Russian Open Medical Journal最新文献_第5页

Associations Of Fatty Acid Composition In Leukocyte Membranes With Systemic Inflammation In Chronic Obstructive Pulmonary Disease Progression 慢性阻塞性肺疾病进展中白细胞膜脂肪酸组成与全身性炎症的关系

IF 0.4

Russian Open Medical Journal Pub Date : 2022-12-20 DOI: 10.15275/rusomj.2022.0401

Y. Denisenko, T. Novgorodtseva, T. Vitkina, V. Knyshova, M. Antonyuk, N. Bocharova, O. Kytikova

{"title":"Associations Of Fatty Acid Composition In Leukocyte Membranes With Systemic Inflammation In Chronic Obstructive Pulmonary Disease Progression","authors":"Y. Denisenko, T. Novgorodtseva, T. Vitkina, V. Knyshova, M. Antonyuk, N. Bocharova, O. Kytikova","doi":"10.15275/rusomj.2022.0401","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0401","url":null,"abstract":"Background — The development of systemic inflammation is a key pathogenetic mechanism in progression of chronic obstructive pulmonary disease (COPD). Fatty acids (FAs) and their oxidized derivatives serve as essential regulators of inflammation. The relationship between systemic inflammation and FA metabolism in COPD is poorly understood. In our research, we focused on examining the FA composition of the leukocyte membrane in COPD and the FA metabolism in association with systemic inflammation. Objective — We examined 137 patients with mild, moderate, or severe COPD. The control group comprised 32 healthy non-smokers. Methods — Blood cytokines and immune cell subpopulations were evaluated by flow cytometry. The FA composition of the leukocyte membranes was analyzed by gas chromatography. The concentrations of eicosanoids (thromboxane B2 (TXB2), leukotriene B4 (LTB4)) in plasma were measured by ELISA. Results — Our results implied systemic inflammation in all patients with COPD. The analysis of the FA composition of leukocyte membrane demonstrated increased level of saturated FAs and n-6 polyunsaturated fatty acids (PUFAs), along with reduced levels of monounsaturated FAs and n-3 PUFAs, in patients with COPD. The TXB2 and LTB4 content was increasing in COPD patients. We established a significant correlation with n-6 PUFAs, immune cells, and cytokines, which was indicative of an important role of FAs in the progress of systemic inflammation in COPD. Conclusion — Thus, FA modification of immune cells in patients with chronic pathologies of the bronchopulmonary system leads not only to disruption of the cell membrane structure, but also to the pathology of immune response regulation, and contributes to the development of the inflammatory process. The latter is a decisive factor in the pathogenesis of COPD.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41646543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Parameters Of Myocardial Electrical Instability In Patients After Myocardial Infarction Comorbid With A Novel Coronavirus Infection (COVID-19) 心肌梗死合并新型冠状病毒感染（新冠肺炎）患者心肌电不稳定性参数

IF 0.4

Russian Open Medical Journal Pub Date : 2022-12-20 DOI: 10.15275/rusomj.2022.0409

A. Tonkoglaz, E. Averyanova, Y. Barmenkova, Maryam A. Yangurazova, M. Lukyanova, V. Oleynikov

{"title":"Parameters Of Myocardial Electrical Instability In Patients After Myocardial Infarction Comorbid With A Novel Coronavirus Infection (COVID-19)","authors":"A. Tonkoglaz, E. Averyanova, Y. Barmenkova, Maryam A. Yangurazova, M. Lukyanova, V. Oleynikov","doi":"10.15275/rusomj.2022.0409","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0409","url":null,"abstract":"Objective — This article aims to assess parameters of myocardial electrical instability and arrhythmic events in patients after myocardial infarction (MI), with and without ST-segment elevation, comorbid/noncomorbid with a novel coronavirus infection (COVID-19) using a long-term electrocardiographic (ECG) monitoring. Methods — The study included 64 subjects: 25 (39%) patients with MI comorbid with COVID-19 (MI+C group) and 39 (61%) patients with MI noncomorbid with a novel coronavirus infection (MI group). The mean age of patients was 54.3±6.8 years. A long-term ECG monitoring for 97.4 (95% CI 77.9-115.2) hours was performed with Astrocard®-Telemetry system (Meditek JSC, Russia), starting from the 4th day of MI. Rhythm and conduction disorders, along with ischemic episodes were recorded; an analysis of ventricular late potentials, heart rate turbulence, and QT dispersion was carried out. Results — There were no differences in the frequency of delayed afterdepolarizations in MI and MI+C groups: 15-28% and 18-33% of patients, respectively. An analysis of turbulence parameters did not reveal statistically significant differences between the groups. Such arrhythmic events as frequent supraventricular extrasystole and life-threatening arrhythmias (ventricular extrasystole of grade 4A and higher sensu B. Lown and M. Wolf) were recorded significantly more often in the MI+C group than in the MI group: 48% vs. 20.5% (p=0.021) and 24% vs. 5.1% (p=0.026), respectively. Conclusion — The novel coronavirus infection (COVID-19) exacerbates myocardial electrophysiological heterogeneity in the acute cardiovascular event and is associated with an increase in clinically significant arrhythmic events.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47604693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predicted Oxaloacetate Activity, Gene Expression And Viability Of Human Dermal Fibroblasts 预测人皮肤成纤维细胞的草酰乙酸活性、基因表达和活力

IF 0.4

Russian Open Medical Journal Pub Date : 2022-12-20 DOI: 10.15275/rusomj.2022.0405

N. Kolotyeva, L. Limareva, F. Gilmiyarova, V. V. Boltovskaya, P. Ilyasov, O. Gusyakova, Vasiliy V. Remizov

{"title":"Predicted Oxaloacetate Activity, Gene Expression And Viability Of Human Dermal Fibroblasts","authors":"N. Kolotyeva, L. Limareva, F. Gilmiyarova, V. V. Boltovskaya, P. Ilyasov, O. Gusyakova, Vasiliy V. Remizov","doi":"10.15275/rusomj.2022.0405","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0405","url":null,"abstract":"Rationale — The impact of metabolic pathway intermediates on biological processes and cell viability is a topic of fundamental research: functional potential of low-molecular weight biologically active compounds as regulators of metabolic processes, signal molecules, transmitters of intra- and intercellular signals are of special interest; however, this field remains poorly studied. Objective — To perform computer modeling of oxaloacetate biological activity and to evaluate its effect on human dermal fibroblast culture. Material and Methods — We predicted biological activity of oxaloacetate using in silico methods. The obtained data demonstrated that oxaloacetate could influence proliferative properties and cell culture viability of dermal fibroblasts. We studied metabolic parameters and enzymatic activity in supernatant and cell culture lysate. Cell viability was assessed via methyl tetrazolium test (MTT). Results — We established that oxaloacetate had a stimulating effect on human dermal fibroblast culture. Conclusion — Our results demonstrated the stimulating role of oxaloacetate in the form of changes in the level of metabolites and activity of enzymes, such as gamma-glutamyl transpeptidase (p=0.0019) and glycerol-3-phosphate dehydrogenase (p<0.0001). We also established that oxaloacetate increased the percentage of viable cells, compared with the control (p=0.028).","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41507558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Coping Strategies In Young Healthy Individuals With Type D Personality 健康青年D型人格个体的应对策略

IF 0.4

Russian Open Medical Journal Pub Date : 2022-12-20 DOI: 10.15275/rusomj.2022.0408

A. Sumin, Ingrid Yu. Prokashko, A. V. Shcheglova

{"title":"Coping Strategies In Young Healthy Individuals With Type D Personality","authors":"A. Sumin, Ingrid Yu. Prokashko, A. V. Shcheglova","doi":"10.15275/rusomj.2022.0408","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0408","url":null,"abstract":"Objective — To study the choice of coping strategies and coping intensity in healthy individuals with type D personality. Material and methods — The study included 98 students of Kemerovo State Medical University (KemSMU), 68 women and 30 men (their mean age was 19.1±2.0 years). All subjects filled out psychological questionnaires to identify type D personality (DS-14) and the choice of coping strategies (Ways of Coping Questionnaire (WCQ) and Coping Strategy Indicator (CSI)). Results — The participants were divided into a group with type D personality (n=44) and without it (n=54). Individuals with type D personality had higher scores on the Escape-Avoidance (p<0.001), Accepting Responsibility (p=0.009) and Distancing (p=0.05) scales of the WSQ questionnaire, and Avoidance strategy scale of the CSI questionnaire (p=0.007). Students with type D personality were characterized by a pronounced preference for the Escape-Avoidance strategy (p=0.000018). An increase of 1 point in the values on the Escape-Avoidance scale improved the chance of identifying type D personality by 1.15 times (p<0.001). An increase in scores on the Positive Reappraisal scale reduced the likelihood of identifying type D personality (OR 0.98; p=0.005). The Escape-Avoidance coping strategy was rather strongly associated with type D personality (AUC=0.779). Conclusion — In healthy young people with type D personality, inadequate coping strategies were notably prevalent: the Escape-Avoidance strategy identified by WCQ, and the Avoidance strategy marked by the CSI. The Escape-Avoidance strategy was independently associated with type D personality, and the Positive Reappraisal strategy was associated with the non-D type.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44283655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Carbohydrate Metabolism, Pancreas Functional Activity And Diet In Young Residents Of Two Ethnic Groups In The North 北方两个民族青年居民碳水化合物代谢、胰腺功能活动与饮食

IF 0.4

Russian Open Medical Journal Pub Date : 2022-12-20 DOI: 10.15275/rusomj.2022.0404

I. Averyanova

{"title":"Carbohydrate Metabolism, Pancreas Functional Activity And Diet In Young Residents Of Two Ethnic Groups In The North","authors":"I. Averyanova","doi":"10.15275/rusomj.2022.0404","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0404","url":null,"abstract":"The objective of this study was to analyze the type and balance of the diet, examine the hormonal regulation of carbohydrate metabolism in order to identify the etiology of hyperglycemic manifestations in young people of two ethnic groups residing in the territory of Magadan Oblast. Methods — Blood serum carbohydrate parameters were measured using the ion-exchange chromatography, enzymatically enhanced chemiluminescence, and the hexokinase method. Results — We established that the homeostatic model assessment of insulin resistance (HOMA-IR) index did not exceed the reference limits, which implied that the examined young men of two ethnic groups had no insulin resistance. The indigenous subjects demonstrated statistically significantly higher values of glycosylated hemoglobin, closely approaching the upper limit of the reference range. The blood insulin level in examined young men was lowered towards the lower limit of the reference range. Against the background of detected hyperglycemic manifestations in young men of two studied groups in Magadan Oblast, their dietary intake was characterized by insufficiency and imbalance in both micronutrient and macronutrient compositions. It was established that the observed diet contained an excessive amount of mono- and disaccharides in 49% of Caucasians and 72% of natives, which was 52% and 108% higher, respectively, compared with the reference range against the pronounced deficiency of chromium, copper, and vitamin D. Conclusion — The results indicated an unbalanced diet with excessive intake of mono- and disaccharides, along with reduced insulin secretion and increased glycosylated hemoglobin, which was more pronounced in the indigenous group, probably causing hyperglycemic manifestations in the carbohydrate metabolism observed in young residents of Magadan Oblast.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44577657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship Between Cardio-Ankle Vascular Index And Coronary Artery Calcification In A Population Sample Of Southwestern Siberia 西伯利亚西南部人群心踝血管指数与冠状动脉钙化的关系

IF 0.4

Russian Open Medical Journal Pub Date : 2022-12-20 DOI: 10.15275/rusomj.2022.0410

A. Sumin, A. V. Shcheglova, A. Kokov, E. N. Kachurina, O. Barbarash

{"title":"Relationship Between Cardio-Ankle Vascular Index And Coronary Artery Calcification In A Population Sample Of Southwestern Siberia","authors":"A. Sumin, A. V. Shcheglova, A. Kokov, E. N. Kachurina, O. Barbarash","doi":"10.15275/rusomj.2022.0410","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0410","url":null,"abstract":"Objective — To examine associations between cardio-ankle vascular index (CAVI) and coronary artery calcium (CAC) score a population sample of southwestern Siberia. Methods — From the sample of 1,620 people the final analysis included 1,316 participants 25 to 64 years of age who were enrolled in an observational cross-sectional study, Epidemiology of Cardiovascular Diseases and Their Risk Factors in the Russian Federation (ESSE-RF). Study participants were split among two groups: Group 1 with CAVI≥9.0 (n=128) and Group 2 with CAVI<9.0 (n=1,188). Prevalence of coronary artery calcification in both groups was analyzed via the Agatston method. We compared main demographic and clinical data between the groups, as well as CAC scores. Results — Elevated CAVI (≥9.0) was present in 9.7% of people included in a population sample from southwestern Siberia, and coronary artery calcification was found in 33.5% of the sample. While similar rates of minimum, mild, moderate and severe CAC score were observed in the participants with elevated and normal CAVI values, CAVI as a continuous variable was statistically significantly associated with moderate and severe CAC scores (OR 1.20, 95% CI 1.06-1.37, p=0.004). CAC score values were higher in individuals at the age of ≥50 years with pathological CAVI values (116±489 vs. 75±425 in normal CAVI, p=0.035), but not in patients under the age of 50 years (64±227 and 85±475, p=0.343). Conclusion — CAVI could possibly be used as a feasible marker before assessing the CAC score in some asymptomatic Caucasian subjects, but identifying the most appropriate methods and participants, whom it could be clearly applicable to, requires further studying.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44205183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Examining Apoptotic Activity Of Gratiola Officinalis L. (Scrophulariaceae) Extract On Cultured Human Tumor Cell Lines 葛藤提取物对培养的人肿瘤细胞凋亡活性的研究

IF 0.4

Russian Open Medical Journal Pub Date : 2022-12-20 DOI: 10.15275/rusomj.2022.0415

N. Polukonova, N. Navolokin, M. Baryshnikova, G. Maslyakova, A. Bucharskaya, A. Polukonova

{"title":"Examining Apoptotic Activity Of Gratiola Officinalis L. (Scrophulariaceae) Extract On Cultured Human Tumor Cell Lines","authors":"N. Polukonova, N. Navolokin, M. Baryshnikova, G. Maslyakova, A. Bucharskaya, A. Polukonova","doi":"10.15275/rusomj.2022.0415","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0415","url":null,"abstract":"Objective — To investigate the apoptotic activity of Gratiola officinalis L. extract on human tumor cell lines by flow cytofluorometry. Material and Methods — The extract of Gratiola officinalis L. was manufactured via our original methodology. Studies were performed on human tumor cell lines: HeLa – cervical carcinoma, Jurkat – T-cell lymphoblastic leukemia, MCF-7 – breast adenocarcinoma, A549 – lung carcinoma, PC-3 – prostate carcinoma, HCT-116 – colon carcinoma, A498 – renal carcinoma, and SK-BR-3 – human breast carcinoma. Induction of apoptosis was studied after incubating cell lines with Gratiola officinalis L. extract at a concentration of 0.9 mg/mL using the Annexin V-FITC Apoptosis Kit. Caspase-dependent apoptosis was examined on a flow cytometer using anti-caspase-3-FITC (BD) kit on the Jurkat cell line. Morphological studies of HeLa cervical carcinoma cells in the alive and dead test were performed using two stains, acridine orange and propidium iodide, at different concentrations of the extract. The statistical data processing was performed using Microsoft Office Excel software. Results — One day after their exposure to Gratiola officinalis L. extract at a concentration of 0.9 mg/mL, tumor cells were mostly in late apoptosis stage. Cytotoxic activity of Gratiola officinalis L. extract was established for all investigated tumor cell cultures but their sensitivities to the extract were different. Mechanisms of antitumor action of Gratiola officinalis L. extract were identified: we established that the extract induced caspase-dependent apoptosis in tumor cells. Conclusion — The identified mechanisms of apoptotic activity of Gratiola officinalis L. extract confirmed the prospects of bioflavonoids as new-generation antitumor agents.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48659958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Role Of P-Selectin, Hemopexin, Lactoferrin, Iron And Ferritin In Patients With Giardiasis And Amoebiasis: A Narrative Review P-选择素、血红素、乳铁蛋白、铁和铁蛋白在贾第虫病和阿米巴病患者中的作用：叙述性综述

IF 0.4

Russian Open Medical Journal Pub Date : 2022-09-28 DOI: 10.15275/rusomj.2022.0314

S. K. Al-Hadraawy, A. H. Abood, R. Zabibah, Ameer A. Imarah, A. Almulla, Ali Abbas Abo Algon

引用次数: 1

Efficacy Of Intradermal Allogeneic Fibroblast Injections In Junctional Epidermolysis Bullosa 真皮内异基因成纤维细胞注射治疗大疱性交界性表皮松解症的疗效

IF 0.4

Russian Open Medical Journal Pub Date : 2022-09-28 DOI: 10.15275/rusomj.2022.0315

A. Kubanov, A. Karamova, V. Chikin, E. Monchakovskaya, Mariya A. Nefedova

引用次数: 1

Effect Of Adaptation Duration To Environmental Conditions Of The North On Morphofunctional Restructuring Of Capillary Blood Flow 北方环境适应期对毛细血管血流形态功能重建的影响

IF 0.4

Russian Open Medical Journal Pub Date : 2022-09-28 DOI: 10.15275/rusomj.2022.0304

I. Averyanova, S. Vdovenko

{"title":"Effect Of Adaptation Duration To Environmental Conditions Of The North On Morphofunctional Restructuring Of Capillary Blood Flow","authors":"I. Averyanova, S. Vdovenko","doi":"10.15275/rusomj.2022.0304","DOIUrl":"https://doi.org/10.15275/rusomj.2022.0304","url":null,"abstract":"Background — We studied blood microcirculation and features of capillary morphology in young men with various duration of adaptation to the environmental conditions of Northeast Russia (Magadan Oblast). Methods — We examined 211 young men 17-21 years of age who were permanent residents of the North. All study subjects were divided among four groups: Caucasian migrants from central Russia (generation 0) and those born and residing in the North, representing generations 1, 2, and 3. We investigated capillary structure and microcirculation in the eponychium of the nail bed, using a computer-based video capillaroscope, Capillaroscan-1. Results — We detected the diameter reduction in arterial and intermediate capillary segments with simultaneous absence of a similar pattern in the venous capillary segment along the gradient of generation 0 towards generation 3. We also observed a shorter mean capillary length against the background of a thicker capillary network. Conclusion — With a longer adaptation period to the environmental conditions of Northeast Russia, compensatory adaptive mechanisms in the capillary network structure and microcirculation are formed, aimed at optimizing capillary blood flow.","PeriodicalId":21426,"journal":{"name":"Russian Open Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46813738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0