Bioinformatics and Biomedical Research Journal最新文献

FUNDAMENTALS OF PROTEIN-PROTEIN INTERACTIONS AND THEIR METHODS OF CHARACTERIZATION 蛋白质相互作用的基本原理及其表征方法

Bioinformatics and Biomedical Research Journal Pub Date : 2022-08-10 DOI: 10.11594/bbrj.04.02.04

K. A. Audah

引用次数: 0

ANALYSIS OF MICROBIOME DIVERSITY IN CANCER 癌症中微生物组多样性分析

Bioinformatics and Biomedical Research Journal Pub Date : 2022-07-22 DOI: 10.11594/bbrj.04.02.03

N. N. Azizah

引用次数: 0

THE ANTIBACTERIAL ACTIVITY OF BETEL (Piper battle L.) AND BASIL (Ocimum sanctum L.) LEAVES INFUSION AS ANTISEPTIC PREPARATIONS AGAINST SOME BACTERIA IN VITRO 槟榔的抑菌活性研究罗勒(Ocimum sanctum L.)叶片浸剂作为体外抗菌制剂的研究

Bioinformatics and Biomedical Research Journal Pub Date : 2022-07-22 DOI: 10.11594/bbrj.04.02.02

Riesy Nor Fatimah, L. Budiarti, Ghea Medika

{"title":"THE ANTIBACTERIAL ACTIVITY OF BETEL (Piper battle L.) AND BASIL (Ocimum sanctum L.) LEAVES INFUSION AS ANTISEPTIC PREPARATIONS AGAINST SOME BACTERIA IN VITRO","authors":"Riesy Nor Fatimah, L. Budiarti, Ghea Medika","doi":"10.11594/bbrj.04.02.02","DOIUrl":"https://doi.org/10.11594/bbrj.04.02.02","url":null,"abstract":"Betel leaves (Piper battle L.) and basil leaves (Ocimum sanctum L.) are parts of the plant that can be made as antiseptic preparations because they contain antibacterial compounds such as flavonoids, tannins, and saponins. Antiseptic activity can be observed based on its effect in inhibiting the colonization of standard test bacteria of the laboratory, namely Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi as well as the magnitude of the phenol coefficient value. The study aimed to analyze the infusion activity of betel and basil leaves as antiseptic preparations against some test bacteria. This study in vitro used a posttest-only control group design. The infusion treatment of betel and basil leaves is tested in the form of a single preparation and a combination with concentrations of 50%, 75%, and 100% (w/v). The results showed there was a difference in the infusion activity of betel leaves and basil leaves in a single stock and a combination of the number of bacterial colonies tested; the effect of the treatment produces a small number of bacterial colonies in Gram-positive bacteria; 100% combination treatment (b/v) provides an inhibitory effect that is not significantly different from 70% alcohol control against all test bacteria except S.typhi: and obtained the phenol coefficient value from the combination treatment of betel and basil leaves infusion higher against Gram-positive bacteria. The conclusion of this study, the combination of betel and basil leaves infusion has antibacterial activity as an antiseptic preparation that can inhibit the growth of some test bacteria. \u0000 \u0000Keywords: antiseptic preparations, infusion leaves, Piper battle L, Ocimum sanctum L, number of colonies, phenol coefficient","PeriodicalId":214181,"journal":{"name":"Bioinformatics and Biomedical Research Journal","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130930560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

ANTIBACTERIAL ACTIVITY OF EXTRACT COMBINATION OF LEAVES AND PEELS KAFFIR LIME (Citrus Hystrix DC.) AGAINST SOME TEST BACTERIA 青柠叶和果皮提取物组合的抗菌活性研究对抗一些测试细菌

Bioinformatics and Biomedical Research Journal Pub Date : 2022-07-22 DOI: 10.11594/bbrj.04.02.01

Rizky Aulia Madani, L. Budiarti, Erida Wydiamala

{"title":"ANTIBACTERIAL ACTIVITY OF EXTRACT COMBINATION OF LEAVES AND PEELS KAFFIR LIME (Citrus Hystrix DC.) AGAINST SOME TEST BACTERIA","authors":"Rizky Aulia Madani, L. Budiarti, Erida Wydiamala","doi":"10.11594/bbrj.04.02.01","DOIUrl":"https://doi.org/10.11594/bbrj.04.02.01","url":null,"abstract":"Leaf and peel of the kaffir lime plant (Citrus hystrix D.C.) can be used as herbal preparations to treat infections by opportunistic bacteria. The purpose of this study was to analyze the antibacterial activity of the combination of leaf and peel extract against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa bacteria isolates. This study used a posttest-only control group design, including phytochemical tests and in vitro antibacterial activity tests. The combination of leaf and peel extract of kaffir lime tested was 12.5%, 25%, 50%, and 75% (w/v). The results of phytochemical tests on the leaf extract and rind of limes were found to have antibacterial compounds, namely flavonoids, tannins, phenols, and alkaloids. The results of the antibacterial activity test used the paper disc diffusion method, showed that there were differences in the size of the inhibition zone for the four test bacteria; The combination treatment of 75% (1:1) leaf-peel extract gave the greatest inhibitory zone effect on the four test bacteria and was equivalent to the positive control. The conclusion of the study, the combination of leaf and peel extract had different antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa.","PeriodicalId":214181,"journal":{"name":"Bioinformatics and Biomedical Research Journal","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115398853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Arbutin from Leaves and Fruits of Buni (Antidesma Bunius L. Spreng) As Tyrosinase Enzym Inhibitor 从布尼(Antidesma Bunius L. spring)叶和果中分离酪氨酸酶抑制剂熊果苷

Bioinformatics and Biomedical Research Journal Pub Date : 2021-11-29 DOI: 10.11594/bbrj.04.01.02

Gea Ocktiah Palensina, Leni Rosita, Zuraida Sagala

{"title":"Isolation of Arbutin from Leaves and Fruits of Buni (Antidesma Bunius L. Spreng) As Tyrosinase Enzym Inhibitor","authors":"Gea Ocktiah Palensina, Leni Rosita, Zuraida Sagala","doi":"10.11594/bbrj.04.01.02","DOIUrl":"https://doi.org/10.11594/bbrj.04.01.02","url":null,"abstract":"Several studies have shown that plant extractive substances have the potential as active compounds inhibiting the enzyme tyrosinase. Arbutin is an enzyme inhibitor of tyrosinase, known as a popular whitening agent used in cosmetics because of its effectiveness in overcoming skin hyperpigmentation. The purpose of this study was to conduct a qualitative and quantitative analysis of arbutin on Buni Leaves and Fruits (Antidesma bunius L. Spreng). The raw simplicia used are mature and young buni leaves, green, red and purple buni fruits. The extraction method is maceration using methanol as solvent. The initial screening for arbutin content was carried out using thin layer chromatography (TLC) and dichlormethan:methanol 50:50 used as mobile phase. Isolation of arbutin content was carried out using Preparative TLC with the same eluent. Qualitative and quantitative analysis were performed using High Pressured Liquid Chromatography (HPLC) with mobile phase of acetonitrile: water 60:40. The tyrosinase enzyme inhibition activity test was then carried out in vitro using 96-well microplate, l-tyrosine and l-dopa were used as substrate at a wavelength of 492 nm. The Rf values obtained ??for mature buni leaves and green buni fruits, respectively 0.61 and 0,62. The retention time of HPLC chromatogram respectively 2,784 minutes and 2,758 minutes. Arbutin levels in leaves and fruits are 7.9 mg / g and 2 mg / g. The activity of the enzyme tyrosinase of mature buni leaves on L-dopa and L-tyrosine substrate were respectively stated as IC50 values ??of 88.7191ppm and 101.33347 ppm. The activity of the enzyme tyrosinase of the green buni fruit on L-dopa and L-tyrosine substrate respectively stated IC50 values ??of 198,0293 ppm and 246,1296 ppm.","PeriodicalId":214181,"journal":{"name":"Bioinformatics and Biomedical Research Journal","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116945458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Phenol Coefficient Test Combination Infusion of Cananga odorata – Averrhoa bilimbi L. Against Staphylococcus aureus and Salmonella typhi in Vitro 加楠-牛膝联合输注体外抗金黄色葡萄球菌和伤寒沙门菌的苯酚系数试验

Bioinformatics and Biomedical Research Journal Pub Date : 2021-11-29 DOI: 10.11594/bbrj.04.01.03

L. Budiarti, Erida Wydiamala, Najiya Ulfa

{"title":"Phenol Coefficient Test Combination Infusion of Cananga odorata – Averrhoa bilimbi L. Against Staphylococcus aureus and Salmonella typhi in Vitro","authors":"L. Budiarti, Erida Wydiamala, Najiya Ulfa","doi":"10.11594/bbrj.04.01.03","DOIUrl":"https://doi.org/10.11594/bbrj.04.01.03","url":null,"abstract":"Cananga odorata (kenanga) flowers and Averrhoa bilimbi L. (belimbing wuluh) fruit are plant parts that contain relatively the same antibacterial compounds, namely flavonoids, saponins, and tannins. Both of these plants can be developed as an alternative natural antiseptic preparation, which is made in the form of a combination infusion. The effectiveness of an antiseptic preparation is measured by the coefficient value of the phenol antiseptic substance compared to 5% phenol against several standard test bacteria. This study aims to determine the antibacterial activity of the combination infusion of Cananga odorata flower - Averrhoa bilimbi L. fruit against Staphylococcus aureus ATCC 25923 and Salmonella typhi ATCC 19430 based on the in vitro phenol coefficient test. The phenol coefficient test method was carried out conventionally. The coefficient value close to 1 can be said that the antiseptic preparation has good activity and is equivalent to the phenol coefficient value as a comparison. The results showed that the mean coefficient of the phenol coefficient of the combination infusion of Cananga odorata flower - Averrhoa bilimbi L. fruit was 0.91 against S. aureus and 0.83 against S. typhi. The conclusion of this study, the antibacterial activity of the combination infusion of Cananga odorata flower - Averrhoa bilimbi L. fruit against Staphylococcus aureus obtained a higher phenol coefficient value than Salmonella typhi.","PeriodicalId":214181,"journal":{"name":"Bioinformatics and Biomedical Research Journal","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132910186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The Correlation of Use of Depo Medroxy Progesterone Acetate (DMPA) In-jections with Lipid Profile Levels in Rats 大鼠脂质水平与注射Depo - hydroxy孕酮Acetate (DMPA)的相关性

Bioinformatics and Biomedical Research Journal Pub Date : 2021-11-29 DOI: 10.11594/bbrj.04.01.04

Suratiah Suratiah, Dewa Ayu Ketut Surinati, I. Yasa

{"title":"The Correlation of Use of Depo Medroxy Progesterone Acetate (DMPA) In-jections with Lipid Profile Levels in Rats","authors":"Suratiah Suratiah, Dewa Ayu Ketut Surinati, I. Yasa","doi":"10.11594/bbrj.04.01.04","DOIUrl":"https://doi.org/10.11594/bbrj.04.01.04","url":null,"abstract":"Introduction: Family Planning is a national strategy of Indonesia government to manage the population growth. Depo Medroxyprogesterone Acetate (DMPA) is one of injectable contraceptives most widely used because it is simple and easy to obtain. However, it has various side effects causing imbalance of hormone estrogen, in turns to result in a decrease in HDL (High Density Lipoprotein) and an increase in LDL (Low Density Lipoprotein) which will result in an increase in total cholesterol. It will also affect changes in fat metabolism in human body due to hormonal influences. This results in dyslipidemia and atherosclerosis. Method: The method in this study is an experimental study with a pretest-posttest control group design. Results: The purpose of this study was to determine the relationship between duration of use of DMPA injections with lipid profile levels in mice. The data were analyzed by using the Paired t-test parametric test to compare between treatment groups. This study found that there were significant differences in HDL levels and total cholesterol levels between before and after administration of DMPA injections on the 14th and 35th days. There is a significant relationship between the duration of administration of DMPA injections with HDL levels and total cholesterol levels in mice. However, there was no difference in LDL levels and triglyceride levels between before and after administration of DMPA injection on day of 14 and day of 35, while, there was a significant difference between before and after the 35th day. There is no relationship between duration of administration of DMPA injections with LDL levels, while there is a relationship among mice triglycerides. Conclusions: Administration of DMPA injections for a long time lowers HDL.","PeriodicalId":214181,"journal":{"name":"Bioinformatics and Biomedical Research Journal","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133919455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial Activity of Stenochlaena palustris and Sauropus androgynus in Staphylococcus aureus, Escherichia coli and Candidia albicans 雌雄同体窄螯虾对金黄色葡萄球菌、大肠杆菌和白色念珠菌的抑菌活性

Bioinformatics and Biomedical Research Journal Pub Date : 2021-11-29 DOI: 10.11594/bbrj.04.01.05

L. Budiarti, Isnaini Isnaini, Puteri Dayana, Norma Sari, N. S.

{"title":"Antimicrobial Activity of Stenochlaena palustris and Sauropus androgynus in Staphylococcus aureus, Escherichia coli and Candidia albicans","authors":"L. Budiarti, Isnaini Isnaini, Puteri Dayana, Norma Sari, N. S.","doi":"10.11594/bbrj.04.01.05","DOIUrl":"https://doi.org/10.11594/bbrj.04.01.05","url":null,"abstract":"Stenochlaena palustris and Sauropus androgynus are known to contains antimicrobial substances such as flavonoids, saponins and tannins compounds.The purpose of this study was to analyzes the antimicrobial activity of young and old leaf infusions of S. palustris and S. androgynus leaves against Staphylococcus aureus, Escherichia coli and Candida albicans. Analyze the antibacterial activity of a single preparations with a combination preparation of S.palustris (SP) and S.androgynus (SA) leaves infusion against S.aureus, E.coli and C.albicans. Leaves of S.palustris young part (SP1) taken 0-10 cm from shoots and old parts (SP2) 11-20 cm from shoots, while leaves of S.androgynus young part (SA1) leaves number 1 - 10 from the top and the old part (SA2) leaves number 11-20 from the top. The results showed that a single infusion of SP1 75% and SP2 75%, SA1 90% and SA2 90%, and a combination of SP1 75% and SA1 75%, SP2 75% and SA2 75% have the same activity as ampicillin in S.aureus. Single infusion of SP1 90% and SP2 90%, SA1 90% and SA2 90%, combination of SP1 75% and SA1 80% and the combination of SP2 80% and SA2 60% have the same activity as ciprofloxacin in E. coli. Single infusion of SP1 90% and SP2 90%, and a combination of SP1 80% and SA1 80%, SP2 80% and SA2 80% have the same activity as ketoconazole in C.albicans. The difference in activity due to differences in leaf parts used only occurred in E. coli, whereas in S.aureus and C.albicans (p <0.05).","PeriodicalId":214181,"journal":{"name":"Bioinformatics and Biomedical Research Journal","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116489367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Bioactive Peptides from Tempeh Using Peptidecutter’s Cleavage 利用Peptidecutter的切割技术从豆豉中提取生物活性肽

Bioinformatics and Biomedical Research Journal Pub Date : 2021-08-31 DOI: 10.11594/bbrj.03.02.05

B. Tamam, D. Syah, H. Lioe, M. Suhartono, W. Kusuma, Suratiah

{"title":"Bioactive Peptides from Tempeh Using Peptidecutter’s Cleavage","authors":"B. Tamam, D. Syah, H. Lioe, M. Suhartono, W. Kusuma, Suratiah","doi":"10.11594/bbrj.03.02.05","DOIUrl":"https://doi.org/10.11594/bbrj.03.02.05","url":null,"abstract":"empeh is an Indonesian traditional fermented food with rich nutrition and bioactive components. Rhizopus sp,especially Rhizopus oligosporus), lactic acid bacteria (Lactobacillus sp.), and yeast aremicroorganisms involved in Tempeh fermentation. An interesting offer of Bioinformatics (in silico method) as a supporting tool in molecular biology studies has emerged, such as in protein cleavage. This study utilized PeptideCutter application on ExPASyBioinformatics portal (https://web.expasy.org/peptide_cutter/) to cleave soy proteins glycinin G1, G2, G3, G4,G5,-conglycinin-chain,andchainusingavailable enzymes in the applicationwith twosimulations. Simulation I was conducted using enzyme complex produced by Lactobacillus sp.and Rhizopus oligosporus, whilesimulation II was used enzyme complex produced by Lactobacillus sp., Rhizopus oligosporus, and Klebsiella pneumonia. Simulation I was conducted using enzyme complex produced by Lactobacillus sp. and Rhizopus oligosporus, whilesimulation II was used enzyme complex produced by Lactobacillus sp., Rhizopus oligosporus, and Klebsiella pneumoniae. A total of 58 peptides was found from the simulation I and higher than simulation I (41 peptides). The bioactive peptides by the cleavages using PeptideCuttertool were dominated with dipeptides and only three peptides were in the form of tripeptides, namely Leu-Leu-Phe(glycininG1), Val-Val-Phe(glycininG5), and Arg-His-Lys(-conglycinin-chain). Bioactive peptides with antihypertensive and antidiabetic properties were mostly found in this in silicomethod of soybean cleavage.","PeriodicalId":214181,"journal":{"name":"Bioinformatics and Biomedical Research Journal","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133418821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Identification of Significant Proteins in Coronavirus Disease 2019 Protein-Protein Interaction Using Principal Component Analysis and ClusterONE 利用主成分分析和聚类酮鉴定2019冠状病毒病蛋白相互作用中的重要蛋白

Bioinformatics and Biomedical Research Journal Pub Date : 2021-08-04 DOI: 10.11594/bbrj.03.02.04

Hilmi Farhan Ramadhani, Annisa, W. Kusuma

{"title":"Identification of Significant Proteins in Coronavirus Disease 2019 Protein-Protein Interaction Using Principal Component Analysis and ClusterONE","authors":"Hilmi Farhan Ramadhani, Annisa, W. Kusuma","doi":"10.11594/bbrj.03.02.04","DOIUrl":"https://doi.org/10.11594/bbrj.03.02.04","url":null,"abstract":"Coronavirus Disease 2019 (COVID-19) will cause disease complications and organ damage due to excessive inflammatory reactions if left untreated. Computational analysis of protein-protein interactions can be carried out in various ways, including topological analysis and clustering of protein-protein interaction networks. Topological analysis can identify significant proteins by measuring the most important nodes with centrality measurements. By using Principal Component Analysis (PCA), the types of centrality measures were extracted into the overall centrality value. The study aimed to found significant proteins in COVID-19 protein-protein interactions using PCA and ClusterONE. This study used 57 proteins associated with COVID-19 to obtain protein networks. All of these proteins are homo sapiens organism. The number of proteins and the number of interactions from 57 proteins were 357 proteins and 1686 interactions. The results of this study consisted of two clusters; the best cluster was the first cluster with a lower p-value but had an average overall centrality value that closed to the second clus-ter. There are twenty important proteins in that cluster, and all of these proteins are related to COVID-19. These proteins are expected to be used in the process of discovering medicinal compounds in COVID-19","PeriodicalId":214181,"journal":{"name":"Bioinformatics and Biomedical Research Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130399146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2