Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria最新文献
Gabriela Hémylin Ferreira Moura, Gleyce Silva do Nascimento, Pollyanne Raysa Fernandes de Oliveira, Vanessa Silva Santana, Ayko Shimabukuro, Yuri Marinho Valença, Cecilia Calabuig, Renata Pimentel Bandeira de Melo, Rinaldo Aparecido Mota
{"title":"Occurrence of Apicomplexa protozoa in wild birds in the Northeast region of Brazil.","authors":"Gabriela Hémylin Ferreira Moura, Gleyce Silva do Nascimento, Pollyanne Raysa Fernandes de Oliveira, Vanessa Silva Santana, Ayko Shimabukuro, Yuri Marinho Valença, Cecilia Calabuig, Renata Pimentel Bandeira de Melo, Rinaldo Aparecido Mota","doi":"10.1590/S1984-29612023020","DOIUrl":"https://doi.org/10.1590/S1984-29612023020","url":null,"abstract":"<p><p>Protozoa of the Apicomplexa phylum are worldwide distributed with capacity to infect endothermic animals. The study of these protozoa in wild birds in Brazil is scarce. This study aimed to evaluate the occurrence of apicomplexan protozoa in wild birds in the Northeast of Brazil. From October to December 2019, brain tissue samples were collected from 71 captive birds from the Wild Animal Screening Center of the Pernambuco State (CETRAS-Tangara) and 25 free-living birds from the Caatinga biome in Rio Grande do Norte, totaling 96 animals (41 species). Brain fragments were subjected to molecular diagnosis by nested PCR for the 18s rDNA gene of Apicomplexa parasites, followed by DNA sequencing. This gene was detected in 25% (24/96) of the samples, and it was possible to perform DNA sequencing of 14 samples, confirming three genera: Isospora, Sarcocystis and Toxoplasma from eight bird species (Amazona aestiva, Coereba flaveola, Egretta thula, Paroaria dominicana, Sporophila nigricollis, Cariama cristata, Columbina talpacoti, Crypturellus parvirostris). The occurrence these coccidia in wild birds provides important epidemiological information for the adoption of preventive measures for its conservation. Future studies are needed to better understand the consequence of Apicomplexa infection in birds in Caatinga and Atlantic Forest biomes.</p>","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"32 2","pages":"e014722"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10124610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9420336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ulysses Carvalho Barbosa, Alessandra Ferreira Dales Nava, José Vicente Ferreira Neto, Cindy Alves Dias, Viviane Costa da Silva, Hugo Guimarães de Mesquita, Raquel Telles de Moreira Sampaio, Wanilze Gonçalves Barros, Emanuelle de Sousa Farias, Tullio Romão Ribeiro da Silva, James Lee Crainey, Wanderli Pedro Tadei, Hector Henrique Ferreira Koolen, Felipe Arley Costa Pessoa
{"title":"Dirofilaria immitis is endemic in rural areas of the Brazilian Amazonas state capital, Manaus.","authors":"Ulysses Carvalho Barbosa, Alessandra Ferreira Dales Nava, José Vicente Ferreira Neto, Cindy Alves Dias, Viviane Costa da Silva, Hugo Guimarães de Mesquita, Raquel Telles de Moreira Sampaio, Wanilze Gonçalves Barros, Emanuelle de Sousa Farias, Tullio Romão Ribeiro da Silva, James Lee Crainey, Wanderli Pedro Tadei, Hector Henrique Ferreira Koolen, Felipe Arley Costa Pessoa","doi":"10.1590/S1984-29612023018","DOIUrl":"https://doi.org/10.1590/S1984-29612023018","url":null,"abstract":"<p><p>The canine filarial parasite Dirofilaria immitis has not been reported in Brazil´s Amazonas state capital, Manaus, for over a century. Here, we report one imported and 27 autochthonous D. immitis infections from a microfilarial survey of 766 domestic dog blood samples collected between 2017 and 2021 in Manaus. An Overall prevalence estimate of 15.44% (23/149) was calculated from our two rural collection sites; a prevalence of 1.22% (4/328) was estimated at our periurban collection site, and an overall prevalence of 0.35% (1/289) was calculated from our two urban clinic collections. Our data suggest that in the urban areas of Manaus, where the parasites are very likely vectored by the same species of mosquito that historically vectored Wuchereria bancrofti (Culex quinquefasciatus), prevalence levels are very low and possibly maintained by an influx from rural areas where sylvatic reservoirs and/or more favorable vector transmission dynamics maintain high prevalences.</p>","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"32 2","pages":"e000223"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10153491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9757173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Milagros Vargas-Hernandez, Carlos Montero-Espinosa, Dunia Sánchez-Villaurrutia, Carlos Antonio Duarte, Gervasio Henrique Bechara, Alier Fuentes-Castillo, Julio Ancisar, José Suárez-Alba, Omar Mosqueda-Lobaina, Marisela Suárez-Pedroso
{"title":"Infestation of rabbits with just-molted adults of the cattle tick Rhipicephalus microplus: biological parameters and efficiency.","authors":"Milagros Vargas-Hernandez, Carlos Montero-Espinosa, Dunia Sánchez-Villaurrutia, Carlos Antonio Duarte, Gervasio Henrique Bechara, Alier Fuentes-Castillo, Julio Ancisar, José Suárez-Alba, Omar Mosqueda-Lobaina, Marisela Suárez-Pedroso","doi":"10.1590/S1984-29612023006","DOIUrl":"https://doi.org/10.1590/S1984-29612023006","url":null,"abstract":"<p><p>In this study, we report for the first time the successful infestation of rabbits with just-molted, unfed adults of Rhipicephalus microplus. Six New Zealand White rabbits were experimentally infested with 20 female and 20 male unfed adult ticks released into plastic chambers fixed on the shaved backs of each host. The attachment and feeding processes were successful. The biological characteristics of the ticks and the occurrence of adverse events in the tick-attachment area were studied. The average engorgement period was 10.7 days, and 33.3% of the engorged females completed the parasitic phase. The average weight of the recovered engorged females was 149.8 mg, with an average egg mass weight of 70.9 mg, a conversion efficiency index of 47.3%, and a hatching percentage of 88.31%. The adverse reactions found in the tick-attachment area were the usual inflammatory responses of the organism to infestation by these ectoparasites; however, it did not prevent the ticks from feeding and completing their life cycle. These data indicate that the infestation of rabbits with just-molted, unfed adult ticks could be a valuable, alternative animal model for rapid and economical evaluation of vaccine candidates and new molecules with acaricidal activity against Rhipicephalus microplus.</p>","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"32 1","pages":"e016122"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10602799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Julia Somavilla Lignon, Simone Chinicz Cohen, Marcia Cristina Nascimento Justo, Louis Du Preez, Carine Glaucia Comarella, Rogerio Akio Nishimaru, Paulo Vinicius Abbade Moreira Souza, Michelli Westphal de Ataíde, Daniel Curvellho de Mendonça Müller, Maurício Veloso Brun, Silvia Gonzalez Monteiro
{"title":"New species of Polystomoides (Monogenoidea: Polystomatidae) parasitizing the urinary bladder of a freshwater turtle in Brazil.","authors":"Julia Somavilla Lignon, Simone Chinicz Cohen, Marcia Cristina Nascimento Justo, Louis Du Preez, Carine Glaucia Comarella, Rogerio Akio Nishimaru, Paulo Vinicius Abbade Moreira Souza, Michelli Westphal de Ataíde, Daniel Curvellho de Mendonça Müller, Maurício Veloso Brun, Silvia Gonzalez Monteiro","doi":"10.1590/S1984-29612023045","DOIUrl":"https://doi.org/10.1590/S1984-29612023045","url":null,"abstract":"<p><p>Trachemys dorbigni is the most abundant freshwater turtle species in Rio Grande do Sul, southern Brazil. Chelonians are known to host a wide variety of pathogens, including viruses, bacteria, hemoparasites and helminths. Among these, nine genera of polystomatid flatworms (Monogenoidea; Polystomatidae) infect freshwater turtles: Apaloneotrema, Aussietrema, Fornixtrema, Manotrema, Pleurodirotrema, Polystomoidella, Polystomoides, Uropolystomoides and Uteropolystomoides. However, little is known about the biology of these parasites in the Neotropical Realm. Through investigative cystoscopy, specimens of Polystomatidae were located inside the urinary bladder of the host T. dorbigni. Retrieved specimens were fixed and stained whole mounts prepared for taxonomic identification. In the present paper, a new species of Polystomoides (Monogenoidea: Polystomatidae) parasitizing the urinary bladder of a freshwater turtle of the species T. dorbigni in Brazil is described. Polystomoides santamariensis n. sp. differs from the congeneric species on the length of the genital spines, which are longer. Given the enormous diversity of freshwater turtles around the world, it is likely that a large number of chelonian polystomatids are still unknown.</p>","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"32 3","pages":"e007823"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10313310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lílian Cristina Macedo, Yuri Willkens, Leandro Maurício Oliveira Silva, Scott Lyell Gardner, Francisco Tiago de Vasconcelos Melo, Jeannie Nascimento Dos Santos
{"title":"\"Revisiting the past\": a redescription of Physaloptera retusa (Nemata, Physalopteridae) from material deposited in museums and new material from Amazon lizards.","authors":"Lílian Cristina Macedo, Yuri Willkens, Leandro Maurício Oliveira Silva, Scott Lyell Gardner, Francisco Tiago de Vasconcelos Melo, Jeannie Nascimento Dos Santos","doi":"10.1590/S1984-29612023016","DOIUrl":"https://doi.org/10.1590/S1984-29612023016","url":null,"abstract":"<p><p>Physaloptera Rudolphi, 1819 is a genus of nematodes that includes approximately 100 species parasitic in vertebrates around the world. From these, approximately 30 occur in the Neotropical region, with nine reported from neotropical reptiles. Physaloptera spp. are recognized by their distinct morphology of the apical end and characters of the reproductive system. However, despite the fact that the morphological characters for species diagnosis have been firmly established, we frequently find identification problems regarding poorly detailed descriptions and poorly preserved specimens. These may lead to taxonomic incongruencies. Physaloptera retusa (Rudolphi, 1819) is the most common species of the genus and has been reported from several species of neotropical reptiles. Based on our reexaminations of nematode specimens identified as P. retusa from different museum collections, we provide a detailed redescription including the type material, voucher specimens and new specimens recovered currently and showed in this study with new morphological data obtained using light and scanning electron microscopy tools.</p>","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"32 2","pages":"e017422"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10062017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9234639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lívia Perles, Wanessa Teixeira Gomes Barreto, Gabriel Carvalho de Macedo, Heitor Miraglia Herrera, Rosangela Zacarias Machado, Marcos Rogério André
{"title":"Neorickettsia sp. in coatis (Nasua nasua) in Brazil.","authors":"Lívia Perles, Wanessa Teixeira Gomes Barreto, Gabriel Carvalho de Macedo, Heitor Miraglia Herrera, Rosangela Zacarias Machado, Marcos Rogério André","doi":"10.1590/S1984-29612023042","DOIUrl":"https://doi.org/10.1590/S1984-29612023042","url":null,"abstract":"<p><p>The genus Neorickettsia comprises trematode-associated bacteria that can cause diseases in animals and humans. Despite detection of Neorickettsia antigens in the intestine of coatis kept in captivity in southern Brazil through immunohistochemistry, the molecular identity of the bacteria in South American procyonids remains elusive. The aim of the present study was to investigate the occurrence of Neorickettsia sp. in blood samples from coatis in central-western Brazil. Between March 2018 and January 2019, animals were captured and recaptured in two areas of the Cerrado (Parque Estadual do Prosa, PEP; and Vila da Base Aérea, VBA) located in the city of Campo Grande, state of Mato Grosso do Sul, central-western Brazil. All captures were performed according to convenience. DNA from 97 blood samples was subjected to nested PCR (nPCR) targeting a fragment of the 16S rRNA gene of Neorickettsia sp. Six samples (3.6%; five from VBA and one from PEP) from different coatis were positive in nPCR based on the 16S rRNA. The sequences obtained (~500 bp) showed ˃ 99% similarity to N. risticii. Phylogenetic analysis clustered the sequences detected in the present study in a clade with N. risticii. This is the first molecular detection of Neorickettsia sp. in coatis in Brazil.</p>","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"32 3","pages":"e006623"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10399552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9940816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Association between chronic Anaplasma marginale and Babesia spp. infection and hematological parameters of taurine heifers.","authors":"Natalia Machado Rahal, Gabriela Bueno Luz, Kauê Rodriguez Martins, Bernardo Garziera Gasperin, Josiane de Oliveira Feijó, André Gustavo Cabrera Dalto, Monique Tomazele Rovani, Rodrigo Casquero Cunha, Marcio Nunes Corrêa","doi":"10.1590/S1984-29612023052","DOIUrl":"https://doi.org/10.1590/S1984-29612023052","url":null,"abstract":"<p><p>The aim of this study was to investigate the association between chronic Anaplasma marginale and Babesia spp. infection and hematological parameters of pregnant and non-pregnant taurine heifers. Blood samples from 94 females were collected on the first day (D-10) of timed artificial insemination (TAI) protocol and on pregnancy diagnosis (D+34). Hematological parameters were determined and compared between pregnant (PG) and non-pregnant (NPG) heifers, and within group at different sampling days. Real-time PCR (qPCR) was used to determine A. marginale and Babesia bovis infection, and for absolute quantification of Babesia spp. between PG and NPG groups. Correlation analysis was performed between the number of gDNA copies (CN) of Babesia spp. and hematological parameters. On D-10, mean hemoglobin concentration was higher for NPG, and hematocrit and total plasma protein were higher on D+34 for both groups. There was no difference in Babesia spp. CN between groups. In the first qPCR, all heifers were positive for A. marginale and B. bovis. Significant correlations were found between hemoglobin and erythrocyte and between hemoglobin and hematocrit (r = 0.8082 and r = 0.3009, respectively). Low levels of A. marginale and Babesia spp. did not affect hematological parameters of chronically infected pregnant and non-pregnant taurine heifers.</p>","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"32 3","pages":"e006423"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10503823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10271902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Afiyah, Maulifa Dhea Fira, Muhammad Browijoyo Santanumurti, Mamdoh Taha Jamal, Arif Muttaqin, Sri Subekti, Putri Desi Wulan Sari
{"title":"Blood glucose profile as a rapid method for observing Koi carp (Cyprinus carpio) health status - case study of ectoparasites in Blitar, Indonesia.","authors":"Afiyah, Maulifa Dhea Fira, Muhammad Browijoyo Santanumurti, Mamdoh Taha Jamal, Arif Muttaqin, Sri Subekti, Putri Desi Wulan Sari","doi":"10.1590/S1984-29612023019","DOIUrl":"https://doi.org/10.1590/S1984-29612023019","url":null,"abstract":"<p><p>Assessment of fish health is one of the efforts of farmers in minimizing losses due to disease. Rapid tests on fish health can be done through blood observations. This study aimed to determine the blood glucose profile of koi carp due to ectoparasite infestation from the level of blood glucose. The results showed that reported parasites from Blitar's koi carp were Trichodina, Dactylogyrus, Gyrodactylus, Myxobolus, Thelohanellus, Ichthyophthirius, and Argulus. Trichodina showed the highest prevalence (100%) in this case while Thelohanellus was the highest intensity level (93.8±16.3). The results of blood glucose level measurement based on parasite infestation levels showed no significant difference (p>0.05) though the health problems caused by parasites in light, medium or heavy infestation. This research also indicated that the blood glucose profile could be used as a rapid method to detect fish health caused by parasites. We suggest that other variables such as nutritional status, life stage or feeding must be conducted to ensure the glucose role in parasite identification as a rapid method for the future work.</p>","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"32 2","pages":"e014622"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10109245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9316642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Emmanuel Hernández-Valdivia, Efraín Islas-Ojeda, Rafael Casillas-Peñuelas, Arturo Valdivia-Flores, Alberto García-Munguía
{"title":"Gastrointestinal parasites in bullfrogs (Lithobates catesbeianus) in aquaculture production units in the Mexican central highlands.","authors":"Emmanuel Hernández-Valdivia, Efraín Islas-Ojeda, Rafael Casillas-Peñuelas, Arturo Valdivia-Flores, Alberto García-Munguía","doi":"10.1590/S1984-29612023038","DOIUrl":"https://doi.org/10.1590/S1984-29612023038","url":null,"abstract":"<p><p>In Mexico, intensive production of bullfrogs is one of the most important aquaculture activities, due to growing demand for their meat. Frogs can be hosts for several parasites that negatively affect their development and health. The objective of this study was to identify the presence of intestinal parasites in bullfrogs in aquaculture production units. Eighteen bullfrogs aquaculture production units were selected, and 20 animals (n=360) from each farm. Fecal samples were obtained by mucosal scraping and processed using the concentration method. The overall prevalence of intestinal parasites was 70.5%, and all farms had frogs infected by some species of parasite. Two species of parasites were identified: Eimeria sp. and Strongyloides sp. Significant differences were found regarding parasite prevalence between males and females (73.8% vs 58.8%) and regarding tibia length (5.5 vs 6.1 cm) and weight (168 vs 187 g) between parasitized and non-parasitized frogs. In conclusion, the present study showed a high prevalence of intestinal parasites, and morphometric alterations (weight, snout-cloaca length, radio-ulna length, tibia length and distance between parotid glands) were identified in the parasitized animals. These results provided useful information that will enable establishment of adequate control measures to help minimize the adverse effects of these parasites.</p>","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"32 2","pages":"e001523"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9759483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eduardo Fellipe Melo Santos Soares, Daniel Falcão Lopes Princisval Carlos, Neide Mara de Menezes Epifanio, Katherina Coumendouros, Yara Peluso Cid, Douglas Siqueira de Almeida Chaves, Diefrey Ribeiro Campos
{"title":"Insecticidal activity of essential oil of Cannabis sativa against the immature and adult stages of Ctenocephalides felis felis.","authors":"Eduardo Fellipe Melo Santos Soares, Daniel Falcão Lopes Princisval Carlos, Neide Mara de Menezes Epifanio, Katherina Coumendouros, Yara Peluso Cid, Douglas Siqueira de Almeida Chaves, Diefrey Ribeiro Campos","doi":"10.1590/S1984-29612023003","DOIUrl":"https://doi.org/10.1590/S1984-29612023003","url":null,"abstract":"<p><p>Essential oil (EO) of Cannabis sativa (C. sativa) was evaluated against the egg, larval, pupal, and adult stages of the flea Ctenocephalides felis felis. The chemical composition of EO was determined by gas chromatography with flame ionization and mass spectrometry. EO mainly comprised γ-elemene (16.2%) and caryophyllene oxide (14.2%) as major compounds. To evaluate the mortality of flea stages in vitro, filter paper tests were performed at different concentrations. EO of C. sativa showed insecticidal activity (100% mortality at the highest concentrations) for flea control at egg, larval, pupal, and adult stages, with lethal concentrations (LC50) of 32.45; 91.61; 466.41 and 927.92 μg/cm2, respectively. EO of C. sativa indicated the potential for the development of ectoparasiticide for veterinary use, especially for fleas in egg and larval stages.</p>","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"32 1","pages":"e015122"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10544535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}