IF 5.5 1区农林科学

Rice Pub Date : 2023-12-13 DOI: 10.1186/s12284-023-00673-w

Suk-Hwan Kim, Jungwon Yoon, Hanna Kim, Sang-Ji Lee, Nam-Chon Paek

{"title":"Rice Basic Helix-Loop-Helix 079 (OsbHLH079) Delays Leaf Senescence by Attenuating ABA Signaling","authors":"Suk-Hwan Kim, Jungwon Yoon, Hanna Kim, Sang-Ji Lee, Nam-Chon Paek","doi":"10.1186/s12284-023-00673-w","DOIUrl":"https://doi.org/10.1186/s12284-023-00673-w","url":null,"abstract":"Leaf senescence represents the final phase of leaf development and is characterized by a highly organized degenerative process involving the active translocation of nutrients from senescing leaves to growing tissues or storage organs. To date, a large number of senescence-associated transcription factors (sen-TFs) have been identified that regulate the initiation and progression of leaf senescence. Many of these TFs, including NAC (NAM/ATAF1/2/CUC2), WRKY, and MYB TFs, have been implicated in modulating the expression of downstream senescence-associated genes (SAGs) and chlorophyll degradation genes (CDGs) under the control of phytohormones. However, the involvement of basic helix-loop-helix (bHLH) TFs in leaf senescence has been less investigated. Here, we show that OsbHLH079 delays both natural senescence and dark-induced senescence: Overexpression of OsbHLH079 led to a stay-green phenotype, whereas osbhlh079 knockout mutation displayed accelerated leaf senescence. Similar to other sen-TFs, OsbHLH079 showed a gradual escalation in expression as leaves underwent senescence. During this process, the mRNA levels of SAGs and CDGs remained relatively low in OsbHLH079 overexpressors, but increased sharply in osbhlh079 mutants, suggesting that OsbHLH079 negatively regulates the transcription of SAGs and CDGs under senescence conditions. Additionally, we found that OsbHLH079 delays ABA-induced senescence. Subsequent RT-qPCR and dual-luciferase reporter assays revealed that OsbHLH079 downregulates the expression of ABA signaling genes, such as OsABF2, OsABF4, OsABI5, and OsNAP. Taken together, these results demonstrate that OsbHLH079 functions in delaying leaf yellowing by attenuating the ABA responses.","PeriodicalId":21408,"journal":{"name":"Rice","volume":"16 1","pages":""},"PeriodicalIF":5.5,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138631982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HCC1, a Polygalacturonase, Regulates Chlorophyll Degradation via the Ethylene Synthesis Pathway 聚半乳糖醛酸酶 HCC1 通过乙烯合成途径调节叶绿素降解

IF 5.5 1区农林科学

Rice Pub Date : 2023-12-09 DOI: 10.1186/s12284-023-00675-8

Yongxiang Liao, Bing Xiang, Zhenzhen Xue, Asif Ali, Yong Li, Mengyuan Li, Aiji Wei, Jialu Xin, Daiming Guo, Yingxiu Liao, Yunfeng Tian, Zhixue Zhao, Peizhou Xu, Hongyu Zhang, Xiaoqiong Chen, Yutong Liu, Hao Zhou, Duo Xia, Kangxi Du, Xianjun Wu

{"title":"HCC1, a Polygalacturonase, Regulates Chlorophyll Degradation via the Ethylene Synthesis Pathway","authors":"Yongxiang Liao, Bing Xiang, Zhenzhen Xue, Asif Ali, Yong Li, Mengyuan Li, Aiji Wei, Jialu Xin, Daiming Guo, Yingxiu Liao, Yunfeng Tian, Zhixue Zhao, Peizhou Xu, Hongyu Zhang, Xiaoqiong Chen, Yutong Liu, Hao Zhou, Duo Xia, Kangxi Du, Xianjun Wu","doi":"10.1186/s12284-023-00675-8","DOIUrl":"https://doi.org/10.1186/s12284-023-00675-8","url":null,"abstract":"Chlorophyll degradation is an important physiological process and is essential for plant growth and development. However, how chlorophyll degradation is controlled at the cellular and molecular level remains largely elusive. Pectin is a main component of the primary cell wall, and polygalacturonases (PGs) is a group of pectin-hydrolases that cleaves the pectin backbone and release oligogalacturonide. Whether and how PGs affect chlorophyll degradation metabolism and its association with ethylene (ETH) have not been reported before. Here, we report a novel function of PG in a mutant ‘high chlorophyll content1’ hcc1, which displayed a decrease in growth and yield. Our morphological, biochemical and genetic analyses of hcc1, knockout lines and complementation lines confirm the function of HCC1 in chlorophyll degradation. In hcc1, the PG activity, ETH content and D-galacturonic acid (D-GA) was significantly decreased and showed an increase in the thickness of the cell wall. Exogenous application of ETH and D-GA can increase ETH content and induce the expression of HCC1, which further can successfully induce the chlorophyll degradation in hcc1. Together, our data demonstrated a novel function of HCC1 in chlorophyll degradation via the ETH pathway.","PeriodicalId":21408,"journal":{"name":"Rice","volume":"16 1","pages":""},"PeriodicalIF":5.5,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138563559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and Advances of Third-Generation Hybrid Rice Technology: A Review 第三代杂交水稻技术的建立与进步：综述

IF 5.5 1区农林科学

Rice Pub Date : 2023-12-08 DOI: 10.1186/s12284-023-00670-z

Zhufeng Chen, Jianxin Wu, Xing Wang Deng, Xiaoyan Tang

{"title":"Establishment and Advances of Third-Generation Hybrid Rice Technology: A Review","authors":"Zhufeng Chen, Jianxin Wu, Xing Wang Deng, Xiaoyan Tang","doi":"10.1186/s12284-023-00670-z","DOIUrl":"https://doi.org/10.1186/s12284-023-00670-z","url":null,"abstract":"Rice (Oryza sativa L.) is one of the most important food crops worldwide. The utilisation of heterosis (hybrid vigour) has played a significant role in increasing rice yield and ensuring food supply. Over the past 50 years, the first-generation three-line system based on cytoplasmic male sterility, and the second-generation two-line system based on environment-sensitive genic male sterility (EGMS), have been widely applied in hybrid rice production. However, the three-line system is restricted by the matching relationship among the three parental lines and allows only ~ 2–5% of germplasms to be explored for elite combinations. The environmental sensitivity of EGMS lines has posed serious risks to the production of hybrid seeds. These factors have hindered the development and applications of hybrid rice. Third-generation hybrid rice technology (TGHRT) is based on environment-insensitive genic male sterility, which can effectively overcome the intrinsic problems of the three-line and two-line systems. Since the establishment of TGHRT, numerous findings and innovations have been reported. This paper gives a brief review of traditional hybrid rice technologies and discusses the establishment of TGHRT, technical innovations in TGHRT, and future research that is necessary to promote the wide application of TGHRT in rice production.","PeriodicalId":21408,"journal":{"name":"Rice","volume":"4 1","pages":""},"PeriodicalIF":5.5,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138563550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphate Transporter OsPT4, Ubiquitinated by E3 Ligase OsAIRP2, Plays a Crucial Role in Phosphorus and Nitrogen Translocation and Consumption in Germinating Seed. 磷酸盐转运体 OsPT4 被 E3 连接酶 OsAIRP2 泛素化，在发芽种子的磷和氮转运及消耗中发挥关键作用。

IF 4.8 1区农林科学

Rice Pub Date : 2023-12-06 DOI: 10.1186/s12284-023-00666-9

Yafei Sun, Fang Zhang, Jia Wei, Ke Song, Lijuan Sun, Yang Yang, Qin Qin, Shiyan Yang, Zhouwen Li, Guohua Xu, Shubin Sun, Yong Xue

{"title":"Phosphate Transporter OsPT4, Ubiquitinated by E3 Ligase OsAIRP2, Plays a Crucial Role in Phosphorus and Nitrogen Translocation and Consumption in Germinating Seed.","authors":"Yafei Sun, Fang Zhang, Jia Wei, Ke Song, Lijuan Sun, Yang Yang, Qin Qin, Shiyan Yang, Zhouwen Li, Guohua Xu, Shubin Sun, Yong Xue","doi":"10.1186/s12284-023-00666-9","DOIUrl":"10.1186/s12284-023-00666-9","url":null,"abstract":"Phosphorus (P) and nitrogen (N) are essential macronutrients necessary for plant growth and development. OsPT4 is a high-affinity phosphate (Pi) transporter that has a positive impact on nutrient uptake and seed development. In this study, the expression patterns of different Pi transporter genes in germinating seeds were determined, and the relative expression of OsPT4 was induced in Pi-deficient seeds and gradually increased with the passage of germination time. The analysis of P, N, Pi, and amino acid concentrations in germinating seeds of OsPT4 mutants showed that the OsPT4 mutation caused P and N retention and a continuous reduction in multiple amino acid concentrations in germinating seeds. Transcriptome analysis and qRT-PCR results also indicated that the OsPT4 mutation inhibits the expression of genes related to P and N transportation and amino acid synthesis in germinating seeds. In addition, the paraffin section and TUNEL assay of OsPT4 mutant germinating seeds suggests that OsPT4 mutation causes programmed cell death (PCD) delayed in the aleurone layer and inhibition of leaf outgrowth. Moreover, we also found that OsPT4 was ubiquitinated by OsAIRP2, which is a C3HC4-type RING E3 Ub ligase. Our studies illustrate that OsPT4 plays a crucial role in P and N collaborative translocation and consumption in germinating seeds. It also provides a theoretical basis for the molecules and physiological mechanisms of P and N cross-talk under suppressed Pi uptake conditions.","PeriodicalId":21408,"journal":{"name":"Rice","volume":"16 1","pages":"54"},"PeriodicalIF":4.8,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10697913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138488325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome- and Transcriptome-wide Association Studies to Discover Candidate Genes for Diverse Root Phenotypes in Cultivated Rice 通过全基因组和全转录组关联研究发现栽培稻不同根系表型的候选基因

IF 5.5 1区农林科学

Rice Pub Date : 2023-12-01 DOI: 10.1186/s12284-023-00672-x

Shujun Wei, Ryokei Tanaka, Taiji Kawakatsu, S. Teramoto, Nobuhiro Tanaka, Matthew Shenton, Y. Uga, Shiori Yabe

引用次数: 0

Comparative Transcriptome Combined with Morphophysiological Analyses Revealed Carotenoid Biosynthesis for Differential Chilling Tolerance in Two Contrasting Rice (Oryza sativa L.) Genotypes. 比较转录组结合形态生理分析揭示了两种对照水稻(Oryza sativa L.)抗冷性差异的类胡萝卜素生物合成基因型。

IF 5.5 1区农林科学

Rice Pub Date : 2023-11-25 DOI: 10.1186/s12284-023-00669-6

Peng Zhang, Xiang Wu, Yulin Chen, Guangmei Ji, Xinling Ma, Yuping Zhang, Jing Xiang, Yaliang Wang, Zhigang Wang, Liangtao Li, Huizhe Chen, Yikai Zhang

{"title":"Comparative Transcriptome Combined with Morphophysiological Analyses Revealed Carotenoid Biosynthesis for Differential Chilling Tolerance in Two Contrasting Rice (Oryza sativa L.) Genotypes.","authors":"Peng Zhang, Xiang Wu, Yulin Chen, Guangmei Ji, Xinling Ma, Yuping Zhang, Jing Xiang, Yaliang Wang, Zhigang Wang, Liangtao Li, Huizhe Chen, Yikai Zhang","doi":"10.1186/s12284-023-00669-6","DOIUrl":"10.1186/s12284-023-00669-6","url":null,"abstract":"Early spring cold spells can lead to leaf chlorosis during the rice seedling greening process. However, the physiological and molecular mechanisms underlying the rice greening process under low-temperature conditions remain unknown. In this study, comparative transcriptome and morphophysiological analyses were performed to investigate the mechanisms mediating the responses of the Koshihikari (Kos) and Kasalath (Kas) rice cultivars to chilling stress. According to their growth-related traits, electrolyte leakage, and chlorophyll fluorescence parameters, Kos was more tolerant to low-temperature stress than Kas. Moreover, chloroplast morphology was more normal (e.g., oval) in Kos than in Kas at 17 °C. The comparative transcriptome analysis revealed 610 up-regulated differentially expressed genes that were common to all four comparisons. Furthermore, carotenoid biosynthesis was identified as a critical pathway for the Kos response to chilling stress. The genes in the carotenoid biosynthesis pathway were expressed at higher levels in Kos than in Kas at 17 °C, which was in accordance with the higher leaf carotenoid content in Kos than in Kas. The lycopene β-cyclase and lycopene ε-cyclase activities increased more in Kos than in Kas. Additionally, the increases in the violaxanthin de-epoxidase and carotenoid hydroxylase activities in Kos seedlings resulted in the accumulation of zeaxanthin and lutein and mitigated the effects of chilling stress on chloroplasts. These findings have clarified the molecular mechanisms underlying the chilling tolerance of rice seedlings during the greening process.","PeriodicalId":21408,"journal":{"name":"Rice","volume":"16 1","pages":"52"},"PeriodicalIF":5.5,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10676345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138441172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Genomic Regions for Deep-Water Resistance in Rice for Efficient Weed Control with Reduced Herbicide Use. 水稻抗深水基因区域的鉴定，为减少除草剂用量有效防治杂草提供依据。

IF 5.5 1区农林科学

Rice Pub Date : 2023-11-25 DOI: 10.1186/s12284-023-00671-y

Marina Iwasa, Koki Chigira, Tomohiro Nomura, Shunsuke Adachi, Hidenori Asami, Tetsuya Nakamura, Takashi Motobayashi, Taiichiro Ookawa

{"title":"Identification of Genomic Regions for Deep-Water Resistance in Rice for Efficient Weed Control with Reduced Herbicide Use.","authors":"Marina Iwasa, Koki Chigira, Tomohiro Nomura, Shunsuke Adachi, Hidenori Asami, Tetsuya Nakamura, Takashi Motobayashi, Taiichiro Ookawa","doi":"10.1186/s12284-023-00671-y","DOIUrl":"10.1186/s12284-023-00671-y","url":null,"abstract":"Deep-water (DW) management in rice fields is a promising technique for efficient control of paddy weeds with reduced herbicide use. Maintaining a water depth of 10-20 cm for several weeks can largely suppress the weed growth, though it also inhibits rice growth because the DW management is usually initiated immediately after transplanting. Improving the DW resistance of rice during the initial growth stage is essential to avoid suppressing growth. In this study, we demonstrate a large genetic variation in the above-ground biomass (AGB) after the end of DW management among 165 temperate japonica varieties developed in Japan. Because the AGB closely correlated with plant length (PL) and tiller number (TN) at the early growth stage, we analyzed genomic regions associated with PL and TN by conducting a genome-wide association study. For PL, a major peak was detected on chromosome 3 (qPL3), which includes a gene encoding gibberellin biosynthesis, OsGA20ox1. The rice varieties with increased PL had a higher expression level of OsGA20ox1 as reported previously. For TN, a major peak was detected on chromosome 4 (qTN4), which includes NAL1 gene associated with leaf morphological development and panicle number. Although there was less difference in the expression level of NAL1 between genotypes, our findings suggest that an amino acid substitution in the exon region is responsible for the phenotypic changes. We also found that the rice varieties having alternative alleles of qPL3 and qTN4 showed significantly higher AGB than the varieties with the reference alleles. Our results suggest that OsGA20ox1 and NAL1 are promising genes for improving DW resistance in rice.","PeriodicalId":21408,"journal":{"name":"Rice","volume":"16 1","pages":"53"},"PeriodicalIF":5.5,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10676340/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138441173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRX102 Participates in Root Hairs Tip Growth of Rice. PRX102参与水稻根毛尖生长

IF 5.5 1区农林科学

Rice Pub Date : 2023-11-16 DOI: 10.1186/s12284-023-00668-7

Sunok Moon, Behnam Derakhshani, Yun Shil Gho, Eui-Jung Kim, Su Kyoung Lee, Xu Jiang, Choonseok Lee, Ki-Hong Jung

引用次数: 0

Rice Thematic Special Issue: Beneficial Plant-Microbe Interactions in Rice. 水稻专题特刊：水稻中有益的植物-微生物相互作用。

IF 5.5 1区农林科学

Rice Pub Date : 2023-11-03 DOI: 10.1186/s12284-023-00659-8

Concha Domingo, Blanca San Segundo

引用次数: 0

GWAS Combined with WGCNA of Transcriptome and Metabolome to Excavate Key Candidate Genes for Rice Anaerobic Germination. GWAS与转录组和代谢组的WGCNA联合挖掘水稻厌氧发芽关键候选基因。

IF 5.5 1区农林科学

Rice Pub Date : 2023-10-31 DOI: 10.1186/s12284-023-00667-8

Dandan Li, Kai Liu, Chuanchao Zhao, Siyi Liang, Jing Yang, Ziai Peng, Aoyun Xia, Meng Yang, Lixin Luo, Cuihong Huang, Jiafeng Wang, Ming Huang, Wuming Xiao, Hui Wang, Ling Su, Tao Guo

{"title":"GWAS Combined with WGCNA of Transcriptome and Metabolome to Excavate Key Candidate Genes for Rice Anaerobic Germination.","authors":"Dandan Li, Kai Liu, Chuanchao Zhao, Siyi Liang, Jing Yang, Ziai Peng, Aoyun Xia, Meng Yang, Lixin Luo, Cuihong Huang, Jiafeng Wang, Ming Huang, Wuming Xiao, Hui Wang, Ling Su, Tao Guo","doi":"10.1186/s12284-023-00667-8","DOIUrl":"https://doi.org/10.1186/s12284-023-00667-8","url":null,"abstract":"Direct seeding of rice is a lightweight and simple cultivation method, which can effectively promote rice production. Anaerobic germination tolerance is one of the main traits of rice adaptability to direct seeding. The mining of related genetic loci, analysis of anaerobic traits and screening of tolerance genes provided valuable genetic resources for improving the anaerobic germination ability of direct seeding rice. This study conducted a dynamic genome-wide association study (GWAS) based on coleoptile-related traits of 591 rice natural populations, and a total of 317 SNP sites were detected. Integrated dynamic widely targeted metabolomics analysis, we found that xanthine, L-alanine and GABA may be key biomarkers that are sensitive and respond strongly to hypoxic stress perception. By WGCNA analysis of targeted metabolomics and transcriptomics, a total of 3 modules were obtained that were significantly correlated with the above three marker metabolites, namely dark green, dark gray and light green modules, respectively, and several key structural genes of OsAlaAT1, OsGAD4, OsAAH and Os09g0424600 that may affect hypoxic germination were screened from the 3 modules. Among them, OsAlaAT1 (Os10g0390500), located in Chr10-12877840, which is within the GWAS location range of CVAN3d, is considered to be a more reliable candidate gene. Overall, in addition to providing new insight into the metabolic regulation of L-alanine, GABA and xanthine during hypoxic germination of rice. This study also provided a reference for the basic theoretical research and breeding application research on the related traits of anaerobic germination in direct-seeding rice.","PeriodicalId":21408,"journal":{"name":"Rice","volume":"16 1","pages":"49"},"PeriodicalIF":5.5,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10618154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71426522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rice最新文献