of Intradermal Allogeneic Fibroblast Injections In Junctional Epidermolysis Bullosa","authors":"A. Kubanov, A. Karamova, V. Chikin, E. Monchakovskaya, Mariya A. Nefedova","doi":"10.15275/rusomj.2022.0315","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0315","url":null,"abstract":"Objective — to assess the efficacy and safety of intradermal injections of allogeneic fibroblasts into non-healing wounds in a patient with junctional epidermolysis bullosa. Material and Methods — A 49-year-old patient with intermediate junctional epidermolysis bullosa was injected intradermally into the base of non-healing wounds with 1 mL suspension of allogeneic fibroblasts, which contained 5×106 cells/mL, 10×106 cells/mL, and 20×106 cells/mL. Immunofluorescence mapping exhibited reduced β3 chain of laminin 332 and collagen XVII expression in the basement membrane area. Paired erosions were injected with 2% albumin or saline solution. Results — At two weeks after treatment, wound areas reduced significantly, or 100% re-epithelialization occurred. Collagen XVII and β3 chain expression of laminin 332 increased at the dermal-epidermal junction. Conclusion — Our findings demonstrated that intradermal injections of allogeneic fibroblasts could be an effective therapeutic approach for treating small non-healing wounds in junctional epidermolysis bullosa.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":"27 4","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41288172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure Of Comorbidity In Urban Population With Essential Hypertension In The Clinical Practice Of A Local General Practitioner","authors":"E. Osmanov, R. Manyakov, A. Garaeva, L. Tuktamysheva, L. H. Korkmazova, I. Arsanukaev, Jasmina A. Garaeva, Arthur A. Antonov","doi":"10.15275/rusomj.2022.0307","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0307","url":null,"abstract":"Taking into account gender- and age-related features, to examine the structure of comorbidity in outpatients with essential hypertension residing in the city of Tambov. Material and Methods — This research was an observational cross-sectional study based on data extracted from 47,113 electronic medical records (EMRs) of patients 20-99 years of age residing in Tambov. The study included 29,282 (62.2%) women and 17831 (37.8%) men. Results — Essential hypertension is habitually associated with osteochondrosis (41.5% of patients with hypertension), cerebrovascular diseases (35.1%), arthrosis (28.3%), ischemic heart disease (IHD) (28.7%), menopausal disorders (17.5%), pancreatic diseases (15.4%), diabetes mellitus (14.7%), benign mammary dysplasia (14.2%), retinal diseases (12.9%), disorders of lipoprotein metabolism (12.4%), senile cataract (10.6%), gastritis and duodenitis (10.7%), varicose veins (10.1%), and thyroid diseases (10.0%). IHD is more common among men with hypertension, while other comorbidities are more characteristic for women. The most striking gender-related difference was associated with the finding that women with hypertension had varicose veins as a comorbidity (RR=1.398; 95% CI 1.376-1.422), while the smallest difference between genders related to retinal diseases (RR=1.065; 95% CI 1.038-1.092). IHD was strongly associated with the male gender (RR=1.101; 95% CI 1.056-1.148). Three diseases were more often associated with hypertension in the age range of 80-89 years (cerebrovascular disease, IHD, osteochondrosis); some of the diseases had the highest incidence in the age range of 60-69 years, and other disorders occurred in the age range of 40-59 years. Conclusion — The gender- and age-related features of comorbid pathology associated with essential hypertension among urban residents revealed in this study are extremely important in the clinical practice of a local general practitioner for developing optimal patient-oriented treatment plans.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43569557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction Of Immune Response Mediator Genes In A Predisposition To Juvenile Idiopathic Arthritis","authors":"L. Nazarova, K. Danilko, V. Malievsky, D. Karimov, A. Bakirov, T. Viktorova","doi":"10.15275/rusomj.2022.0311","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0311","url":null,"abstract":"Background/objective — The goal of our study was to investigate the role of interaction between the polymorphic loci of immune response mediator genes (TNFA rs1800629, LTA rs909253, IL1B rs16944, IL2-IL21 rs6822844, IL2RA rs2104286, IL6 rs1800795, IL10 rs1800872, MIF rs755622, CTLA4 rs3087243, NFKB1 rs28362491, PTPN22 rs2476601, and PADI4 rs2240336) in the formation of a genetic predisposition to juvenile idiopathic arthritis (JIA). Material and Methods — The study involved 330 JIA patients and 342 volunteers from the Republic of Bashkortostan. Genotyping was conducted via the real-time polymerase chain reaction. The gene-gene interactions were studied using the multifactor dimensionality reduction algorithm. Results — In general analysis, the best model of gene-gene interaction in JIA was a combination of IL1B rs16944 – IL10 rs1800872 – NFKB1 rs28362491 – PADI4 rs2240336 polymorphic loci. However, after gender-based stratification the best results were obtained when examining the combinations of IL6 rs1800795 – PADI4 rs2240336 loci in girls and of IL10 rs1800872 – IL6 rs1800795 – IL2RA rs2104286 loci in boys. Within all of these models, the genotype combinations associated with both augmented and reduced JIA risks were identified (taking into account gender-specific differences). Conclusion — The results of our study implied that an important role in the formation of a predisposition to JIA is played by gene-gene interactions of IL1B rs16944, IL2RA rs2104286, IL6 rs1800795, IL10 rs1800872, NFKB1 rs28362491, and PADI4 rs2240336 polymorphic loci (taking into account gender-specific differences).","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46468332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenetic Mechanisms Of Dry Eye Syndrome In A Novel Coronavirus Infection Caused By SARS-CoV-2","authors":"T. Safonova, G. Zaitseva","doi":"10.15275/rusomj.2022.0306","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0306","url":null,"abstract":"The goal of this review was to analyze current knowledge on dry eye disease pathogenesis in a novel coronavirus infection (COVID-19) caused by SARS-CoV-2. Arguments are presented in favor of several possible pathogenic mechanisms of the disease development: inflammation and/or microcirculatory disorders aggravated by exposure to electromagnetic radiation of personal computers and by use of personal protective equipment.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47497060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis Of Immunosuppressive Therapy Effectiveness In COVID-19 Patients","authors":"A. Tyurin, Karina E. Akhiyarova, D.A. Valishin, L. D. Sadretdinova, L. N. Khusainova, N. Zagidullin, K. Gantseva, V. Pavlov","doi":"10.15275/rusomj.2022.0312","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0312","url":null,"abstract":"The objective of our study was the analysis of using immunosuppressive therapy in patients with COVID-19 at the Clinic of the Bashkir State Medical University. Material and methods — We conducted the analysis of clinical and laboratory parameters of inflammatory response in 322 patients with COVID-19 who received tocilizumab, baricitinib, high doses of dexamethasone, or standard therapy. Results — There was an increase in the levels of leukocytes (p=0.04) and neutrophils (p=0.002) in patients receiving tocilizumab, compared with standard therapy, on days 5 and 10 of a hospital stay. The level of C-reactive protein was initially elevated in all patients, but by day 5 of hospitalization it was significantly higher in patients treated with tocilizumab and baricitinib (p=0.0019 and p=0.013, respectively), compared with high-dose glucocorticoid therapy and standard treatment, against which the normalization of parameter values was noted. The neutrophil-to-lymphocyte ratio increased in the group of patients receiving tocilizumab and high-dose glucocorticoid therapy on day 5 of hospitalization (p=0.017 and p=0.004). When assessing the dynamics of pneumonia, based on computed tomography data, the median of changes exhibited an increase in the volume of lung damage in all groups, compared with the baseline level. Conclusion — Tocilizumab in the form of monotherapy effectively reduced inflammation, while the efficacy of baricitinib for stopping the cytokine storm in monotherapy was insufficient. Based on CT data, both target drugs did not stop the progression of lung lesions on day 5.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42203295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Of Inflammation Gene Polymorphism With Increased Risk Of Metabolic Syndrome In Tatar Ethnic Group","authors":"O. Kochetova, D. Avzaletdinova, G. Korytina","doi":"10.15275/rusomj.2022.0305","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0305","url":null,"abstract":"Background and objective — Chronic low-grade inflammation plays an important role in pathophysiology of metabolic syndrome (MetS). The aim of our study was to determine the associations of polymorphic variants of inflammation genes with MetS and serum levels of high-sensitivity C-reactive protein (hsCRP) and tumor necrosis factor-α (TNF-α) in Tatar patients (Bashkortostan). Methods — In our case-control cross-sectional study, 271 MetS patients and 327 healthy Tatars were genotyped for the SNPs in CRP, TNFA, LTA, TNFRSF1B genes. Results — TNFRSF1B (rs1061624) was associated with the MetS [odds ratio (OR)=0.49, рADJ=0.0034] and TNF-α level (p=0.033). TNFA (rs1800629) was associated with TNF-α (p=0.015), albuminuria (p=0.013). CRP (rs2794521) was associated with fasting (p=0.0096) and postprandial (p=0.01) insulin, HOMA-IR (homeostasis model assessment of insulin resistance, p=0.0019), hsCRP (p=0.036), waist-hip ratio (WHR, p=0.007), body mass index (BMI, p=0.039). The participants having the C-C haplotype of CRP rs2794521-rs1130864 were more common among MetS patients (OR=1.99, p=0.032). T-T haplotype in CRP was associated with hsCRP (p=0.0043), low-density lipoprotein cholesterol (p=0.025), HOMA-IR (p=0.00029), glycated hemoglobin (p=0.006), postprandial (p=0.0006) and fasting insulin (p=0.00031), WHR (p=0.00012), BMI (p=0.00024). Conclusions — The data confirms that the variants of inflammation genes CRP, TNFA, TNFRSF1B are associated with levels of TNF-α, hsCRP. Novel association of TNFRSF1B (rs1061624) with MetS had been identified.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42928877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence Of Sleep Disorders Among Physicians And Nurses During COVID-19 Pandemic","authors":"V. Karnaukhov, E. Narodova, I. Demko, N. Shnayder, Valeriya V. Narodova, D. Dmitrenko, R. Nasyrova","doi":"10.15275/rusomj.2022.0301","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0301","url":null,"abstract":"The objective of our review was to analyze studies of prevalence sleep disorders among medical workers during COVID-19 pandemic. Material and Methods — Overall, during the analyzed period, studying domestic and foreign databases, we identified 94 publications on the development of sleep disorders among medical personnel working during COVID–19 pandemic in 2000-2021. However, only 88 of these publications complied with the objective of the review. We included 75 full-text publications in this review. Results — Physicians and nurses during COVID-19 pandemic do not have the opportunity to take therapy aimed at combating the symptoms of sleep disorders, due to the need to maintain sufficient attention and the risk of developing excessive drowsiness while on duty in a COVID -19 hospital. The latter can have a negative impact on their professional activities. Thus, this problem certainly requires professional psychotherapeutic correction. Unfortunately, not all medical institutions have such specialists. Conclusion — Based on the literature review, we found out that health care workers have a high risk of developing sleep disorders. This can include violations of the circadian rhythm, a decrease in the quality and duration of night sleep, daytime hypersomnia caused by shift work in a hospital. An additional factor affecting the frequency and severity of sleep disorders in healthcare workers (physicians and nurses) is chronic stress, the importance of which has increased during the COVID-19 pandemic. Currently, the above problem has not been completely solved and requires research in this area and the development of new programs to help medical personnel.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44845393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}