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Production and action pattern of inulinase from Aspergillus Niger-245: hydrolysis of inulin from several sources 黑曲霉-245菊粉酶的生产和作用模式:几种来源菊粉的水解
Revista De Microbiologia Pub Date : 1998-10-01 DOI: 10.1590/S0001-37141998000400013
V. Cruz, J. Belote, Márcia Zilioli Belline, R. Cruz
{"title":"Production and action pattern of inulinase from Aspergillus Niger-245: hydrolysis of inulin from several sources","authors":"V. Cruz, J. Belote, Márcia Zilioli Belline, R. Cruz","doi":"10.1590/S0001-37141998000400013","DOIUrl":"https://doi.org/10.1590/S0001-37141998000400013","url":null,"abstract":"A strain of Aspergillus niger isolated from soil samples showed great capacity to produce extracellular inulinase. Although the enzyme has been synthesized in presence of monosaccharides, sucrose and sugar cane molasse, the productivity was significantly higher (p<0.05) when the microorganism was inoculated in media formulated with dahlia extract and pure inulin, as carbon sources. With regard to the nitrogen source, the best results were obtained with casein and other sources of proteic nitrogen, comparatively to the mineral nitrogen. However, statistic significance (p<0.01) only was found between the productivity obtained in the medium prepared with casein and ammonium sulphate. The optimum pH of the purified enzyme for inulin hydrolysis was found between 4.0 and 4.5 and the optimun temperature at 60oC. When treated by 30 minutes in this temperature no loss of activity was observed. The enzyme showed capacity to hydrolyse sucrose, raffinose and inulin from which it liberated only fructose units showing, therefore, an exo-action mechanism. Acting on inulins from several sources, the enzyme showed larger hydrolysis speed on the polissaccharide from chicory (Cichorium intibus), comparatively, to the inulins from dahlia (Dahlia pinnata) and Jerusalem artichoke (Helianthus tuberosus) roots.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"431 2 1","pages":"301-306"},"PeriodicalIF":0.0,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77867734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 48
Structure-biological activity relationship of synthetic trihydroxilated chalcones 合成三羟基查尔酮的结构-生物活性关系
Revista De Microbiologia Pub Date : 1998-09-09 DOI: 10.1590/S0001-37141998000400014
C. Devia, N. Pappano, N. Debattista
{"title":"Structure-biological activity relationship of synthetic trihydroxilated chalcones","authors":"C. Devia, N. Pappano, N. Debattista","doi":"10.1590/S0001-37141998000400014","DOIUrl":"https://doi.org/10.1590/S0001-37141998000400014","url":null,"abstract":"A atividade bacteriostatica de 2’,4’,2-trihidroxichalcona, 2’,4’,3-trihidroxichalcona e 2’,4’,4-trihidroxichalcona, preparadas por condensacao de 2,4-dihidroxiacetofenona e benzaldeido convenientemente substituido, contra Staphylococcus aureus ATCC 25923 foi avaliada pela tecnica de difusao em placas. Importantes halos de inibicao foram observados para os tres compostos. Com o proposito de esclarecer a relacao estrutura-atividade biologica, as concentracoes inibitorias minimas (CIM) frente a S. aureus foram determinadas, empregando o metodo da diluicao em caldo. Os resultados obtidos foram semelhantes a 2’,4’-dihidroxichalcona, observando-se a seguinte ordem: CIM 2’,4’,3-(OH)3 > CIM 2’,4’-(OH)2 > CIM 2’,4’,4-(OH)3 > CIM 2’,4’,2-(OH). A sequencia obtida mostra que a introducao de um grupo doador de eletrons (OH) no anel aromatico B provoca um aumento da bioatividade, sendo a intensidade dependente da posicao do substituinte -OH.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"31 1","pages":"307-310"},"PeriodicalIF":0.0,"publicationDate":"1998-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84370078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
INTERFERON SELECTIVELY INHIBITS THE SYNTHESIS OF MAYARO VIRUS GLYCOPROTEINS 干扰素选择性抑制马雅罗病毒糖蛋白的合成
Revista De Microbiologia Pub Date : 1998-09-01 DOI: 10.1590/S0001-37141998000300014
D. Ferreira, M. C. Rebello
{"title":"INTERFERON SELECTIVELY INHIBITS THE SYNTHESIS OF MAYARO VIRUS GLYCOPROTEINS","authors":"D. Ferreira, M. C. Rebello","doi":"10.1590/S0001-37141998000300014","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300014","url":null,"abstract":"We have previously observed that interferon (recIFNa2b) blocks the process of morphogenesis of Mayaro virus in TC7 cells (monkey kidney). In this work we show that IFNa inhibits preferentially virus glycoproteins and their precursors, and this effect is probably correlated to the alterations in the morphogenesis process previously observed.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"39 1","pages":"219-221"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74235815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
MICROBIAL ACTIVITY OF DARK RED LATOSOL SAMPLES STORED AT DIFFERENT TEMPERATURES 不同温度下暗红色红土样品的微生物活性
Revista De Microbiologia Pub Date : 1998-09-01 DOI: 10.1590/S0001-37141998000300002
F. Vieira, E. Nahas
{"title":"MICROBIAL ACTIVITY OF DARK RED LATOSOL SAMPLES STORED AT DIFFERENT TEMPERATURES","authors":"F. Vieira, E. Nahas","doi":"10.1590/S0001-37141998000300002","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300002","url":null,"abstract":"The enzymatic activity of soil samples stored at temperatures of 5 to -12oC and at room temperature for 0-32 weeks was determined. While alkaline phosphatase and dehydrogenase activity was decreased compared to control in samples stored at low temperatures, acid phosphatase activity showed no significant change.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"31 1","pages":"159-163"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87185055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
ANTIBIOTIC PRODUCTION BY STREPTOMYCES HYGROSCOPICUS D1.5: CULTURAL EFFECT 吸湿链霉菌d1.5的抗生素生产:培养效果
Revista De Microbiologia Pub Date : 1998-09-01 DOI: 10.1590/S0001-37141998000300003
B. K. Bhattacharyya, S. C. Pal, S. Sen
{"title":"ANTIBIOTIC PRODUCTION BY STREPTOMYCES HYGROSCOPICUS D1.5: CULTURAL EFFECT","authors":"B. K. Bhattacharyya, S. C. Pal, S. Sen","doi":"10.1590/S0001-37141998000300003","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300003","url":null,"abstract":"In an attempt to screen out new potent antibiotic producers from soil, Streptomyces hygroscopicus D1.5 was isolated and was found antagonistic to both bacteria and fungi. It could utilise arginine as nitrogen source and glycerol as carbon source at 0.75 g/l and 11.5 g/l level, respectively for maximum antibiotic yield.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"25 1","pages":"167-169"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89888759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 41
INHIBITION OF MAYARO VIRUS REPLICATION BY PROSTAGLANDIN A1 IN Aedes albopictus CELLS 前列腺素A1对白纹伊蚊细胞MAYARO病毒复制的抑制作用
Revista De Microbiologia Pub Date : 1998-09-01 DOI: 10.1590/S0001-37141998000300013
J. A. Barbosa, M. Rebello
{"title":"INHIBITION OF MAYARO VIRUS REPLICATION BY PROSTAGLANDIN A1 IN Aedes albopictus CELLS","authors":"J. A. Barbosa, M. Rebello","doi":"10.1590/S0001-37141998000300013","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300013","url":null,"abstract":"Prostaglandin A1 (PGA1) inhibits Mayaro virus replication in Aedes albopictus cells at nontoxic doses to uninfected cells. At 10 µg/ml, PGA1 decreases virus production by 90%. The presence of PGA1 during virus adsorption, with no treatment after infection, reduces virus yield by 41%. Antiviral activity is observed even when treatment starts at one or two hours post-infection. However, in cells pre-treated with PGA1 during 24 hours, virus replication is not impaired. Thus, events ocurring during initial stages of infection and after virus adsorption and penetration must be the target of PGA1 action. SDS-PAGE analysis of 35S-methionine labelled proteins shows that PGA1 inhibits the synthesis of viral proteins and induces the synthesis of polypeptides with molecular weight of 70 kDa, 57 kDa and 23 kDa. In cells pre-treated with actinomycin D the induction of those proteins is suppressed. In addition, actinomycin D treatment prevents PGA1antiviral activity, indicating that PGA1-induced stress proteins are probably involved in this mechanism.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"24 1","pages":"213-218"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74244321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
A Preliminary Note on Yeasts Associated With Fecal Pellets of Rodents and Marsupials of Atlantic Forest Fragments in Rio de Janeiro, Brazil 巴西里约热内卢大西洋森林碎片中啮齿动物和有袋动物粪便颗粒中酵母菌的初步研究
Revista De Microbiologia Pub Date : 1998-09-01 DOI: 10.1590/S0001-37141998000300004
J. Abranches, H. N. Nóbrega, P. Valente, L. Mendonça-Hagler, A. Hagler
{"title":"A Preliminary Note on Yeasts Associated With Fecal Pellets of Rodents and Marsupials of Atlantic Forest Fragments in Rio de Janeiro, Brazil","authors":"J. Abranches, H. N. Nóbrega, P. Valente, L. Mendonça-Hagler, A. Hagler","doi":"10.1590/S0001-37141998000300004","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300004","url":null,"abstract":"Leveduras em pelotas fecais de pequenos mamiferos provenientes de fragmentos de Mata Atlântica tiveram contagem media acima de 106 UFC/g. A maioria das 55 especies isoladas foi de ascomicetos fermentativos, com as mais frequentes sendo Debaryomyces hansenii, Pichia membranifaciens e Issatchenkia orientalis, enquanto Rhodotorula mucilaginosa foi a levedura mais frequente de afinidade basidiomicetica.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"41 1","pages":"170-173"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73396433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Frequency of Nonfermentative Gram-Negative Bacilli Isolated from Clinical Materials of Pacients at Universidade Federal do Ceará Hospital Complex - Brazil 巴西联邦大学<e:1>综合医院患者临床材料中分离的非发酵革兰氏阴性杆菌的频率
Revista De Microbiologia Pub Date : 1998-09-01 DOI: 10.1590/S0001-37141998000300006
C. Frota, J. L. Moreira
{"title":"Frequency of Nonfermentative Gram-Negative Bacilli Isolated from Clinical Materials of Pacients at Universidade Federal do Ceará Hospital Complex - Brazil","authors":"C. Frota, J. L. Moreira","doi":"10.1590/S0001-37141998000300006","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300006","url":null,"abstract":"Among one thousand eight hundred and thirty-four Gram-negative bacilli, isolated at Universidade Federal do Ceara hospital complex - Brazil, from January 1995 to February 1996, 456 (24.8%) were Nonfermentative Gram-Negative Bacilli (NFGNB). This study reports their identification to the species level and their frequency as well. Thirteen genera and thirty species were identified and Pseudomonas aeruginosa was the most frequent species (69.95%), followed by Acinetobacter baumannii (5.48%) and by Acinetobacter lwoffii (3.95%). Among the identified P.aeruginosa strains, 94.1% produced pigment but 7.9% of them produced pigment only after being cultivated several times. The frequency of the most species was similar to that reported in the literature.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"75 1","pages":"179-182"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77210535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
ANTAGONISM AGAINST VIBRIO CHOLERAE BY BACTERIAL DIFFUSIBLE COMPOUND IN THE FECAL MICROBIOTA OF RODENTS 啮齿动物粪便微生物群中细菌扩散化合物对霍乱弧菌的拮抗作用
Revista De Microbiologia Pub Date : 1998-09-01 DOI: 10.1590/S0001-37141998000300016
Simone Helena Da Silva, E. Vieira, J. Nicoli
{"title":"ANTAGONISM AGAINST VIBRIO CHOLERAE BY BACTERIAL DIFFUSIBLE COMPOUND IN THE FECAL MICROBIOTA OF RODENTS","authors":"Simone Helena Da Silva, E. Vieira, J. Nicoli","doi":"10.1590/S0001-37141998000300016","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300016","url":null,"abstract":"O aparecimento de halo de inibicao contra o Vibrio cholerae a partir das fezes de ratos Wistar e Fischer nas idades de 10 a 42 dias foi observado usando um teste ex vivo em placa. A frequencia de ratos Wistar apresentando halo aumentou de 0% (10 dias) ate um maximo de 80,0% (29 dias) antes de decair para 53,3% (42 dias). Um perfil similar foi obtido com os ratos Fischer mas com valores inferiores (frequencia maxima de 50,0% no dia 36). Num experimento separado quando ratos Wistar foram alimentados com uma racao deficiente em proteina a frequencia de halo diminuiu drasticamente. Tres morfologias de colonias aparentemente distintas foram isoladas da microbiota fecal dominante: uma bacteria anaerobia facultativa (ANF) e duas anaerobias estritas (ANS). O teste inibitorio ex vivo mostrou halo ao redor das fezes de camundongos isentos de germes monoassociados com a bacteria ANF ou uma das bacterias ANS mas nao para os animais isentos de germes. Apos desafio oral de todos os grupos com o V. cholerae, barreiras permissiva e drastica foram observadas em camundongos associados com as bacterias ANF e ANS, respectivamente. As bacterias ANF e uma ANS usadas em monoassociacao nos desafios in vivo foram identificadas como Escherichia coli e Streptococcus intermedius, respectivamente. O potente antagonismo demostrado pela microbiota intestinal de ratos contra V. cholerae parece devido, em parte, a compostos difusiveis e este fenomeno depende aparentemente da idade, de especie e da nutricao do animal. Esses dados preliminares sugerem tambem que este antagonismo seja devido a mais de um componente bacteriano num instante dado.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"338 1","pages":"228-234"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73906568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Transfer of clindamicyn resistance between Bacteroides fragilis group strains isolated from clinical specimens 临床标本分离的脆弱拟杆菌群菌株间克林霉素耐药性转移
Revista De Microbiologia Pub Date : 1998-09-01 DOI: 10.1590/S0001-37141998000300007
C. B. Carvalho, J. L. Moreira, M. Ferreira
{"title":"Transfer of clindamicyn resistance between Bacteroides fragilis group strains isolated from clinical specimens","authors":"C. B. Carvalho, J. L. Moreira, M. Ferreira","doi":"10.1590/S0001-37141998000300007","DOIUrl":"https://doi.org/10.1590/S0001-37141998000300007","url":null,"abstract":"Clindamycin resistance was trasferred by a conjugation-like process from Bacteroides thetaiotaomicron 52, a multiple antibiotic-resistant strain isolated from clinical specimens, to other Bacteroides species. A possible association between a plasmid detected in the donor strain and clindamycin resistance is discussed.","PeriodicalId":21211,"journal":{"name":"Revista De Microbiologia","volume":"38 1","pages":"183-186"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77129007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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