Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria最新文献_第10页

Compatibility of different Metarhizium spp. propagules with synthetic acaricides for controlling Rhipicephalus microplus. 不同绿僵菌繁殖体与合成杀螨剂防治小根头虫的相容性。

Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria Pub Date : 2022-01-01 DOI: 10.1590/S1984-29612022018

Adriani da Silva Carneiro, Emily Mesquita, Laura Nóbrega Meirelles, Vânia Rita Elias Pinheiro Bittencourt, Patrícia Silva Golo

{"title":"Compatibility of different Metarhizium spp. propagules with synthetic acaricides for controlling Rhipicephalus microplus.","authors":"Adriani da Silva Carneiro, Emily Mesquita, Laura Nóbrega Meirelles, Vânia Rita Elias Pinheiro Bittencourt, Patrícia Silva Golo","doi":"10.1590/S1984-29612022018","DOIUrl":"https://doi.org/10.1590/S1984-29612022018","url":null,"abstract":"The inappropriate use of synthetic acaricides has selected resistant Rhipicephalus microplus populations. The present study evaluated the compatibility of different Metarhizium spp. propagules (conidia, blastospores, and microsclerotia) by incubating them with synthetic acaricides (amitraz, deltamethrin, and a combination of cypermethrin, chlorpyrifos, and citronellal) for 1 h, 5 h, 10 h, and 24 h. Conidia and microsclerotia of the tested isolates were usually more tolerant to synthetic acaricides than blastospores. Our study also analyzed the in vitro effect of deltamethrin associated with fungal propagules for controlling a population of R. microplus females that were not susceptible to this synthetic acaricide. The use of entomopathogenic fungi in association with deltamethrin in this tick population caused a greater tick control than did the use of the fungus or the synthetic acaricide separately.","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"31 1","pages":"e018221"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9078514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective control of Rhipicephalus microplus in a dairy cattle herd from different genetic groups. 不同遗传群奶牛群体微头虫的选择控制。

Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria Pub Date : 2022-01-01 DOI: 10.1590/s1984-29612022062

Mariana Fogale de Andrade, Rodrigo Giglioti, Gunta Gutmanis, Bianca Tainá Azevedo, Cristiane Fernandes de Carvalho Fiorin, Anibal Eugênio Vercesi Filho, Luciana Morita Katiki, Cecília José Veríssimo

{"title":"Selective control of Rhipicephalus microplus in a dairy cattle herd from different genetic groups.","authors":"Mariana Fogale de Andrade, Rodrigo Giglioti, Gunta Gutmanis, Bianca Tainá Azevedo, Cristiane Fernandes de Carvalho Fiorin, Anibal Eugênio Vercesi Filho, Luciana Morita Katiki, Cecília José Veríssimo","doi":"10.1590/s1984-29612022062","DOIUrl":"https://doi.org/10.1590/s1984-29612022062","url":null,"abstract":"Alternatives for Rhipicephalus microplus control are needed in the light of its resistance to acaricides. One of the ways to decrease the use of acaricides in a herd is selective control (SC). In the present study, SC was evaluated in a dairy herd consisting of different genetic groups: Holstein, Jersey, crossbreed and Girolando. Ticks were counted in the right anterior third region on around 90 cows, totaling nine evaluations at intervals of 21 days. Commercial pour-on acaricide was applied only when the infestation was greater than or equal to eight ticks larger than 4 mm in the anterior third region. Tick counts were transformed into log10 and analyzed using mixed models. There was significant difference among groups: Holstein had the highest averages of tick numbers, as expected, although 34.3% did not receive tick treatment. In the other groups, SC reduced the use of acaricides by 79.1% for crossbreed, 81.5% for Jersey and 94.9% for Girolando. The criterion used for applying the acaricide successfully kept the tick population under control. The great advantage of SC was savings to the system, without harming the animals, in addition to generate fewer residues in the animals and in the environment.","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"31 4","pages":"e012622"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9079951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Dioctophyme renale in wandering dogs in Pelotas, South of Brasil. 巴西南部佩洛塔斯流浪狗中的双掌香蝇。

Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria Pub Date : 2022-01-01 DOI: 10.1590/S1984-29612022008

Carolina Buss Brunner, Haide Valeska Scheid, Fabiano da Rosa Venancio, Jéssica Line Farias de Lima, Leonardo Schuler Faccini, Eliza Simone Viégas Sallis, Margarida Buss Raffi

{"title":"Dioctophyme renale in wandering dogs in Pelotas, South of Brasil.","authors":"Carolina Buss Brunner, Haide Valeska Scheid, Fabiano da Rosa Venancio, Jéssica Line Farias de Lima, Leonardo Schuler Faccini, Eliza Simone Viégas Sallis, Margarida Buss Raffi","doi":"10.1590/S1984-29612022008","DOIUrl":"https://doi.org/10.1590/S1984-29612022008","url":null,"abstract":"This study aimed to verify the occurrence of Dioctophyme renale in stray dogs in the city of Pelotas, in the state of Rio Grande do Sul, Brazil. The Laboratório Regional de Diagnóstico of the Universidade Federal de Pelotas received 146 wandering dogs for necropsy, sent by the City Hall of Pelotas from March 2012 to January 2020. Among the necropsied animals, seventeen dogs (11.64%) were diagnosed with dioctophymosis. Among these dogs, 11 were parasitized with one specimen in the right kidney, two dogs presented two specimens in the right kidney, and in other two dogs, the parasites were in the abdominal cavity. In one dog, two parasites were found in the left kidney; in another dog, both kidneys were parasitized, with two parasites in the right kidney and one in the left kidney. The data obtained in this study showed that the occurrence of D. renale in stray dogs in the city of Pelotas is high, and D. renale mainly parasitizes the right kidney.","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"31 1","pages":"e016821"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9086426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Ectoparasites of Columbina passerina insularis (Columbiformes) in the National Zoological Park, Havana, Cuba. 古巴哈瓦那国家动物园飞蛾属岛虫的体外寄生虫。

Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria Pub Date : 2022-01-01 DOI: 10.1590/S1984-29612021096

Daniel González-Acuña, Armando Cicchino, Diana Echeverry, Karen Ardiles, Pablo Oyarzún-Ruiz, Sergey Mironov, Lucila Moreno

引用次数: 0

Diversity of Eimeria (Apicomplexa: Eimeriidae) species and risk factors associated in natural infecting calves at the Southern Agreste Microregion in the State of Pernambuco, Brazil. 巴西伯南布哥州南格雷斯特微区艾美耳球虫(顶复合体:艾美耳球虫科)物种多样性及自然感染小牛的相关危险因素

Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria Pub Date : 2022-01-01 DOI: 10.1590/S1984-29612022026

Karlla Keyla Ferreira Dos Santos, Lucia Oliveira de Macedo, Ângela Imperiano da Conceição, Lucas Azevedo Dos Santos, Carla Lopes de Mendonça, Leucio Câmara Alves, Rafael Antonio Nascimento Ramos, Gílcia Aparecida de Carvalho

{"title":"Diversity of Eimeria (Apicomplexa: Eimeriidae) species and risk factors associated in natural infecting calves at the Southern Agreste Microregion in the State of Pernambuco, Brazil.","authors":"Karlla Keyla Ferreira Dos Santos, Lucia Oliveira de Macedo, Ângela Imperiano da Conceição, Lucas Azevedo Dos Santos, Carla Lopes de Mendonça, Leucio Câmara Alves, Rafael Antonio Nascimento Ramos, Gílcia Aparecida de Carvalho","doi":"10.1590/S1984-29612022026","DOIUrl":"https://doi.org/10.1590/S1984-29612022026","url":null,"abstract":"Eimeria species have importance to calves because of the economic losses. The aim of this study was to identify the species of Eimeria that affect calves and the risk factors associated with its natural infection. Fecal samples (387) were collected from dairy farms in the southern Agreste of Pernambuco. The feces were evaluated using the Gordon & Whitlock technique and were cultured in 2.5% potassium dichromate for sporulation of oocysts. Odds ratio (OR) were calculated to assess risk factors. Eimeria spp. were detected in 50.65% (196/387) of the samples. Eleven species were identified, being Eimeria bovis (26.64%; 548/2057), Eimeria zuernii (19.69%; 405/2057) and Eimeria ellipsoidalis (14.49%; 298/2057) those more frequent. Small herds (OR = 1.93), calves aged up to six months (OR = 2.12), absence of manure pit (OR = 7.52), fortnightly cleaning (OR = 4.71), collective calf pens (OR = 3.26), manual milking (OR = 2.16) and absence of veterinary care (OR = 2.28) were considered to be risk factors. The data revealed pathogenic species in more than 50% of the farms. Thus, the importance of adopting sanitary measures to reduce the spread of these protozoa in herds should be done, because of economic losses associated with its infection.","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"31 2","pages":"e002222"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901888/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9078530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hemiurid and lecithasterid digenean trematodes and camallanid and cucullanid nematodes parasitizing flounders collected off the coast of Rio de Janeiro State, Brazil. 寄生在巴西里约热内卢州海岸的比目鱼上的半纲和卵磷脂纲线虫，以及寄生在比目鱼上的camall纲和cucul纲线虫。

Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria Pub Date : 2022-01-01 DOI: 10.1590/S1984-29612022011

Michelle Cristie Gonçalves da Fonseca, Nilza Nunes Felizardo, Eduardo José Lopes Torres, Delir Corrêa Gomes, Marcelo Knoff

{"title":"Hemiurid and lecithasterid digenean trematodes and camallanid and cucullanid nematodes parasitizing flounders collected off the coast of Rio de Janeiro State, Brazil.","authors":"Michelle Cristie Gonçalves da Fonseca, Nilza Nunes Felizardo, Eduardo José Lopes Torres, Delir Corrêa Gomes, Marcelo Knoff","doi":"10.1590/S1984-29612022011","DOIUrl":"https://doi.org/10.1590/S1984-29612022011","url":null,"abstract":"A total of 132 flounder specimens (60 Paralichthys isosceles, 36 Paralichthys patagonicus and 36 Xystreurys rasile) were collected off the coast of the state of Rio de Janeiro, Brazil. The fish were measured, necropsied, and had their organs investigated for hemiurid and lecithasterid digenean trematodes and camallanid and cucullanid nematodes. Taxonomic identification of the parasites was based on morphological and morphometric characters and was conducted using bright-field and scanning electron microscopies. The trematodes Lecithochirium monticellii and Aponurus laguncula were found parasitizing P. isosceles, P. patagonicus and X. rasile while the nematodes Procamallanus (Spirocamallanus) halitrophus and Cucullanus bonaerensis were found parasitizing P. isosceles and X. rasile and P. isosceles, P. patagonicus and X. rasile, respectively. Parasite indices of prevalence, mean intensity, mean abundance, and range of infection, as well as infection site, were evaluated for each parasite species. This study allowed to evidence the first occurrence of P. patagonicus by L. monticellii; X. rasile by A. laguncula and P. (S.) halitrophus; and P. isosceles and P. patagonicus by C. bonaerensis in the Western South Atlantic Ocean.","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"31 1","pages":"e019921"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901887/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10871931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Endoparasite diversity and liver alterations in Hoplerythrinus unitaeniatus and Cichlasoma bimaculatum in a quilombola area in Maranhão, Brazil. 巴西maranh<e:1>地区单一蚤和双斑马鱼体内寄生虫多样性及肝脏变化。

Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria Pub Date : 2022-01-01 DOI: 10.1590/s1984-29612022022

Ladilson Rodrigues Silva, Vitórya Mendes da Silva Monteiro, Izabela Alves Paiva, Juliany Silva Mendes, Greiciene dos Santos de Jesus, Marcelo Victor Rodrigues da Silva, D. C. Bezerra, C. Carreiro, L. S. Ribeiro, V. C. S. Coimbra, N. P. Bezerra

{"title":"Endoparasite diversity and liver alterations in Hoplerythrinus unitaeniatus and Cichlasoma bimaculatum in a quilombola area in Maranhão, Brazil.","authors":"Ladilson Rodrigues Silva, Vitórya Mendes da Silva Monteiro, Izabela Alves Paiva, Juliany Silva Mendes, Greiciene dos Santos de Jesus, Marcelo Victor Rodrigues da Silva, D. C. Bezerra, C. Carreiro, L. S. Ribeiro, V. C. S. Coimbra, N. P. Bezerra","doi":"10.1590/s1984-29612022022","DOIUrl":"https://doi.org/10.1590/s1984-29612022022","url":null,"abstract":"Our aim was to assess endoparasite diversity and liver alterations in Hoplerythrinus unitaeniatus (jeju) and Cichlasoma bimaculatum (acará preto) in a quilombola area in Maranhão, Brazil. For this, 21 H. unitaeniatus and 21 C. bimaculatum were caught in a natural environment and transported to a laboratory. After these had been euthanized, endoparasites were collected and identified. Liver alterations were evaluated histological analysis based on the severity of each lesion: stage I, organ functioning not compromised; stage II, more severe lesions that impair normal functioning of the organs; and stage III, very severe and irreversible lesions. Among the fish evaluated, 71.43% H. unitaeniatus and 61.90% C. bimaculatum were parasitized. Contracaecum sp. was found in both species; while acanthocephalans, only in H. unitaeniatus. The alterations were vacuolization, nucleus in the cell periphery, deformation of the cell outline, melanomacrophage center, hyperemia, cytoplasmic degeneration and nuclear vacuolization. Through calculating a histological alteration index, it was found that 26.19% of the specimens presented lesions in stage I; 38.09% lesions in stage II and 9.52% lesions in stage III. It was concluded that there is high prevalence of Contracaecum sp. and that the liver lesions may be adaptive responses by the fish to endoparasitic infection.","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"32 1","pages":"e000922"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87952613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence of Toxoplasma gondii and Neospora caninum antibodies and risk factors in domiciliated dogs of Manaus, Amazonas, Brazil. 巴西亚马逊州马瑙斯地区犬刚地弓形虫和犬新孢子虫抗体发生及危险因素分析。

Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria Pub Date : 2022-01-01 DOI: 10.1590/S1984-29612022024

Hevila Gabrieli Nascimento de Campos, Herbert Sousa Soares, Sergio Santos de Azevedo, Solange Maria Gennari

{"title":"Occurrence of Toxoplasma gondii and Neospora caninum antibodies and risk factors in domiciliated dogs of Manaus, Amazonas, Brazil.","authors":"Hevila Gabrieli Nascimento de Campos, Herbert Sousa Soares, Sergio Santos de Azevedo, Solange Maria Gennari","doi":"10.1590/S1984-29612022024","DOIUrl":"https://doi.org/10.1590/S1984-29612022024","url":null,"abstract":"The presence of antibodies anti-Toxoplasma gondii and Neospora caninum have been described in dogs from virtually all Brazilian states, however in the state of Amazonas, there are few studies on these coccidia. In this study the occurrence of antibodies against T. gondii and N. caninum and risk factors were determined in domiciliated dogs of Manaus, AM. Blood samples were collected from 154 dogs and, during the harvest, a questionnaire was applied with questions related to the animals. The samples were analyzed, for the presence of anti-T. gondii and N. caninum antibodies, by indirect fluorescence antibody test, with cutoff of 16 and 50, respectively. Associations between the variables studied and the presence of antibodies were made by chi-square test, fisher's exact test or G test (p<0.05). Of the 154 samples, 19 (12.3% 95% CI = 7.1% - 17.5%) were reagents to T. gondii, and association (p <0.05) was observed between the presence of antibodies and contact with other dogs. The occurrence of dogs reactive to N. caninum was 1.9% (95% CI = 0.4% - 5.6%) with 3 of the 154 dogs positives, and no association (p>0.05) was observed between the presence of N. caninum antibodies, and the variables studied.","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"31 2","pages":"e020321"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9431088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of a one-tube nested real-time PCR assay for the detection of Cryptosporidium spp. in avian fecal samples. 禽粪隐孢子虫单管巢式实时PCR检测方法的验证。

Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria Pub Date : 2022-01-01 DOI: 10.1590/S1984-29612022017

Bruna Nicoleti Santana, Elis Domingos Ferrari, Alex Akira Nakamura, Giane Serafim da Silva, Marcelo Vasconcelos Meireles

{"title":"Validation of a one-tube nested real-time PCR assay for the detection of Cryptosporidium spp. in avian fecal samples.","authors":"Bruna Nicoleti Santana, Elis Domingos Ferrari, Alex Akira Nakamura, Giane Serafim da Silva, Marcelo Vasconcelos Meireles","doi":"10.1590/S1984-29612022017","DOIUrl":"https://doi.org/10.1590/S1984-29612022017","url":null,"abstract":"The aim of this study was to validate a one-tube nested real-time PCR assay followed by genetic sequencing to detect and identify Cryptosporidium species and genotypes in birds. A total of 443 genomic DNA extracted from avian fecal samples were analyzed by one-tube nested real-time PCR and conventional nested PCR. By one-tube nested real-time PCR, 90/443 (20.3%) samples were positive for Cryptosporidium spp. In contrast, 36/443 (8.1%) samples were positive for Cryptosporidium spp. by conventional nested PCR. The analytical sensitivity test showed that one-tube nested real-time PCR detects approximately 0.5 oocyst (2 sporozoites) per reaction. An evaluation of analytical specificity did not reveal amplification of microorganisms that commonly present nonspecific amplification with primers used for the diagnosis of Cryptosporidium spp. The repeatability analysis showed the same result in 27 out of 30 samples (90%). As for the reproducibility of one-tube nested real-time PCR, 24 of the 30 samples examined (80%) showed the same result. All the 90 samples amplified by one-tube real-time nested PCR were successfully sequenced, leading to the identification of C. baileyi, C. galli, C. meleagridis, C. proventriculi, and Cryptosporidium avian genotype I. Genetic sequencing of conventional nested PCR amplicons was successful in 10/36 (27.8%) of positive samples.","PeriodicalId":21270,"journal":{"name":"Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria","volume":"31 1","pages":"e000522"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9447465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Dung avoidance behavior in Crioula Lanada lambs naturally infected with gastrointestinal nematodes in a rotational pasture system. 轮牧系统中自然感染胃肠道线虫的Crioula Lanada羔羊的避粪行为

Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria Pub Date : 2022-01-01 DOI: 10.1590/S1984-29612022012

Patrizia Ana Bricarello, Leticia Rodrigues Costa, Cibele Longo, Jaqueline Seugling, César Cristiano Basseto, Alessandro Francisco Talamini do Amarante, Maria José Hötzel

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引用次数: 4