Revista médica del Instituto Mexicano del Seguro Social最新文献_第9页

[Obtaining blood components in blood banks]. [在血库中获取血液成分]。

Revista médica del Instituto Mexicano del Seguro Social Pub Date : 2023-01-01

Bárbara Novelo-Garza, Gamaliel Benítez-Arvizu

{"title":"[Obtaining blood components in blood banks].","authors":"Bárbara Novelo-Garza, Gamaliel Benítez-Arvizu","doi":"","DOIUrl":"","url":null,"abstract":"After the first successful blood transfusion, different difficulties of a liquid tissue were overcome; this liquid required special conditions to keep its characteristics with minimal alterations and, thus, to be able to be used in patients who needed it. Subsequently, techniques that also made possible to separate this liquid into its different components for its use were discovered, allowing a more specific treatment of the deficiencies of patients when administering cellular or non-cellular elements. With this, a new area arose within the blood banks to obtaining components. This area became the central point of convergence of all the processes involved in obtaining components, which include the biological qualification of each one of the units, as well as their labeling and release for the different distribution in transfusion services. It is important to highlight that the main source of components is obtained from whole blood; its processing for several decades was an artisanal operator-dependent process; however, with the evolution of technology, now it is possible to carry it out in an automated manner; likewise, today it is possible to obtain components directly from the donor's whole blood by separating it in real time by means of apheresis, which allows obtaining the component of interest and returning the remainder to the donor.","PeriodicalId":21419,"journal":{"name":"Revista médica del Instituto Mexicano del Seguro Social","volume":"61 Suppl 1","pages":"S52-S58"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a1/dd/04435117-61-Suppl1-S52.PMC10395912.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40475419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Impact of the analitycal quality for the infectious serology]. [分析质量对感染血清学的影响]。

Revista médica del Instituto Mexicano del Seguro Social Pub Date : 2023-01-01

Gisela Cortés-Rivera

{"title":"[Impact of the analitycal quality for the infectious serology].","authors":"Gisela Cortés-Rivera","doi":"","DOIUrl":"","url":null,"abstract":"The application of quality and its scope have diversified. From this perspective, quality assurance applied to analytical tests (performed in clinical laboratories or blood banks) for the detection of infectious markers is one of the concepts that has gained strength in the last 10 years. The official Mexican standards require ensuring the quality of the published results and for their compliance it is recommended the use of international guides and guidelines that describe good practices that can be applied when carrying out this activity, without losing sight of the fact that everything related to quality assurance must be supported by the implementation of a quality management system. Thus, by using materials selected correctly and in accordance with official regulatory requirements, a good statistical analysis and the appropriate tools, the quality assurance of the analytical phase of the laboratory process in the screening of infectious markers can bring great benefits to the emission of clinically useful results by monitoring indicators and applying the necessary corrective actions, in order to reduce the risk of unfavorable results for patients and donors.","PeriodicalId":21419,"journal":{"name":"Revista médica del Instituto Mexicano del Seguro Social","volume":"61 Suppl 1","pages":"S65-S71"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8d/51/04435117-61-Suppl1-S65.PMC10396046.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40467486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[ABO incompatibility and complications in hematopoietic stem cell transplantation]. [造血干细胞移植中的ABO血型不合和并发症]。

Revista médica del Instituto Mexicano del Seguro Social Pub Date : 2023-01-01

Marco Alejandro Jiménez-Ochoa, María Margarita Contreras-Serratos, Martha Leticia González-Bautista, Constantino López-Macías, Anahí Torres-Fierro, Elizabeth Urbina-Escalante

{"title":"[ABO incompatibility and complications in hematopoietic stem cell transplantation].","authors":"Marco Alejandro Jiménez-Ochoa, María Margarita Contreras-Serratos, Martha Leticia González-Bautista, Constantino López-Macías, Anahí Torres-Fierro, Elizabeth Urbina-Escalante","doi":"","DOIUrl":"","url":null,"abstract":"Background: Hematopoietic stem cell transplants (HSCT) can be performed regardless of the ABO group compatibility between donor and recipient. ABO incompatibility in HSCT is related to pure red cell aplasia (PRCA), or passenger lymphocyte syndrome. The impact of ABO incompatibility on graft-versus-host disease and transplant-related mortality is controversial due to the heterogeneity of procedures carried out in different transplant centers.Objective: To determine the prevalence of ABO incompatibility and its complications in a hematopoietic stem transplant unit.Material and methods: An observational, retrospective study was carried out in patients undergoing HSCT from January 2014 to January 2020. All trasplant patients were included. Qualitative variables were analyzed using chi-squared test, and Wilcoxon and Student's t tests were used for quantitative variables. A p < 0.05 was considered significant.Results: 124 patients undergoing HSCT were analyzed, out of which 31 had ABO incompatibility, with a punctual prevalence of 24.4%; among them, 54% presented with major incompatibility, 32% minor incompatibility and 13% bidirectional incompatibility. Three cases of PRCA were reported. There were no differences in survival at one year in both groups.Conclusions: The ABO incompatibility ant its complications were not related to the increase in mortality. Randomized prospective studies are required to define the role of ABO incompatibility in HSCT prognosis.","PeriodicalId":21419,"journal":{"name":"Revista médica del Instituto Mexicano del Seguro Social","volume":"61 Suppl 1","pages":"S12-S18"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3a/ec/04435117-61-Suppl1-S12.PMC10396064.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40703190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Vital impetus… the blood]. [生命的动力…血液]。

Revista médica del Instituto Mexicano del Seguro Social Pub Date : 2023-01-01

Gamaliel Benítez-Arvizu

引用次数: 0

[Seroprevalence of anti-SARS-CoV-2 IgG antibodies in resident physicians]. [住院医师抗严重急性呼吸系统综合征冠状病毒2型IgG抗体的血清流行率]。

Revista médica del Instituto Mexicano del Seguro Social Pub Date : 2023-01-01

María Guadalupe Velázquez-Vázquez, Paolo Alberti-Minutti, Esmeralda Campos-Aguirre, Gamaliel Benítez-Arvizu

{"title":"[Seroprevalence of anti-SARS-CoV-2 IgG antibodies in resident physicians].","authors":"María Guadalupe Velázquez-Vázquez, Paolo Alberti-Minutti, Esmeralda Campos-Aguirre, Gamaliel Benítez-Arvizu","doi":"","DOIUrl":"","url":null,"abstract":"Background: COVID-19 pandemic spread around the world swiftly; there are several diagnostic strategies available. Health workers, especially medical residents (MR), are a high-risk population for acquiring this infection.Objective: To estimate the seroprevalence of antibodies against SARS-CoV-2 and the associated factors in MR of a third level hospital.Material and methods: 330 MR from different specialties were evaluated with a questionnaire and collection of blood samples for analysis by microparticle chemiluminescent immunoassay. The prevalence of previous infection was defined by seropositivity of these antibodies. Descriptive statistics and concordance between the RT-PCR tests and the presence of anti-SARS-CoV-2 IgG were used.Results: Of 330 MR, 84.5% actively participated in COVID patient care. One out of 3 reported symptoms of COVID-19; in 67.6% the possible site of infection was a hospital setting not associated with the COVID area. Out of 71 symptomatic subjects, 61.9% underwent RT-PCR against SARS-CoV-2; 20 were positive. In 15.8% of the total, the presence of anti-SARS-CoV-2 IgG antibodies was determined. Only 1 out of 3 subjects with a positive PCR had antibodies, and 11.3% of the cases, even with a positive RT-PCR test, did not develop humoral immunity.Conclusions: The seroprevalence was lower than that reported at the national level, potentially due to protection measures. The main risk factor was contact with the virus in areas of the hospital not related to COVID, making it imperative to reinforce security protocols in those spaces.","PeriodicalId":21419,"journal":{"name":"Revista médica del Instituto Mexicano del Seguro Social","volume":"61 Suppl 1","pages":"S28-S32"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1f/cf/04435117-61-Suppl1-S28.PMC10395998.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40685411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[External Quality Control in Immunohematology of National Medical Center's Blood Bank. History and present]. [国家医学中心血库免疫血液学外部质量控制。历史与现状]。

Revista médica del Instituto Mexicano del Seguro Social Pub Date : 2023-01-01

Isabel Castillo-Mercado, Alexis Ignacio Galván-Bobadilla, Ruth Bonilla-Zavala, Elizabeth González-Moreno, José Alberto Sánchez-Cañas, Rita Villegas-Martínez, Fátima Martha Castillo-Albarrán, Gamaliel Benítez-Arvizu

{"title":"[External Quality Control in Immunohematology of National Medical Center's Blood Bank. History and present].","authors":"Isabel Castillo-Mercado, Alexis Ignacio Galván-Bobadilla, Ruth Bonilla-Zavala, Elizabeth González-Moreno, José Alberto Sánchez-Cañas, Rita Villegas-Martínez, Fátima Martha Castillo-Albarrán, Gamaliel Benítez-Arvizu","doi":"","DOIUrl":"","url":null,"abstract":"For the proper functioning of the Immunohematology Area, an External Quality Control was established since 1973 through a program that evaluates the performance of the laboratories of the Blood Bank and transfusion services that carry out immunohematology tests. This program consists of sending panel cells to participating blood banks or services, which are phenotyped erythrocyte samples previously studied as problem cases but whose results are unknown by the participating laboratories. The processes in which the program is of most importance are determination of the ABO group, determination of Rh, performance of the direct and indirect Coombs test, and pre-transfusion compatibility tests. It was carried out an observational and retrospective study of the results obtained in the 2020 period from 104 units participating in the Immunohematology Quality Control Program of the National Medical Center's Blood Bank. A panel of cells was sent for external quality control of immunohematology every 45 days, resulting in 9 panels for each unit in the studied period. Compliance with the program was observed in the general result (79.6%), i.e., there was a decrease in the participation of the registered units. Of a maximum score of 100% to be obtained, it was observed a general result of 95.3% compliance of the participating units. The results obtained confirm the good general training of the immunohematology laboratories of the participating units. Yet, as in any external control program, it becomes clear that obtaining an erroneous result is a risk that can occur in any laboratory.","PeriodicalId":21419,"journal":{"name":"Revista médica del Instituto Mexicano del Seguro Social","volume":"61 Suppl 1","pages":"S59-S64"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c3/92/04435117-61-Suppl1-S59.PMC10399761.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40475420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[PCR for COVID in the transplant protocol]. [移植方案中的新冠肺炎PCR]。

Revista médica del Instituto Mexicano del Seguro Social Pub Date : 2023-01-01

Esmeralda Campos-Aguirre, Julio César Martínez-Álvarez, María Araceli Arrazola-García, Oscar David Arroyo-García, María Guadalupe Delgado-Colín, Miguel Ángel Chávez-Durán, Alexis Ignacio Galván-Bobadilla, Gamaliel Benítez-Arvizu

{"title":"[PCR for COVID in the transplant protocol].","authors":"Esmeralda Campos-Aguirre, Julio César Martínez-Álvarez, María Araceli Arrazola-García, Oscar David Arroyo-García, María Guadalupe Delgado-Colín, Miguel Ángel Chávez-Durán, Alexis Ignacio Galván-Bobadilla, Gamaliel Benítez-Arvizu","doi":"","DOIUrl":"","url":null,"abstract":"Background: Since the beginning of the SARS-CoV-2 pandemic, identifying the COVID-19 pathophysiology not only has been addressed to applying diagnostic tests or preventing through vaccines, but also to the timely detection, especially of patients in risk groups such as those in transplants areas (renal, hematology, etcetera). In the case of these patients, using RT-PCR tests avoids putting them at risk by subjecting them to states of immunosuppression that could aggravate their situation if they were faced with an onset of a COVID-19 infection.Objective: To present the results of patients of a transplant unit tested for SARS-CoV-2.Material and methods: Descriptive, observational, cross-sectional, and retrolective study. Data of results of RT-PCR tests of patients who underwent transplantation from June 2021 to April 2022 in a third level hospital were collected.Results: 755 tests were done to patients who underwent transplantation. 384 (50.8%) were women. Out of all patients, only 73 (9.7%) were positive to SARS-CoV-2.Conclusions: Implementing RT-PCR tests as a transplant protocol to detect SARS-CoV-2 prevents fatal complications due to COVID infection to donors and receptors.","PeriodicalId":21419,"journal":{"name":"Revista médica del Instituto Mexicano del Seguro Social","volume":"61 Suppl 1","pages":"S33-S36"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ad/f0/04435117-61-Suppl1-S33.PMC10395893.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40464659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[History of the 60 years of the 21th Century National Medical Center's Central Blood Bank]. 【21世纪国家医疗中心中央血库60年历程】。

Revista médica del Instituto Mexicano del Seguro Social Pub Date : 2023-01-01

Esmeralda Campos-Aguirre, Gamaliel Benítez-Arvizu

{"title":"[History of the 60 years of the 21th Century National Medical Center's Central Blood Bank].","authors":"Esmeralda Campos-Aguirre, Gamaliel Benítez-Arvizu","doi":"","DOIUrl":"","url":null,"abstract":"The Blood Bank of the 21th Century National Medical Center is an important piece in the field of transfusion medicine at the national level. Its history shows that it has been in constant change to be able to meet the needs in an adequate and safe manner for both donors and patients. This article shows the advances that the Central Blood Bank has had since its inauguration. This bank has areas of Immunohematology, Donors Assistance, Serology, Fractionation and Storage Studies. These areas have been improving constantly and areas dedicated to molecular biology have been implemented, which makes the bank remain a reference center at the national level. The bank has qualified personnel who have also been involved in teaching courses for personnel from other units of the Instituto Mexicano del Seguro Social (Mexican Institute for Social Security), as well as in disseminating research studies carried out within the bank. The Blood Bank of the 21th Century National Medical Center is a pillar in transfusion medicine care at the national level and it is a reference center for specialized studies.","PeriodicalId":21419,"journal":{"name":"Revista médica del Instituto Mexicano del Seguro Social","volume":"61 Suppl 1","pages":"S72-S80"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/65/f6/04435117-61-Suppl1-S72.PMC10395980.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40467487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Antiproliferative, apoptotic, and antimigratory activities of kafirins on cervical cancer-derived cell lines]. [卡菲林对宫颈癌衍生细胞系的抗增殖、凋亡和抗迁移活性]。

Revista médica del Instituto Mexicano del Seguro Social Pub Date : 2023-01-01

Gamaliel Benítez-Arvizu, Tania Patricia Castro-Jácome, Erik Tovar-Pérez, Eugenia Alcántara-Quintana

{"title":"[Antiproliferative, apoptotic, and antimigratory activities of kafirins on cervical cancer-derived cell lines].","authors":"Gamaliel Benítez-Arvizu, Tania Patricia Castro-Jácome, Erik Tovar-Pérez, Eugenia Alcántara-Quintana","doi":"","DOIUrl":"","url":null,"abstract":"Background: Cervical cancer is one of the leading causes of death in women worldwide, both in developed and developing countries. Therefore, effective treatment of cervical cancer with potential anti-tumor drugs is important. However, new treatments inspired by nutritional medicine are needed.Objective: To use the human cervical cancer cell lines HeLa and SiHa to evaluate the antiproliferative, apoptotic, and migratory activity of sorghum (kafirins).Materials and methods: The anticancer effects of the kafirins were examined by counting cells, MTT assays, apoptosis, and migration assays.Results: This investigation showed that sorghum induced growth inhibition of HeLa and SiHa cells at a significant level. The growth inhibition is dose-dependent and irreversible. When HeLa and SiHa cells were treated with sorghum due to the activity of kafirins, morphological changes were observed, which were identified through the formation of apoptopic bodies. And the kafirins at concentrations of 37.5, 75, 150, and 300 μg/mL decreased the migration of HeLa cells and SiHa cells.Conclusion: This paper demonstrates the induction of antiproliferative, apoptotic, and anti-migratory activity in HeLa and SiHa cells by kafirins. Sorghum may be used as a nutraceutical with potential cancer-prevention benefits.","PeriodicalId":21419,"journal":{"name":"Revista médica del Instituto Mexicano del Seguro Social","volume":"61 Suppl 1","pages":"S4-S11"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/41/65/04435117-61-Suppl1-S4.PMC10395951.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40686223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Molecular biology in a blood bank]. [血库中的分子生物学]。

Revista médica del Instituto Mexicano del Seguro Social Pub Date : 2023-01-01

Gamaliel Benítez-Arvizu, Esmeralda Campos-Aguirre

{"title":"[Molecular biology in a blood bank].","authors":"Gamaliel Benítez-Arvizu, Esmeralda Campos-Aguirre","doi":"","DOIUrl":"","url":null,"abstract":"Medicine has had a vertiginous advance in the last two centuries. After the first successful transfusions, transfusional medicine and blood banks arose. The ladder perform vital functions, from donor screening to the studies for the analysis of blood that are carried out before its use for transfusion and the follow-up of patients who receive blood components. Molecular biology is highly relevant in these activities, since it has allowed the reduction of window periods for the detection of diseases transmissible by blood; it has allowed the complete study of the typing of blood groups and HLA molecules, and it has allowed the adequate phenotypic interpretation of patients and donors by being able to have their genotype. The most relevant impact of the implementation of molecular biology techniques was the screening for the detection of transfusion-transmissible diseases in blood donors, which has allowed improving the safety of the components obtained. Molecular biology techniques applied in the study of the donor-recipient have allowed better care of patients who have required a transfusion or transplant. In this work, it is reviewed the importance of molecular biology in blood banks, with which the care for the Instituto Mexicano del Seguro Social beneficiaries has improved, as well as for the blood donors who are mostly not insured.","PeriodicalId":21419,"journal":{"name":"Revista médica del Instituto Mexicano del Seguro Social","volume":"61 Suppl 1","pages":"S46-S51"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ec/e5/04435117-61-Suppl1-S46.PMC10395919.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40685414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0